93615

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Functional and structural characterization of Vibrio cholerae extracellular serine protease B, VesB.

Shilpa Gadwal et al.

The Journal of biological chemistry, 289(12), 8288-8298 (2014-01-25)

The chymotrypsin subfamily A of serine proteases consists primarily of eukaryotic proteases, including only a few proteases of bacterial origin. VesB, a newly identified serine protease that is secreted by the type II secretion system in Vibrio cholerae, belongs to

[Identification of the epitope of von Willebrand factor recognized by monoclonal antibody SZ-125 with immune-affinity mass spectrometry].

Xuemei Li et al.

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology, 29(3), 284-286 (2013-05-07)

To identify the epitope of von Willebrand factor (vWF) recognized by monoclonal antibody SZ-125 (mAb SZ-125) using immune-affinity matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) assay in combination with peptide synthesis and amino acid site-mutagenesis technology. Recombinant vWF A3

Rotavirus replication in intestinal cells differentially regulates integrin expression by a phosphatidylinositol 3-kinase-dependent pathway, resulting in increased cell adhesion and virus yield.

Peter Halasz et al.

Journal of virology, 82(1), 148-160 (2007-10-19)

Changes in the interactions between intestinal cells and their surrounding environment during virus infection have not been well documented. The growth and survival of intestinal epithelial cells, the main targets of rotavirus infection, are largely dependent on the interaction of

Bacillus thuringiensis Cry1A toxins are versatile proteins with multiple modes of action: two distinct pre-pores are involved in toxicity.

Isabel Gómez et al.

The Biochemical journal, 459(2), 383-396 (2014-01-25)

Cry proteins from Bacillus thuringiensis are insecticidal PFTs (pore-forming toxins). In the present study, we show that two distinct functional pre-pores of Cry1Ab are formed after binding of the protoxin or the protease-activated toxin to the cadherin receptor, but before

Zymogen activation confers thermodynamic stability on a key peptide bond and protects human cationic trypsin from degradation.

András Szabó et al.

The Journal of biological chemistry, 289(8), 4753-4761 (2014-01-10)

Human cationic trypsinogen, precursor of the digestive enzyme trypsin, can be rapidly degraded to protect the pancreas when pathological conditions threaten, while trypsin itself is impressively resistant to degradation. For either form, degradation is controlled by two necessary initial proteolytic

Effects of secondary interactions on the kinetics of peptide and peptide ester hydrolysis by tissue kallikrein and trypsin.

F Fiedler

European journal of biochemistry, 163(2), 303-312 (1987-03-02)

Kinetic constants for the hydrolysis by porcine tissue beta-kallikrein B and by bovine trypsin of a number of peptides related to the sequence of kininogen (also one containing a P2 glycine residue instead of phenylalanine) and of a series of

The structure of human microplasmin in complex with textilinin-1, an aprotinin-like inhibitor from the Australian brown snake.

Emma-Karin I Millers et al.

PloS one, 8(1), e54104-e54104 (2013-01-22)

Textilinin-1 is a Kunitz-type serine protease inhibitor from Australian brown snake venom. Its ability to potently and specifically inhibit human plasmin (K(i) = 0.44 nM) makes it a potential therapeutic drug as a systemic anti-bleeding agent. The crystal structures of

Dulbecco's modified eagle's medium and minimum essential medium--which one is more preferred for establishment of Caco-2 cell monolayer model used in evaluation of drug absorption?

Xiu-Wen Wu et al.

Die Pharmazie, 68(10), 805-810 (2013-11-28)

The Caco-2 cell monolayer model is widely used in drug absorption studies. Dulbecco's Modified Eagle's Medium (DMEM) and Minimum Essential Medium (MEM) have been used alternatively in the development of this model, although they are different in composition which may

Cytokeratin 8-MHC class I interactions: a potential novel immune escape phenotype by a lymph node metastatic carcinoma cell line.

Ming-Syue Wu et al.

Biochemical and biophysical research communications, 441(3), 618-623 (2013-11-05)

Defective human leukocyte antigen (HLA) class I expression in malignant cells facilitates their escape from destruction by CD8(+) cytotoxic T lymphocytes. In this study, a post-translational mechanism of HLA class I abnormality that does not involve defects in the HLA

Comparative activation requirements of human peripheral blood, spleen, and lymph node B cells.

D F Jelinek et al.

Journal of immunology (Baltimore, Md. : 1950), 139(4), 1005-1013 (1987-08-15)

Human peripheral blood, spleen, and lymph node B cells were stimulated with Cowan I Staphylococcus aureus (SA) or F(ab')2 fragments of anti-mu antibody (anti-mu) and various lymphokines and were analyzed for proliferation and generation of Ig-secreting cells (ISC). SA alone

Increased proteolysis of collagen in an in vitro tensile overload tendon model.

Thomas L Willett et al.

Annals of biomedical engineering, 35(11), 1961-1972 (2007-09-04)

Presently, there is a lack of fundamental understanding regarding changes in collagen's molecular state due to mechanical damage. The bovine tail tendon (BTT; steers approximately 30 months) was characterized and used as an in vitro model for investigating the effect

Dynamic changes in histone modifications precede de novo DNA methylation in oocytes.

Kathleen R Stewart et al.

Genes & development, 29(23), 2449-2462 (2015-11-21)

Erasure and subsequent reinstatement of DNA methylation in the germline, especially at imprinted CpG islands (CGIs), is crucial to embryogenesis in mammals. The mechanisms underlying DNA methylation establishment remain poorly understood, but a number of post-translational modifications of histones are

Beneficial effect of the bioflavonoid quercetin on cholecystokinin-induced mitochondrial dysfunction in isolated rat pancreatic acinar cells.

Heike Weber et al.

Canadian journal of physiology and pharmacology, 92(3), 215-225 (2014-03-07)

The pathogenesis of acute pancreatitis (AP) is still poorly understood. Thus, a reliable pharmacological therapy is currently lacking. In recent years, an impairment of the energy metabolism of pancreatic acinar cells, caused by Ca(2+)-mediated depolarization of the inner mitochondrial membrane

Characterization of growth suppressive functions of a splice variant of cyclin D2.

Karim Wafa et al.

PloS one, 8(1), e53503-e53503 (2013-01-18)

We have recently cloned a novel splice variant of cyclin D2 termed as cycD2SV. CycD2SV overexpression in several immortalized cell lines led to formation of ubiquitinated protein aggregates accompanied by a significant decrease in cell proliferation. Based on immuno co-localization

Suppression of astrovirus replication by an ERK1/2 inhibitor.

Lindsey A Moser et al.

Journal of virology, 82(15), 7475-7482 (2008-05-30)

Human astroviruses are nonenveloped, positive-sense single-strand RNA viruses associated with self-limiting diarrhea. Although they are recognized as a leading cause of disease in young children, the cellular factors involved in astrovirus replication are not well defined. The extracellular signal-regulated kinase

Structural modeling and physicochemical characterization provide evidence that P66 forms a β-barrel in the Borrelia burgdorferi outer membrane.

Melisha R Kenedy et al.

Journal of bacteriology, 196(4), 859-872 (2013-12-10)

The Borrelia burgdorferi outer membrane (OM) contains numerous surface-exposed lipoproteins but a relatively low density of integral OM proteins (OMPs). Few membrane-spanning OMPs of B. burgdorferi have been definitively identified, and none are well characterized structurally. Here, we provide evidence

A vast amount of enzyme activity fails to be absorbed within the human pancreas: implications for cost-effective islet isolation procedures.

Andrew S Friberg et al.

Transplantation, 95(6), e36-e38 (2013-03-20)

Bridging across the ampulla with metal stents: evidences for intestinal bile reflux.

Feng Wen et al.

Hepato-gastroenterology, 60(128), 1903-1905 (2014-04-11)

To investigate the early intestinal bile reflux following the implantation of metal stent across the ampulla and the mechanism of reflux cholangitis. Twenty-three patients with implantation of metal stent across the ampulla were recruited. Prior to the implantation, the white

Matrix-assisted laser desorption/ionization imaging protocol for in situ characterization of tryptic peptide identity and distribution in formalin-fixed tissue.

Ove J R Gustafsson et al.

Rapid communications in mass spectrometry : RCM, 27(6), 655-670 (2013-02-19)

Matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry provides the means to map the in situ distribution of tryptic peptides in formalin-fixed clinical tissue samples. The ability to analyze clinical samples is of great importance to further developments in the imaging

Improvement of the plaque technique for human rotaviruses: effect of fetal bovine serum, acetyltrypsin and agar.

Y Imamura et al.

The Kurume medical journal, 38(4), 251-258 (1991-01-01)

Dilution of viruses, which were previously treated with 10 micrograms acetyltrypsin/ml, in MEM supplemented with 2.5% fetal bovine serum caused little damage of cell monolayers resulting in the formation of discrete plaques. When agar and acetyltrypsin were added to the

Direct differentiation of A-ring single attachment versus A- and D-ring double attachment of phycoerythrobilin chromophores to phycobiliproteins using MALDI mass spectrometry.

M Nazim Boutaghou et al.

Journal of mass spectrometry : JMS, 48(2), 187-192 (2013-02-05)

Bilin chromophore attachment to phycobiliproteins is an enzyme-catalyzed post-translational modification process. Bilin-lyases attach a bilin chromophore to their cognate protein through a thioether bond between the chromophore and a cysteine moiety. Bilin chromophores are attached to their phycobiliproteins through the

Stability and conformational change of methoxypolyethylene glycol modification for native and unfolded trypsin.

Wei Liu et al.

Food chemistry, 146, 278-283 (2013-11-02)

The effect of succinimidyl carbonates activated methoxypolyethylene glycol (mPEG-SC) on the catalytic properties and conformation of native trypsin and dynamic high-pressure microfluidisation (DHPM) induced unfolded trypsin was studied. The thermal stability of unfolded trypsin was enhanced more significantly than that

Improvement of the enzyme performance of trypsin via adsorption in mesoporous silica SBA-15: hydrolysis of BAPNA.

Shanshan Li et al.

Molecules (Basel, Switzerland), 18(1), 1138-1149 (2013-01-18)

The enzymatic performance of trypsin in hydrolysis of N-α-benzoyl-DL-arginine-4-nitroanilide (BAPNA) was improved by adsorption on Santa Barbara Amorphous (SBA)-15 mesoporous silica. The optimal immobilization conditions were screened and the properties of immobilized enzyme have also been studied. Under the optimal

Non-optimal codon usage affects expression, structure and function of clock protein FRQ.

Mian Zhou et al.

Nature, 495(7439), 111-115 (2013-02-19)

Codon-usage bias has been observed in almost all genomes and is thought to result from selection for efficient and accurate translation of highly expressed genes. Codon usage is also implicated in the control of transcription, splicing and RNA structure. Many

Proteomic analysis reveals that Adh1p is involved in a synergistic fluconazole and tetrandrine mechanism against Candida albicans.

Xiao-Li Zhang et al.

Die Pharmazie, 68(12), 951-954 (2014-01-10)

We previously showed that Adh1p participates in fluconazole (FLC) resistance in Candida albicans through a mechanism that may involve efflux pumps. We also found that the concomitant use of tetrandrine (TET) and FLC provided a synergistic action against C. albicans

Sarah Melissa P Jacobo et al.

Molecular and cellular biology, 33(10), 1976-1990 (2013-03-13)

Synonymous single nucleotide polymorphisms (SNPs) within a transcript's coding region produce no change in the amino acid sequence of the protein product and are therefore intuitively assumed to have a neutral effect on protein function. We report that two common

Conformational rearrangements of RIG-I receptor on formation of a multiprotein:dsRNA assembly.

Simone A Beckham et al.

Nucleic acids research, 41(5), 3436-3445 (2013-01-18)

The retinoic acid inducible gene-I (RIG-I)-like family of receptors is positioned at the front line of our innate cellular defence system. RIG-I detects and binds to foreign duplex RNA in the cytoplasm of both immune and non-immune cells, and initiates

The effect of amniotic membrane de-epithelialization method on its biological properties and ability to promote limbal epithelial cell culture.

Ting Zhang et al.

Investigative ophthalmology & visual science, 54(4), 3072-3081 (2013-04-13)

We characterized the de-epithelialized human amniotic membrane (HAM), and compared cell attachment and proliferation efficiencies. HAM was de-epithelialized by 20% ethanol (AHAM), 1.2 U/mL Dispase (DHAM), 0.02% EDTA (EHAM), 0.25% trypsin-EDTA (THAM), and 5 M urea (UHAM), respectively, followed by

Blocked transcription through KvDMR1 results in absence of methylation and gene silencing resembling Beckwith-Wiedemann syndrome.

Vir B Singh et al.

Development (Cambridge, England), 144(10), 1820-1830 (2017-04-22)

The maternally methylated KvDMR1 ICR regulates imprinted expression of a cluster of maternally expressed genes on human chromosome 11p15.5. Disruption of imprinting leads to Beckwith-Wiedemann syndrome (BWS), an overgrowth and cancer predisposition condition. In the majority of individuals with BWS

Elements of coevolution in biological sequences.

Olivier Rivoire

Physical review letters, 110(17), 178102-178102 (2013-05-18)

Studies of coevolution of amino acids within and between proteins have revealed two types of coevolving units: coevolving contacts, which are pairs of amino acids distant along the sequence but in contact in the three-dimensional structure, and sectors, which are

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