Nucleic acid labeling and detection

Determine the labeling efficiency in terms of μg (expected yield of a standard labeling reaction is 20 μg of DIG labeled RNA per μg linearized template DNA after the DIG RNA labeling reaction).

DIG RNA Labeling Mix Protocol Troubleshooting

Determine the labeling efficiency in terms of μg (expected yield of a standard labeling reaction is 20 μg of DIG labeled RNA per μg linearized template DNA after the DIG RNA labeling reaction).

Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

Gelatin Blocking Buffer for Direct Detection Southern or Western Blotting

In order to specifically detect an antigen or target molecule immobilized on a solid support, unoccupied binding sites on the support must be blocked against binding by probe and detection molecules.

Dextran Sulfate

Dextran sulfates are supplied as the sodium salt forms, making them soluble and stable in water. Dextran sulfate contains approximately 17% sulfur which is equivalent to approximately 2.3 sulfate groups per glucosyl residue.

ABI3900 Synthesizer Columns with CUTAG CPG Frits

Next generation CPG Frit support for reliable, cost-effective oligo synthesis from Proligo®, for ABI 3900 and MerMade synthesizers

DIG Northern Starter Kit Protocol & Troubleshooting

In general, it is possible to use DNA probes for northern blots (PCR or random-primed DNA probes). It is however important to keep in mind that DNA probes are best detecting abundant messages, such as housekeeping genes.

NBT/BCIP Ready-to-Use Tablets Protocol Troubleshooting

NBT Protocol & Troubleshooting

There are several counterstains possible in combination with BM Purple (or NBT/BCIP in general), including FastGreen FCF and Nuclear Fast Red.

Oligonucleotide Melting Temperature

Learn about methods for calculating oligonucleotide melting temperature (Tm).