850725P

应用筛选条件

关键词:'850725P'

显示 31-60 共 103 条结果关于 "850725P" 范围论文

Combination therapy with liposomal doxorubicin and liposomal vaccine containing E75, an HER-2/neu-derived peptide, reduces myeloid-derived suppressor cells and improved tumor therapy.

Parvin Zamani et al.

Life sciences, 252, 117646-117646 (2020-04-10)

Myeloid-derived suppressor cells (MDSCs) are immunosuppressive cells causing resistance to immunotherapies in cancer tumors. In the current study, various immunogenic and therapeutic features of the combination therapies with non-liposomal Doxorubicin (Dox) and the E75 immunogenic peptide (Pep), derived from the

Human Papillomavirus Oncogene Manipulation Using Clustered Regularly Interspersed Short Palindromic Repeats/Cas9 Delivered by pH-Sensitive Cationic Liposomes.

Shuai Zhen et al.

Human gene therapy (2020-01-25)

Clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) technology enables targeted gene editing, but cancer gene therapy with this approach requires improvements to enable safe and efficient delivery of CRISPR/Cas9 to tumors. We developed and evaluated a self-assembled

On the role of helper lipids in lipid nanoparticle formulations of siRNA.

Jayesh A Kulkarni et al.

Nanoscale, 11(45), 21733-21739 (2019-11-13)

Onpattro, the first RNAi-based therapeutic to receive FDA approval, is enabled by a lipid nanoparticle (LNP) system that facilitates siRNA delivery into the cytoplasm of target cells (hepatocytes) following intravenous (i.v.) administration. These LNP-siRNA systems consist of four lipid components

Endoplasmic reticulum-targeted glutathione and pH dual responsive vitamin lipid nanovesicles for tocopheryl DM1 delivery and cancer therapy.

Yu-Qing Wang et al.

International journal of pharmaceutics, 582, 119331-119331 (2020-04-15)

The major drawbacks of the cytotoxin like DM1 are the off-target effects. Here, the targeting nanovesicles were developed by synthesizing tocopherol-SS-DM1 and conjugating a pH low insertion peptide (pHLIP) to PEGylated phospholipids, in which tocopherol-SS-DM1 improves the drug loading and

A single vesicle-vesicle fusion assay for in vitro studies of SNAREs and accessory proteins.

Jiajie Diao et al.

Nature protocols, 7(5), 921-934 (2012-05-15)

SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins are a highly regulated class of membrane proteins that drive the efficient merger of two distinct lipid bilayers into one interconnected structure. This protocol describes our fluorescence resonance energy transfer (FRET)-based single

RNA replicon delivery via lipid-complexed PRINT protein particles.

Jing Xu et al.

Molecular pharmaceutics, 10(9), 3366-3374 (2013-08-09)

Herein we report the development of a nonviral lipid-complexed PRINT (particle replication in nonwetting templates) protein particle system (LPP particle) for RNA replicon delivery with a view toward RNA replicon-based vaccination. Cylindrical bovine serum albumin (BSA) particles (diameter (d) 1

ALIX- and ESCRT-III-dependent sorting of tetraspanins to exosomes.

Jorge Larios et al.

The Journal of cell biology, 219(3) (2020-02-13)

The intraluminal vesicles (ILVs) of endosomes mediate the delivery of activated signaling receptors and other proteins to lysosomes for degradation, but they also modulate intercellular communication when secreted as exosomes. The formation of ILVs requires four complexes, ESCRT-0, -I, -II

Silencing E3 Ubiqutin ligase ITCH as a potential therapy to enhance chemotherapy efficacy in p53 mutant neuroblastoma cells.

Jinhong Meng et al.

Scientific reports, 10(1), 1046-1046 (2020-01-25)

P53 mutations are responsible for drug-resistance of tumour cells which impacts on the efficacy of treatment. Alternative tumour suppressor pathways need to be explored to treat p53- deficient tumours. The E3 ubiquitin ligase, ITCH, negatively regulates the tumour suppressor protein

Molecular Mechanisms Responsible for Pharmacological Effects of Genipin on Mitochondrial Proteins.

Jürgen Kreiter et al.

Biophysical journal, 117(10), 1845-1857 (2019-11-11)

Genipin, a natural compound from Gardenia jasminoides, is a well-known compound in Chinese medicine that is used for the treatment of cancer, inflammation, and diabetes. The use of genipin in classical medicine is hindered because of its unknown molecular mechanisms

Facile Mixing of Phospholipids Promotes Self-Assembly of Low-Molecular-Weight Biodegradable Block Co-Polymers into Functional Vesicular Architectures.

Amit Kumar Khan et al.

Polymers, 12(4) (2020-04-26)

In this work, we have used low-molecular-weight (PEG12-b-PCL6, PEG12-b-PCL9 or PEG16-b-PLA38; MW, 1.25-3.45 kDa) biodegradable block co-polymers to construct nano- and micron-scaled hybrid (polymer/lipid) vesicles, by solvent dispersion and electroformation methods, respectively. The hybrid vesicles exhibit physical properties (size, bilayer

Contact-Triggered Lipofection from Multilayer Films Designed as Surfaces for in Situ Transfection Strategies in Tissue Engineering.

Catharina Husteden et al.

ACS applied materials & interfaces, 12(8), 8963-8977 (2020-02-01)

Biomaterials, which release active compounds after implantation, are an essential tool for targeted regenerative medicine. In this study, thin multilayer films loaded with lipid/DNA complexes (lipoplexes) were designed as surface coatings for in situ transfection applicable in tissue engineering and

Enzymology of the pathway for ATP production by arginine breakdown.

Tjeerd Pols et al.

The FEBS journal, 288(1), 293-309 (2020-04-20)

In cells, the breakdown of arginine to ornithine and ammonium ion plus carbon dioxide is coupled to the generation of metabolic energy in the form of ATP. The arginine breakdown pathway is minimally composed of arginine deiminase, ornithine transcarbamoylase, carbamate

Development And In Vitro Characterization Of Bladder Tumor Cell Targeted Lipid-Coated Polyplex For Dual Delivery Of Plasmids And Small Molecules.

Shayak Samaddar et al.

International journal of nanomedicine, 14, 9547-9561 (2019-12-12)

Bladder cancer is the fourth most common cancer in men and eleventh most common in women. Combination therapy using a gene and chemotherapeutic drug is a potentially useful strategy for treating bladder cancer in cases where a synergistic benefit can

A triple chain polycationic peptide-mimicking amphiphile - efficient DNA-transfer without co-lipids.

Shashank Reddy Pinnapireddy et al.

Biomaterials science, 8(1), 232-249 (2019-11-05)

Non-viral gene delivery in its current form is largely dependent upon the ability of a delivery vehicle to protect its cargo in the extracellular environment and release it efficiently inside the target cell. Also a simple delivery system is required

Interactions of Cationic Lipids with DNA: A Structural Approach.

Matthias Dittrich et al.

Langmuir : the ACS journal of surfaces and colloids (2018-09-01)

Colloidal nucleic acid carrier systems based on cationic lipids are a promising pharmaceutical tool in the implementation of gene therapeutic strategies. This study demonstrates the complex behavior of DNA at the lipid-solvent interface facilitating structural changes of the lyotropic liquid-crystalline

De novo synthesized Min proteins drive oscillatory liposome deformation and regulate FtsA-FtsZ cytoskeletal patterns.

Elisa Godino et al.

Nature communications, 10(1), 4969-4969 (2019-11-02)

The Min biochemical network regulates bacterial cell division and is a prototypical example of self-organizing molecular systems. Cell-free assays relying on purified proteins have shown that MinE and MinD self-organize into surface waves and oscillatory patterns. In the context of

Covalently linked dengue virus envelope glycoprotein dimers reduce exposure of the immunodominant fusion loop epitope.

Alexander Rouvinski et al.

Nature communications, 8, 15411-15411 (2017-05-24)

A problem in the search for an efficient vaccine against dengue virus is the immunodominance of the fusion loop epitope (FLE), a segment of the envelope protein E that is buried at the interface of the E dimers coating mature

Liposomal delivery of functional transmembrane ion channels into the cell membranes of target cells; a potential approach for the treatment of channelopathies.

Shahenda Ramadan et al.

International journal of biological macromolecules, 153, 1080-1089 (2019-11-23)

Defects in transmembrane ion channels underlie many disorders, commonly known as channelopathies. Current therapies are mostly symptomatic and do not treat the underlying cause. Here, we demonstrate the delivery of functional ion channels in protein form into the membrane of

Not only oxidation of cardiolipin affects the affinity of cytochrome C for lipid bilayers.

Cintia Kawai et al.

The journal of physical chemistry. B, 118(41), 11863-11872 (2014-09-24)

Fluorescence quenching of lipid-bound pyrene was used to assess the binding of cytochrome c (cyt c) to liposomes that mimic the inner mitochondrial membrane (IMM) POPC/DOPE/TOCL, with the conditions that it did or did not contain oxidized phosphatidylcholine molecules, i.e.

Validation of a mitochondrial RNA therapeutic strategy using fibroblasts from a Leigh syndrome patient with a mutation in the mitochondrial ND3 gene.

Yuma Yamada et al.

Scientific reports, 10(1), 7511-7511 (2020-05-07)

We report on the validation of a mitochondrial gene therapeutic strategy using fibroblasts from a Leigh syndrome patient by the mitochondrial delivery of therapeutic mRNA. The treatment involves delivering normal ND3 protein-encoding mRNA as a therapeutic RNA to mitochondria of

Using microfluidics for scalable manufacturing of nanomedicines from bench to GMP: A case study using protein-loaded liposomes.

Cameron Webb et al.

International journal of pharmaceutics, 582, 119266-119266 (2020-04-07)

Nanomedicines are well recognised for their ability to improve therapeutic outcomes. Yet, due to their complexity, nanomedicines are challenging and costly to produce using traditional manufacturing methods. For nanomedicines to be widely exploited, new manufacturing technologies must be adopted to

Modulation of Epidermal Growth Factor Release by Biopolymer-Coated Liposomes.

Gabriela Pereira Parchen et al.

Journal of pharmaceutical sciences, 109(7), 2294-2301 (2020-04-21)

This work describes the development of polysaccharide-coated liposomes to modulate the delivery of epidermal growth factor (EGF), with the aim to produce different EGF release profiles depending on the milieu of infected wounds. For this purpose, cationic liposomes were coated

Grazing-Angle Neutron Diffraction Study of the Water Distribution in Membrane Hemifusion: From the Lamellar to Rhombohedral Phase.

Shuo Qian et al.

The journal of physical chemistry letters, 9(19), 5778-5784 (2018-08-17)

The water distribution between lipid bilayers is important in understanding the role of the hydration force at different steps of the membrane fusion pathway. In this study, we used grazing-angle neutron diffraction to map out the water distribution in lipid

Autophagy impairment mediated by S-nitrosation of ATG4B leads to neurotoxicity in response to hyperglycemia

Li Y, et al.

Autophagy, 13(7), 1145-1160 (2017)

Molecular characterization of an aggregation-prone variant of alpha-synuclein used to model synucleinopathies.

Caterina Masaracchia et al.

Biochimica et biophysica acta. Proteins and proteomics, 1868(1), 140298-140298 (2019-11-05)

The misfolding and aggregation of alpha-synuclein (aSyn) are thought to be central events in synucleinopathies. The physiological function of aSyn has been related to vesicle binding and trafficking, but the precise molecular mechanisms leading to aSyn pathogenicity are still obscure.

Cationic Amphiphilic Drugs Boost the Lysosomal Escape of Small Nucleic Acid Therapeutics in a Nanocarrier-Dependent Manner.

Thijs Van de Vyver et al.

ACS nano, 14(4), 4774-4791 (2020-04-07)

Small nucleic acid (NA) therapeutics, such as small interfering RNA (siRNA), are generally formulated in nanoparticles (NPs) to overcome the multiple extra- and intracellular barriers upon in vivo administration. Interaction with target cells typically triggers endocytosis and sequesters the NPs

Preclinical Evaluation of Liposomal C8 Ceramide as a Potent anti-Hepatocellular Carcinoma Agent.

Huiqing Lv et al.

PloS one, 11(1), e0145195-e0145195 (2016-01-05)

Hepatocellular carcinoma (HCC) remains a global health threat. The search for novel anti-HCC agents is urgent. In the current study, we synthesized a liposomal C8 ceramide, and analyzed its anti-tumor activity in pre-clinical HCC models. The liposomal C8 (ceramide) potently

Metallo-Liposomes of Ruthenium Used as Promising Vectors of Genetic Material.

José Antonio Lebrón et al.

Pharmaceutics, 12(5) (2020-05-30)

Gene therapy is a therapeutic process consisting of the transport of genetic material into cells. The design and preparation of novel carriers to transport DNA is an important research line in the medical field. Hybrid compounds such as metallo-liposomes, containing

Selective organ targeting (SORT) nanoparticles for tissue-specific mRNA delivery and CRISPR-Cas gene editing.

Qiang Cheng et al.

Nature nanotechnology, 15(4), 313-320 (2020-04-07)

CRISPR-Cas gene editing and messenger RNA-based protein replacement therapy hold tremendous potential to effectively treat disease-causing mutations with diverse cellular origin. However, it is currently impossible to rationally design nanoparticles that selectively target specific tissues. Here, we report a strategy

Role of Toluidine Blue-O Binding Mechanism for Photooxidation in Bioinspired Bacterial Membranes.

Alexandre M Almeida et al.

Langmuir : the ACS journal of surfaces and colloids, 35(51), 16745-16751 (2019-11-21)

The alarming increase in bacterial resistance to antibiotics has demanded new strategies for microbial inactivation, which include photodynamic therapy whose activity relies on the photoreaction damage to the microorganism membrane. Herein, the binding mechanisms of the photosensitizer toluidine blue-O (TBO)

2/4