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cover glass for microscope slide
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关键词:'cover glass for microscope slide'
显示 1-30 共 87 条结果 关于 "cover glass for microscope slide" 范围 论文
Jie Jiang et al.
Analytical chemistry, 87(16), 8057-8062 (2015-07-24)
Ambient ionization mass spectrometry is achieved in a simple manner by loading a sample solution onto a corner of a microscope cover glass positioned in front of the inlet to a mass spectrometer and applying a high voltage to the
I D da Silva-Neto
Revista brasileira de biologia, 60(3), 451-459 (2001-02-24)
The research on ciliates, flagelates and opalinates have been widespread by the utilization of techniques employing silver impregnation (protargol), modified by several authors. However, these are time consuming and its results are variable. The present work is a variant of
A Sawaguchi et al.
Journal of microscopy, 234(2), 113-117 (2009-04-29)
The goal of specimen preparation for transmission electron microscopy is to obtain high-quality ultra-thin sections with which we can correlate cellular structure to physiological function. In this study, we newly developed a capsule-supporting ring that can be useful for resin
Stella H North et al.
Analytical chemistry, 82(1), 406-412 (2009-12-17)
Glass microscope slides are considered by many as the substrate of choice for microarray manufacturing due to their amenability to various surface chemistry modifications. The use of silanes to attach various functional groups onto glass slides has provided a versatile
Junyan Zhang et al.
Electrophoresis, 40(7), 1034-1040 (2018-12-24)
Electrokinetic motion of a micro oil droplet beneath a glass slide was experimentally investigated in this paper. The micro oil droplets were released under the glass slide in an aqueous solution and the motion along the glass slide was measured
Alexander Zarkov et al.
Biotechnology, biotechnological equipment, 28(1), 112-117 (2014-01-02)
Here we propose an easy to build up and apply glass slide preparation system for single DNA molecules stretching. It is based on fast and simple coating of a solid glass with a cocktail of acrylic monomers that are easily
Sen Xiang et al.
The Analyst, 137(7), 1669-1673 (2012-02-16)
In this work, two different deposition methods of 3-aminopropyltriethoxysilane (APTES) on glass slides were compared in order to study the adhesion effect of cervical exfoliated cells on smear slides. Glass slides were modified by vapor-phase deposition (V-D) and liquid-phase deposition
A V Vasiliskov et al.
BioTechniques, 27(3), 592-594 (1999-09-18)
The manufacturing of microchips containing oligonucleotides and proteins immobilized within gel pads, ranging in size from 10 x 10 to 100 x 100 microns, is described. The microchips are produced by photo- or persulfate-induced copolymerization of unsaturated derivatives of biomolecules
Mitsuru Yasuda et al.
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 28(10), 947-952 (2012-10-13)
This paper presents highly sensitive fluorescence detections of avidin and streptavidin using an optical interference mirror (OIM) slide consisting of a plane mirror covered with an optical interference layer. Compared with a common glass slide, the OIM slide can enhance
Taiga Uekusa et al.
Journal of pharmaceutical and biomedical analysis, 161, 45-50 (2018-08-26)
The purpose of the present study was to investigate the precipitation of a drug on the particle surface of its salt in biorelevant media. Pioglitazone (PIO, weak base, pKa = 5.8) was used as a model drug. The crystal particles of PIO
Emmanuel I Odongo-Aginya et al.
African health sciences, 7(1), 33-36 (2007-07-03)
The Kato-Katz thick smear technique is the standard technique recommended by the World Health Organisation for the quantitative diagnosis of Schistosoma mansoni and other intestinal helminth infections. The major problem of the technique is that a few hours after the
Barbara I Fall et al.
Analytical chemistry, 75(3), 556-562 (2003-02-15)
The described in vitro test system for allergy diagnosis is based on microscope glass slides activated with (3-glycidyloxypropyl)trimethoxysilane. Allergen solutions are immobilized as small droplets (approximately 10 nL) on the activated glass slides with a piezoelectric arrayer. In contrast to
Zelal Adiguzel et al.
Materials science & engineering. C, Materials for biological applications, 78, 942-948 (2017-06-04)
This work describes a soft lithographic method for the generation of patterned both biopolymer and silver with each covered on microscope glass. Because of their biocompatible nature and permeability to gases the biopolymers are convenient for cell culture studies. The
Ted Pham et al.
Methods in molecular biology (Clifton, N.J.), 1264, 35-46 (2015-01-30)
Single mitochondrial assays are uncovering a new level of biological heterogeneity, holding promises for a better understanding of molecular respiration and mitochondria-related diseases. Here, we present a nanoscale approach to trapping single mitochondria in fluidic channels for fluorescence microscopy. We
Victoria Fox
Methods in molecular biology (Clifton, N.J.), 767, 161-173 (2011-08-09)
For certain applications, particularly experiments involving high-resolution imaging, it is necessary to culture cells on glass slides or cover glasses. This chapter describes techniques for successfully growing human embryonic stem cells (hESCs) on glass surfaces under three different conditions -
Vianney Souplet et al.
Methods in molecular biology (Clifton, N.J.), 570, 287-297 (2009-08-04)
Microarrays are frequently prepared on microscope glass slides. However, glass substrates can break or cut and thus can lead to the contamination of the manipulator during the analysis of biological samples. Alternately, bisphenol A polycarbonate (PC) is shock-resistant and, in
Satoshi Yagi et al.
Dental materials journal, 30(5), 611-615 (2011-09-29)
In this study, different glasses were investigated to improve reusable investments. Borosilicate glass (BSG) powder and soda-lime glass (SLG) powder were prepared by milling broken beakers and microscope slides, respectively, and used in experimental investments (I-BSG, I-SLG) by blending glass
G Danscher et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 31(12), 1394-1398 (1983-12-01)
RNase labeled with colloidal gold was used as a model for the present technique evolved for the light microscopic localization of gold-labeled substances in semithin resin-embedded sections. Tissue sections placed on glass slides were treated with the gold-enzyme complex and
Barna Kovács et al.
Biosensors & bioelectronics, 18(2-3), 111-118 (2002-12-18)
An optical biosensor for urea measurements was developed. The operation of the sensor is based on the well-known urease enzyme-catalyzed hydrolysis of urea. The ammonium ions liberated in the reaction are detected with an ion selective optode membrane containing nonactin
J L Perry et al.
Journal of clinical microbiology, 27(6), 1411-1412 (1989-06-01)
An opaque, water-insoluble quality control material with a skinlike microscopic appearance was prepared by inoculating melted xanthine (0.4%) agar with filamentous fungi and dispensing drops onto glass slides. After solidification of the agar, the material was rapidly cleared by 10%
Duy Khiem Nguyen et al.
Analytical biochemistry, 605, 113807-113807 (2020-06-12)
We present a label-free liquid crystal-based biosensor for the detection of dopamine (DA) in aqueous solutions using dopamine-binding aptamers (DBA) as recognition elements. In this system, the dimethyloctadecyl [3-(trimethoxysilyl) propyl] ammonium chloride (DMOAP) self-assembled monolayers immobilized on glass slides support
Marcia B C Mello et al.
Malaria journal, 13, 238-238 (2014-06-19)
Due to students' initial inexperience, slides are frequently broken and blood smears are damaged in microscopy training, leading to the need for their constant replacement. To minimize this problem a method of preparing blood smears on transparent acetate sheets was
Ana V Almeida et al.
Lab on a chip, 16(6), 1063-1071 (2016-02-26)
We demonstrate the use of open-surface microfluidics to sequence DNA by pyrosequencing at the plain hydrophobically coated surface of a microscope glass cover slip. This method offers significant advantages in terms of instrument size, simplicity, disposability, and functional integration, particularly
Paola Bonsi et al.
Alternatives to laboratory animals : ATLA, 30(2), 241-247 (2002-04-25)
Two experimental models suitable for the screening of volatile compounds were set up. The first consisted of a glass-chamber slide with eight wells, one carrying the test compound, and the others carrying cells in monolayers. In the second model, the
Ping Zhu et al.
Annals of vascular surgery, 26(2), 268-275 (2012-02-07)
To investigate the effects of matrine on the vascular smooth muscle cell (VSMC) migration modulated by disturbed flow and their underlying molecular mechanisms in vitro. Isolated rat aortic VSMCs were grown to confluence on 20- × 80-mm fibronectin-coated glass cover
Rémi Desmet et al.
Methods in molecular biology (Clifton, N.J.), 669, 135-145 (2010-09-22)
Peptide microarrays are useful tools for characterizing the humoral response against methylated antigens. They are usually prepared by printing unmodified and methylated peptides on substrates such as functionalized microscope glass slides. The preferential capture of antibodies by methylated peptides suggests
Jürgen J Schmied et al.
Nature protocols, 9(6), 1367-1391 (2014-05-17)
Validating and testing a fluorescence microscope or a microscopy method requires defined samples that can be used as standards. DNA origami is a new tool that provides a framework to place defined numbers of small molecules such as fluorescent dyes
Camille Cuveillier et al.
Bio-protocol, 11(7), e3968-e3968 (2021-04-24)
Microtubules (MT) are the most rigid component of the cytoskeleton. Nevertheless, they often appear highly curved in the cellular context and the mechanisms governing their overall shape are poorly understood. Currently, in vitro microtubule analysis relies primarily on electron microscopy
Nagaraju Chada et al.
Scientific reports, 5, 12550-12550 (2015-08-01)
Though ubiquitous in optical microscopy, glass has long been overlooked as a specimen supporting surface for high resolution atomic force microscopy (AFM) investigations due to its roughness. Using bacteriorhodopsin from Halobacterium salinarum and the translocon SecYEG from Escherichia coli, we
Kei Takahashi et al.
Journal of biological engineering, 9, 3-3 (2015-05-15)
The cytosol of amoeba cells controls the membrane deformation during their motion in vivo. To investigate such ability of the cytosol of amoeba cell, Dictyostelium discoideum (Dictyostelium), in vitro, we used lipids extracted from Dictyostelium and commercially available phospholipids, and
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