840032P

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Periprotein lipidomes of Saccharomyces cerevisiae provide a flexible environment for conformational changes of membrane proteins.

Joury S van 't Klooster et al.

eLife, 9 (2020-04-18)

Yeast tolerates a low pH and high solvent concentrations. The permeability of the plasma membrane (PM) for small molecules is low and lateral diffusion of proteins is slow. These findings suggest a high degree of lipid order, which raises the

Interaction of Myelin Basic Protein with Myelin-like Lipid Monolayers at Air-Water Interface.

Katharina Widder et al.

Langmuir : the ACS journal of surfaces and colloids, 34(21), 6095-6108 (2018-05-04)

Interaction of myelin basic protein (MBP) and the cytoplasmic leaflets of the oligodendrocyte membrane is essential for the formation and compaction of the myelin sheath of the central nervous system and is altered aberrantly and implicated in the pathogenesis of

How synaptotagmin promotes membrane fusion.

Sascha Martens et al.

Science (New York, N.Y.), 316(5828), 1205-1208 (2007-05-05)

Synaptic vesicles loaded with neurotransmitters are exocytosed in a soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE)-dependent manner after presynaptic depolarization induces calcium ion (Ca2+) influx. The Ca2+ sensor required for fast fusion is synaptotagmin-1. The activation energy of bilayer-bilayer fusion

Isolation of phosphatidylethanolamine as a solitary cofactor for prion formation in the absence of nucleic acids.

Nathan R Deleault et al.

Proceedings of the National Academy of Sciences of the United States of America, 109(22), 8546-8551 (2012-05-16)

Infectious prions containing the pathogenic conformer of the mammalian prion protein (PrP(Sc)) can be produced de novo from a mixture of the normal conformer (PrP(C)) with RNA and lipid molecules. Recent reconstitution studies indicate that nucleic acids are not required

Kidney injury molecule-1 is a phosphatidylserine receptor that confers a phagocytic phenotype on epithelial cells.

Takaharu Ichimura et al.

The Journal of clinical investigation, 118(5), 1657-1668 (2008-04-17)

Following injury, the clearance of apoptotic and necrotic cells is necessary for mitigation and resolution of inflammation and tissue repair. In addition to macrophages, which are traditionally assigned to this task, neighboring epithelial cells in the affected tissue are postulated

LipidFrag: Improving reliability of in silico fragmentation of lipids and application to the Caenorhabditis elegans lipidome

Witting M, et al.

PLoS ONE, 12(3), e0172311-e0172311 (2017)

Qualitative and quantitative characterization of protein-phosphoinositide interactions with liposome-based methods.

Ricarda A Busse et al.

Autophagy, 9(5), 770-777 (2013-03-01)

We characterized phosphoinositide binding of the S. cerevisiae PROPPIN Hsv2 qualitatively with density flotation assays and quantitatively through isothermal titration calorimetry (ITC) measurements using liposomes. We discuss the design of these experiments and show with liposome flotation assays that Hsv2

Advantages of the phosphatidylserine-recognizing peptide PSP1 for molecular imaging of tumor apoptosis compared with annexin V.

Soyoun Kim et al.

PloS one, 10(3), e0121171-e0121171 (2015-03-25)

A number of peptide-based indicators have been identified and reported as potential apoptosis probes, offering great promise for early assessment of therapeutic efficacy in several types of cancer. Direct comparison of the newly developed probes with previously used ones would

Using supported bilayers to study the spatiotemporal organization of membrane-bound proteins.

Phuong A Nguyen et al.

Methods in cell biology, 128, 223-241 (2015-05-23)

Cell division in prokaryotes and eukaryotes is commonly initiated by the well-controlled binding of proteins to the cytoplasmic side of the cell membrane. However, a precise characterization of the spatiotemporal dynamics of membrane-bound proteins is often difficult to achieve in

A novel flow cytometric assay to quantify interactions between proteins and membrane lipids.

Koen Temmerman et al.

Journal of lipid research, 50(6), 1245-1254 (2009-01-16)

A diverse set of experimental systems has been developed to probe protein-lipid interactions. These include measurements with the headgroups of membrane lipids in solution, immobilized membrane lipids, and analysis of protein binding to membrane lipids reconstituted in liposomes. Each of

Human autoreactive T cells recognize CD1b and phospholipids.

Ildiko Van Rhijn et al.

Proceedings of the National Academy of Sciences of the United States of America, 113(2), 380-385 (2015-12-02)

In contrast with the common detection of T cells that recognize MHC, CD1a, CD1c, or CD1d proteins, CD1b autoreactive T cells have been difficult to isolate in humans. Here we report the development of polyvalent complexes of CD1b proteins and

Bacterial intracellularly active toxins: Membrane localisation of the active domain.

Carolina Varela-Chavez et al.

Cellular microbiology, 22(7), e13213-e13213 (2020-05-01)

Numerous bacterial toxins exert their activity by inactivating or modulating a specific intracellular host target. For this purpose, these toxins have developed efficient strategies to overcome the different host cell defences including specific binding to cell surface, internalisation, passage through

Analytical characterization of the role of phospholipids in platelet adhesion and secretion.

Secil Koseoglu et al.

Analytical chemistry, 87(1), 413-421 (2014-12-03)

The cellular phospholipid membrane plays an important role in cell function and cell-cell communication, but its biocomplexity and dynamic nature presents a challenge for examining cellular uptake of phospholipids and the resultant effects on cell function. Platelets, small anuclear circulating

Metabolomics and lipidomics using traveling-wave ion mobility mass spectrometry.

Giuseppe Paglia et al.

Nature protocols, 12(4), 797-813 (2017-03-17)

Metabolomics and lipidomics aim to profile the wide range of metabolites and lipids that are present in biological samples. Recently, ion mobility spectrometry (IMS) has been used to support metabolomics and lipidomics applications to facilitate the separation and the identification

Role of phosphatidylinositol(4,5)bisphosphate organization in membrane transport by the Unc104 kinesin motor.

Dieter R Klopfenstein et al.

Cell, 109(3), 347-358 (2002-05-23)

Unc104 (KIF1A) kinesin transports membrane vesicles along microtubules in lower and higher eukaryotes. Using an in vitro motility assay, we show that Unc104 uses a lipid binding pleckstrin homology (PH) domain to dock onto membrane cargo. Through its PH domain

Unique surface-exposed hydrophobic residues in the C1 domain of factor VIII contribute to cofactor function and von Willebrand factor binding.

Małgorzata A Przeradzka et al.

Journal of thrombosis and haemostasis : JTH, 18(2), 364-372 (2019-11-02)

The identity of the amino acid regions of factor VIII (FVIII) that contribute to factor IXa (FIXa) and von Willebrand factor (VWF) binding has not been fully resolved. Previously, we observed that replacing the FVIII C1 domain for the one

Flippase-mediated phospholipid asymmetry promotes fast Cdc42 recycling in dynamic maintenance of cell polarity.

Arupratan Das et al.

Nature cell biology, 14(3), 304-310 (2012-02-22)

Lipid asymmetry at the plasma membrane is essential for such processes as cell polarity, cytokinesis and phagocytosis. Here we find that a lipid flippase complex, composed of Lem3, Dnf1 or Dnf2, has a role in the dynamic recycling of the

A single herpesvirus protein can mediate vesicle formation in the nuclear envelope.

Michael Lorenz et al.

The Journal of biological chemistry, 290(11), 6962-6974 (2015-01-22)

Herpesviruses assemble capsids in the nucleus and egress by unconventional vesicle-mediated trafficking through the nuclear envelope. Capsids bud at the inner nuclear membrane into the nuclear envelope lumen. The resulting intralumenal vesicles fuse with the outer nuclear membrane, delivering the

Membrane permeabilization by trypanosome lytic factor, a cytolytic human high density lipoprotein.

John M Harrington et al.

The Journal of biological chemistry, 284(20), 13505-13512 (2009-03-28)

Trypanosome lytic factor (TLF) is a subclass of human high density lipoprotein (HDL) that mediates an innate immune killing of certain mammalian trypanosomes, most notably Trypanosoma brucei brucei, the causative agent of a wasting disease in cattle. Mechanistically, killing is

Alzheimer's disease-associated TREM2 variants exhibit either decreased or increased ligand-dependent activation

Song W, et al.

Alzheimers Dement., 13(4), 381-387 (2017)

The Tip of the Four N-Terminal α-Helices of Clostridium sordellii Lethal Toxin Contains the Interaction Site with Membrane Phosphatidylserine Facilitating Small GTPases Glucosylation.

Carolina Varela Chavez et al.

Toxins, 8(4), 90-90 (2016-03-31)

Clostridium sordellii lethal toxin (TcsL) is a powerful virulence factor responsible for severe toxic shock in man and animals. TcsL belongs to the large clostridial glucosylating toxin (LCGT) family which inactivates small GTPases by glucosylation with uridine-diphosphate (UDP)-glucose as a

Bovine brain phosphatidylserine attenuates scopolamine induced amnesia in mice

Claro FT, et al.

Progress in Neuro-Psychopharmacology & Biological Psychiatry, 30(5), 881-886 (2006)

Cytoskeletal protein binding kinetics at planar phospholipid membranes.

A E Mc Kiernan et al.

Biophysical journal, 73(4), 1987-1998 (1997-10-23)

It has been hypothesized that nonspecific reversible binding of cytoskeletal proteins to lipids in cells may guide their binding to integral membrane anchor proteins. In a model system, we measured desorption rates k(off) (off-rates) of the erythrocyte cytoskeletal proteins spectrin

Rottlerin is a mitochondrial uncoupler that decreases cellular ATP levels and indirectly blocks protein kinase Cdelta tyrosine phosphorylation.

S P Soltoff

The Journal of biological chemistry, 276(41), 37986-37992 (2001-08-11)

Protein kinase Cdelta (PKCdelta) is activated by stimuli that increase its tyrosine phosphorylation, including neurotransmitters that initiate fluid secretion in salivary gland (parotid) epithelial cells. Rottlerin, a compound reported to be a PKCdelta-selective inhibitor, rapidly increased the rate of oxygen

Mobilization of the A-kinase N-myristate through an isoform-specific intermolecular switch.

M Gangal et al.

Proceedings of the National Academy of Sciences of the United States of America, 96(22), 12394-12399 (1999-10-27)

Although the catalytic (C) subunit of cAMP-dependent protein kinase is N-myristylated, it is a soluble protein, and no physiological role has been identified for its myristyl moiety. To determine whether the interaction of the two regulatory (R) subunit isoforms (R(I)

Effect of Cholesterol and Myelin Basic Protein (MBP) Content on Lipid Monolayers Mimicking the Cytoplasmic Membrane of Myelin.

Jennica Träger et al.

Cells, 9(3) (2020-02-29)

Myelin basic protein (MBP) is located in the insulating covers of nerve cells in the brain and spinal cord. By interacting with lipid membranes, it is responsible for compaction of the myelin sheath in the central nervous system, which is

CD1a-autoreactive T cells recognize natural skin oils that function as headless antigens.

Annemieke de Jong et al.

Nature immunology, 15(2), 177-185 (2013-12-24)

T cells autoreactive to the antigen-presenting molecule CD1a are common in human blood and skin, but the search for natural autoantigens has been confounded by background T cell responses to CD1 proteins and self lipids. After capturing CD1a-lipid complexes, we

Defining How Oncogenic and Developmental Mutations of PIK3R1 Alter the Regulation of Class IA Phosphoinositide 3-Kinases.

Gillian L Dornan et al.

Structure (London, England : 1993), 28(2), 145-156 (2019-12-14)

The class I phosphoinositide 3-kinases (PI3Ks) are key signaling enzymes composed of a heterodimer of a p110 catalytic subunit and a p85 regulatory subunit, with PI3K mutations being causative of multiple human diseases including cancer, primary immunodeficiencies, and developmental disorders.

Structure and flexibility of the endosomal Vps34 complex reveals the basis of its function on membranes.

Ksenia Rostislavleva et al.

Science (New York, N.Y.), 350(6257), aac7365-aac7365 (2015-10-10)

Phosphatidylinositol 3-kinase Vps34 complexes regulate intracellular membrane trafficking in endocytic sorting, cytokinesis, and autophagy. We present the 4.4 angstrom crystal structure of the 385-kilodalton endosomal complex II (PIK3C3-CII), consisting of Vps34, Vps15 (p150), Vps30/Atg6 (Beclin 1), and Vps38 (UVRAG). The

Characterization of brain phosphatidylserine decarboxylase: localization in the mitochondrial inner membrane

Percy AK, et al.

Archives of Biochemistry and Biophysics, 223(2), 484-494 (1983)

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