25-90 nm1
Chemical composition: 88-95% lipid and 5-12% protein2
References
1. Mills, G.L., et al., A Guidebook to Lipoprotein
Technique, Elsevier:Amsterdam, New York and Oxford,
1984: p. 3.
2. Olivecrona
commercial reagent contains 93-98% H2SO4, the balance being water. Its effective
concentration is 18 M (36N).
Freezing point: Anhydrous sulfuric acid freezes at 10°C; 98% sulfuric acid freezes at +3°
Applied 500 �l # �90%
Table 2. Effect of sample volume on DNA recovery.
% recovery of
1 �g DNA
% recovery of
10 �g DNA
Sample 100 �l 60 – 70% �99%
Volume 250
lysate or purified
enzyme solution in a well.
2. Add the 90 µl of Buffer to the well.
3. Start the reaction with the addition of 10 µl of the
2 mM substrate solution using
apoptosis
trifluoroacetate salt induction in intact hematopoietic cell lines.
((L-Asp)2 Rhodamine 110-TFA)
Minimum 98%
Bisbenzimide H 33258 Membrane-permeable, fluorescent DNA stain with low cytotoxicity