and Elute Results
Purity levels over 98% and an economic recovery could not be achieved using the standard bind and
elute technique (see Figure 1).
100%
90%
80%
70%
60%
50%
40%
5 mg € 90
Src1, GST-Fusion Protein, Active, Human,
Recombinant, S. frugiperda
539688 A full-length recombinant human Src1 with an N-terminal GST-fusion protein. Specific
activity: ≥98 nmol/min
acid 98-100% for analysis EMSURE® ACS, Reag. Ph Eur (1.00264)
Mobile phase: A: 0.1% formic acid in Milli-Q water
B: 0.1% formic acid in acetonitrile
Time
(min)
A
(%)
B
(%)
0 00 90 10
impurity profiling method is a gradient method starting at 98% phosphate buffer with aThe USP impurity profiling method is a gradient method starting at 98% phosphate buffer with a
65 minute cycle time.
Merck
25-90 nm1
Chemical composition: 88-95% lipid and 5-12% protein2
References
1. Mills, G.L., et al., A Guidebook to Lipoprotein
Technique, Elsevier:Amsterdam, New York and Oxford,
1984: p. 3.
2. Olivecrona