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Luis Felipe Vallejo et al.
Biotechnology and bioengineering, 85(6), 601-609 (2004-02-18)
The human gene encoding the mature form of bone morphogenetic protein-2 (hBMP-2), a dimeric disulfide-bonded protein of the cystine knot growth factor family, was expressed in recombinant Escherichia coli using a temperature-inducible expression system. The recombinant protein was produced in
Josef Ehgartner et al.
The Analyst, 139(19), 4924-4933 (2014-08-07)
A low cost imaging set-up for optical chemical sensors based on NIR-emitting dyes is presented. It is based on a commercially available 2-CCD colour near infrared camera, LEDs and tailor-made optical sensing materials for oxygen and pH. The set-up extends
Takashi Miyado et al.
Journal of chromatography. A, 1206(1), 41-44 (2008-08-12)
In order to develop a high-throughput assay for nitric oxide metabolites, nitrite (NO2-) and nitrate (NO3-), in biological fluids, we have investigated the simultaneous determination of them using an electrophoretic lab-on-a-chip (microchip capillary electrophoresis, MCE) technique. In this study, in
Determination of folate in some cereals and commercial cereal-grain products consumed in Poland using trienzyme extraction and high-performance liquid chromatography methods
Gujska E and Kuncewicz A
European Food Research and Technology, 221(1-2), 208-213 (2005)
Jun Yang et al.
Langmuir : the ACS journal of surfaces and colloids, 24(18), 10265-10272 (2008-08-20)
A universal nitric oxide (NO) generating surface is assembled via Layer-by-Layer (LbL) deposition of sodium alginate (Alg) and organoselenium modified polyethyleneimine (SePEI) on quartz and polymeric substrates. The immobilized SePEI species is capable of catalytically decomposing S-nitrosothiol species (RSNO) to
N Frančič et al.
The Analyst, 139(12), 3127-3136 (2014-05-03)
In this work we demonstrate the efficient immobilization of histidine 6-tagged organophosphate hydrolase (His6-OPH), an organophosphate-degrading enzyme, on mesoporous titania thin films. This permits the use of the biocatalyst films as efficient tools in the detection/detoxification of paraoxon. His6-OPH was
Garry Jason Forse et al.
PloS one, 10(5), e0127081-e0127081 (2015-05-21)
The Eph receptor tyrosine kinase/ephrin ligand system regulates a wide spectrum of physiological processes, while its dysregulation has been implicated in cancer progression. The human EphA3 receptor is widely upregulated in the tumor microenvironment and is highly expressed in some
Scott A Sarver et al.
Journal of chromatography. A, 1229, 268-273 (2012-02-11)
Several glycosphingolipids were labeled with the fluorphore Bodipy-Fl and analyzed using capillary electrophoresis with laser-induced fluorescence detection. GM1-, LacCer-, and Cer-Bodipy-Fl were prepared through acylation using the N-hydroxysuccinimide ester of Bodipy-Fl. Several other glycosphingolipids including GT1a-, GD1a-, GM2-, GM3-, GD3-
Michael C Breadmore et al.
Analytical chemistry, 80(16), 6373-6381 (2008-07-17)
An electroosmotic flow (EOF) controlled counterflow isotachophoretic stacking boundary (cf-ITPSB) system under field amplified conditions has been examined as a way to improve the sensitivity of anions separated by capillary zone electrophoresis. The system comprised a high concentration of a
P Doble et al.
Electrophoresis, 19(12), 2257-2261 (1998-10-07)
The suitability of relatively slow (low absolute value of mobility) coanionic buffers in background electrolytes (BGEs) for indirect photometric detection of anions by capillary electrophoresis was investigated. As a model system, 2-(cyclohexylamino)ethanesulfonic acid (CHES) was used to buffer the indirect
David G McLaren et al.
Electrophoresis, 24(17), 2887-2895 (2003-09-16)
A systematic evaluation of continuous flow-counterbalanced capillary electrophoresis for microscale preparative applications is presented. The success of the technique was found to depend on the specific nature of the analyte and background electrolyte and was most heavily influenced by factors
Use of a reversible polyacrylamide gel cross-linker in western blotting for rapid transfer of a wide size range of polypeptides.
J A Airey et al.
BioTechniques, 10(5), 605-608 (1991-05-01)
M G Hartmanis et al.
Proceedings of the National Academy of Sciences of the United States of America, 84(1), 76-79 (1987-01-01)
A procedure for solubilization of the oxygen-labile, membrane-bound diol dehydratase from Clostridium glycolicum with retention of enzymatic activity is described. The procedure involves sonication of crude membrane preparations anaerobically in 0.1 M 2-(N-cyclohexylamino)ethane-sulfonic acid (CHES) buffer (pH 8.6-9.0) containing 2
A D Carraway et al.
Journal of inorganic biochemistry, 60(4), 267-276 (1995-12-01)
Electron paramagnetic resonance spectra of a number of ferric heme peptide derivatives, in aqueous-detergent and various aqueous-alcohol solvent mixtures, have been obtained using samples in the concentration range 0.1-1.0 mM. Some of these were clearly monomeric, homogeneous, mixed-ligand adducts, entirely
Jun An et al.
Science China. Life sciences, 58(8), 765-772 (2015-05-28)
A new biomimetic material for artificial blood vessel with in situ catalytic generation of nitric oxide (NO) was prepared in this study. Organoselenium immobilized polyethyleneimine as NO donor catalyst and sodium alginate were alternately loaded onto the surface of electrospun
Selvin H Edwards et al.
Electrophoresis, 23(9), 1320-1327 (2002-05-15)
Chiral separation of moderately to highly hydrophobic polychlorinated biphenyls (PCBs) using a conventional chiral micelle or a polymeric chiral surfactant, as the single chiral selector is very difficult since the hydrophobic interactions between the chiral PCB and the monomeric or
Stéphane Priet et al.
Nucleic acids research, 43(7), 3776-3788 (2015-03-18)
Giant viruses from the Mimiviridae family replicate entirely in their host cytoplasm where their genes are transcribed by a viral transcription apparatus. mRNA polyadenylation uniquely occurs at hairpin-forming palindromic sequences terminating viral transcripts. Here we show that a conserved gene
Václav Matěj Bierhanzl et al.
Analytical and bioanalytical chemistry, 407(23), 7215-7220 (2015-07-15)
A new, fast, selective, and reliable capillary electrophoresis method has been developed for analysis of selected phosphoesters (phosphoserine, phosphoethanolamine, phosphoglycerol) and phosphate. The method is based on separation of specific phosphate containing headgroups (phosphoesters) which are cleaved from the glycerol
Good's buffers as a basis for developing self-buffering and biocompatible ionic liquids for biological research
Taha M, et al.
Green Chemistry, 16(6), 3149-3159 (2014)
Hisayoshi Fukumori et al.
Bioscience, biotechnology, and biochemistry, 78(2), 202-211 (2014-07-19)
Cocoonase (CCN) which facilitates the degradation of a cocoon is recognized as a trypsin-like serine protease. In this study, CCN from the silkworm Bombyx mori was purified and comprehensively characterized. Its activity was maximal at about pH 9.8. It was
Detection of Bulky Endogenous Oxidative DNA Lesions Derived from 8, 5?-Cyclo-2?-deoxyadenosine by 32P-Postlabeling Assay
Zhou GD and Moorthy B
Current Protocols in Toxicology / Editorial Board, Mahin D. Maines (editor-in-chief) ... [Et al.], 64(1), 17-17 (2015)
Yazheng Liu et al.
Food chemistry, 194, 26-31 (2015-10-17)
Folate deficiency is linked to many diseases, some of which may have higher probability in individuals with alcohol-induced alterations in one-carbon metabolism. Our study shows that folate content in commercial wine is not related to white or red varieties, but
L M Gloss et al.
Biochemistry, 34(12), 3999-4007 (1995-03-28)
The pH dependence of Escherichia coli aspartate aminotransferase (AATase) has been investigated by the use of site-directed mutants and alternative substrates. Inhibition of the enzyme by CHES and variations in ionic strength are proposed to explain some of the qualitative
Andrew Harris et al.
Biochimica et biophysica acta, 1847(12), 1518-1529 (2015-08-12)
One of the main functions of microbial rhodopsins is outward-directed light-driven proton transport across the plasma membrane, which can provide sources of energy alternative to respiration and chlorophyll photosynthesis. Proton-pumping rhodopsins are found in Archaea (Halobacteria), multiple groups of Bacteria
R C Bray et al.
Biochemistry, 39(37), 11258-11269 (2000-09-14)
Much is unknown concerning the role of thiolate ligands of molybdenum in molybdopterin enzymes. It has been suggested that thiolate dissociation from molybdenum is part of the catalytic mechanism of bis-molybdopterin enzymes of the dimethyl sulfoxide reductase (DMSOR) family. For
P Salgado et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 992, 103-108 (2015-05-15)
The organic sulfur compound 3-mercaptopropionic acid (3-MPA) is an important thiol intermediate in organic sulfur metabolism in natural environments. It is generated during degradation of sulfur-containing amino acids (e.g. methionine) and from demethylation of dimethylsulfoniopropionate (DMSP). This pathway is an
Hemicellulase activity of antarctic microfungi
Bradner JR, et al.
Journal of Applied Microbiology, 87(3), 366-370 (1999)
Intae Kim et al.
Sensors (Basel, Switzerland), 15(6), 12513-12525 (2015-05-30)
In whole-cell based biosensors, spectrophotometry is one of the most commonly used methods for detecting organophosphates due to its simplicity and reliability. The sensor performance is directly affected by the cell immobilization method because it determines the amount of cells
Kim M Hanson et al.
Electrophoresis, 26(4-5), 943-946 (2005-02-17)
This report outlines a rapid, reproducible method for the determination of beta-asarone, a known carcinogen, using micellar electrokinetic capillary chromatography (MEKC)-UV-vis absorbance and a simple alcohol extraction. The MEKC method is based on a running buffer comprised of 100 mM
Hao Zhong et al.
The Analyst, 136(21), 4486-4491 (2011-09-29)
On-line concentration via Electrokinetic Supercharging (EKS) was used to enhance the sensitivity of the capillary electrophoretic separation of the four flavonoids naringenin, hesperetin, naringin and hesperidin. Separation conditions, including the background electrolyte pH and concentration, the length and choice of
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