Fatty Acid Extraction Kit, High Standard (MAK338) - Technical Bulletin
Fatty Acid Extraction Kit, High Standard
Catalog Number MAK338
Store at Room Temperature
TECHNICAL BULLETIN
Fatty Acid Methylation Kit (MAK224) - Technical Bulletin
Fatty Acid Methylation Kit
Manufactured by Nacalai Tesque, Inc.
Catalog Number MAK224
Store at Room Temperature
TECHNICAL
including phospholipids, triglycerides, and
fatty acids. The Fatty Acid Extraction kit enables the
extraction of these lipids in one step. The extracted
lipids can then be transesterified and quantified
phospholipids, free fatty acids and cholesteryl
esters may be present. The Fatty Acid Extraction Kit for
Pet Food enables the extraction of these lipids in one
step. The extracted lipids can then be
sequentially.
4. Mix and extract fatty acid methyl esters (FAME) using 2 mL n-hexane twice.
5. FAME are performed as duplicates by injecting approximately 80 μL of extract into a gas chromatograph
Short Chain Fatty Acids Kit
Catalog Number SBR00030
Storage Temperature -20 C
TECHNICAL BULLETIN
Product Description
Short chain fatty acids (SCFAs) are saturated fatty
acids with less
Centrifuge the sample for 5 minutes at 3,500 rpm.
A fatty layer may form and solidify at the top of the
extract. Use the sample underneath the fatty layer
for the assay.
Procedure
cheese
extract
5. TMB Substrate (916T001, Blue Cap): 12 mL of
stabilized urea peroxide and
3,3,5,5-tetramethylbenzidine (TMB).
6. Stop Solution (946P001, Red Cap): 12 mL of Acidic
Solution
precursor to hormones. The
Cholesterol Extraction Kit enables the extraction of
sterols such as cholesterol from biological samples in
two simple steps. The extracted cholesterol can then be
saponified
Octanoic acid, commonly known as caprylic acid, is a
straight-chain fatty acid found in various vegetable and
animal sources, such as coconut oil, palm nut oil, and
butter fat.
Caprylic acid has been
designates a class of
enzymes that hydrolyzes the sn-2 ester of
glycerophospholipids to produce a fatty acid and a
lysophospholipid. Nine different groups of mammalian
PLA2s have been identified. Group
Fatty Acid Methyl Esters Standard Mixture
Catalog Number SMB00937
Product Description
The Fatty Acid Methyl Esters Standard
Mixture is a metabolite mixture comprised of
13 fatty acids
Technical Bulletin
Free Fatty Acid Assay Kit
Catalogue Number MAK466
Product Description
Fatty acids are aliphatic monocarboxylic acids
ubiquitously found in animal or vegetable
phosphatidylcholine have
fatty acid contents of approximately 13% C16:0
(palmitic), 4% C18:0 (stearic), 10% C18:1(oleic),
64% C18:2 (linoleic), and 6% 18:3 (linolenic) with
other fatty acids being minor contributors
important metabolic processes of the
peroxisome is the β-oxidation of long and very long
chain fatty acids. Peroxisomes are also involved in
detoxification of the cell by the enzyme catalase that
decomposes
units,
1 sorbitol, and 1 lauric acid as the primary fatty acid.
Fatty acid constituents of this product are determined
by transesterification to yield fatty acid methyl esters,
which are identified
units,
1 sorbitol, and 1 lauric acid as the primary fatty acid.
Fatty acid constituents of this product are determined
by transesterification to yield fatty acid methyl esters,
which are identified
oxide units, 1 sorbitan and lauric acid as the primary fatty acid. Fatty acid
constituents of this product are determined by transesterification to yield fatty acid methyl esters which are
identified
units,
1 sorbitol, and 1 lauric acid as the primary fatty acid.
Fatty acid constituents of this product are determined
by transesterification to yield fatty acid methyl esters,
which are identified
mg/mL extract
3b: 75 mg/mL extract
Clean-up of extracts of
foods of plant origin with
high matrix-load prior to
the analysis of basic and
neutral pesticides
Extracts from
extraction modules
well and incubate for 10 minutes at
room temperature to convert triglycerides to
glycerol and fatty acids.
2. Set up the Master Reaction Mix according to
the scheme in Table 1 (colorimetric)
yolk phosphatidylcholine
have fatty acid contents of approximately 33% C16:0
(palmitic), 13% C18:0 (stearic), 31% C18:1(oleic), and
15% C18:2 (linoleic) (other fatty acids being minor
contributors), which