D2629

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In situ activities of hexokinase and fructokinase in relation to phosphorylation status of root meristem cells of Vicia faba during reactivation from sugar starvation.

Justyna T Polit et al.

Physiologia plantarum, 135(4), 342-350 (2009-04-02)

The plant cell cycle is equipped with two principal control points: PCP1 in G1 and PCP2 in G2 phase. These checkpoints can arrest the cell cycle in response to carbohydrate starvation, while sugar presence can revive the replication and mitotic

Tiger, Bengal and Domestic Cat Embryos Produced by Homospecific and Interspecific Zona-Free Nuclear Transfer.

L N Moro et al.

Reproduction in domestic animals = Zuchthygiene, 50(5), 849-857 (2015-09-04)

The aim of this study was to evaluate three different cloning strategies in the domestic cat (Felis silvestris) and to use the most efficient to generate wild felid embryos by interspecific cloning (iSCNT) using Bengal (a hybrid formed by the

Postovulatory ageing modifies sperm-induced Ca2+ oscillations in mouse oocytes through a conditions-dependent, multi-pathway mechanism.

Marcin Szpila et al.

Scientific reports, 9(1), 11859-11859 (2019-08-16)

Postovulatory ageing of mammalian oocytes occurs between their ovulation and fertilization and has been shown to decrease their developmental capabilities. Aged oocytes display numerous abnormalities, including altered Ca2+ signalling. Fertilization-induced Ca2+ oscillations are essential for activation of the embryonic development

Optimizing the conditions for in vitro maturation and artificial activation of sika deer (Cervus nippon hortulorum) oocytes.

Y Yin et al.

Reproduction in domestic animals = Zuchthygiene, 48(1), 27-32 (2012-03-31)

With the goal of establishing experimental protocols for cloning sika deer, various conditions for in vitro maturation (IVM) and artificial activation of sika deer oocytes were examined. In vitro maturation was evaluated in seven different culture media. The highest rate

Horse ooplasm supports in vitro preimplantation development of zebra ICSI and SCNT embryos without compromising YAP1 and SOX2 expression pattern.

Andrés Gambini et al.

PloS one, 15(9), e0238948-e0238948 (2020-09-12)

Several equids have gone extinct and many extant equids are currently considered vulnerable to critically endangered. This work aimed to evaluate whether domestic horse oocytes support preimplantation development of zebra embryos obtained by intracytoplasmic sperm injection (ICSI, zebroid) and cloning

microRNA-670 modulates Igf2bp1 expression to regulate RNA methylation in parthenogenetic mouse embryonic development.

Jindong Hao et al.

Scientific reports, 10(1), 4782-4782 (2020-03-18)

Aberrant epigenetic modification, including N6-methylation of adenosine (m6A), has been frequently reported in embryos derived from parthenogenetic activation (PA). However, the role of Igf2bp1 expression pattern in m6A modification and the mechanism through which Igf2bp1 function is regulated in PA

Factors Affecting Mouse Somatic Cell Nuclear Reprogramming by Rabbit Ooplasms.

Xia Huang et al.

Cellular reprogramming, 19(6), 344-353 (2017-11-15)

Successful development of interspecies somatic cell nuclear transfer (iSCNT) embryos depends on compatibilities between ooplasmic and nuclear components. However, the mechanisms by which the compatibilities are regulated are still unknown. In this study, using mouse Oct4-green fluorescent protein (GFP) cells

Pregnancy and Calving Rates of Cloned Dromedary Camels Produced by Conventional and Handmade Cloning Techniques and In Vitro and In Vivo Matured Oocytes.

F Moulavi et al.

Molecular biotechnology, 62(9), 433-442 (2020-07-16)

Despite practical implication of cloning in camelids, its broad application has been hampered by technical and biological problems. Method of somatic cell nuclear transfer (SCNT) and oocyte competence are the principal technical and biological factors, respectively, that determine the cloning

Differential effects of 6-DMAP, olomoucine and roscovitine on Xenopus oocytes and eggs.

S Flament et al.

Zygote (Cambridge, England), 8(1), 3-14 (2000-06-07)

The effects of the new cyclin-dependent kinase inhibitors, roscovitine and olomoucine, on oocytes and eggs of Xenopus laevis were investigated and compared with those of 6-dimethylamino purine (6-DMAP). The inhibitory properties of 6-DMAP, olomoucine and roscovitine towards p34cdc2-cyclin B isolated

Epigenetic regulation of the bacterial cell cycle.

Justine Collier

Current opinion in microbiology, 12(6), 722-729 (2009-09-29)

N(6)-methyl-adenines can serve as epigenetic signals for interactions between regulatory DNA sequences and regulatory proteins that control cellular functions, such as the initiation of chromosome replication or the expression of specific genes. Several of these genes encode master regulators of

Short-term treatment with 6-DMAP and demecolcine improves developmental competence of electrically or Thi/DTT-activated porcine parthenogenetic embryos.

Sol Ji Park et al.

Zygote (Cambridge, England), 19(1), 1-8 (2010-06-24)

Treatment with 6-dimethylaminopurine (6-DMAP) or demecolcine (DE) for several (at least 2) hours after artificial activation is known to improve in vitro development of porcine embryos. However, several reports have also shown that treatments with these chemicals induce apoptosis. The

Expression profiles and function analysis of microRNAs in postovulatory aging mouse oocytes.

Tian-Yang Wang et al.

Aging, 9(4), 1186-1201 (2017-04-11)

In this study, microRNA (miRNA) profiles in postovulatory aging mouse oocytes were analyzed by microarray screening and RT-qPCR. Hierarchical cluster analysis on the microarray data and KEGG pathway enrichment analysis on the mRNAs targeted by differentially expressed (DE) miRNAs between

Developmental potential of human oocytes matured in vitro followed by vitrification and activation.

Patrick Imesch et al.

Journal of ovarian research, 6, 30-30 (2013-04-20)

Oocyte in vitro maturation (IVM) and cryopreservation at the time of routine ovarian tissue freezing may be offered to cancer patients as an additional option for fertility preservation. This study aimed to investigate the developmental capacity of oocytes isolated from

Meiotic block with roscovitine improves competence of porcine oocytes by fine-tuning activities of different cyclin-dependent kinases.

Shuai Zhu et al.

Journal of cellular physiology, 235(10), 7530-7540 (2020-02-27)

Successful use of oocytes from small follicles (SFs) is of great importance for animal embryo production and human in vitro fertilization with reduced hormone-related side effects. How in vitro meiotic arrest maintenance (MAM) increases the competence of oocytes is not

Overexpression of OCT4A ortholog elevates endogenous XIST in porcine parthenogenic blastocysts.

Jae Yeon Hwang et al.

The Journal of reproduction and development, 61(6), 533-540 (2015-08-11)

X-chromosome inactivation (XCI) is an epigenetic process that equalizes expression of X-borne genes between male and female eutherians. This process is observed in early eutherian embryo development in a species-specific manner. Until recently, various pluripotent factors have been suggested to

Increasing CRISPR/Cas9-mediated homology-directed DNA repair by histone deacetylase inhibitors.

Guoling Li et al.

The international journal of biochemistry & cell biology, 125, 105790-105790 (2020-06-14)

Histone deacetylase inhibitors (HDACis) affect DNA repair pathways by modulating multiple cellular machineries, including chromatin state, DNA repair factor modification, and the cell cycle. These machineries can differentially affect DNA repair outcomes. With the aim to investigate the impacts of

Effect of cyclosporine pretreatment on mitochondrial function in vitrified bovine mature oocytes.

Xue-Ming Zhao et al.

Fertility and sterility, 95(8), 2786-2788 (2011-06-07)

Vitrification had a significantly negative impact on the mitochondrial function of bovine oocytes. However, 40 μg/mL cyclosporine pretreatment before vitrification contributed greatly to maintaining mitochondrial membrane potential and adenosine triphosphate content, decreasing reactive oxygen species level, and thereby increasing the

Germline transgenic pigs by Sleeping Beauty transposition in porcine zygotes and targeted integration in the pig genome.

Wiebke Garrels et al.

PloS one, 6(8), e23573-e23573 (2011-09-08)

Genetic engineering can expand the utility of pigs for modeling human diseases, and for developing advanced therapeutic approaches. However, the inefficient production of transgenic pigs represents a technological bottleneck. Here, we assessed the hyperactive Sleeping Beauty (SB100X) transposon system for

Dual fluorescence and ultrafast intramolecular charge transfer with 6-N,N-dialkylaminopurines. A two-state model.

Attila Demeter et al.

The journal of physical chemistry. A, 115(9), 1521-1537 (2011-02-15)

6-N,N-Dimethyl-9-methyladenine (DMPURM) and 6-N,N-dimethyladenine (DMPURH) show dual fluorescence from a locally excited (LE) and an intramolecular charge transfer (ICT) state in solvents of different polarity over extended temperature ranges. The fluorescence quantum yields are very small, in particular those of

Evidence for conserved DNA and histone H3 methylation reprogramming in mouse, bovine and rabbit zygotes.

Konstantin Lepikhov et al.

Epigenetics & chromatin, 1(1), 8-8 (2008-11-19)

In mammals the parental genomes are epigenetically reprogrammed after fertilization. This reprogramming includes a rapid demethylation of the paternal (sperm-derived) chromosomes prior to DNA replication in zygotes. Such active DNA demethylation in the zygote has been documented for several mammalian

Improved embryo development using high cysteamine concentration during IVM and sperm co-culture with COCs previous to ICSI in bovine.

Natalia Gabriela Canel et al.

Theriogenology, 117, 26-33 (2018-05-29)

In contrast to other species, intracytoplasmic sperm injection (ICSI) in bovine remains inefficient, resulting in low embryo developmental rates. It is unclear whether such inefficiency is due to the poor response of bovine ooplasms to the injection stimulus, or to

Slow controlled-rate freezing of human in vitro matured oocytes: effects on maturation rate and kinetics and parthenogenetic activation.

Karen Versieren et al.

Fertility and sterility, 96(3), 624-628 (2011-07-22)

To create a pool of frozen donated human oocytes and find the optimal stage for slow controlled-rate freezing of human in vitro matured oocytes. Oocytes at different developmental stages of maturation (germinal vesicle, metaphase I, or metaphase II) and oocytes that

Effects of different activation regimens on pronuclear formation and developmental competence of in vitro-matured porcine oocytes after intracytoplasmic sperm injection.

W-M Cheng et al.

Reproduction in domestic animals = Zuchthygiene, 47(4), 609-614 (2011-11-02)

To improve the activation protocol for in vitro-maturated porcine oocytes after intracytoplasmic sperm injection (ICSI), we examined the combined effect of U0126, a specific inhibitor of mitogen-activated protein kinase kinases, and an electrical pulse on pronuclear formation and developmental competence.

A combined treatment with ethanol and 6-dimethylaminopurine is effective for the activation and further embryonic development of oocytes from Sprague-Dawley and Wistar rats.

Daisuke Sano et al.

Zygote (Cambridge, England), 17(1), 29-36 (2008-10-18)

In nuclear-transferred or round spermatid-injected oocytes, artificial activation is required for further development in mammals. Although strontium chloride is widely used as the reagent for inducing oocyte activation in mice, the optimal method for oocyte activation remains controversial in rats

Changes of spontaneous parthenogenetic activation and development potential of golden hamster oocytes during the aging process.

Han Jiang et al.

Acta histochemica, 117(1), 104-110 (2014-12-07)

The golden hamster is an excellent animal experimental model for oocyte research. The hamster oocytes are very useful in clinical examination of human spermatozoan activity. Non-fertile oocytes can lead to time-dependent processes of aging, which will affect the results of

Efficient activation of reconstructed rat embryos by cyclin-dependent kinase inhibitors.

Robin L Webb et al.

PloS one, 5(3), e9799-e9799 (2010-03-25)

Over the last decade a number of species, from farm animals to rodents, have been cloned using somatic cell nuclear transfer technology (SCNT). This technique has the potential to revolutionize the way that genetically modified animals are made. In its

Generation of myostatin edited horse embryos using CRISPR/Cas9 technology and somatic cell nuclear transfer.

Lucia Natalia Moro et al.

Scientific reports, 10(1), 15587-15587 (2020-09-26)

The application of new technologies for gene editing in horses may allow the generation of improved sportive individuals. Here, we aimed to knock out the myostatin gene (MSTN), a negative regulator of muscle mass development, using CRISPR/Cas9 and to generate

Calcium Oscillatory Patterns and Oocyte Activation During Fertilization: a Possible Mechanism for Total Fertilization Failure (TFF) in Human In Vitro Fertilization?

Bei Sun et al.

Reproductive sciences (Thousand Oaks, Calif.), 28(3), 639-648 (2020-08-20)

This paper reviews the effects of calcium oscillatory patterns in oocytes and early embryo development. Total fertilization failure (TFF) is the failure of fertilization in all oocytes in a human IVF cycle, even after treatment with intracytoplasmic sperm injection (ICSI).

Amyloid-like substance in mice and human oocytes and embryos.

Ricardo N Pimentel et al.

Journal of assisted reproduction and genetics, 36(9), 1877-1890 (2019-07-25)

To identify and characterize amyloid-like substance (ALS) in human and mouse oocytes and preimplantation embryos. An experimental prospective pilot study. A total of 252 mouse oocytes and preimplantation embryos and 50 immature and in vitro matured human oocytes and parthenogenetic

Effect of cycloheximide, 6-DMAP, roscovitine and butyrolactone I on resumption of meiosis in porcine oocytes.

Gaid Le Beux et al.

Theriogenology, 60(6), 1049-1058 (2003-08-26)

Improvement of the ability to maintain germinal vesicle stage oocytes in vitro is important for the acquisition of developmental competence. Maintaining oocytes at this stage without damaging their quality would allow synchronization of maturation and homogenization of the oocytes population.

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