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Michael L Dustin et al.
Current protocols in immunology, Chapter 18, 18-18 (2008-04-25)
Supported planar bilayers have been used in immunology research for over 25 years, including in the initial demonstrations of MHC-peptide complex functional activity and adhesion molecule activity. More recent modifications of the method have been used to measure two-dimensional affinities
K Benschop et al.
Journal of clinical microbiology, 46(12), 3965-3970 (2008-10-24)
Human parechoviruses (HPeV) are widespread pathogens belonging to the Picornavirus family. Six genotypes, which have predominantly been isolated from children, are known. Data on prevalence of HPeV genotypes are generally based on cell culture, which may underestimate the prevalence of
Christopher John Harding et al.
Nature chemical biology, 19(9), 1158-1166 (2023-06-30)
Pseudomonas aeruginosa is an opportunistic pathogen that causes serious illness, especially in immunocompromised individuals. P. aeruginosa forms biofilms that contribute to growth and persistence in a wide range of environments. Here we investigated the aminopeptidase, P. aeruginosa aminopeptidase (PaAP) from
David Arranz-Solís et al.
Frontiers in cellular and infection microbiology, 11, 621738-621738 (2021-03-09)
The severity of toxoplasmosis depends on a combination of host and parasite factors. Among them, the Toxoplasma strain causing the infection is an important determinant of the disease outcome. Type 2 strains dominate in Europe, whereas in North America type
Ferda Ari et al.
Cell biochemistry and function, 39(4), 571-583 (2021-02-21)
Despite the concerning adverse effects on tumour development, epigenetic drugs are very promising in cancer treatment. The aim of this study was to compare the differential effects of standard chemotherapy regimens (FEC: 5-fluorouracil plus epirubicine plus cyclophosphamide) in combination with
Rafael Zapata et al.
Archives of insect biochemistry and physiology, 60(2), 84-92 (2005-09-22)
Improvement of an existing meat-based diet has been obtained for rearing the generalist predator Dicyphus tamaninii (Heteroptera: Miridae). The approach followed, different from the classical addition/deletion method, was performing biochemical analysis of adult carcasses in order to have information about
Kimi Drobin et al.
Cancers, 12(3) (2020-04-03)
Nearly half of all cancers are treated with radiotherapy alone or in combination with other treatments, where damage to normal tissues is a limiting factor for the treatment. Radiotherapy-induced adverse health effects, mostly of importance for cancer patients with long-term
Determination of the protein content in rice by the digital chromatic method
SUN JP, et al.
Journal of Food Quality, 31(2), 250-263 (2008)
Effect of milk hydrolysates on inflammation markers and drug-induced transcriptional alterations in cell-based models
Nielsen D, et al.
Journal of Animal Science, 90(suppl\_4), 403-405 (2012)
Meghan A Morrissey et al.
Immunity, 53(2), 290-302 (2020-08-10)
CD47 acts as a "don't eat me" signal that protects cells from phagocytosis by binding and activating its receptor SIPRA on macrophages. CD47 suppresses multiple different pro-engulfment "eat me" signals, including immunoglobulin G (IgG), complement, and calreticulin, on distinct target
Esterase and protease activities of Bacillus spp. from afitin, iru and sonru; three African locust bean (Parkia biglobosa) condiments from Benin
Azokpota P, et al.
African Journal of Biotechnology, 5(3), 265-272 (2006)
Yi-Jin Lu et al.
PLoS genetics, 12(11), e1006409-e1006409 (2016-11-05)
Polyploidization has crucial impacts on the evolution of different eukaryotic lineages including fungi, plants and animals. Recent genome data suggest that, for many polyploidization events, all duplicated chromosomes are maintained and genome reorganizations occur much later during evolution. However, newly-formed
Rapid evolutionary repair by secondary perturbation of a primary disrupted transcriptional network.
Hsu, et al.
EMBO Reports, 24, e56019-e56019 (2023)
Mohamed Azarkan et al.
Protein and peptide letters, 27(11), 1159-1170 (2020-06-03)
Antiplatelet, anticoagulant and fibrinolytic activities of stem bromelain (EC 3.4.22.4) are well described, but more studies are still required to clearly define its usefulness as an antithrombotic agent. Besides, although some effects of bromelain are linked to its proteolytic activity
N D Richert et al.
The Journal of biological chemistry, 257(12), 7143-7150 (1982-06-25)
We recently described a method for the purification of a protein kinase related to pp60src from Rous sarcoma virus-induced rat tumors (Blithe, D. L., Richert, N. D., and Pastan, I. H. (1982) J. Biol. Chem. 257, 7135-7142). In this report
B T Kile et al.
Molecular and cellular biology, 21(18), 6189-6197 (2001-08-18)
The Asbs are a family of ankyrin repeat proteins that, along with four other protein families, contain a C-terminal SOCS box motif, which was first identified in the suppressor of cytokine signaling (SOCS) proteins. While it is clear that the
Marie-France Lusignan et al.
Biology of reproduction, 85(3), 457-464 (2011-05-20)
Milk has been used routinely as an extender for sperm preservation. Caseins, the major proteins in milk, are proposed to be the protective constituents of milk during sperm preservation. It is unclear whether the whey proteins in milk are also
Tetsuya Hori et al.
Cell reports, 33(7), 108388-108388 (2020-11-19)
CENP-A incorporation is critical for centromere specification and is mediated by the chaperone HJURP. The CENP-A-targeting domain (CATD) of CENP-A specifically binds to HJURP, and this binding is conserved. However, the binding interface of CENP-A-HJURP is yet to be understood.
Maayan Avneon et al.
Leukemia research, 33(10), 1430-1432 (2009-04-21)
We have previously shown that erythropoietin (EPO) potentiates the immune response. Analysis of various possible cellular mediators was performed on EPO-injected mice and transgenic mice overexpressing human EPO (tg6). Here we present our studies on neutrophils, peritoneal (casein induced), and
Shih-Wei Hsiang et al.
Journal of the Royal Society, Interface, 8(64), 1622-1634 (2011-04-29)
Recent advances in nerve repair technology have focused on finding more biocompatible, non-toxic materials to imitate natural peripheral nerve components. In this study, casein protein cross-linked with naturally occurring genipin (genipin-cross-linked casein (GCC)) was used for the first time to
Vera J E van Vliet et al.
PLoS pathogens, 18(12), e1011065-e1011065 (2022-12-23)
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has made it clear that combating coronavirus outbreaks benefits from a combination of vaccines and therapeutics. A promising drug target common to all coronaviruses-including SARS-CoV, MERS-CoV, and SARS-CoV-2-is the papain-like protease
Marta Montoro et al.
Insects, 11(4) (2020-04-25)
Mass rearing of insects, used both as biological control agents and for food and feed, is receiving increasing attention. Efforts are being made to improve diets that are currently in use, and to identify alternative diets, as is the case
Assay of cyclic AMP by protein kinase activation.
S E Mayer et al.
Methods in enzymology, 38, 66-73 (1974-01-01)
Marta Montoro et al.
Insect science, 28(1), 191-202 (2020-01-29)
The predatory bug, Orius majusculus (Reuter), is an important predator of thrips commercially produced for augmentative releases using the eggs of the Mediterranean flour moth Ephestia kuehniella (Zeller). In this study, we assessed the potential for using frozen adults of
Jason Yi et al.
Molecular biology of the cell, 23(5), 834-852 (2012-01-06)
Actin retrograde flow and actomyosin II contraction have both been implicated in the inward movement of T cell receptor (TCR) microclusters and immunological synapse formation, but no study has integrated and quantified their relative contributions. Using Jurkat T cells expressing
Cheryl M Armstrong et al.
Toxins, 10(11) (2018-10-27)
Amplified luminescent proximity homogenous assay-linked immunosorbent assay (AlphaLISA) is comprised of a bead-based immunoassay that is used for small molecule detection. In this study, a novel AlphaLISA was developed and optimized for the detection of Shiga-toxin 2 (Stx2). Efficacy and
Po-Hsiang Hung et al.
iScience, 26(5), 106635-106635 (2023-05-04)
Enhanced phenotypic diversity increases a population's likelihood of surviving catastrophic conditions. Hsp90, an essential molecular chaperone and a central network hub in eukaryotes, has been observed to suppress or enhance the effects of genetic variation on phenotypic diversity in response
D Metcalf et al.
Blood, 88(10), 3755-3764 (1996-11-15)
The intraperitoneal injection into mice of casein preparations containing bacteria induced a rapid accumulation of neutrophils within 3 hours due to selective release of mature cells from the bone marrow. Significant increases in the concentrations of granulocyte-macrophage colony-stimulating factor (GM-CSF)
François Chevalier et al.
Journal of agricultural and food chemistry, 58(12), 7437-7444 (2010-05-28)
Disulfide bond formation between milk protein molecules was quantified in raw and heated bovine milk using reducing and nonreducing two-dimensional electrophoresis. Analysis of protein profiles in raw milk indicated that 18% of alpha(S2)-casein, 25% of beta-lactoglobulin, and 46% of kappa-casein
Guo-Yue Wan et al.
Archives of medical science : AMS, 18(2), 376-381 (2022-03-24)
More than half of the world's population is infected with Helicobacter pylori, which may cause gastritis, peptic ulcer and even gastric cancer. The World Health Organization (WHO) has announced that H. pylori infection is a class I carcinogen and hence
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