at �15 to �25°C.
sigma-aldrich.com
Fig. 2:Amplification of dU-containing collagen template, 1.1 kb.
A: with UNG, heat-labile treatment for 10 min at 20° C followed by 2 min denaturation
at 95°C.
with plenty of water for at least 15
minutes. Call a physician.
Cat. No. 65092B
Harleco® Safranin Stain 500 mL
Safranin O, 100% P.D.C. (6.04 g/L) SDA-3A
Alcohol 95% Ethyl, 5% Methyl Alcohol (200
Plus membrane (0.22 μm,
Polyethersulfone).
8. Measure the final pH and osmolality (pH = 7.2-7.4;
osmolality = 362 +/-15 mosmol/kg).
9. Store at 2–8 °C. Protect from light.
MilliporeSigma is the
Diethylpyrocarbonate (DEPC)-treated water
• 5 N Acetic acid
DNA Isolation:
• 8 mM NaOH
• Absolute ethanol
• 95% Ethanol
• DNAzol (available from Molecular Research
Center, Inc.)
• 1 mM EDTA
Escherichia coli BID is cleaved with caspase-8 to generate an N-terminal fragment (7 kDa) and the C-terminal
Minimum 95% (SDS-PAGE) Solution fragment (15 kDa). On size exclusion chromatography cleaved
tightly, shake
vigorously for 15 seconds, and allow to stand for
2–15 minutes at room temperature. Centrifuge the
resulting mixture at 12,000 g for 15 minutes at
2-8 C. Centrifugation separates