661376

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Mutagenicity of commercial hair dyes and detection of 2,7-diaminophenazine.

T Watanabe et al.

Mutation research, 244(4), 303-308 (1990-08-01)

Four commercial oxidative-type hair dye formulations, A, B, C, and D, were treated with hydrogen peroxide (H2O2) to simulate normal conditions of use, and the oxidized hair dyes were tested for their mutagenicity in Salmonella typhimurium TA98 in the presence

Spectrophotometric quantification of lactose in solution with a peroxidase-based enzymatic cascade reaction system.

Sara Fornera et al.

Analytical and bioanalytical chemistry, 401(7), 2307-2310 (2011-08-17)

A spectrophotometric assay was developed for the quantification of lactose in aqueous solution via a one-pot enzymatic cascade reaction at 25 °C and pH 7.2. Lactose (0.2-1.8 mM), E. coli β-galactosidase (β-Gal), Aspergillus niger glucose oxidase (GOD), horseradish peroxidase (HRP)

Phenazine-2,3-diamine.

R P Doyle et al.

Acta crystallographica. Section C, Crystal structure communications, 57(Pt 1), 104-105 (2001-02-15)

The planar electron-rich heterocyclic diamine 2,3-diaminophenazine (DAP), C12H10N4, is of particular interest to both chemists and biochemists because of its rich organic chemistry and intense luminescence. In this paper, we report the first structure of DAP in its non-protonated form

2,3-Diaminophenazine is the product from the horseradish peroxidase-catalyzed oxidation of o-phenylenediamine.

P J Tarcha et al.

Analytical biochemistry, 165(1), 230-233 (1987-08-15)

NMR and mass spectroscopic evidence has been obtained which indicates that the product of the oxidation of o-phenylenediamine by hydrogen peroxide, uncatalyzed or catalyzed by horseradish peroxidase, is 2,3-diaminophenazine. These results settle disparate literature descriptions. The process is most likely

Mutagenic analysis of 2,3-diaminophenazine and 2-amino-3-hydroxyphenazine in Salmonella strains expressing different levels of O-acetyltransferase with and without plant and mammalian activation.

E D Wagner et al.

Mutation research, 372(1), 65-74 (1996-11-11)

2,3-Diaminophenazine (DAP) and 2-amino-3-hydroxyphenazine (AHP) are products generated from oxidative-type phenylenediamine hair dyes and are also present in pesticide formulations as contaminants. Earlier studies demonstrated that DAP and AHP were mutagenic in Salmonella typhimurium strains after mammalian microsomal activation. Plant

Evaluation of carbendazim for gene mutations in the Salmonella/Ames plate-incorporation assay: the role of aminophenazine impurities.

A M Sarrif et al.

Mutation research, 321(1-2), 43-56 (1994-04-01)

Benomyl (methyl [1-[(butylamino)carbonyl]-1H-benzimidazol-2- yl]carbamate) and its major metabolite carbendazim (methyl 2-benzimidazolecarbamate) are major agricultural systemic fungicides. These compounds inhibit fungal microtubular function and thereby cause nondisjunction of chromosomes at cell division. Several investigators have proposed that these compounds can also

Chemiluminescent imaging analysis of interferon alpha in serum samples.

Lingyan Hou et al.

Analytical and bioanalytical chemistry, 387(3), 925-931 (2007-01-11)

Enzyme-linked immunosorbent assay (ELISA), horseradish peroxidase (HRP)-catalyzed fluorescent reaction, and oxalate chemiluminescence imaging analysis have been combined to develop a sensitive, simple, and rapid method for analysis of interferon alpha (alpha-IFN) in human serum samples. A typical "sandwich type" immunoassay

Photoinduced DNA cleavage and cellular damage in human dermal fibroblasts by 2,3-diaminophenazine.

Patty K-L Fu et al.

Photochemistry and photobiology, 81(1), 89-95 (2004-10-21)

Aromatic amines, such as o-phenylenediamine (OPD), have been used extensively in commercial hair dyes and in the synthesis of agricultural pesticides. Air oxidation of OPD results in the formation of 2,3-diaminophenazine (DAP). Although the mutagenic toxicity of DAP has been

Identification of 2,3-diaminophenazine and of o-benzoquinone dioxime as the major in vitro metabolites of benzofuroxan.

G Grosa et al.

Xenobiotica; the fate of foreign compounds in biological systems, 34(4), 345-352 (2004-07-23)

1. The results of an in vitro study of the metabolism of benzofuroxan using either cytosolic or microsomal fractions obtained from rat liver are reported. 2. Benzofuroxan was incubated with an appropriate volume of cytosol or microsomal suspension; control incubations

Effects of advanced glycation end-product inhibition and cross-link breakage in diabetic rats.

P S Oturai et al.

Metabolism: clinical and experimental, 49(8), 996-1000 (2000-08-23)

The accelerated formation of advanced glycation end-products (AGEs) due to elevated glycemia has repeatedly been reported as a central pathogenic factor in the development of diabetic microvascular complications. The effects of a novel inhibitor of AGE formation, NNC39-0028 (2,3-diaminophenazine), and

The mutagenic modulating effect of p-phenylenediamine on the oxidation of o- or m-phenylenediamine with hydrogen peroxide in the Salmonella test.

T Watanabe et al.

Mutation research, 245(1), 15-22 (1990-09-01)

The mutagenicity of o- and m-phenylenediamine (PD) was remarkedly enhanced by oxidation; their major mutagenic oxidation products were 2,3- and 2,7-diaminophenazine, respectively. In order to evaluate the modulation effect of p-PD on the oxidation of m- or o-PD, p-PD and

Experimental (FT-IR, FT-Raman and UV-Vis) spectra and theoretical DFT investigations of 2,3-diaminophenazine.

S Sylvestre et al.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 96, 401-412 (2012-06-23)

Vibrational analysis of the planar electron-rich heterocyclic 2,3-diaminophenazine (DAP) molecule was carried out using FT-IR and FT-Raman spectroscopic techniques. The equilibrium geometry, harmonic vibrational wavenumbers, various bonding features have been computed using density functional method. The calculated molecular geometry parameters

Determination of myoglobin based on its enzymatic activity by stopped-flow spectrophotometry.

Qi Zheng et al.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 61(6), 1035-1038 (2005-03-03)

A new method has been developed for the determination of myoglobin (Mb) based on its enzymatic activity for the oxidation of o-phenylenediamine (OPDA) with hydrogen peroxide. Stopped-flow spectrophotometry was used to study the kinetic behavior of the oxidation reaction. The

Identification of intermediate and product from methemoglobin-catalyzed oxidation of o-phenylenediamine in two-phase aqueous-organic system.

De-Jia Li et al.

Biochemistry. Biokhimiia, 70(1), 92-99 (2005-02-11)

Methemoglobin (metHb) was used as a mimetic enzyme for peroxidase to catalyze the oxidation reaction of o-phenylenediamine (OPDA) with H2O2 functioning as an oxidant. A reaction intermediate was obtained in two-phase aqueous-organic system and an absorption peak at 710 nm

Ratiometric Fluorescence Detection of Phosphorylated Amino Acids Through Excited-State Proton Transfer by Using Molecularly Imprinted Polymer (MIP) Recognition Nanolayers.

Wei Wan et al.

Chemistry (Weinheim an der Bergstrasse, Germany), 23(63), 15974-15983 (2017-09-05)

A 2,3-diaminophenazine bis-urea fluorescent probe monomer (1) was developed. It responds to phenylphosphate and phosphorylated amino acids in a ratiometric fashion with enhanced fluorescence accompanied by the development of a redshifted emission band arising from an excited-state proton transfer (ESPT)

Studies on the genotoxic effects of aminophenazines using two cellular models and three different methods.

A Cebulska-Wasilewska et al.

Mutation research, 446(1), 57-65 (1999-12-29)

This paper presents studies on the genotoxicity of two aminophenazines: 2,3-diaminophenazine (DAP) and 2-amino-3-hydroxyphenazine (AHP). The genotoxic activities of these compounds were evaluated with human lymphocytes using the alkaline single cell gel electrophoresis (SCGE) assay and two cytogenetic assays (chromosome

A sensitive resonance scattering spectral assay for the determination of trace H2O2 based on the hrp catalytic reaction and nanogold aggregation.

Aihui Liang et al.

Journal of fluorescence, 18(6), 1035-1041 (2008-02-09)

Gold nanoparticles in size of 15 nm exhibit a resonance scattering (RS) peak at 580 nm, in pH 6.4 citrate buffer solutions. Horseradish peroxidase (HRP) strongly catalyzed the H2O2 oxidation of o-phenylenediamine to form an intergradation of cyclohexa-3, 5-diene-1, 2-diylidenediamine

Electrochemical immunoassay based on catalytic conversion of substrate by labeled metal ion and polarographic detection of the product generated.

W Guo et al.

Analytical biochemistry, 259(1), 74-79 (1998-05-30)

In a new electrochemical immunoassay based on the conversion of a substrate catalyzed by a labeled metal ion and the polarographic detection of the product generated, metal copper ion was used to label model antigen human serum albumin through the

[The study on reaction kinetics based on a new system of the horseradish peroxidase catalyting the oxidation of o-phenylenediamine by H2O2].

Yuan-liang Jiang et al.

Guang pu xue yu guang pu fen xi = Guang pu, 22(3), 436-440 (2003-08-27)

In this paper, we study the enzyme catalysis reaction kinetics that 2,3-diaminophenazine compound was synthesized with the horseradish peroxidase catalyzing reaction of H2O2 oxidizing o-phenylemediamine. First, the product of oxidation of o-phenylenediamine (OPD) by H2O2 catalyzed and by horseradish peroxidase

Spectrophotometric quantification of horseradish peroxidase with o-phenylenediamine.

Sara Fornera et al.

Analytical biochemistry, 407(2), 293-295 (2010-08-10)

The assay conditions for the spectrophotometric quantification of horseradish peroxidase (HRP) with the chromogenic substrate o-phenylenediamine (OPD) at 25°C in the presence of hydrogen peroxide (H(2)O(2)) were optimized. With [OPD](0)=3.14 mM and [H(2)O(2)](0)=80 μM at pH 7.2, the initial formation

A novel inhibitor of advanced glycation end-product formation inhibits mesenteric vascular hypertrophy in experimental diabetes.

T Soulis et al.

Diabetologia, 42(4), 472-479 (1999-05-07)

Previous studies in our laboratory have shown that the vascular changes in diabetes include hypertrophy of the mesenteric vasculature and that this process can be attenuated by the inhibition of advanced glycation with aminoguanidine. Since aminoguanidine can also act as

Development of an enzymatic microreactor based on microencapsulated laccase with off-line capillary electrophoresis for measurement of oxidation reactions.

Georgiana Roman-Gusetu et al.

Journal of chromatography. A, 1216(47), 8270-8276 (2009-09-22)

Microencapsulation is used here as a new technique to immobilize enzymes in a microreactor coupled off-line to capillary electrophoresis (CE), allowing the determination of enzymatic reaction products. The redox enzyme laccase was encapsulated using the method of interfacial cross-linking of

Solid state π-π stacking and higher order dimensional crystal packing, reactivity, and electrochemical behaviour of salphenazine actinide and transition metal complexes.

E E Hardy et al.

Dalton transactions (Cambridge, England : 2003), 45(36), 14243-14251 (2016-08-19)

Condensation of a 2,3-diaminophenazine or 2,3-diamino-2-quinoxalinol with two equivalents of 3,5-ditertbutylsalicylaldehyde affords new Schiff base ligands. Here, we describe and compare the synthesis, UV-Vis, electrochemical, solution, and solid state behaviour of the free base, salphenazine ligand [L(I)], and M[L(I)] complexes

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