avidin-biotin.
References
1. Voller, A. et al., Bull. World Health Organ., 53(1),
55-65 (1976).
2. Engvall, E., Methods Enzymol., 70(A), 419-439
(1980).
3. Voller, A., and Bidwell, D.
Bagrodia, S., et al., J. Biol. Chem., 270, 22731
(1995).
17. Brown, J. L., et al., Curr. Biol., 6, 598 (1996).
18. Hill, C. S., et al., Cell, 81, 1159 (1995).
19. Manser, E., et al., Nature, 367, 40 (1994
Andersen, H.S., et al., J. Med. Chem., 45, 4443-4459 (2002).
90. Larsen, S.D., et al., J. Med. Chem., 45, 598-622 (2002).
91. Malamas, M.S., et al., J. Med. Chem., 43, 1293-1310 (2000).
92. Desmarais, S., et al
Total RNA x μl 1 to 20 μg
mRNA x μl 0.3 to 2 μg
Oligo[(dT)24 T7 promotor]65 primer 100 pmol/μl (Vial 6)
or
Oligo(dT)15 primer 200 pmol/μl (Vial 5)
2 μl
2 μl
200 pmol
400 pmol
Water, PCR
Total RNA x μl 1 to 20 μg
mRNA x μl 0.3 to 2 μg
Oligo[(dT)24 T7 promotor]65 primer 100 pmol/μl (Vial 6)
or
Oligo(dT)15 primer 200 pmol/μl (Vial 5)
2 μl
2 μl
200 pmol
400 pmol
Water, PCR
10 200 1398
15 300 1298
20 400 1198
25 500 1098
30 600 998
35 700 898
40 800 798
45 900 698
50 1000 598
• Allow the formamide to reach room temperature
from 4 °C storage temperature by placing it in
Cyclin D1 C7464 595 CCND1 M NP_444284.1 y y y
66 Bcl-2 B3170 596 BCL2 M NP_000624.2 y n/d n/d
67 Bcl-x B9304 598 BCL2L1 P NP_001182.1 y y y
68 Bcl-xL B9429 598 BCL2L1 M NP_001182.1 y y y
278 Filensin F1043 631
Cyclin D1 C7464 595 CCND1 M NP_444284.1 y y y
66 Bcl-2 B3170 596 BCL2 M NP_000624.2 y n/d n/d
67 Bcl-x B9304 598 BCL2L1 P NP_001182.1 y y y
68 Bcl-xL B9429 598 BCL2L1 M NP_001182.1 y y y
278 Filensin F1043 631
10 200 1398
15 300 1298
20 400 1198
25 500 1098
30 600 998
35 700 898
40 800 798
45 900 698
50 1000 598
• Allow the formamide to reach room temperature
from 4 °C storage temperature by placing it in
Cyclin D1 C7464 595 CCND1 M NP_444284.1 y y y
66 Bcl-2 B3170 596 BCL2 M NP_000624.2 y n/d n/d
67 Bcl-x B9304 598 BCL2L1 P NP_001182.1 y y y
68 Bcl-xL B9429 598 BCL2L1 M NP_001182.1 y y y
278 Filensin F1043 631
444502.2,NP_001073617.1 M y n/d n/d
65 Bcl-2 B9804 24224, 12043 Bcl2 NP_058689.1,NP_803129.2 P n/d y y
66 Bcl-2 B3170 596 BCL2 NP_000648.2 M y n/d n/d
67 Bcl-x B9304 598, 24888, 12048 Bcl2l1,BCL2L1 NP_612815.1
444502.2,NP_001073617.1 M y n/d n/d
65 Bcl-2 B9804 24224, 12043 Bcl2 NP_058689.1,NP_803129.2 P n/d y y
66 Bcl-2 B3170 596 BCL2 NP_000648.2 M y n/d n/d
67 Bcl-x B9304 598, 24888, 12048 Bcl2l1,BCL2L1 NP_612815.1
Prion protein P0110 M y y y
63 Bcl-10 B0431 P y y y
64 Seladin S4697 M y n/d n/d
65 B l 2Bcl-2 B9804 P /dn/d y y
66 Bcl-2 B3170 M y n/d n/d
67 Bcl-x B9304 P y n/d n/d
REFERENCES
1. Karmali, M. A. (1989) Clin.
Microbiol. Rev. 2, 15–38.
2. Paton, A. W. and Paton, J. C. (1998)
J. Clin. Microbiol. 36, 598–602.
3. M., Nishiya, Y., Kawamura, Y., and
Shinagawa, K.