DESCRIPTION AND SPECIAL PHYSICA pH OF 1%
T PURIFICATION AGAROSE GEL CHARACTERISTICS L FORM (w/v)
NUMBER ELECTROPHORES SOLUTION
IS
A8551 Page 8 of 12
03/21/97 - MAC
A8412 $96% Starting material: A-4503 7.5%
precipitation
Drug Day 1 Day 2 Day 3 Day 1 Day 2 Day 3
Digoxin 94±2% 95±3% 103±16% 98±1% 96±2% 81±7%
Propranolol 96±2% 98±3% 88±11% 97±3% 96±3% 81±6%
Taxol 92±3% 92±2% 96±10% 91±2% 90±2% 99±9%
Carboxy-
peptidase A
– 97–98 µL 100 µL
Control
with
Inhibitor
2 µL
Carboxy-
peptidase A
1 µL 97 µL 100 µL
x - volume of the test sample added to the reaction
1. Prepare
has a M.W. in
the range of 97 kDa to 115 kDa.
ErbB-2 may participate in signal transduction even in
the absence of a direct ligand, because it forms
heterodimers with erbB-1/EGF-R, erbB-3, and erbB-4
should be diluted 100-fold in water.
Assay Reaction
1. Add 50 µL of water (Blank) and 50 µL of the
Calibrator into wells of a clear bottom 96 well plate.
Add 200 µL of water into the Blank
137 bp 90 82 84 97
301 bp 96 90 90 89
657 bp 97 93 91 94
1159 bp 100 93 92 94
Percent Recovery of Double-stranded DNA
0.1 ug challenge 1.0 ug challenge
Carboxypeptidase B from human pancreas (C5233) - Enzyme Assay
SPHARG01.001 Page 1 of 3
Revised: 02/20/96
SIGMA QUALITY CONTROL TEST PROCEDURE
Enzymatic Assay of CARBOXYPEPTIDASE B
(EC