Jiao-Jiao Shangguan et al.
Applied biochemistry and biotechnology, 168(7), 1820-1833 (2012-10-12)
A 1,080-bp cDNA (CGMCC 2873) encoding of a cold-active lipase of Aspergillus fumigatus (AFL67) was cloned and expressed in Escherichia coli for the first time. The new lipase, AFL67, was one-step purified by 8.30 folds through Ni-NTA affinity chromatography with