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621-37-4
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关键词:'621-37-4'
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Data Sheet - P0157
321-30 (1982).
4. Waymack, P.P., and van Etten, R.L., Isolation and
characterization of a homogeneous isoenzyme of
wheat germ acid phosphatase. Arch. Biochem.
Biophys., 288, 621-33 (1991).
5. Sugiura
Data Sheet - P1146
321-30 (1982).
4. Waymack, P.P., and van Etten, R.L., Isolation and
characterization of a homogeneous isoenzyme of
wheat germ acid phosphatase. Arch. Biochem.
Biophys., 288, 621-33 (1991).
5. Sugiura
Product Information Sheet - O6881
production of superoxide
radical. Biochem. Pharmacol., 37(2), 349-352
(1988).
4. Stryer, L., Biochemistry, 3rd ed., W.H. Freeman
(New York, NY: 1988), pp. 619-621.
5. Yang, J. J., and Finn, W. F., Effect of
DataSheet - P3752
Pol., 50(4),
1245-1256 (2003).
3. Kruzel, M., and Morawiecka, B., Acta Biochim.
Pol., 29(3-4), 321-330 (1982).
4. Waymack, P.P., and van Etten, R.L., Arch.
Biochem. Biophys., 288(2), 621-633 (1991
Product Information Sheet - PSTIRO
Restriction Endonuclease Pst I
From Providencia stuartii
Cat. No. 10 621 625 001 3 000 units (10 U/�l)
Cat. No. 10 621 633 001 10 000 units (10 U/�l)
Cat. No. 10 798 991 001 10 000 units, high
Data Sheet - P3627
305-46
(1989).
4. Waymack, P.P., and van Etten, R.L., Isolation and
characterization of a homogeneous isoenzyme of
wheat germ acid phosphatase. Arch. Biochem.
Biophys., 288, 621-33 (1991).
5. Brouillard
Data Sheet - 91019
. J. and MacDonald J. B., 1960, J. Bacteriol., 80:164.
9. Garrod, 1966, J. Pathol. Bacteriol., 91:621.
10. Lowrence and Traub, 1969, Appl. Microbiol., 17:839.
Precautions and Disclaimer
Product Information Sheet - CS1050
final volume of Reaction Mixture to 50 µl
3. Vortex the tubes briefly and incubate at 37 °C for
1 hour.
4. During the incubation, prepare the cleanup
columns. Mix the Cleanup Resin (Catalog Number
Data Sheet - B3804
L.K., Invest.
Opthalmol. Vis. Sci., 47(2), 605-613 (2006).
6. Li, X. et al., Oncogene, 25(4), 609-621 (2006).
7. Hearty, S. et al., J. Microbiol. Methods, 66(2),
294-312 (2006).
Data Sheet - 89384
8 min at 37 °C in a thermoshaker
2. Add 50 µL of Lysis Buffer C (red cap) and incubate for 8 min at 37°C and 1,400 rpm in the
thermoshaker
3. Centrifuge for 5 min
Data Sheet - 80065
8 min at 37 °C in a thermoshaker
2. Add 50 µL of Lysis Buffer C (red cap) and incubate for 8 min at 37°C and 1,400 rpm in the
thermoshaker
3. Centrifuge for 5 min
Data Sheet - 39851
8 min at 37 °C in a thermoshaker
2. Add 50 µL of Lysis Buffer C (red cap) and incubate for 8 min at 37°C and 1,400 rpm in the
thermoshaker
3. Centrifuge for 5 min
Data Sheet - 77007
8 min at 37 °C in a thermoshaker
2. Add 50 µL of Lysis Buffer C (red cap) and incubate for 8 min at 37°C and 1,400 rpm in the
thermoshaker
3. Centrifuge for 5 min
Data Sheet - 49417
8 min at 37 °C in a thermoshaker
2. Add 50 µL of Lysis Buffer C (red cap) and incubate for 8 min at 37°C and 1,400 rpm in the
thermoshaker
3. Centrifuge for 5 min
Data Sheet - 42875
8 min at 37 °C in a thermoshaker
2. Add 50 µL of Lysis Buffer C (red cap) and incubate for 8 min at 37°C and 1,400 rpm in the
thermoshaker
3. Centrifuge for 5 min
Data Sheet - 49712
8 min at 37 °C in a thermoshaker
2. Add 50 µL of Lysis Buffer C (red cap) and incubate for 8 min at 37°C and 1,400 rpm in the
thermoshaker
3. Centrifuge for 5 min
Data Sheet - 86827
8 min at 37 °C in a thermoshaker
2. Add 50 µL of Lysis Buffer C (red cap) and incubate for 8 min at 37°C and 1,400 rpm in the
thermoshaker
3. Centrifuge for 5 min
Data Sheet - 76545
8 min at 37 °C in a thermoshaker
2. Add 50 µL of Lysis Buffer C (red cap) and incubate for 8 min at 37°C and 1,400 rpm in the
thermoshaker
3. Centrifuge for 5 min
Data Sheet - 75724
8 min at 37 °C in a thermoshaker
2. Add 50 µL of Lysis Buffer C (red cap) and incubate for 8 min at 37°C and 1,400 rpm in the
thermoshaker
3. Centrifuge for 5 min
Data Sheet - 73582
8 min at 37 °C in a thermoshaker
2. Add 50 µL of Lysis Buffer C (red cap) and incubate for 8 min at 37°C and 1,400 rpm in the
thermoshaker
3. Centrifuge for 5 min
Data Sheet - 67289
8 min at 37 °C in a thermoshaker
2. Add 50 µL of Lysis Buffer C (red cap) and incubate for 8 min at 37°C and 1,400 rpm in the
thermoshaker
3. Centrifuge for 5 min
Data Sheet - 68301
21 924 2555
Fax: (+351) 21 924 2610
Russia
SIGMA-ALDRICH RUS, LLC
Tel: +7 (495) 621 6037
Fax: +7 (495) 621 5923
Singapore
SIGMA-ALDRICH PTE. LTD.
Tel: (+65) 6779 1200
Fax: (+65) 6779 1822
Data Sheet - 49699
-
enrichment culture)
3. Incubate 24 h at 37°C
4. Transfer 0.1 mL first pre-enrichment culture to 10 mL fraser-boullion
5. Incubate 24 h at 37 °C
6. transfer a 2 mL
Data Sheet - 56917
and homogenize for 1 minute in a Stomacher (first pre-enrichment
culture)
3. Incubate 4-6 h at 37°C
4. Incubate 44-48 h at 41,5 °C
5. Remove 0,2 mL of sample from the enrichment
Data Sheet - 55661
21 924 2555
Fax: (+351) 21 924 2610
Russia
SIGMA-ALDRICH RUS, LLC
Tel: +7 (495) 621 6037
Fax: +7 (495) 621 5923
Singapore
SIGMA-ALDRICH PTE. LTD.
Tel: (+65) 6779 1200
Fax: (+65) 6779 1822
Data Sheet - 55662
21 924 2555
Fax: (+351) 21 924 2610
Russia
SIGMA-ALDRICH RUS, LLC
Tel: +7 (495) 621 6037
Fax: +7 (495) 621 5923
Singapore
SIGMA-ALDRICH PTE. LTD.
Tel: (+65) 6779 1200
Fax: (+65) 6779 1822
Data Sheet - R7773
340 and 341 and
serines 259 and 499 (activating), serine 43 (prevents
Ras:GTP binding), and serine 621(constitutive, required
for activity).6
Once active, Raf-1 phosphorylates and activates MAP
kinase
Data Sheet - 12838
351) 21 924 2555
Fax: (+351) 21 924 2610
Russia
SIGMA-ALDRICH RUS, LLC
Tel: +7 (495) 621 6037
Fax: +7 (495) 621 5923
Singapore
SIGMA-ALDRICH PTE. LTD.
Tel: (+65) 6779 1200
Fax: (+65) 6779 1822
Data Sheet - 62533
21 924 2555
Fax: (+351) 21 924 2610
Russia
SIGMA-ALDRICH RUS, LLC
Tel: +7 (495) 621 6037
Fax: +7 (495) 621 5923
Singapore
SIGMA-ALDRICH PTE. LTD.
Tel: (+65) 6779 1200
Fax: (+65) 6779 1822
Data Sheet - 19503
21 924 2555
Fax: (+351) 21 924 2610
Russia
SIGMA-ALDRICH RUS, LLC
Tel: +7 (495) 621 6037
Fax: +7 (495) 621 5923
Singapore
SIGMA-ALDRICH PTE. LTD.
Tel: (+65) 6779 1200
Fax: (+65) 6779 1822
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