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Tomoko Yamaguchi et al.
Stem cells and development, 22(5), 726-734 (2012-10-11)
Mast cells play important roles in the pathogenesis of allergic diseases. They are generally classified into 2 phenotypically distinct populations: connective tissue-type mast cells (CTMCs) and mucosal-type mast cells (MMCs). The number of mast cells that can be obtained from
Lauren N M Hayward et al.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 31(4), 567-573 (2012-11-21)
Assessment of the early stages of fracture healing via X-rays and computed tomography is limited by the low radio-opacity of cartilage. We validated a method of contrast-enhanced computed tomography (CECT) for non-destructive identification of cartilage within a healing fracture callus.
Juryun Kim et al.
Journal of Korean medical science, 30(3), 233-239 (2015-03-03)
Eupatilin is the main active component of DA-9601, an extract from Artemisia. Recently, eupatilin was reported to have anti-inflammatory properties. We investigated the anti-arthritic effect of eupatilin in a murine arthritis model and human rheumatoid synoviocytes. DA-9601 was injected into
Alexandra Gonçalves et al.
Acta biomaterialia, 7(4), 1644-1652 (2010-12-07)
Cartilage tissue engineering (TE) typically involves the combination of a 3-D biodegradable polymeric support material, with primary chondrocytes or other cell types able to differentiate into chondrocytes. The culture environment in which cell-material constructs are created and stored is an
Quantification of biofilm biomass by staining: Non-toxic safranin can replace the popular crystal violet
Ommen, et al.
Journal of Microbiological Methods, 141, 87-89 (2017)
Anja Eichner et al.
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 14(2), 387-396 (2014-11-26)
Highly resistant endospores may cause severe problems in medicine as well as in the food and packaging industries. We found that bacterial endospores can be inactivated quickly with reactive oxygen species (ROS) that were generated by a new generation of
Jan M Suski et al.
Methods in molecular biology (Clifton, N.J.), 810, 183-205 (2011-11-08)
Mitochondria are considered as the main source of reactive oxygen species (ROS) in the cell. For this reason, they have been recognized as a source of various pathological conditions as well as aging. Chronic increase in the rate of ROS
Jan Philipp Krüger et al.
Annals of biomedical engineering, 42(12), 2562-2576 (2014-08-30)
In cartilage regeneration, bio-activated implants are used in stem and progenitor cell-based microfracture cartilage repair procedures. Our aim was to analyze the chondrogenic potential of freeze-dried resorbable polymer-based polyglycolic acid (PGA) scaffolds bio-activated with transforming growth factor-β3 (TGFB3) on human
Liliana Rodrigues et al.
Antimicrobial agents and chemotherapy, 57(2), 751-757 (2012-11-21)
Efflux pumps are membrane proteins capable of actively transporting a broad range of substrates from the cytoplasm to the exterior of the cell. Increased efflux activity in response to drug treatment may be the first step in the development of
G J Eaton et al.
Cell death & disease, 5, e1522-e1522 (2014-11-14)
Endochondral ossification is the result of chondrocyte differentiation, hypertrophy, death and replacement by bone. The careful timing and progression of this process is important for normal skeletal bone growth and development, as well as fracture repair. Apoptosis Signal-Regulating Kinase 1
Yeonsue Jang et al.
In vitro cellular & developmental biology. Animal, 51(2), 142-150 (2014-11-02)
Bone marrow concentration (BMC) is the most recognized procedure to prepare mesenchymal stem cells for cartilage regeneration. However, bone marrow aspiration is highly invasive and results in low stem cell numbers. Recently, adipose tissue-derived stromal vascular fraction (AT-SVF) was studied
Conn's Biological Stains
Kiernan & Horobin
Conn?s Biological Stains (2002)
Alexandra I Sorochkina et al.
PloS one, 7(7), e41919-e41919 (2012-08-23)
Limited uncoupling of oxidative phosphorylation could be beneficial for cells by preventing excessive generation of reactive oxygen species. Typical uncouplers are weak organic acids capable of permeating across membranes with a narrow gap between efficacy and toxicity. Aimed at designing
Heather A Cole et al.
Arthritis & rheumatology (Hoboken, N.J.), 66(8), 2222-2233 (2014-03-26)
Osteoporosis is a skeletal disorder characterized by low bone mass and increased bone fragility associated with aging, menopause, smoking, obesity, or diabetes. Persistent inflammation has been identified as an instigating factor in progressive bone loss. In addition to the role
Holly A Leddy et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 28(6), 2525-2537 (2014-03-01)
Point mutations in the calcium-permeable TRPV4 ion channel have been identified as the cause of autosomal-dominant human motor neuropathies, arthropathies, and skeletal malformations of varying severity. The objective of this study was to determine the mechanism by which TRPV4 channelopathy
Manos Stefanakis et al.
Spine, 37(22), 1883-1891 (2012-06-19)
Mechanical and biochemical analyses of cadaveric and surgically removed discs. To test the hypothesis that fissures in the annulus of degenerated human discs are mechanically and chemically conducive to the ingrowth of nerves and blood vessels. Discogenic back pain is
I L Erokhina et al.
Tsitologiia, 53(1), 39-43 (2011-04-09)
Multifunctional granular mast cells (MCs) are involved in various pathological processes. The response of MC populations of myocardium, pericardium and lung to pulmonary hypertension (PH) has been studies 8 weeks after injection of monocrotaline. Five intact and five experimental rats
Cecilia Granéli et al.
Stem cell research, 12(1), 153-165 (2013-11-19)
Today, the tool that is most commonly used to evaluate the osteogenic differentiation of bone marrow stromal cells (BMSCs) in vitro is the demonstration of the expression of multiple relevant markers, such as ALP, RUNX2 and OCN. However, as yet
Tao Qiu et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 30(2), 309-317 (2014-09-10)
Longitudinal growth of postnatal bone requires precise control of growth plate cartilage chondrocytes and subsequent osteogenesis and bone formation. Little is known about the role of angiogenesis and bone remodeling in maintenance of cartilaginous growth plate. Parathyroid hormone (PTH) stimulates
Yee Suan Poo et al.
Journal of virology, 88(12), 6862-6872 (2014-04-04)
Chikungunya virus (CHIKV) is a member of a globally distributed group of arthritogenic alphaviruses that cause weeks to months of debilitating polyarthritis/arthralgia, which is often poorly managed with current treatments. Arthritic disease is usually characterized by high levels of the
Pengfei Hu et al.
International journal of clinical and experimental pathology, 7(10), 6575-6584 (2014-11-18)
Cordycepin is widely used as for its various pharmacological activities, such as anti-inflammation, anti-angiogenesis, anti-aging, anti-tumor and anti-proliferation. However, the precise role of cordycepin on chondrocytes is not clear. In the present study, we examined the inhibitory effects of cordycepin
Xiaowei Mao et al.
Chemical communications (Cambridge, England), 48(40), 4851-4853 (2012-04-14)
p-Sulfonated calix[6]arene modified graphene has been firstly synthesized and provided as a 'turn on' probe for L-carnitine through monitoring the fluorescence signal both in vitro and in living cells.
Da-sheng Lin et al.
International journal of medical sciences, 9(2), 178-183 (2012-02-24)
The aim of this study was to observe the effects of a sliding plate on the morphology of the epiphyseal plate in goat distal femur. Eighteen premature female goats were divided randomly into sliding plate, regular plate and control groups.
Jennifer Fischer et al.
Stem cells and development, 23(20), 2513-2523 (2014-05-20)
Phenotype instability and premature hypertrophy prevent the use of human mesenchymal stromal cells (MSCs) for cartilage regeneration. Aim of this study was to investigate whether intermittent supplementation of parathyroid hormone-related protein (PTHrP), as opposed to constant treatment, can beneficially influence
Finely tuned temperature-controlled cargo release using paraffin-capped mesoporous silica nanoparticles.
Elena Aznar et al.
Angewandte Chemie (International ed. in English), 50(47), 11172-11175 (2011-09-29)
Christos Chinopoulos et al.
Methods in molecular biology (Clifton, N.J.), 793, 311-324 (2011-09-14)
Recent discoveries have brought mitochondria functions in focus of the neuroscience research community and greatly stimulated the demand for approaches to study mitochondria dysfunction in neurodegenerative diseases. Many mouse disease models have been generated, but studying mitochondria isolated from individual
Nikhil R Oak et al.
The American journal of sports medicine, 42(12), 2860-2868 (2014-09-24)
The repair and restoration of function after chronic rotator cuff tears are often complicated by muscle atrophy, fibrosis, and fatty degeneration of the diseased muscle. The inflammatory response has been implicated in the development of fatty degeneration after cuff injuries.
Improved ?-Lactam Susceptibility Against ica-Dependent Biofilm-Embedded Staphylococcus aureus by 2-Aminothiazole
Sahra Kirmusaoglu
Clinical Laboratory (2020)
Katarzyna A Wygladacz et al.
Clinical ophthalmology (Auckland, N.Z.), 10, 1423-1433 (2016-08-25)
Biotrue multipurpose solution (MPS) is a bioinspired disinfecting and conditioning solution that includes hyaluronic acid (HA) as a natural wetting agent. Previous studies demonstrated that HA sorbed from Biotrue MPS on both conventional and silicone hydrogel (SiHy) contact lens materials;
Giovanni Marfia et al.
Stem cells and development, 24(3), 320-328 (2014-09-10)
Gene expression analysis provides an effective methodology to identify clinically relevant genes implicated in intervertebral disc (IVD) pathology. The analysis of gene profile in mesenchymal stem cells (MSCs) from human herniated IVD (H-IVD) and degenerated IVD (D-IVD) has not yet
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