Firefly Luciferase. Methods in
Enzymology, 57, 5 (1978).
4. Chappelle, E. W., et al., Determination of Bacterial
Content in Fluids. Methods in Enzymology, 57, 65-
72 (1978).
5. Karl, D. M., Determination
. J., Methods Enzymol., 57, 15-28
(1978).
5. DeLuca, M., and McElroy, W. D., Methods
Enzymol., 57, 3-15 (1978).
6. Chappelle, E. W. et al., Methods Enzymol., 57,
65-72 (1978).
7. Karl, D.
30 times using 92 °C for one minute, 57 °C for
one minute, 72 °C for two minutes. A final five-minute
extension at 72 °C was added at the end.
Results
Figure 1 shows amplification of c-Jun was
and multiple freeze/thaw cycles.
Figure 1.
SDS-PAGE Gel of Typical Lot
70–95% (densitometry)
p 19INK4D43
72
95
130
170
56
34
References
1. Guan, K.L. et al., Isolation and characterization
Chappelle, E.W., et al., Determination of bacterial
content in fluids, Meth. Enzymol., 57, 65-72 (1978).
RBG,MAM 02/09-1
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich, Inc. warrants
References
1. Bernt, E., and Ingeborg, G., Methods of Enzymatic
Analysis, Bergmeyer, H.U., ed., Academic Press
(New York, NY: 1974), pp. 1499-1503.
2. Jornvall, H., Eur. J. Biochem., 72, 425-442
groups. Eur. J. Biochem.,
11(1), 73-79 (1969).
4. Sund, H., and Theorell, H. in The Enzymes, 2nd
ed., Boyer, P. D., ed., Academic Press (New York,
NY: 1963), pp. 57-83.
5. Kagi, J. R. H., and
bacterial
content in fluids. Meth. Enzymol., 57, 65-72 (1978).
GloMax is a registered trademark of Promega Corporation.
DS,GCY,DT,MAM 11/16-1
2016 Sigma-Aldrich Co. LLC. All
concentrate – 1 x 60 mL
Conjugate (antibodies conjugated to peroxidase) – ready
to use – 1 x 16 mL
Substrate (Urea H2O2) – ready‐to‐use – 1 x 57 mL
Chromogen (TMB) – ready‐to‐use – 1 x 57 mL
of bacterial
content in fluids. Meth. Enzymol., 57, 65-72 (1978).
GloMax is a registered trademark of Promega Corporation.
DS,DT,MAM 11/16-1
2016 Sigma-Aldrich Co. LLC. All rights
zur Anreicherung von Salmonellen aus Kotproben
von Tieren. - Berl. Münch. Tierärztl. Wschr., 101; 57-59 (1988).
OSSMER, R.: Salmosyst ® and RAMBACH-Agar. A Rapid Alternative for the Detection of Salmonella
5. Chappelle, E.W., et al., Determination of bacterial
content in fluids, Meth. Enzymol., 57, 65-72 (1978).
DLT/PHC/MAM 4/02
Sigma brand products are sold through Sigma-Aldrich
. Brit J Cancer, 1995, 72:813-7.
4. Soos MA, et al. Biochemical Journal, 1993, 290:419-26.
5. Soos MA, et al. Advances in Experimental Medicine and Biology, 1993,
343:145-57.
Important
solutions:
a. 200 mM solution of 3-NPH·HCl in
ACN:water (1:1) (57 mg in 1.5 ml).
b. 120 mM solution of EDC·HCl in
ACN:water (1:1) (34.5 mg in
1.5 ml). Add 90 µL of Pyridine
(6% final
, J. Biolumin. Chemilumin., 4(1),
375-380 (1989).
3. Kricka, L. J., Anal. Biochem., 175(1), 14-21
(1988).
4. Chappelle, E. W. et al., Meth. Enzymol., 57,
65-72 (1978).
5. Mikolajewicz, N