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6. Soussi, T. et al., Shaping
Triphosphate Set 4 x 250 μl, 4 x 25 μmol, 100 mM 11 969 064 001
4 x 1,250 μl, 4 x 125 μmol, 100 mM 03 622 614 001
Digoxigenin-11-dUTP, alkali-labile 25 nmol, 25 µl, 1 mM 11 573
dist. water to a ratio of 3 ml/g gel and heat
the tube for 7 min at +100°C to melt the gel and denature the
DNA.
• After cooling to +37° C the DNA/agarose mixture can be
Triphosphate Set
4 x 250 μl, 4 x 25 μmol, 100 mM 11 969 064 001
4 x 1,250 μl, 4 x 125 μmol, 100 mM 03 622 614 001
cDNA Synthesis System 1 kit, up to 10 reactions
double dist. water to a ratio of 3 ml/g gel and
heat the tube for 7 min at 100°C to melt the gel and dena-
ture the DNA.
• After cooling to +37°C the DNA/agarose mixture can be
used directly for labeling.
Triphosphate Set 4 x 250 μl, 4 x 25 μmol, 100 mM 11 969 064 001
4 x 1,250 μl, 4 x 125 μmol, 100 mM 03 622 614 001
Dideoxynucleoside Triphosphate Set 4 x 1 μmol, 4 x 100
Triphosphate Set 4 x 250 μl, 4 x 25 μmol, 100 mM 11 969 064 001
4 x 1,250 μl, 4 x 125 μmol, 100 mM 03 622 614 001
Digoxigenin-11-dUTP, alkali-stable 25 nmol, 25 µl, 1 mM 11 093
Triphosphate Set 4 x 250 μl, 4 x 25 μmol, 100 mM 11 969 064 001
4 x 1,250 μl, 4 x 125 μmol, 100 mM 03 622 614 001
Digoxigenin-11-dUTP, alkali-stable 25 nmol, 25 µl, 1 mM 11 093
PCR grade water to a ratio of 3 ml/g gel and heat the
tube for 7 min at 100°C to melt the gel and denature the DNA.
• After cooling to 37°C the DNA/agarose mixture can be used
directly
Triphosphate Set 4 x 250 μl, 4 x 25 μmol, 100 mM 11 969 064 001
4 x 1,250 μl, 4 x 125 μmol, 100 mM 03 622 614 001
Fluorescein-12-dUTP 25 nmol, 25 µl, 1 mM 11 373 242
Triphosphate Set
4 x 250 μl, 4 x 25 μmol, 100 mM 11 969 064 001
4 x 1,250 μl, 4 x 125 μmol, 100 mM 03 622 614 001
Terminal Transferase 8,000 U, 400 U/μl, 20 tailing or
rates in different media.
Amino Acid Analysis - CHO-M-CSF in C8862
*
*Glycine Day 7 = 279%, Day 12 = 622%
Note
differences
between
Figures 3
and 4.
X60708-CHO 8.5x11 4/23/03 4:34 PM
for 10 min. If using oligo (dT) or
gene-specific primer, proceed immediately to step 7.
7. Incubate at 37°C for 60 min. Use the reaction for amplification immediately or store at –20°C.
Amplification