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Kumiko Takeda et al.
The Journal of reproduction and development, 58(3), 323-329 (2012-03-27)
Interspecies/intergeneric mitochondrial heteroplasmy can occur in interspecies/intergeneric hybrid embryos or following nuclear transfer. In the present study, intergeneric buffalo (Bubalus bubalis) mitochondria (WB-mt) or interspecies murine (Mus spretus) mitochondria (M-mt) were injected into bovine (Bos taurus) oocytes, and the subsequent
Konstantin Lepikhov et al.
Epigenetics & chromatin, 1(1), 8-8 (2008-11-19)
In mammals the parental genomes are epigenetically reprogrammed after fertilization. This reprogramming includes a rapid demethylation of the paternal (sperm-derived) chromosomes prior to DNA replication in zygotes. Such active DNA demethylation in the zygote has been documented for several mammalian
Natalia Gabriela Canel et al.
Theriogenology, 117, 26-33 (2018-05-29)
In contrast to other species, intracytoplasmic sperm injection (ICSI) in bovine remains inefficient, resulting in low embryo developmental rates. It is unclear whether such inefficiency is due to the poor response of bovine ooplasms to the injection stimulus, or to
Zhe Han et al.
Frontiers in cell and developmental biology, 9, 609311-609311 (2021-03-16)
As a major protein of the polyhedral coat of coated pits and vesicles, clathrin molecules have been shown to play a stabilization role for kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridges. Clathrin heavy chain 1 (CLTC)
Lucia Natalia Moro et al.
Scientific reports, 10(1), 15587-15587 (2020-09-26)
The application of new technologies for gene editing in horses may allow the generation of improved sportive individuals. Here, we aimed to knock out the myostatin gene (MSTN), a negative regulator of muscle mass development, using CRISPR/Cas9 and to generate
De Cheng et al.
PloS one, 7(12), e51778-e51778 (2012-12-20)
Porcine induced pluripotent stem (piPS) cell lines have been generated recently by using a cocktail of defined transcription factors, however, the features of authentic piPS cells have not been agreed upon and most of published iPS clones did not meet
Justyna Teresa Polit et al.
Journal of plant physiology, 169(16), 1597-1606 (2012-07-10)
Carbohydrate starvation of Vicia faba root meristems leads to readjustment of carbohydrate metabolism and blocks the cell cycle in two principal control points (PCP1/2). The cell cycle reactivation is possible after sucrose provision, although with a delay of about 12h.
Robin L Webb et al.
PloS one, 5(3), e9799-e9799 (2010-03-25)
Over the last decade a number of species, from farm animals to rodents, have been cloned using somatic cell nuclear transfer technology (SCNT). This technique has the potential to revolutionize the way that genetically modified animals are made. In its
Karen Versieren et al.
Fertility and sterility, 96(3), 624-628 (2011-07-22)
To create a pool of frozen donated human oocytes and find the optimal stage for slow controlled-rate freezing of human in vitro matured oocytes. Oocytes at different developmental stages of maturation (germinal vesicle, metaphase I, or metaphase II) and oocytes that
W-M Cheng et al.
Reproduction in domestic animals = Zuchthygiene, 47(4), 609-614 (2011-11-02)
To improve the activation protocol for in vitro-maturated porcine oocytes after intracytoplasmic sperm injection (ICSI), we examined the combined effect of U0126, a specific inhibitor of mitogen-activated protein kinase kinases, and an electrical pulse on pronuclear formation and developmental competence.
Oleg Fedorov et al.
Proceedings of the National Academy of Sciences of the United States of America, 104(51), 20523-20528 (2007-12-14)
Protein kinases play a pivotal role in cell signaling, and dysregulation of many kinases has been linked to disease development. A large number of kinase inhibitors are therefore currently under investigation in clinical trials, and so far seven inhibitors have
Bei Sun et al.
Reproductive sciences (Thousand Oaks, Calif.), 28(3), 639-648 (2020-08-20)
This paper reviews the effects of calcium oscillatory patterns in oocytes and early embryo development. Total fertilization failure (TFF) is the failure of fertilization in all oocytes in a human IVF cycle, even after treatment with intracytoplasmic sperm injection (ICSI).
WooJae Choi et al.
Zygote (Cambridge, England), 23(6), 916-923 (2014-11-27)
Genome-editing technologies are considered to be an important tool for generating gene knockout cattle models. Here, we report highly efficient disruption of a chromosomally integrated eGFP gene in bovine somatic cells using RNA-guided endonucleases, a new class of programmable nucleases
Ricardo N Pimentel et al.
Journal of assisted reproduction and genetics, 36(9), 1877-1890 (2019-07-25)
To identify and characterize amyloid-like substance (ALS) in human and mouse oocytes and preimplantation embryos. An experimental prospective pilot study. A total of 252 mouse oocytes and preimplantation embryos and 50 immature and in vitro matured human oocytes and parthenogenetic
Anna T Grazul-Bilska et al.
Reproduction (Cambridge, England), 147(5), 639-648 (2014-01-30)
Utero-placental growth and vascular development are critical for pregnancy establishment that may be altered by various factors including assisted reproductive technologies (ART), nutrition, or others, leading to compromised pregnancy. We hypothesized that placental vascularization and expression of angiogenic factors are
Gaid Le Beux et al.
Theriogenology, 60(6), 1049-1058 (2003-08-26)
Improvement of the ability to maintain germinal vesicle stage oocytes in vitro is important for the acquisition of developmental competence. Maintaining oocytes at this stage without damaging their quality would allow synchronization of maturation and homogenization of the oocytes population.
Y Yin et al.
Reproduction in domestic animals = Zuchthygiene, 48(1), 27-32 (2012-03-31)
With the goal of establishing experimental protocols for cloning sika deer, various conditions for in vitro maturation (IVM) and artificial activation of sika deer oocytes were examined. In vitro maturation was evaluated in seven different culture media. The highest rate
Brahmasani Sambasiva Rao et al.
The Journal of reproduction and development, 56(6), 623-629 (2010-08-17)
The ability to rescue gametes from endangered or wildlife species and to subsequently produce viable embryos holds tremendous potential as a means to increase the population size of endangered or wildlife species. The objective of this study was to assess
Daisuke Sano et al.
Zygote (Cambridge, England), 17(1), 29-36 (2008-10-18)
In nuclear-transferred or round spermatid-injected oocytes, artificial activation is required for further development in mammals. Although strontium chloride is widely used as the reagent for inducing oocyte activation in mice, the optimal method for oocyte activation remains controversial in rats
Han Jiang et al.
Acta histochemica, 117(1), 104-110 (2014-12-07)
The golden hamster is an excellent animal experimental model for oocyte research. The hamster oocytes are very useful in clinical examination of human spermatozoan activity. Non-fertile oocytes can lead to time-dependent processes of aging, which will affect the results of
Daekee Kwon et al.
Primates; journal of primatology, 57(4), 471-478 (2016-05-12)
Monkey interorder somatic cell nuclear transfer (iSCNT) using enucleated cow oocytes yielded poor blastocysts development and contradictory results among research groups. Determining the reason for this low blastocyst development is a prerequisite for optimizing iSCNT in rhesus monkeys. The aim
Attila Demeter et al.
The journal of physical chemistry. A, 115(9), 1521-1537 (2011-02-15)
6-N,N-Dimethyl-9-methyladenine (DMPURM) and 6-N,N-dimethyladenine (DMPURH) show dual fluorescence from a locally excited (LE) and an intramolecular charge transfer (ICT) state in solvents of different polarity over extended temperature ranges. The fluorescence quantum yields are very small, in particular those of
L N Moro et al.
Reproduction (Cambridge, England), 150(1), 1-10 (2015-03-31)
The aim of this study was to evaluate the capacity of domestic cat (Dc, Felis silvestris) oocytes to reprogram the nucleus of cheetah (Ch, Acinonyx jubatus) cells by interspecies SCNT (iSCNT), by using embryo aggregation. Dc oocytes were in vitro
Shuai Zhu et al.
Journal of cellular physiology, 235(10), 7530-7540 (2020-02-27)
Successful use of oocytes from small follicles (SFs) is of great importance for animal embryo production and human in vitro fertilization with reduced hormone-related side effects. How in vitro meiotic arrest maintenance (MAM) increases the competence of oocytes is not
Guoling Li et al.
The international journal of biochemistry & cell biology, 125, 105790-105790 (2020-06-14)
Histone deacetylase inhibitors (HDACis) affect DNA repair pathways by modulating multiple cellular machineries, including chromatin state, DNA repair factor modification, and the cell cycle. These machineries can differentially affect DNA repair outcomes. With the aim to investigate the impacts of
Shun Zhang et al.
Molecular biology reports, 46(1), 251-259 (2018-11-12)
The binding of exogenous DNA to sperm is a key process for sperm-mediated gene transfer; however, the underlying molecular mechanisms have yet to be elucidated. In the present study, we aimed to identify the DNA binding proteins (DBPs) in rabbit
Maria Valéria de Oliveira Santos et al.
Theriogenology, 128, 74-80 (2019-02-12)
The use of natural antioxidants in culture media can be an alternative to minimize the negative effects of oxidative stress produced by culture conditions. Essential oil from Syzygium aromaticum (EOSA) has therapeutic properties, including antioxidant activity in different cell types
Tian-Yang Wang et al.
Aging, 9(4), 1186-1201 (2017-04-11)
In this study, microRNA (miRNA) profiles in postovulatory aging mouse oocytes were analyzed by microarray screening and RT-qPCR. Hierarchical cluster analysis on the microarray data and KEGG pathway enrichment analysis on the mRNAs targeted by differentially expressed (DE) miRNAs between
Xia Huang et al.
Cellular reprogramming, 19(6), 344-353 (2017-11-15)
Successful development of interspecies somatic cell nuclear transfer (iSCNT) embryos depends on compatibilities between ooplasmic and nuclear components. However, the mechanisms by which the compatibilities are regulated are still unknown. In this study, using mouse Oct4-green fluorescent protein (GFP) cells
F Moulavi et al.
Molecular biotechnology, 62(9), 433-442 (2020-07-16)
Despite practical implication of cloning in camelids, its broad application has been hampered by technical and biological problems. Method of somatic cell nuclear transfer (SCNT) and oocyte competence are the principal technical and biological factors, respectively, that determine the cloning
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