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Fuhui Xu et al.
PloS one, 9(9), e106509-e106509 (2014-09-04)
Improved drought tolerance is always a highly desired trait for agricultural plants. Significantly increased drought tolerance in Arabidopsis thaliana (Columbia-0) has been achieved in our work through the suppression of ESKMO1 (ESK1) gene expression with small-interfering RNA (siRNA) and overexpression
Arghavan Solemani Zadeh et al.
Methods and protocols, 2(1) (2019-06-06)
Phage display is a powerful technique for drug discovery in biomedical research in particular for antibody libraries. But, several technical challenges are associated with the selection process. For instance, during the panning step, the successful elution of the phages bound
Felix Wong et al.
Nature communications, 12(1), 2321-2321 (2021-04-21)
Bactericidal antibiotics kill bacteria by perturbing various cellular targets and processes. Disruption of the primary antibiotic-binding partner induces a cascade of molecular events, leading to overproduction of reactive metabolic by-products. It remains unclear, however, how these molecular events contribute to
Kathryn M Maselli et al.
American journal of physiology. Gastrointestinal and liver physiology, 319(2), G212-G226 (2020-07-01)
Short bowel syndrome (SBS) is associated with changes in the intestinal microbiome and marked local and systemic inflammation. There is also a late complication of SBS, intestinal failure associated liver disease (IFALD) in which hepatic steatosis progresses to cirrhosis. Most
Lokender Kumar et al.
PloS one, 14(3), e0214411-e0214411 (2019-03-27)
Antimicrobial biopolymers provide a biodegradable, sustainable, safe, and cheap approach to drug delivery and wound dressing to control bacterial infection and improve wound healing respectively. Here, we report a one-step method of making antimicrobial alginate polymer from sodium alginate and
Evangelia Vogiatzaki et al.
Current biology : CB, 27(19), 2893-2900 (2017-09-26)
Seed production requires the transfer of nutrients from the maternal seed coat to the filial endosperm and embryo. Because seed coat and filial tissues are symplasmically isolated, nutrients arriving in the seed coat via the phloem must be exported to
Amit Srivastava et al.
Bio-protocol, 10(4), e3528-e3528 (2021-03-04)
Anabaena sp. PCC 7120 (hereafter Anabaena) is a model cyanobacterium to study nitrogen fixation, cellular differentiation and several other key biological functions that are analogous in plants. As with any other organism, many genes in Anabaena encode an essential life
Ileana Cochetti et al.
Antimicrobial agents and chemotherapy, 52(4), 1285-1290 (2008-02-21)
This study investigated the genetic organization of erm(B)-carrying transposons of Streptococcus pneumoniae and their distribution in tetracycline-resistant clinical isolates. By comparatively analyzing reference pneumococci carrying erm(B)/tet(M) transposon Tn1545, Tn6003, Tn6002, or Tn3872, we demonstrated a substantial correspondence between Tn1545 and
Jia Zheng et al.
Science (New York, N.Y.), 365(6451), 347-353 (2019-07-28)
Cryptic genetic variation can facilitate adaptation in evolving populations. To elucidate the underlying genetic mechanisms, we used directed evolution in Escherichia coli to accumulate variation in populations of yellow fluorescent proteins and then evolved these proteins toward the new phenotype
R.T. Fraley et al.
Miami Winter Symp., 20, 211-211 (1983)
Nana Li et al.
Microbial drug resistance (Larchmont, N.Y.), 21(3), 292-296 (2015-01-20)
A total of 92 Mycobacterium tuberculosis isolates were collected from patients with pulmonary tuberculosis (TB) in the Zunyi region between 2011 and 2012. Collected isolates were used to determine antibiotic susceptibility patterns against 13 anti-TB drugs: 4 first-line and 9
Mario Mardirossian et al.
Cell chemical biology, 25(5), 530-539 (2018-03-13)
Proline-rich antimicrobial peptides (PrAMPs) internalize into susceptible bacteria using specific transporters and interfere with protein synthesis and folding. To date, mammalian PrAMPs have so far been identified only in artiodactyls. Since cetaceans are co-phyletic with artiodactyls, we mined the genome
Rémy Marcellin-Gros et al.
Marine drugs, 18(2) (2020-01-30)
The democratization of sequencing technologies fostered a leap in our knowledge of the diversity of marine phytoplanktonic microalgae, revealing many previously unknown species and lineages. The evolutionary history of the diversification of microalgae can be inferred from the analysis of
Lokender Kumar et al.
Frontiers in microbiology, 12, 598498-598498 (2021-02-16)
Pseudomonas aeruginosa utilizes the quorum sensing (QS) system to strategically coordinate virulence and biofilm formation. Targeting QS pathways may be a potential anti-infective approach to treat P. aeruginosa infections. In the present study, we define cephalosporins' anti-QS activity using Chromobacterium
Sristy Shikha et al.
Scientific reports, 10(1), 1463-1463 (2020-01-31)
Microbes develop several strategies to survive in the adverse condition such as biofilm formation, attaining non-dividing state, altering drug target or drug, thereby increases the burden of drug dosage. To combat these issues, nanoparticles have shown an alternative approach for
Jonathan M Stokes et al.
Nature methods, 16(4), 303-306 (2019-03-13)
Antibiotic screens typically rely on growth inhibition to characterize compound bioactivity-an approach that cannot be used to assess the bactericidal activity of antibiotics against bacteria in drug-tolerant states. To address this limitation, we developed a multiplexed assay that uses metabolism-sensitive
Chandrasekhar Natarajan et al.
Current protocols in protein science, 101(1), e112-e112 (2020-07-21)
The development of new technologies for the efficient expression of recombinant hemoglobin (rHb) is of interest for experimental studies of protein biochemistry and the development of cell-free blood substitutes in transfusion medicine. Expression of rHb in Escherichia coli host cells
Neuronal erythropoietin overexpression is protective against kanamycin-induced hearing loss in mice.
David Bächinger et al.
Toxicology letters, 291, 121-128 (2018-04-15)
Aminoglycosides have detrimental effects on the hair cells of the inner ear, yet these agents indisputably are one of the cornerstones in antibiotic therapy. Hence, there is a demand for strategies to prevent aminoglycoside-induced ototoxicity, which are not available today.
Tae Sung Kim et al.
Scientific reports, 7(1), 3431-3431 (2017-06-15)
The induction of host cell autophagy by various autophagy inducers contributes to the antimicrobial host defense against Mycobacterium tuberculosis (Mtb), a major pathogenic strain that causes human tuberculosis. In this study, we present a role for the newly identified cyclic
The Use of Inhibitors in Studies on Protein Synthesis.
Pestka, S
Methods in Enzymology, 30, 261-282 (1975)
Kushan L Gunawardhana et al.
Current biology : CB, 27(22), 3442-3453 (2017-11-07)
In Drosophila, the circadian clock is comprised of transcriptional feedback loops that control rhythmic gene expression responsible for daily rhythms in physiology, metabolism, and behavior. The core feedback loop, which employs CLOCK-CYCLE (CLK-CYC) activators and PERIOD-TIMELESS (PER-TIM) repressors to drive
Yuhong Han et al.
STAR protocols, 2(1), 100376-100376 (2021-03-09)
The Fused (Fu) kinase is a key transducer of Hedgehog signaling, but its relevant substrates have remained obscured due to the difficulty of obtaining active Fu for in vitro kinase assay. Based on the mechanism of Fu activation in vivo
Amanda Hopes et al.
Bio-protocol, 7(23), e2625-e2625 (2017-12-05)
Genome editing in diatoms has recently been established for the model species Phaeodactylum tricornutum and Thalassiosira pseudonana. The present protocol, although developed for T. pseudonana, can be modified to edit any diatom genome as we utilize the flexible, modular Golden
D.A.A. Mossel et al.
Archiv Fur Lebensmittelhygiene, 29, 121-121 (1978)
Jin Kyung Kim et al.
Nature communications, 9(1), 4184-4184 (2018-10-12)
Gamma-aminobutyric acid (GABA) is the principal inhibitory neurotransmitter in the brain; however, the roles of GABA in antimicrobial host defenses are largely unknown. Here we demonstrate that GABAergic activation enhances antimicrobial responses against intracellular bacterial infection. Intracellular bacterial infection decreases
Yusheng Zhao et al.
Nature, 583(7818), 825-829 (2020-07-17)
Temperature is a key factor in the growth and development of all organisms1,2. Plants have to interpret temperature fluctuations, over hourly to monthly timescales, to align their growth and development with the seasons. Much is known about how plants respond
Simon A Jackson et al.
Cell host & microbe, 25(2), 250-260 (2019-01-22)
Many prokaryotes possess CRISPR-Cas adaptive immune systems to defend against viruses and invading mobile genetic elements. CRISPR-Cas immunity relies on genetic memories, termed spacers, for sequence-specific recognition of infections. The diversity of spacers within host populations is important for immune
Andreas Puyskens et al.
Cell host & microbe, 27(2), 238-248 (2020-01-07)
Antimicrobial resistance in tuberculosis (TB) is a public health threat of global dimension, worsened by increasing drug resistance. Host-directed therapy (HDT) is an emerging concept currently explored as an adjunct therapeutic strategy for TB. One potential host target is the
Gang Liu et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(6), 7718-7732 (2020-04-16)
Liver inflammation is a common extraintestinal manifestation in inflammatory bowel disease (IBD), yet, the mechanisms driving gut-liver axis inflammation remain poorly understood. IBD leads to a breakdown in the integrity of the intestinal barrier causing an increase in portal and
Alexander von Tesmar et al.
Cell chemical biology, 24(10), 1216-1227 (2017-09-12)
In vitro reconstitution and biochemical analysis of natural product biosynthetic pathways remains a challenging endeavor, especially if megaenzymes of the nonribosomal peptide synthetase (NRPS) type are involved. In theory, all biosynthetic steps may be deciphered using mass spectrometry (MS)-based analyses of
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