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H A Goldberg et al.
Analytical biochemistry, 251(2), 227-233 (1997-09-23)
A number of acidic proteins, such as those found in bone and dentin, are poorly resolved on acrylamide gels using Coomassie blue or silver nitrate staining. The cationic dye Stains-all allows visualization and identification of these proteins due to their
Chromatin and Chromosomal Protein Research III (1978)
Santosh Kumar Sahu et al.
Biotechnology letters, 30(12), 2131-2137 (2008-07-17)
Human phospholipid scramblase 1 (hPLSCR1) scrambles plasma membrane phospholipids during cell activation, blood coagulation and apoptosis. It was over-expressed in E. coli with a histidine tag and purified from the inclusion bodies (*30 mg/l culture broth) under denaturing conditions using
Anu S Maharjan et al.
PloS one, 6(10), e26078-e26078 (2011-10-25)
Following tissue injury, monocytes can enter the tissue and differentiate into fibroblast-like cells called fibrocytes, but little is known about what regulates this differentiation. Extracellular matrix contains high molecular weight hyaluronic acid (HMWHA; ∼2×10(6) Da). During injury, HMWHA breaks down
Y Sharma et al.
FEBS letters, 326(1-3), 59-64 (1993-07-12)
We show that the calcium-mimic dye, Stains-all, is a convenient probe to study the structural features of the individual calcium-binding sites of calmodulin (CaM) and related calcium-binding proteins (CaBP). These peptides bind the dye in their calcium-binding sites, and induce
Nikita A Kuznetsov et al.
PloS one, 9(6), e98495-e98495 (2014-06-10)
Human 8-oxoguanine DNA glycosylase (hOGG1) is a key enzyme responsible for initiating the base excision repair of 7,8-dihydro-8-oxoguanosine (oxoG). In this study a thermodynamic analysis of the interaction of hOGG1 with specific and non-specific DNA-substrates is performed based on stopped-flow
Shigeki Suzuki et al.
PloS one, 9(11), e112490-e112490 (2014-11-15)
Phosphophoryn (PP) is generated from the proteolytic cleavage of dentin sialophosphoprotein (DSPP). Gene duplications in the ancestor dentin matrix protein-1 (DMP-1) genomic sequence created the DSPP gene in toothed animals. PP and DMP-1 are phosphorylated extracellular matrix proteins that belong
K A Homer et al.
Analytical biochemistry, 214(2), 435-441 (1993-11-01)
Undegraded glycosaminoglycans form a complex with 1-ethyl-2-[3-(1-ethylnaphtho-[1,2-d]thiazolin-2-ylidene)-2- methylpropenyl]naphtho-[1,2-d]thiazolium bromide (Stains-all) in solution resulting in a characteristic shift in spectrum. Hyaluronic acid (a nonsulfated glycosaminoglycan) reacts with the dye to form a complex with an absorbance maximum at 650 nm, while
Jiqin Wu et al.
Journal of virology, 89(1), 249-261 (2014-10-17)
The flavivirus NS5 is a natural fusion of a methyltransferase (MTase) and an RNA-dependent RNA polymerase (RdRP). Analogous to DNA-dependent RNA polymerases, the NS5 polymerase initiates RNA synthesis through a de novo mechanism and then makes a transition to a
Petra Školáková et al.
Nucleic acids research, 43(9), 4733-4745 (2015-04-10)
There are two basic mechanisms that are associated with the maintenance of the telomere length, which endows cancer cells with unlimited proliferative potential. One mechanism, referred to as alternative lengthening of telomeres (ALT), accounts for approximately 10-15% of all human
Olwen Domingo et al.
Nucleic acids research, 43(11), 5275-5283 (2015-05-03)
Propargyl groups are attractive functional groups for labeling purposes, as they allow CuAAC-mediated bioconjugation. Their size minimally exceeds that of a methyl group, the latter being frequent in natural nucleotide modifications. To understand under which circumstances propargyl-containing oligodeoxynucleotides preserve base
H Ksiezak-Reding et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 7(11), 3554-3560 (1987-11-01)
The phosphorylation state of human and bovine spinal cord neurofilaments (NF) was studied by direct phosphate analysis and carbocyanine dye ("Stains-all") binding to NF polypeptides resolved on SDS-polyacrylamide gels. Electrophoretically purified NF-H (200 kDa), NF-M (160 kDa), and NF-L (68
Ryuji Nomura et al.
Histochemistry and cell biology, 135(6), 531-538 (2011-05-19)
Calreticulin (CRT)-1 is a major Ca(2+)-buffering protein in the lumen of the endoplasmic reticulum. Human and murine CRT-2 was isolated in 2002, but the subcellular localization and function is still unclear. Here, we studied the intracellular localization and function of
B W Moore
Neurochemical research, 13(6), 539-545 (1988-06-01)
The binding of Ca2+ to rat or bovine S-100 proteins, in the absence of ligands, showed a dissociation constant (in 60 mM K+) of 0.5 to 1.0 mM as measured by the effects of Ca2+ on binding of S-100 to
Joshua D Brown et al.
Science (New York, N.Y.), 368(6489), 413-417 (2020-04-25)
Heterogeneous transcriptional start site usage by HIV-1 produces 5'-capped RNAs beginning with one, two, or three 5'-guanosines (Cap1G, Cap2G, or Cap3G, respectively) that are either selected for packaging as genomes (Cap1G) or retained in cells as translatable messenger RNAs (mRNAs)
Marta Krystyna Kosinska et al.
PloS one, 10(5), e0125192-e0125192 (2015-05-02)
Hyaluronic acid (HA), lubricin, and phospholipid species (PLs) contribute independently or together to the boundary lubrication of articular joints that is provided by synovial fluid (SF). Our study is the first reporting quantitative data about the molecular weight (MW) forms
D Mahadevan et al.
Biochemistry, 33(3), 746-754 (1994-01-25)
Src-homology region 2 (SH2) domains are stretches of about 100 amino acids which are found to be structurally conserved in a number of signaling molecules. These regions have been shown to bind with high affinity to phosphotyrosine residues within activated
Anahita Shahraz et al.
Scientific reports, 5, 16800-16800 (2015-11-20)
Oligosialic and polysialic acid (oligoSia and polySia) of the glycocalyx of neural and immune cells are linear chains, in which the sialic acid monomers are α2.8-glycosidically linked. Sialic acid-binding immunoglobulin-like lectin-11 (SIGLEC-11) is a primate-lineage specific receptor of human tissue
Nana Yamada et al.
Protoplasma, 252(6), 1519-1527 (2015-03-07)
The cytoplasmic free Ca(2+) could play an important role for salt tolerance in rice root (Oryza sativa L.). Here, we compared the expression profiles of two putative developmentally regulated plasma membrane polypeptides (DREPP1 and DREPP2) in rice roots of salt-tolerant
A method for enhancing the sensitivity and stability of stains-all for phosphoproteins separated in sodium dodecyl sulfate-polyacrylamide gels.
J M Myers et al.
Analytical biochemistry, 240(2), 300-302 (1996-09-05)
H G Lee et al.
Analytical biochemistry, 219(2), 278-287 (1994-06-01)
An electrophoretic method is described for determining the molecular weight distribution of hyaluronan (HA). The method involves separation of HA by electrophoresis on a 0.5% agarose gel, followed by detection of HA using the cationic dye Stains-All (3,3'-dimethyl-9-methyl-4,5,4'5'-dibenzothiacarbocyanine). The recommended
Anat Marom et al.
Advanced healthcare materials, 4(10), 1478-1483 (2015-05-09)
The study introduces a "brain-on-a-chip" microfluidic platform that hosts brain-like 3D cultures ("optonets") whose activity and responses to flowing drugs are recorded optically. Optonets are viable, optically accessible 3D neural networks whose characteristics approximate cortical networks. The results demonstrate the
Seiji Kobuke et al.
Archives of oral biology, 60(9), 1263-1272 (2015-06-24)
Phosphophoryn (PP) is generated from the proteolytic cleavage of dentin sialophosphoprotein (DSPP). PP which contains tandem serine/asparatic acid rich repeats (SDrr) is known to enhance dentin mineralization. The nucleotide sequences coding SDrr are identified in the DSPP genes of toothed
Andrews, A.T.
Electrophoresis: Theory, Techniques, and Biochemical and Clinical Applications, 38-42 (1988)
Robyn M Murphy et al.
The Journal of physiology, 587(2), 443-460 (2008-11-26)
Whilst calsequestrin (CSQ) is widely recognized as the primary Ca2+ buffer in the sarcoplasmic reticulum (SR) in skeletal muscle fibres, its total buffering capacity and importance have come into question. This study quantified the absolute amount of CSQ isoform 1
Kimihiro Abe et al.
PLoS genetics, 10(10), e1004636-e1004636 (2014-10-10)
Temperate phages infect bacteria by injecting their DNA into bacterial cells, where it becomes incorporated into the host genome as a prophage. In the genome of Bacillus subtilis 168, an active prophage, SPβ, is inserted into a polysaccharide synthesis gene
I Niebroj-Dobosz et al.
Muscle & nerve, 12(4), 273-280 (1989-04-01)
The protein compositions of subcellular fractions of muscle obtained from 17 Duchenne dystrophy patients, 15 disease controls (10 different primary myopathies, 5 spinal muscular atrophy patients), and 10 normals were examined by polyacrylamide gel electrophoresis. The gels were stained with
Y Sharma et al.
The Journal of biological chemistry, 264(35), 20923-20927 (1989-12-15)
The interaction of the cationic carbocyanine dye Stains-all (1-ethyl-2-[3-(1-ethyl-naphthol[1,2-d]thiazolin-2-ylidene)-2- methylpropenyl]naphthol[1,2-d]thiazolium bromide) with the eye lens proteins crystallins has been studied. alpha- and gamma-crystallins do not bind the dye, while beta- and delta-crystallins do, consistent with the fact that the latter
Rehm H
Protein Biochemistry and Proteomics. The experimenter series (2006)
A G Krishna et al.
Indian journal of biochemistry & biophysics, 28(1), 30-33 (1991-02-01)
The carbocyanine dye Stains-all displays spectral colour shifts or metachromasia upon binding to proteins, polysaccharides and other anionic substrates. The conformational status of the binding region of the substrate appears to govern the metachromatic features of the bound Stains-all. We
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