This protocol describes the detailed experimental procedure for the synthesis of an azide-modified uridine triphosphate analog and its effective incorporation into an oligoribonucleotide by in vitro transcription reactions. Furthermore, procedures for labeling azide-modified oligoribonucleotides post-transcriptionally with biophysical probes by copper(I)-catalyzed
Detection of erythrocyte membrane proteins, sialoglycoproteins, and lipids in the same polyacrylamide gel using a double-staining technique.
Dzandu JK, et al.
Proceedings of the National Academy of Sciences of the USA, 81, 1733-1737 (1984)
Synthesis of oligo-RNAs with photocaged adenosine 2'-hydroxyls.
Monitoring drug-target interactions with methods such as the cellular thermal-shift assay (CETSA) is well established for simple cell systems but remains challenging in vivo. Here we introduce tissue thermal proteome profiling (tissue-TPP), which measures binding of small-molecule drugs to proteins
A dielectrophoretic continuous flow sorter using integrated microelectrodes coupled to a channel constriction
Salomon S, et al.
Electrophoresis, 32(12), 1508-1514 (2011)
Loss of osmoregulation of OmpC porin occurs if Escherichia coli are deficient in cAMP receptor protein
Joo SH, et al.
Journal of Experimental Microbiology and Immunology, 17, 78-82 (2013)
Rapid transfer of DNA from agarose gels to nylon membranes.
Reed KC and Mann DA
Nucleic Acids Research, 13.20, 7207-7221 (1985)
The expression of ERa, OTR, cPLA(2), COX-2, and PPAR? in the cervix of the ewe during the estrous cycle.
Falchi L and Scaramuzzi RJ
Theriogenology, 79, 40-47 (2013)
The use of vital dyes during vitreoretinal surgery-chromovitrectomy
In Nature, mammalian seasonal breeders undergo spermatogenetic arrest during the non-breeding season. In the large hairy armadillo Chaetophractus villosus, testis regression initiates with immature post-meiotic germ cells sloughing into the tubule lumen and continues with the death of the remaining
Purification of human plasma fibronectin using immobilized gelatin and Arg affinity chromatography