microscopy using immersion oil, i. e. allow specimen slides to dry thoroughly
or, if necessary, cover with a cover glass, otherwise the microscopic image will
become turbid.
Reagent preparation
All
15 min
allow to run off thoroughly, dabbing the slide dry if necessary
mount the slides with e.g. Aquatex® and cover glass
microscope under UV excitation
Microscopy
4’,6-Diamidino-2-phenylindole
to clean, glass microscope slides and allow
to dry at room temperature (25 °C) for 5-16 hours.
6. Immerse the slides in the pre-cooled acetone
(−20 °C) for 20 minutes.
7. Allow slides to dry
with a cover
glass, and microscope immediately.
For storage, it is advisable to mount the specimen with a mounting medium and a
cover glass. To do this remove the cover glass after microscoping, rinse
to clean, glass microscope slides and allow
to dry at room temperature (25 °C) for 5-16 hours.
6. Immerse the slides in the pre-cooled acetone
(−20 °C) for 20 minutes.
7. Allow slides to dry
human origin, and the slide is
covered air-tight with a cover glass. The evaporation of the water causes
the mounting medium to harden, forming a solid, clear film under the cover
glass, preserving the stained
cloth.
Cover the cells with a sufficient amount of Anti-mouse-Ig-AP working solution.
– Incubate the glass slide for 30 minutes at +37°C in a humidified atmosphere.
Wash glass slides with cells
DPX
new or Entellan® new) and a cover glass and and can then be stored.
The use of immersion oil is recommended for the analysis of stained slides
with a microscopic magnification >40x.
Result
Nuclei
human origin, and the slide is
covered air-tight with a cover glass. The evaporation of the water causes
the mounting medium to harden, forming a solid, clear film under the cover
glass, preserving the stained
human origin, and the slide is
covered air-tight with a cover glass. The evaporation of the water causes
the mounting medium to harden, forming a solid, clear film under the cover
glass, preserving the stained
Cover the cells with a sufficient amount of Anti-mouse-Ig-fluorescein working solution.
– Incubate the glass slide for 30 minutes at +37°C in a humidified atmosphere.
Wash glass slides with
onto a
glass slide and cover the cells with a glass cover
slip.
7. Observe the cells under a fluorescent microscope
equipped with the appropriate filters.
Note: If using an inverted microscope, 100
Xylene or Neo-Clear® 5 min
Mount the Neo-Clear®-wet slides with Neo-Mount® or the xylene-wet
slides with e. g. Entellan® new and cover glass.
After dehydration (ascending alcohol series) and clarification
blocking serum/0.25% Triton X-100 in
DPBS)
16. Slide mounting media (with anti-fade
reagent) and cover glasses, if
appropriate
17. Microscope/image acquisition system
(for phase contrast and
blocking serum/0.25% Triton X-100 in
DPBS)
16. Slide mounting media (with anti-fade
reagent) and cover glasses, if
appropriate
17. Microscope/image acquisition system
(for phase contrast and
hydrophobic cover slips for hybridization, in situ, PCR,
and hybridization to genomic arrays on glass slides.
Features and Benefits
• Constant probe concentration — Unlike glass cover slips, Hybri-
® 5 min
Mount the Neo-Clear®-wet slides with Neo-Mount® or the xylene-wet
slides with DPX new and cover glass.
with sodium thiosulfate solution
Slide with paraffin section
1 Distilled
Xylene or Neo-Clear® 5 min
Mount the Neo-Clear®-wet slides with Neo-Mount® or the xylene-wet
slides with e. g. Entellan® new and cover glass.
After dehydration (ascending alcohol series) and clarification
for the analysis of stained slides with a
microscopic magnification >40x.
Result
Glycogen red
Mucous, fibrin, amyloid pink to red
Technical notes
The microscope used should meet the requirements
Xylene or Neo-Clear® 5 min
Mount the Neo-Clear®-wet slides with Neo-Mount® or the xylene-wet
slides with Entellan® new and cover glass.
After dehydration (ascending alcohol series) and clarification
, DPX new or Entellan® new) and a
cover glass and can then be stored.
The use of immersion oil is recommended for the analysis of stained slides
with a microscopic magnification >40x.
Result
Nuclei
blocking serum/0.25% Triton X-100 in
DPBS)
18. Slide mounting media (with anti-fade
reagent) and cover glasses, if
appropriate
19. Microscope/image acquisition system
(for phase contrast and
agents (e. g.
Entellan® new) and a cover glass and can then be stored.
The use of immersion oil is recommended for the analysis of stained slides
with a microscopic magnification >40x.
Result
Cyanophilic
burner
Glass slides
Cover slips
Timer accurate to 15 seconds per minute
Absorbent paper
Immersion oil
Mounting media
Microscope, with oil immersion lens
Procedure: Flood Slide Method
clarification with xylene or
Neo-Clear®, histological slides can be covered with non-aqueous mounting
agents (e. g. Entellan® new, Neo-Mount®) and a cover glass and can then be
stored.
Result
Reticular
analysis of stained slides
with a microscopic magnification >40x.
Result
Free iron (Fe3+) intensive blue granules
Nuclei pale red
Cytoplasm tender pink
Technical notes
The microscope used should meet
freezing
• Glass slides
• Cover glasses (Catalog Number C9802)
• Fluorescent microscope equipped with appropriate
filters. The kit was developed using Olympus IX71
inverted microscope equipped with
remains stable for about 3
days, covered with immersion oil only for a few hours.
The use of immersion oil is recommended for the analysis of stained slides with a
microscopic magnification >40x.
Technical
DPX new or Entellan® new) and a cover
glass and and can then be stored.
The use of immersion oil is recommended for the analysis of stained slides with a
microscopic magnification >40x.
Result
Cell
with xylene or
Neo-Clear®, histological slides can be covered with non-aqueous mounting
agents (e. g. DPX new, Entellan® new, Neo-Mount®) and a cover glass and
can then be stored.
Result
Staining