62971

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Ameliorative effect of curcumin against lead acetate-induced hemato-biochemical alterations, hepatotoxicity, and testicular oxidative damage in rats.

Fatma M Abdelhamid et al.

Environmental science and pollution research international, 27(10), 10950-10965 (2020-01-19)

Lead, toxic heavy metal of global concern, induces toxicity in various organs via oxidative stress. Thereby, in this study, the protective role of curcumin against lead acetate-induced toxicity was evaluated. Thirty-two male albino rats were allocated equally into four groups

Method development for quantification of five tear proteins using selected reaction monitoring (SRM) mass spectrometry.

Simin Masoudi et al.

Investigative ophthalmology & visual science, 55(2), 767-775 (2014-01-11)

To establish the use of selected reaction monitoring (SRM) mass spectrometry for quantification of tear proteins. Tear samples were collected on multiple occasions (7-10 days) from healthy subjects with contact lens wear (CL = 3) and without contact lens wear

Crystallographic studies of the activity of hen egg-white lysozyme.

C C Blake et al.

Proceedings of the Royal Society of London. Series B, Biological sciences, 167(1009), 378-388 (1967-04-18)

A yeast display immunoprecipitation method for efficient isolation and characterization of antigens.

Yong Ku Cho et al.

Journal of immunological methods, 341(1-2), 117-126 (2008-12-02)

Yeast antibody display has found a wide variety of applications including antibody affinity maturation, epitope mapping, and library screening. Here we report a yeast display immunoprecipitation (YDIP) technique that employs yeast cells displaying single-chain antibody fragments (scFv) on their surface

Reentrant liquid-liquid phase separation in protein solutions at elevated hydrostatic pressures.

Johannes Möller et al.

Physical review letters, 112(2), 028101-028101 (2014-02-04)

We present results from small-angle x-ray scattering data on the effect of high pressure on the phase behavior of dense lysozyme solutions in the liquid-liquid phase separation region, and characterize the underlying intermolecular protein-protein interactions as a function of temperature

A lysozyme-like protein in Brucella abortus is involved in the early stages of intracellular replication.

Mariela G Del Giudice et al.

Infection and immunity, 81(3), 956-964 (2013-01-16)

Secretion of proteins in Gram-negative bacteria is a high-energy-consuming process that requires translocation across two membranes and a periplasmic space composed of a mesh-like layer, the peptidoglycan. To achieve this, bacteria have evolved complex secretion systems that cross these barriers

The Borrelia afzelii outer membrane protein BAPKO_0422 binds human factor-H and is predicted to form a membrane-spanning β-barrel.

Adam Dyer et al.

Bioscience reports, 35(4), doi:10-doi:10 (2015-07-17)

The deep evolutionary history of the Spirochetes places their branch point early in the evolution of the diderms, before the divergence of the present day Proteobacteria. As a spirochete, the morphology of the Borrelia cell envelope shares characteristics of both

Sequential separation of lysozyme, ovomucin, ovotransferrin, and ovalbumin from egg white.

E D N S Abeyrathne et al.

Poultry science, 93(4), 1001-1009 (2014-04-08)

Ovalbumin, ovotransferrin, ovomucin, and lysozyme are a few of the egg white proteins that can be used as functional components. The objective of this study was to develop a simple, sequential separation method for multiple proteins from egg white. Separated

Inheritance of the lysozyme inhibitor Ivy was an important evolutionary step by Yersinia pestis to avoid the host innate immune response.

Anne Derbise et al.

The Journal of infectious diseases, 207(10), 1535-1543 (2013-02-14)

Yersinia pestis (the plague bacillus) and its ancestor, Yersinia pseudotuberculosis (which causes self-limited bowel disease), encode putative homologues of the periplasmic lysozyme inhibitor Ivy and the membrane-bound lysozyme inhibitor MliC. The involvement of both inhibitors in virulence remains subject to

[Pharmacological correction of nonspecific resistance and production of proinflammatory cytokines during chronic intoxication with organophosphorus compound VX].

P F Zabrodskiĭ et al.

Eksperimental'naia i klinicheskaia farmakologiia, 75(11), 19-21 (2013-01-18)

It is established in experiments on noninbred rats that the use of imunofan (20 mg/kg daily) and polyoxidonium (150 mg/kg daily) for 7 days on the background of chronic intoxication with organophosphorus agent VX (0.01 LD50, single daily treatment for

Molecular basis for the distinct cellular functions of the Lsm1-7 and Lsm2-8 complexes.

Eric J Montemayor et al.

RNA (New York, N.Y.), 26(10), 1400-1413 (2020-06-11)

Eukaryotes possess eight highly conserved Lsm (like Sm) proteins that assemble into circular, heteroheptameric complexes, bind RNA, and direct a diverse range of biological processes. Among the many essential functions of Lsm proteins, the cytoplasmic Lsm1-7 complex initiates mRNA decay

[The salivary factors related to caries and periodontal disease in children and adolescents with diabetes mellitus].

Meng-xing Wang et al.

Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology, 48(9), 545-549 (2013-12-10)

To detect the salivary factors related to caries and periodontal disease and to analyze the risk of caries and periodontal disease in children and adolescents with diabetes mellitus. The study comprised 30 children with diabetic mellitus, aged 7-15 years old

[The approaches to realization of the therapeutic action of gas-air carbonate baths and lysozyme inhalation during their combined application for the treatment of patients presenting with chronic obstructive pulmonary disease].

N S Aĭrapetova et al.

Voprosy kurortologii, fizioterapii, i lechebnoi fizicheskoi kultury, (2)(2), 8-13 (2013-05-31)

The results of this study indicate that the application of gas-air carbonate baths resulted in the reduction of alveolar hypoxia, moderate increase in the patency of central bronchi, marked improvement of pulmonary and systemic cardiohemodynamics, and functional state of cellular

Balance between early life tolerance and sensitization in allergy: dependence on the timing and intensity of prenatal and postnatal allergen exposure of the mother.

Ana Elisa Fusaro et al.

Immunology, 128(1 Suppl), e541-e550 (2009-09-25)

Allergens can be maternally transferred to the fetus or neonate, though it is uncertain how this initial allergen exposure may impact the development of allergy responses. To evaluate the roles of timing and level of maternal allergen exposure in the

Novel layer-by-layer structured nanofibrous mats coated by protein films for dermal regeneration.

Shangjing Xin et al.

Journal of biomedical nanotechnology, 10(5), 803-810 (2014-04-17)

Layer-by-layer coating technique is effective in modifying the surface of nanofibrous mats, but overmuch film-coating makes the mats less porous to hardly suit the condition for tissue engineering. We developed novel nanofibrous mats layer-by-layer coated by silk fibroin and lysozyme

Investigation on the interaction between cyclophosphamide and lysozyme in the presence of three different kind of cyclodextrins: determination of the binding mechanism by spectroscopic and molecular modeling techniques.

Mahdieh Mansouri et al.

Molecules (Basel, Switzerland), 18(1), 789-813 (2013-01-25)

The interactions between cyclophosphamide (CYC) and lysozyme (LYZ) in the presence of different cyclodextrins (CDs) were investigated by UV absorption, fluorescence spectroscopy, circular dichroism (CD), and molecular modeling techniques under imitated physiological conditions. The UV absorption results showed the formation

Mechanical, physico-chemical, and antimicrobial properties of gelatin-based film incorporated with catechin-lysozyme.

Saroat Rawdkuen et al.

Chemistry Central journal, 6(1), 131-131 (2012-11-09)

Microbial activity is a primary cause of deterioration in many foods and is often responsible for reduced quality and safety. Food-borne illnesses associated with E. coli O157:H7, S. aureus, S. enteritidis and L. monocytogenes are a major public health concern

Optimal superpositioning of flexible molecule ensembles.

Vytautas Gapsys et al.

Biophysical journal, 104(1), 196-207 (2013-01-22)

Analysis of the internal dynamics of a biological molecule requires the successful removal of overall translation and rotation. Particularly for flexible or intrinsically disordered peptides, this is a challenging task due to the absence of a well-defined reference structure that

Developments in x-ray crystallographic structure determination of biological macromolecules.

Elspeth F Garman

Science (New York, N.Y.), 343(6175), 1102-1108 (2014-03-08)

The three-dimensional structures of large biomolecules important in the function and mechanistic pathways of all living systems and viruses can be determined by x-ray diffraction from crystals of these molecules and their complexes. This area of crystallography is continually expanding

A universal, high recovery assay for protein quantitation through temperature programmed liquid chromatography (TPLC).

Dennis J Orton et al.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 921-922, 75-80 (2013-02-26)

As an alternative to direct UV absorbance measurements, estimation of total protein concentration is typically conducted through colorimetric reagent assays. However, for protein-limited applications, the proportion of the sample sacrificed to the assay becomes increasingly significant. This work demonstrates a

Effects of Clostridium butyricum on growth performance, immune function, and cecal microflora in broiler chickens challenged with Escherichia coli K88.

L Zhang et al.

Poultry science, 93(1), 46-53 (2014-02-27)

This study was conducted to investigate the effects of Clostridium butyricumon growth performance, immune function, and cecal microflora in broiler chickens challenged with Escherichia coli K88. Three hundred sixty 1-d-old broiler chickens were randomly divided into 4 treatments: negative control

De novo protein crystal structure determination from X-ray free-electron laser data.

Thomas R M Barends et al.

Nature, 505(7482), 244-247 (2013-11-26)

The determination of protein crystal structures is hampered by the need for macroscopic crystals. X-ray free-electron lasers (FELs) provide extremely intense pulses of femtosecond duration, which allow data collection from nanometre- to micrometre-sized crystals in a 'diffraction-before-destruction' approach. So far

The Rcs stress response and accessory envelope proteins are required for de novo generation of cell shape in Escherichia coli.

Dev K Ranjit et al.

Journal of bacteriology, 195(11), 2452-2462 (2013-04-02)

Interactions with immune responses or exposure to certain antibiotics can remove the peptidoglycan wall of many Gram-negative bacteria. Though the spheroplasts thus created usually lyse, some may survive by resynthesizing their walls and shapes. Normally, bacterial morphology is generated by

Molecular cloning, sequence analysis and phylogeny of first caudata g-type lysozyme in axolotl (Ambystoma mexicanum).

Haining Yu et al.

Zoological science, 30(11), 938-943 (2013-11-10)

Lysozymes are key proteins that play important roles in innate immune defense in many animal phyla by breaking down the bacterial cell-walls. In this study, we report the molecular cloning, sequence analysis and phylogeny of the first caudate amphibian g-lysozyme:

Towards absolute quantification of allergenic proteins in food--lysozyme in wine as a model system for metrologically traceable mass spectrometric methods and certified reference materials.

Adam Cryar et al.

Journal of AOAC International, 96(6), 1350-1361 (2014-03-22)

Current routine food allergen quantification methods, which are based on immunochemistry, offer high sensitivity but can suffer from issues of specificity and significant variability of results. MS approaches have been developed, but currently lack metrological traceability. A feasibility study on

Human recombinant lysozyme downregulates advanced glycation endproduct-induced interleukin-6 production and release in an in-vitro model of human proximal tubular epithelial cells.

Davide Gallo et al.

Experimental biology and medicine (Maywood, N.J.), 239(3), 337-346 (2014-02-06)

Diabetic nephropathy is the leading cause of chronic renal disease and one of the major causes of cardiovascular mortality. Evidence suggests that its progression is due to the chronic hyperglycemia consequent to the production and accumulation of advanced glycation endproducts

Different incubation patterns affect selective antimicrobial properties of the egg interior: experimental evidence from eggs of precocial and altricial birds.

Jana Svobodová et al.

The Journal of experimental biology, 222(Pt 6) (2019-03-01)

Avian eggs contend with omnipresent microorganisms entering the egg interior, where they affect embryo viability and hatchling phenotype. The incubation behaviour and deposition of egg white antimicrobial proteins (AMPs) vary highly across the avian altricial-precocial spectrum. Experimental evidence of how

Membrane fouling by the aggregations formed from oppositely charged organic foulants.

Senlin Shao et al.

Water research, 159, 95-101 (2019-05-13)

Due to the lack of robust ways to quantify aggregations, fouling of two-foulant aggregations is poorly understood. This work systematically reports the ultrafiltration membrane fouling by aggregations formed from two oppositely charged organic foulants (i.e., humic acid (HA) and lysozyme

Immunity for nothing and the eggs for free: Apparent lack of both physiological trade-offs and terminal reproductive investment in female crickets (Gryllus texensis).

Atsushi Miyashita et al.

PloS one, 14(5), e0209957-e0209957 (2019-05-16)

Should females alter their reproductive strategy when attacked by pathogens? Two hypotheses provide opposite predictions. Terminal reproductive investment theory predicts that reproduction should increase when the risk of death increases. However, physiological trade-offs between reproduction and immune function might be

One-step production of protein-loaded PLGA microparticles via spray drying using 3-fluid nozzle.

Feng Wan et al.

Pharmaceutical research, 31(8), 1967-1977 (2014-02-20)

The aim of this study was to investigate the potential of using a spray-dryer equipped with a 3-fluid nozzle to microencapsulate protein drugs into polymeric microparticles. Lysozyme and PLGA were used as a model protein and a model polymer, respectively.

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