Enzyme activity assays

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EC编号

用于代谢研究的酶的完整EC编号列表。

磷酸二酯酶类

环核苷酸磷酸二酯酶 (PDEs) 催化 cAMP 和/或 cGMP 的水解。存在有11种不同的哺乳动物PDE家族。

G蛋白（异源三聚体）

由α，β和γ亚基所组成的异源三聚体G蛋白通过调节细胞内效应蛋白如酶和离子通道，可对由活化的七跨膜（7TM）受体产生的细胞外信号产生响应。

胆固醇酯化TEST

胆固醇酯化可改善运输。胆固醇酯可更轻松地封装在脂蛋白内部-增加了在血液中可轻松运输的胆固醇数量。

如何使用溶于硫酸铵悬液的酶

以硫酸铵悬浮液形式提供的酶的使用指南

组织蛋白酶B的酶活检测

组织蛋白酶B是具有广泛特异性的溶酶体半胱氨酸蛋白酶。本方案采用Nα–CBZ–Arg–Arg–7–酰胺基-4-甲基香豆素作为荧光检测组织蛋白酶B活性的底物。

Coenzyme A

Enzyme Reagent Coenzyme A (CoA, CoASH or HSCoA) is the key cofactor in first step of the TCA cycle, responsible for transferring the acetyl group from pyruvate oxidation to oxaloacetate yielding citrate. Available through Sigma-Aldrich online.

Enzymatic Method for Determining Branched Chain Amino Acids (BCAA Assay)

Assay protocol for the colorimetric detection of Branched Chain Amino Acids (BCAA) in biological samples using the alanine assay kit.

Enzyme Commission Numbers

Complete List of Enzyme Commission Numbers for metabolics research.

Tubulin

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells.

Cholesterol Esterification

Cholesterol undergoes esterification to improve transport. Cholesterol esters are more easily packaged into the interior of lipoproteins - increasing the quantity that can be readily transported in the blood stream.

Enzymes for Alternative Energy Research

We are committed to help unlock the today’s challenge of releasing the energy of glucose that nature has cleverly locked into lignocellulosic biomass. To the untrained eye, starch and cellulose structures appear almost identical. Yet the barriers between cellulosic biomass

Fibrinogen/Fibrin

Fibrinogen is a ~340 kDa glycoprotein synthesised in the liver. It is a hexamer composed of pairs of three peptide chains (α, β, and γ). Fibrinogen is an acute phase protein that is part of the coagulation cascade of

Drug Conjugate Analysis using β-Glucuronidases

Drug conjugate analysis and the enzymatic hydrolysis of glucuronides

Aprotinin

While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Compound Library Screening with LOPAC®1280 Small Molecules

Learn about compound library screening and explore our compound libraries, including our Library of Pharmacologically Active Compounds (LOPAC®1280) portfolio to experience the gold-standard collection of biologically annotated, ready-to-screen compounds.

Neuropeptidases

We offers many products related to neuropeptidases for your research needs.

InsR

We offers many products related to InsR for your research needs.

Phospholipase D

Phosphatidylcholine (PC)-specific phospholipase D (PLD) enzymes catalyze the hydrolysis of PC to generate choline and phosphatidic acid. These enzymes belong to a superfamily of phosphatidyltransferases with a characteristic unique duplicated catalytic domain motif, the PLD or " HKD"

Phosphodiesterases

Cyclic nucleotide phosphodiesterases (PDEs) catalyze the hydrolysis of cAMP and/or cGMP. There are 11 different mammalian PDE families.

Neurotensin Receptors

Neurotensin is a 13 amino acid regulatory peptide found mainly in gut and brain. Intestinal neurotensin is produced by a discrete population of endocrine cells (N cells) scattered throughout the jejuno-ileal mucosa.

Nuclear Receptors (PPARs)

Peroxisome proliferator activated receptors (PPARs) are members of the nuclear hormone receptor superfamily of ligand-activated transcription factors that are related to retinoid, steroid and thyroid hormone receptors.

Horseradish Peroxidase (HRP) Enzymes

Discover our peroxidase from horseradish enzymes, products, substrates, and inhibitors for your ELISA, immunoassay, and protein application needs.

Decorin

Decorin is a small proteoglycan, which has been found associated with collagen fibrils in all connective tissues. In the skeletal system it is found primarily in the extracellular matrix of articular cartilage, which covers the surface of adult bones in

HMG-CoA Reductase (HMGR) Assay Kit

3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) is a transmembrane glycoprotein, located on the endoplasmic reticulum.

Enzymatic Assay of Cathepsin B

Cathepsin B is a lysosomal cysteine proteinase with broad specificity. This protocol uses Nα–CBZ–Arg–Arg–7–amido–4–methylcoumarin as the substrate for fluorometric detection of Cathepsin B activity.

Mucins, Glycosylated Proteins, Properties & Diagram

Mucins are a diverse family of densely glycosylated proteins. Mucin domains within the protein core are rich in threonine, serine and hydroxyproline enabling post-translational O-glycosylation.

Bioluminescent Firefly Luciferase Assays

Firefly luciferase is a widely used bioluminescent reporter for studying gene regulation and function. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues.

Dipeptidyl Peptidase IV

Native DPPIV is a ubiquitous type II transmembrane glycoprotein and a serine protease of the S9 prolyl-oligopeptidase family. In vivo, it is synthesized with a signal peptide which functions as the membrane anchoring domain.

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