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Tjeerd Pols et al.
The FEBS journal, 288(1), 293-309 (2020-04-20)
In cells, the breakdown of arginine to ornithine and ammonium ion plus carbon dioxide is coupled to the generation of metabolic energy in the form of ATP. The arginine breakdown pathway is minimally composed of arginine deiminase, ornithine transcarbamoylase, carbamate
Dariusz T Mlynarczyk et al.
Bioorganic chemistry, 96, 103634-103634 (2020-02-12)
An important subgroup within the porphyrazine (Pz) family constitutes seco-porphyrazines, in the chemical structure of which one pyrrole unit is opened in the oxidative process. So far, there are only limited data on N-seco- and C-seco-Pzs. Here, the synthesis of
Benjamin J Wylie et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(1), 185-190 (2013-12-18)
It has been hypothesized that transmembrane allostery is the basis for inactivation of the potassium channel KcsA: opening the intracellular gate is spontaneously followed by ion expulsion at the extracellular selectivity filter. This suggests a corollary: following ion expulsion at
Manasi P Bhate et al.
Protein expression and purification, 91(2), 119-124 (2013-08-07)
We report the expression, purification, liposome reconstitution and functional validation of uniformly (13)C and (15)N isotope labeled KcsA, a bacterial potassium channel that has high homology with mammalian channels, for solid-state NMR studies. The expression and purification is optimized for
Ming-Tao Lee et al.
Biochemistry, 57(38), 5629-5639 (2018-08-29)
Daptomycin is a phosphatidylglycerol specific, calcium-dependent membrane-active antibiotic that has been approved for the treatment of Gram-positive infections. A recent Bacillus subtilis study found that daptomycin clustered into fluid lipid domains of bacterial membranes and the membrane binding was correlated
Juliana Kovalczuk de Oliveira et al.
Materials science & engineering. C, Materials for biological applications, 110, 110720-110720 (2020-03-25)
The use of nanocarriers for drug delivery is a strategy aimed to improve therapeutic indices through changes in their pharmacokinetic and pharmacodynamic characteristics. Liposomes are well-investigated nanocarriers for drug delivery to macrophage-targeted therapy, the main hosts of intracellular pathogens of
Jiajie Diao et al.
Nature protocols, 7(5), 921-934 (2012-05-15)
SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins are a highly regulated class of membrane proteins that drive the efficient merger of two distinct lipid bilayers into one interconnected structure. This protocol describes our fluorescence resonance energy transfer (FRET)-based single
Amit Kumar Khan et al.
Polymers, 12(4) (2020-04-26)
In this work, we have used low-molecular-weight (PEG12-b-PCL6, PEG12-b-PCL9 or PEG16-b-PLA38; MW, 1.25-3.45 kDa) biodegradable block co-polymers to construct nano- and micron-scaled hybrid (polymer/lipid) vesicles, by solvent dispersion and electroformation methods, respectively. The hybrid vesicles exhibit physical properties (size, bilayer
Jing Xu et al.
Molecular pharmaceutics, 10(9), 3366-3374 (2013-08-09)
Herein we report the development of a nonviral lipid-complexed PRINT (particle replication in nonwetting templates) protein particle system (LPP particle) for RNA replicon delivery with a view toward RNA replicon-based vaccination. Cylindrical bovine serum albumin (BSA) particles (diameter (d) 1
Jinhong Meng et al.
Scientific reports, 10(1), 1046-1046 (2020-01-25)
P53 mutations are responsible for drug-resistance of tumour cells which impacts on the efficacy of treatment. Alternative tumour suppressor pathways need to be explored to treat p53- deficient tumours. The E3 ubiquitin ligase, ITCH, negatively regulates the tumour suppressor protein
Gautam K Samdani et al.
Journal of agricultural and food chemistry, 66(15), 3939-3948 (2018-04-10)
Phospholipids have been shown to act synergistically with tocopherols and delay lipid oxidation in bulk oil. The synergistic activity between phospholipids and tocopherols is due to the ability of amino-group-containing phospholipids (e.g., phosphatidylethanolamine (PE) and phosphatidylserine (PS)) to convert oxidized
Jorge Larios et al.
The Journal of cell biology, 219(3) (2020-02-13)
The intraluminal vesicles (ILVs) of endosomes mediate the delivery of activated signaling receptors and other proteins to lysosomes for degradation, but they also modulate intercellular communication when secreted as exosomes. The formation of ILVs requires four complexes, ESCRT-0, -I, -II
The role of the helper lipid on the DNA transfection efficiency of lipopolyplex formulations
Du Z, et al.
Scientific Reports, 4(2), 7107-7107 (2014)
Yasunori Watanabe et al.
Structure (London, England : 1993), 28(7), 799-809 (2020-05-14)
In both prokaryotes and eukaryotes, phosphatidylethanolamine (PE), one of the most abundant membrane phospholipids, plays important roles in various membrane functions and is synthesized through the decarboxylation of phosphatidylserine (PS) by PS decarboxylases (PSDs). However, the catalysis and substrate recognition
In vitro skin penetration enhancement techniques: A combined approach of ethosomes and microneedles.
C Bellefroid et al.
International journal of pharmaceutics, 572, 118793-118793 (2019-11-13)
Dermal administration of different macromolecules, such as nucleic acids, remains a real challenge because of the difficulty of crossing the main skin barrier, the stratum corneum (SC). To overcome this barrier, the use of deformable lipid-based nanovectors were developed to
Jamie B Strachan et al.
Journal of colloid and interface science, 576, 241-251 (2020-05-20)
Cubosomes form part of the next generation of lipid nanoparticle drug delivery vehicles, enabling higher drug encapsulation efficiency, particularly for lipophilic drugs, compared to traditional liposome formulations. However, the mechanism of interaction of cubosome lipid nanoparticles with cells and their
Margaret M Billingsley et al.
Nano letters (2020-01-18)
Chimeric antigen receptor (CAR) T cell therapy relies on the ex vivo manipulation of patient T cells to create potent, cancer-targeting therapies, shown to be capable of inducing remission in patients with acute lymphoblastic leukemia and large B cell lymphoma.
Hye-Ra Lee et al.
International journal of molecular sciences, 21(3) (2020-02-06)
Hepatitis C virus (HCV) p7 is known to be a nonselective cation channel for HCV maturation. Because the interaction of HCV proteins with host lipids in the endoplasmic reticulum membrane is crucial for the budding process, the identification of p7-lipid
Membrane reshaping by micrometric curvature sensitive septin filaments
Beber A, et al.
Nature Communications, 10(1), 420-420 (2019)
Jie He et al.
Antimicrobial agents and chemotherapy, 64(8) (2020-05-20)
Candida albicans is a commensal organism that causes life-threatening or life-altering opportunistic infections. Treatment of Candida infections is limited by the paucity of antifungal drug classes. Naturally occurring antimicrobial peptides are promising agents for drug development. CCL28 is a CC
Michela Antonelli et al.
Food chemistry, 310, 125860-125860 (2019-11-19)
The determination of phospholipids in olive oil is challenging due to their low concentration. For this reason, a comparison of two solid phase extraction procedures, namely weak anionic exchange (WAX) and graphitized carbon black (GCB), is presented for the enrichment
Rashik Ahmed et al.
Journal of the American Chemical Society, 142(21), 9686-9699 (2020-05-10)
Alpha synuclein (αS) oligomers are a key component of Lewy bodies implicated in Parkinson's disease (PD). Although primarily intracellular, extracellular αS exocytosed from neurons also contributes to PD pathogenesis through a prion-like transmission mechanism. Here, we show at progressive degrees
Daniel N Mitroi et al.
Autophagy, 13(5), 885-899 (2017-05-20)
Macroautophagy/autophagy defects have been identified as critical factors underlying the pathogenesis of neurodegenerative diseases. The roles of the bioactive signaling lipid sphingosine-1-phosphate (S1P) and its catabolic enzyme SGPL1/SPL (sphingosine phosphate lyase 1) in autophagy are increasingly recognized. Here we provide
Tatiana K Rostovtseva et al.
The Journal of biological chemistry, 287(35), 29589-29598 (2012-07-06)
Elucidating molecular mechanisms by which lipids regulate protein function within biological membranes is critical for understanding the many cellular processes. Recently, we have found that dimeric αβ-tubulin, a subunit of microtubules, regulates mitochondrial respiration by blocking the voltage-dependent anion channel
Megan B Miller et al.
The Journal of biological chemistry, 290(21), 13541-13555 (2015-04-12)
Previous studies revealed an essential role for the lipid-binding Sec14 domain of kalirin (KalSec14), but its mechanism of action is not well understood. Because alternative promoter usage appends unique N-terminal peptides to the KalSec14 domain, we used biophysical, biochemical, and
Bruno C Borro et al.
Biochimica et biophysica acta. General subjects, 129485-129485 (2019-11-18)
Microgels offer opportunities for improved delivery of antimicrobial peptides (AMP). To contribute to a foundation for rational design of such systems, we here study the effects of electrostatics on the generation of peptide-carrying microgels. For this, alginate microgels loaded with
Éder Dos Santos Souza et al.
Analytical and bioanalytical chemistry, 412(6), 1431-1439 (2020-01-09)
Triatominae are hematophagous insects involved in the transmission of Chagas disease. Among the 19 genera of the subfamily, those with the highest epidemiological importance regarding the dissemination of Trypanosoma cruzi are Panstrongylus, Rhodnius, and Triatoma. Of these three genera, Rhodnius
Tuo-Xian Tang et al.
Biochimica et biophysica acta. Biomembranes, 1862(6), 183230-183230 (2020-03-04)
Changes in membrane curvature are required to control the function of subcellular compartments; malfunctions of such processes are associated with a wide range of human diseases. Membrane remodeling often depends upon the presence of phosphoinositides, which recruit protein effectors for
The role of the helper lipid dioleoylphosphatidylethanolamine (DOPE) for DNA transfection cooperating with a cationic lipid bearing ethylenediamine
Mochizuki S, et al.
Biochimica et Biophysica Acta - Biomembranes, 1828(2), 412-418 (2013)
Philipp M G Löffler et al.
Methods in molecular biology (Clifton, N.J.), 2105, 75-96 (2020-02-24)
Peptide nucleic acids (PNAs) can be modified with aliphatic lipid chains and designed to be water soluble and able to spontaneously insert into phospholipid bilayers. Liposomes with 1.5% negatively charged POPG can be driven to fuse and mix their inner
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