red
Acidic mucosubstances light blue
Microscopy
Alcian blue solution, pH 2.5
for microscopy
1.01647.0500
For professional use only
Alcian-blue PAS staining
Staining can be carried
prepared Alcian blue solution (prepared from Cat. No. 105234).
Acetic acid 3 %
Carefully mix 485 ml of distilled water with 15 ml of acetic acid 100 %.
Alcian blue solution
Dissolve 5 g of Alcian blue
Intended purpose
Alcian blue pH 2.5 is used to stain acidic mucopolysaccharides.
This “Alcian blue staining kit, pH 2.5 - for the detection of acidic mucosub-
stances - for microscopy” is used for
Intended purpose
Alcian blue pH 1.0 is used to stain strongly sulfated mucins.
This “Alcian blue staining kit, pH 1.0 - for the detection of strongly sulfated
mucins - for microscopy” is used for
ISOSLIDE® Alcian blue, pH 2.5 Control Slides - with reference tis-
sue for the detection of acid mucosubstances in histological tissue at
+15 °C to +25 °C.
Shelf-life
The ISOSLIDE® Alcian blue, pH 2.5
red-aluminium sulfate solution 0.1% 500 ml
for microscopy
Also required:
for Alcian blue staining:
Cat. No. 101647 Alcian blue solution, pH 2.5 500 ml
for microscopy
Sample preparation
The sampling
details refer to the
package inserts for
PAS staining kit, Cat. No. 101646 or
Alcian blue solution, Cat No. 101647 or
Alcian blue 8 GX (C.I. 74240) Certistain®, Cat. No. 105234.
Technical notes
The freshly
101102 Aluminium sulfate-18-hydrate 5 kg
extra pure Ph Eur, BP
for Alcian blue staining:
Cat. No. 101647 Alcian blue solution 500 ml
for microscopy
Alternatively:
Cat. No. 100121
Result
Nuclei blue
Polysaccharides, glycogen, neutral mucopoly-
saccharides, muco- and glycoproteins, glyco-
and phospholipids, basal membrane, collagen purple
Alcian blue PAS staining
When
Result
Nuclei blue
Polysaccharides, glycogen, neutral mucopoly-
saccharides, muco- and glycoproteins, glyco-
and phospholipids, basal membrane, collagen purple
Alcian blue PAS staining
When
Result
Nuclei blue
Polysaccharides, glycogen, neutral mucopoly-
saccharides, muco- and glycoproteins, glyco-
and phospholipids, basal membrane, collagen purple
Alcian blue PAS staining
For more
Methods Mol. Biol., 31,
197-203 (1994).
7. Terry, D. E., et al., Differential use of Alcian blue
and toluidine blue dyes for the quantification and
isolation of anionic glycoconjugates from cell
cultures
standard, for
environmental analysis
1019-57-4 Analytical, Environmental, Standard
05500 Alcian Blue 8GX for microscopy (Bact., Bot., Hist.) 33864-99-2 Analytical
11640 Azophloxine for microscopy
histological tissue
Cat. No. 132647 Alcian blue staining kit, pH 1.0 1 set
for the detection of strongly sulfated
mucins
for microscopy
Cat. No. 132657 Alcian blue staining kit, pH 2.5
stain), and
many stains are sensitive to pH changes (H&E stain,
Giemsa stain from MGG stain, Alcian blue). Using pure
water with reliably low levels of ions will ensure high
quality, reproducible staining
stain), and
many stains are sensitive to pH changes (H&E stain,
Giemsa stain from MGG stain, Alcian blue). Using pure
water with reliably low levels of ions will ensure high
quality, reproducible staining
tissue for the detection
of polysaccharides in histological tissue
Cat. No. 100425 ISOSLIDE® Alcian blue, pH 2.5 25 tests
Control Slides
with reference tissue for the detection
of acid
reference tissue for the detection
of free iron in histological tissue
Cat. No. 100425 ISOSLIDE® Alcian blue, pH 2.5 25 tests
Control Slides
with reference tissue for the detection
indicada, contratinción, montaje) en material de examen histológico.
Principio
La tinción de azul alcian es una buena alternativa y puede ser combinada fácil-
mente con la reacción de ácido peryódico-Schiff
obtained. This can
be observed, for example, with stains that are highly
sensitive to pH, such as alcian blue or trichrome. Ions,
such as calcium and metals, are known to interfere
with a variety of stains
obtained. This can
be observed, for example, with stains that are highly
sensitive to pH, such as alcian blue or trichrome. Ions,
such as calcium and metals, are known to interfere
with a variety of stains
tissue for the detection
of polysaccharides in histological tissue
Cat. No. 100425 ISOSLIDE® Alcian blue, pH 2.5 25 tests
Control Slides
with reference tissue for the detection
of acid
tissue for the detection
of polysaccharides in histological tissue
Cat. No. 100425 ISOSLIDE® Alcian blue, pH 2.5 25 tests
Control Slides
with reference tissue for the detection of
acid
tissue for the detection
of polysaccharides in histological tissue
Cat. No. 100425 ISOSLIDE® Alcian blue, pH 2.5 25 tests
Control Slides
with reference tissue for the detection of
acid
tissue for the detection
of polysaccharides in histological tissue
Cat. No. 100425 ISOSLIDE® Alcian blue, pH 2.5 25 tests
Control Slides
with reference tissue for the detection
of acid
tissue for the detection
of polysaccharides in histological tissue
Cat. No. 100425 ISOSLIDE® Alcian blue, pH 2.5 25 tests
Control Slides
with reference tissue for the detection
of acid
tissue for the detection
of polysaccharides in histological tissue
Cat. No. 100425 ISOSLIDE® Alcian blue, pH 2.5 25 tests
Control Slides
with reference tissue for the detection
of acid
carbohydrate is oxidized with periodate.
The oxidized carbohydrate is either directly stained
(Alcian Blue or silver stain) or is reacted with (+)-biotin
hydrazide (Catalog Number B7639), which biotinylates