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Hong-Sheng Dai et al.
Journal of virology, 87(20), 11231-11243 (2013-08-09)
Rational design and directed evolution are powerful tools to generate and improve protein function; however, their uses are mostly limited to enzyme and antibody engineering. Here we describe a directed-evolution strategy, named the tandem selection and enrichment system (TSES), and
Rita Polati et al.
Journal of proteomics, 76 Spec No., 10-27 (2012-07-28)
Macrophages play a critical role at the crossroad between iron metabolism and immunity, being able to store and recycle iron derived from the phagocytosis of senescent erythrocytes. The way by which macrophages manage non-heme iron at physiological concentration is still
Yoshiaki Tsuji
PloS one, 15(7), e0235563-e0235563 (2020-07-10)
Western blotting has been widely used for investigation of protein expression, posttranslational modifications, and interactions. Because western blotting usually involves heat-denaturation of samples prior to gel loading, clarification of detailed procedures for sample preparation have been omitted or neglected in
Wenhan Zhu et al.
Cell host & microbe, 27(3), 376-388 (2020-02-23)
During short-lived perturbations, such as inflammation, the gut microbiota exhibits resilience and reverts to its original configuration. Although microbial access to the micronutrient iron is decreased during colitis, pathogens can scavenge iron by using siderophores. How commensal bacteria acquire iron
Abhishek Asthana et al.
Scientific reports, 7(1), 9600-9600 (2017-08-31)
Prion disease-associated retinal degeneration is attributed to PrP-scrapie (PrPSc), a misfolded isoform of prion protein (PrPC) that accumulates in the neuroretina. However, a lack of temporal and spatial correlation between PrPSc and cytotoxicity suggests the contribution of host factors. We
Carla V Galmozzi et al.
Bio-protocol, 7(6), e2176-e2176 (2017-03-20)
Isolation of ribosomal particles is an essential step in the study of ribosomal components as well as in the analysis of trans-acting factors that interact with the ribosome to regulate protein synthesis and modulate the expression profile of the cell
Samuele Marro et al.
Haematologica, 95(8), 1261-1268 (2010-02-25)
Macrophages of the reticuloendothelial system play a key role in recycling iron from hemoglobin of senescent or damaged erythrocytes. Heme oxygenase 1 degrades the heme moiety and releases inorganic iron that is stored in ferritin or exported to the plasma
Hugh Dickinson et al.
Bio-protocol, 8(16), e2977-e2977 (2018-08-20)
Studies of pollen germination and post-germination development are not only essential for understanding plant reproduction but also are an excellent model system for tip-based growth. Here we describe easy, reproducible methods for germination and growth of pollen from the model
Kyle A Bauckman et al.
Autophagy, 12(5), 850-863 (2016-03-24)
Autophagy is a cellular recycling pathway, which in many cases, protects host cells from infections by degrading pathogens. However, uropathogenic Escherichia coli (UPEC), the predominant cause of urinary tract infections (UTIs), persist within the urinary tract epithelium (urothelium) by forming
Yu Jiang et al.
Fish physiology and biochemistry, 45(1), 365-374 (2018-10-27)
Iron overload increases the risk of osteoporosis, which leads to an increase in the incidences of bone fracture after menopause. In vitro studies have demonstrated that excess iron can inhibit osteoblast activity. Hepcidin, a central regulator of iron homeostasis, prevents
Hiroko Nobuta et al.
Cell stem cell, 25(4), 531-541 (2019-10-05)
Pelizaeus-Merzbacher disease (PMD) is an X-linked leukodystrophy caused by mutations in Proteolipid Protein 1 (PLP1), encoding a major myelin protein, resulting in profound developmental delay and early lethality. Previous work showed involvement of unfolded protein response (UPR) and endoplasmic reticulum
Tomoya Ohno et al.
Journal of biochemistry, 151(1), 109-114 (2011-10-14)
There is a little information about the effects of iron overload on cartilage metabolism. In the present study, we examined the effects of excess iron on the differentiation and mineralization of cultured chondrocytes, ATDC5 cells. We used ferric ammonium citrate
Yinghui Zhang et al.
Antioxidants & redox signaling, 13(7), 999-1009 (2010-04-22)
Cytosolic ferritins sequester and store iron, consequently protecting cells against iron-mediated free radical damage. However, the mechanisms of iron exit from the ferritin cage and reutilization are largely unknown. In a previous study, we found that mitochondrial ferritin (MtFt) expression
Sant-Rayn Pasricha et al.
Nature communications, 8(1), 403-403 (2017-09-03)
Hepcidin regulates systemic iron homeostasis. Suppression of hepcidin expression occurs physiologically in iron deficiency and increased erythropoiesis but is pathologic in thalassemia and hemochromatosis. Here we show that epigenetic events govern hepcidin expression. Erythropoiesis and iron deficiency suppress hepcidin via
Connie W Y Ha et al.
Cell, 183(3), 666-683 (2020-09-30)
A mysterious feature of Crohn's disease (CD) is the extra-intestinal manifestation of "creeping fat" (CrF), defined as expansion of mesenteric adipose tissue around the inflamed and fibrotic intestine. In the current study, we explore whether microbial translocation in CD serves
Hao-Han Guo et al.
Bone research, 9(1), 31-31 (2021-06-11)
Patients with Alzheimer's disease (AD) often have lower bone mass than healthy individuals. However, the mechanisms underlying this change remain elusive. Previously, we found that Tg2576 mice, an AD animal model that ubiquitously expresses Swedish mutant amyloid precursor protein (APPswe)
Michaela Hohnholt et al.
Neurochemical research, 35(8), 1259-1268 (2010-05-15)
The oligodendroglial cell line OLN-93 was used as model system to investigate the consequences of iron deprivation or iron excess on cell proliferation. Presence of ferric or ferrous iron chelators inhibited the proliferation of OLN-93 cells in a time and
Luca Valenti et al.
Arteriosclerosis, thrombosis, and vascular biology, 31(3), 683-690 (2010-12-25)
Increased body iron stores and hepcidin have been hypothesized to promote atherosclerosis by inducing macrophage iron accumulation and release of cytokines, but direct demonstration in human cells is lacking. The aim of this study was to evaluate the effect of
Hui Wang et al.
Molecular cell, 78(1), 31-41 (2020-03-04)
Cellular iron homeostasis is dominated by FBXL5-mediated degradation of iron regulatory protein 2 (IRP2), which is dependent on both iron and oxygen. However, how the physical interaction between FBXL5 and IRP2 is regulated remains elusive. Here, we show that the
Peng Jia et al.
Zhonghua yi xue za zhi, 92(31), 2214-2218 (2012-11-20)
To explore the effects of ferric ion on the differentiation from both RAW264.7 and bone marrow macrophages to osteoclast in vitro and bone resorption in vivo. In the presence of 50 ng/ml receptor activator of nuclear factor kappa-B ligand (RANKL)
C Salis et al.
Neurochemistry international, 61(5), 798-806 (2012-07-11)
The differentiation of myelin-forming Schwann cells (SC) is completed with the appearance of myelin proteins MBP and P(0) and a concomitant downregulation of markers GFAP and p75NTR, which are expressed by immature and adult non-myelin-forming SC. We have previously demonstrated
Yulia Pollak et al.
Journal of neural transmission (Vienna, Austria : 1996), 120(1), 37-48 (2012-03-27)
Iron accumulation and iron-related oxidative stress are involved in several pathological conditions and provide a rationale for the development of iron chelators as novel promising therapeutic strategies. Thus, we have recently synthesized multifunctional non-toxic, brain permeable iron chelating compounds, M30
Andrew E Armitage et al.
Journal of innate immunity, 8(5), 517-528 (2016-07-18)
Withdrawal of iron from serum (hypoferraemia) is a conserved innate immune antimicrobial strategy that can withhold this critical nutrient from invading pathogens, impairing their growth. Hepcidin (Hamp1) is the master regulator of iron and its expression is induced by inflammation.
Ryan M Mitchell et al.
Neurobiology of aging, 32(6), 1114-1123 (2009-06-30)
HFE gene variants are relatively common genetic variants in Caucasians. The H63D HFE genetic variant has been repeatedly associated with a number of neurodegenerative diseases. We developed neuroblastoma cell lines expressing different HFE polymorphisms to explore the mechanisms behind these
Sunggyu Yoon et al.
Molecules and cells, 40(12), 976-985 (2017-12-15)
Iron is an essential divalent ion for aerobic life. Life has evolved to maintain iron homeostasis for normal cellular and physiological functions and therefore imbalances in iron levels exert a wide range of consequences. Responses to iron dysregulation in blood
David J Lemler et al.
Breast cancer research : BCR, 19(1), 25-25 (2017-03-09)
Duodenal cytochrome b (DCYTB) is a ferrireductase that functions together with divalent metal transporter 1 (DMT1) to mediate dietary iron reduction and uptake in the duodenum. DCYTB is also a member of a 16-gene iron regulatory gene signature (IRGS) that
Jiadong Wu et al.
Bone, 121, 16-28 (2019-01-06)
Iron accumulation is an independent risk factor for type I osteoporosis, but the molecular mechanisms of the phenomenon are not well defined, and effective therapy has not been reported. Here, we found that the level of mTOR was increased both
T Takahara et al.
Nihon rinsho. Japanese journal of clinical medicine, 56(11), 2865-2869 (1998-12-16)
The high signal intensity from the intestinal fluid usually deteriorates the projection images of MR cholangiopancreatography (MRCP) because it superimposes on the biliary tract. FerriSeltz (Otsuka pharmaceutical, Tokushima, Japan; a ferric ammonium citrate-based positive oral contrast agent) solution in a
Iron excess limits HHIPL-2 gene expression and decreases osteoblastic activity in human MG-63 cells.
M Doyard et al.
Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA, 23(10), 2435-2445 (2012-01-13)
In order to understand mechanisms involved in osteoporosis observed during iron overload diseases, we analyzed the impact of iron on a human osteoblast-like cell line. Iron exposure decreases osteoblast phenotype. HHIPL-2 is an iron-modulated gene which could contribute to these
Peter E Burby et al.
Bio-protocol, 7(8) (2017-07-15)
A fundamental procedure for most modern biologists is the genetic manipulation of the organism under study. Although many different methods for editing bacterial genomes have been used in laboratories for decades, the adaptation of CRISPR/Cas9 technology to bacterial genetics has
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