chicken., Tissue Cell, 25, 817-824 (1993).
5. Toth, T.E., et al., Simultaneous separation and
purificiation of mononuclear and
polymorphonuclear cells from the peripheral blood
of cats., J. Virol
Procedure for the Isolation of Mononuclear Cells
(modification of method described by Fauser and
Messner 2)
1. Collect fresh bone marrow, cord blood or peripheral
blood into a tube containing an anticoagulant
tissue
sections.
IMMUNOGEN:
Human peripheral blood mononuclear cells.
APPLICATIONS:
Flow Cytometry: 1:25, use 50 µl per million cells in 100 µl of PBS buffer.
Immunohistochemistry
Whole human blood collected by standard
clinical blood evacuation tubes with EDTA,
ACD-A or heparin anticoagulant or
b. Human cell suspension (e.g., peripheral
blood mononuclear cells isolated on
Procedure for the Isolation of Mononuclear Cells (modification
of method described by Fauser and Messner 2)
1. Collect fresh bone marrow, cord blood or peripheral
blood into a tube containing an anticoagulant
Procedure for the Isolation of Mononuclear Cells
(modification of method described by Fauser and
Messner 2)
1. Collect fresh bone marrow, cord blood or peripheral
blood into a tube containing an anticoagulant
Procedure for the Isolation of Mononuclear Cells (modification
of method described by Fauser and Messner 2)
1. Collect fresh bone marrow, cord blood or peripheral
blood into a tube containing an anticoagulant
Procedure for the Isolation of Mononuclear Cells
(modification of method described by Fauser and
Messner 2)
1. Collect fresh bone marrow, cord blood or
peripheral blood into a tube containing an
Procedure for the Isolation of Mononuclear Cells (modification
of method described by Fauser and Messner 2)
1. Collect fresh bone marrow, cord blood or peripheral
blood into a tube containing an anticoagulant
. Whole human blood collected by standard clinical
blood evacuation tubes with EDTA, ACD-A or
heparin anticoagulant or
b. Human cell suspension (e.g., peripheral blood
mononuclear cells isolated on HISTOPAQUE
Whole human blood collected by standard
clinical blood evacuation tubes with EDTA,
ACD-A or heparin anticoagulant or
b. Human cell suspension (e.g., peripheral blood
mononuclear cells isolated on
. Whole human blood collected by standard
clinical blood evacuation tubes with EDTA,
ACD-A or heparin anticoagulant or
b. Human cell suspension (e.g., peripheral blood
mononuclear cells isolated on HISTOPAQUE
Whole human blood collected by standard
clinical blood evacuation tubes with EDTA,
ACD-A or heparin anticoagulant or
b. Human cell suspension (e.g. peripheral blood
mononuclear cells isolated on
Whole human blood collected by standard
clinical blood evacuation tubes with EDTA, ACD-
A or heparin anticoagulant or
b. Human cell suspension (e.g. peripheral blood
mononuclear cells isolated on
Whole human blood collected by standard
clinical blood evacuation tubes with EDTA, ACD-
A or heparin anticoagulant or
b. Human cell suspension (e.g., peripheral blood
mononuclear cells isolated on
Whole human blood collected by standard
clinical blood evacuation tubes with EDTA, ACD-
A or heparin anticoagulant or
b. Human cell suspension (e.g., peripheral blood
mononuclear cells isolated on
Whole human blood collected by standard clini-
cal blood evacuation tubes with EDTA, ACD-A
or heparin anticoagulant or
b. Human cell suspension (e.g. peripheral blood
mononuclear cells isolated on
collected by standard
clinical blood evacuation tubes with EDTA,
ACD-A or heparin anticoagulant or
b. Human cell suspension (e.g., peripheral blood
mononuclear cells isolated on HISTOPAQUE
(Product
Whole human blood collected by standard
clinical blood evacuation tubes with EDTA,
ACD-A or heparin anticoagulant or
b. Human cell suspension, e.g., peripheral blood
mononuclear cells isolated on
Whole human blood collected by standard
clinical blood evacuation tubes with EDTA,
ACD-A, or heparin anticoagulant OR
b. Human cell suspension (e.g., peripheral blood
mononuclear cells isolated on
. Whole human blood collected by standard
clinical blood evacuation tubes with EDTA,
ACD-A or heparin anticoagulant or
b. Human cell suspension (e.g., peripheral blood
mononuclear cells isolated on
HISTOPAQUE
Whole human blood collected by standard
clinical blood evacuation tubes with EDTA,
ACD-A or heparin anticoagulant or
b. Human cell suspension (e.g., peripheral blood
mononuclear cells isolated on
Whole human blood collected by standard
clinical blood evacuation tubes with EDTA, ACD-
A or heparin anticoagulant or
b. Human cell suspension (e.g. peripheral blood
mononuclear cells isolated on
Whole human blood collected by standard
clinical blood evacuation tubes with EDTA,
ACD-A or heparin anticoagulant or
b. Human cell suspension (e.g., peripheral blood
mononuclear cells isolated on
Whole human blood collected by standard
clinical blood evacuation tubes with EDTA,
ACD-A or heparin anticoagulant or
b. Human cell suspension, e.g., peripheral blood
mononuclear cells isolated on
. Whole human blood collected by standard
clinical blood evacuation tubes with EDTA,
ACD-A, or heparin anticoagulant OR
b. Human cell suspension (peripheral blood
mononuclear cells isolated on HISTOPAQUE
Whole human blood collected by standard
clinical blood evacuation tubes with EDTA,
ACD-A,or heparin anticoagulant OR
b. Human cell suspension (e.g., peripheral blood
mononuclear cells isolated on
Whole human blood collected by standard clinical
blood evacuation tubes with EDTA, ACD-A or
heparin anticoagulant or
b. Human cell suspension (e.g., peripheral blood
mononuclear cells isolated on
Whole human blood collected by standard
clinical blood evacuation tubes with EDTA,
ACD-A, or heparin anticoagulant OR
b. Human cell suspension (e.g. peripheral blood
mononuclear cells isolated
Whole human blood collected by standard
clinical blood evacuation tubes with EDTA,
ACD-A or heparin anticoagulant or
b. Human cell suspension (e.g. peripheral blood
mononuclear cells isolated