Impaired in
Mice Bearing a Targeted Mutation in the Protein
Phosphatase 1cγ. Gene. Dev. Biol., 205, 98-110
(1999).
3. Dawson, J.F., et al., Mutation of the toxin binding
site of PP-1c: comparison with
References
1. ISCO Staff, ISCO Tables, 10th edition, p. 35 (1989)
2. Dahlberg, A.E., et al., J. Mol. Biol. 41, 139 (1969)
1/98
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich
ionic strength, with pH 7-9.1
A4046 Page 2 of 3
04/07/98 - CKV
Suggested protocol for binding nucleosides:8
Equilibrate a 1.5- to 2-mL column with 50 mM
ionic strength, with pH 7-9.1
A4046 Page 2 of 3
04/07/98 - CKV
Suggested protocol for binding nucleosides:8
Equilibrate a 1.5- to 2-mL column with 50 mM
ionic strength, with pH 7-9.1
A4046 Page 2 of 3
04/07/98 - CKV
Suggested protocol for binding nucleosides:8
Equilibrate a 1.5- to 2-mL column with 50 mM
shaking speed of 90 - 100 rpm.
Cultures were passaged twice per week (5 x 106 cells/mL) and
were considered fully adapted after 6 passages, as indicated
by sustained viabilities above 90%, and at least
156-159 (1987).
2. Puissant, C. and Houdebine, L.M., BioTechniques,
8, p. 148-149 (1990).
3. Kedzierski, W. and Porter, J.C., BioTechniques, 10,
p. 210-214 (1991).
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Chem., 8,
249-252 (1997).
3. Chmura, A. J., et al., Proc. Natl. Acad. Sci. USA,
98, 8480-8484 (2001).
AGW/MAM 11/01
Sigma brand products are sold through Sigma-Aldrich, Inc.
and found negative for HBsAg
and antibody to HIV.
Refer to material safety data sheet (MSDS).
1/98
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich, Inc. warrants that its
slight turbidity occurs upon prolonged
storage clarify the solution by centrifugation before use.
6/98
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich, Inc. warrants that
Code O 9762
Storage Temperature −20 °C
CAS #: 2601−90−3
Synonym: N-(1-oxo-9-octadecenyl)-(Z)-glycine
Product Description
Purity minimum 98% (TLC)
Molecular formula C20H37NO3
Formula weight 339.5
calcium probe
with properties similar to FURA 2 (Cat. No. 344900).
Exhibits greater hydrophobicity and therefore associates
with membranes more than FURA 2. Purity: >90% by
HPLC. M.W. 946.1.
Cat. No. 341520
MONOCLONAL ANTI-Cdk6, CLONE DCS-90
Mouse Ascites Fluid
Product Number C 8343
Product Description
Monoclonal Anti-Cdk6 (mouse IgG1 isotype) is derived
from the DCS-90 hybridoma produced by the fusion
Standard Curve Preparation section.
2. Mix 10 µL of the standards from Colorimetric
Procedure with 90 µL of purified according
to Table 2.
Table 2.
Preparation of Fluorometric
to the highest dilution of
Protein Concentration: 90 mg/ml by Biuret.
Reconstitution and Storage
To one vial of lyophilized powder add 2 ml of deionized
water. Rotate vial gently until powder
to remove the bound fatty
acids. After Lipidex 1000 chromatography, at least
90% of the fatty acids are removed and 90-94%
albumin is recovered. Phospholipids, cholesterol and
steriods can also be
and Holmgren A., J. Biol. Chem.,
265, 9114-9120 (1990).
9. Makino, Y., et al., J. Clin. Invest., 98, 2469-2477
(1996).
10. Tagaya, Y., et al., EMBO J., 8, 757-764 (1989).
11. Ericson, M., et al
Chem. Rev.,
98, 2045-2076 (1998).
10. Uekama, K., et al., Cyclodextrin drug carrier
systems. Chem. Rev., 98, 2045 (1998).
11. Yaksh, T. L., et al., The utility of
2-hydroxypropyl-β-
slight turbidity occurs upon prolonged
storage clarify the solution by centrifugation before use.
7/98
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich
4.7%. The other 9 compounds
had recovery between 70% to 84%, with average RSD of 7.8%.
Together, 98 of 102 compounds in this study have the recovery
rates falling into the range of 70% to 130%, required