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Jinfang Zhao et al.
Wei sheng wu xue bao = Acta microbiologica Sinica, 53(4), 328-337 (2013-07-19)
In this study, we constructed a recombinant Escherichia coli strain for the production of high-purity L-lactic acid, using a homoethanol fermenting mutant E. coli SZ470 (deltafrdBC deltaldhA deltaackA deltafocA-pflB deltapdhR: :pflBp6-pflBrbs-aceEF-lpd) as the starting strain. By using homologous recombination, we
Ekta Lachmandas et al.
PloS one, 10(2), e0117941-e0117941 (2015-02-11)
Type 2 diabetes mellitus is an established risk factor for tuberculosis but the underlying mechanisms are largely unknown. We examined the effects of hyperglycaemia, a hallmark of diabetes, on the cytokine response to and macrophage infection with Mycobacterium tuberculosis. Increasing
Victor Castro-Fernandez et al.
The FEBS journal, 281(8), 2017-2029 (2014-05-27)
In some archaea, the phosphorylation of glucose and fructose 6-phosphate (fructose 6P) is carried out by enzymes that are specific for either substrate and that use ADP as phosphoryl donor. In the hyperthermophilic archaeon Methanocaldococcus jannaschii, a bifunctional enzyme able
D A Gálico et al.
Journal of inorganic biochemistry, 140, 160-166 (2014-08-12)
Solid-state compounds of the general formulae [ML3] (M=Gd, Tb, Dy, Ho, Er, Tm, Yb, Lu, Y; L=ketoprofen) were synthesized and characterized using infrared, diffuse reflectance and luminescence spectroscopies. IR data suggested that the carboxylate group in ketoprofen is coordinated to
Yiwei Li et al.
Lab on a chip, 15(15), 3203-3210 (2015-07-02)
Active control over the cell gradient is essential for understanding biological systems and the reconstitution of the functionality of many types of tissues, particularly for organ-on-a-chip. Here, we propose a three-dimensional (3D) microfluidic strategy for generating controllable cell gradients. In
Andrew N Carley et al.
Circulation. Heart failure, 8(1), 119-127 (2014-11-27)
The failing heart displays increased glycolytic flux that is not matched by a commensurate increase in glucose oxidation. This mismatch induces increased anaplerotic flux and inefficient glucose metabolism. We previously found adult transgenic mouse hearts expressing the fetal troponin I
Jannis E Meents et al.
The journal of headache and pain, 16, 57-57 (2015-06-26)
The capsaicin and heat responsive ion channel TRPV1 is expressed on trigeminal nociceptive neurons and has been implicated in the pathophysiology of migraine attacks. Here we investigate the efficacy of two TRPV1 channel antagonists in blocking trigeminal activation using two
Rebekah E Wharton et al.
Journal of immunology (Baltimore, Md. : 1950), 194(9), 4387-4396 (2015-03-31)
Invasive aspergillosis (IA) resulting from infection by Aspergillus fumigatus is a leading cause of death in immunosuppressed populations. There are limited therapeutic options for this disease and currently no vaccine. There is evidence that some anti-A. fumigatus mAbs can provide
Jasreen K Sekhon et al.
Bioresource technology, 192, 451-460 (2015-06-17)
Enzyme-assisted aqueous extraction processing (EAEP) is an environmentally-friendly alternative to solvent and mechanical oil extraction methods, and can achieve ∼ 97% oil recovery from soybeans. The present study utilized soy skim (protein rich) and insoluble fiber (IF; carbohydrate rich), both
Mustapha Aouida et al.
Journal of bioscience and bioengineering, 120(4), 364-371 (2015-04-25)
Targeted engineering of microbial genomes holds much promise for diverse biotechnological applications. Transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats/Cas9 systems are capable of efficiently editing microbial genomes, including that of Saccharomyces cerevisiae. Here, we demonstrate
Girish Ramachandran et al.
PLoS neglected tropical diseases, 9(1), e3394-e3394 (2015-01-09)
Invasive non-typhoidal Salmonella (iNTS) are an important cause of septicemia in children under the age of five years in sub-Saharan Africa. A novel genotype of Salmonella enterica subsp. enterica serovar Typhimurium (multi-locus sequence type [ST] 313) circulating in this geographic
Accidental hypoglycaemia caused by an arterial flush drug error.
M Thirugnanam et al.
Anaesthesia, 69(5), 524-525 (2014-04-18)
David Wilkinson et al.
Diving and hyperbaric medicine, 45(1), 30-36 (2015-05-13)
The onset of insulin resistance is an important metabolic event in the development of type 2 diabetes. For patients with type 2 diabetes, we recently showed that peripheral insulin sensitivity was increased during hyperbaric oxygen treatment (HBOT). This study aims
Yong-Min Choi et al.
PloS one, 10(8), e0135083-e0135083 (2015-08-08)
The chemotherapeutic use of cisplatin is limited by its severe side effects. In this study, by conducting different omics data analyses, we demonstrated that cisplatin induces cell death in a proximal tubular cell line by suppressing glycolysis- and tricarboxylic acid
Sachio Yamamoto et al.
Analytical and bioanalytical chemistry, 407(20), 6201-6206 (2015-06-06)
A novel method was developed for D/L-isomeric separation of aldopentoses and aldohexoses as their (S)-(+)-4-(N,N-dimethylaminosulfonyl)-7-(3-aminopyrrolidin-1-yl)-2,1,3-benzoxadiazole derivatives using phenylboronate buffer containing sodium dodecyl sulfate as a background electrolyte. The combination of derivatization with a chiral labeling reagent and micellar electrokinetic chromatography
Maria Belen Lopez et al.
Journal of cellular physiology, 230(12), 2936-2950 (2015-04-23)
We have previously demonstrated a crucial role of nuclear protein 1 (NUPR1) in tumor development and progression. In this work, we report the functional characterization of a novel Nupr1-like isoform (NUPR1L) and its functional interaction with the protumoral factor NUPR1.
Misato Iwashita et al.
Development (Cambridge, England), 141(19), 3793-3798 (2014-09-25)
Accumulating evidence implicates the significance of the physical properties of the niche in influencing the behavior, growth and differentiation of stem cells. Among the physical properties, extracellular stiffness has been shown to have direct effects on fate determination in several
Sahra Borges et al.
Molecular cancer therapeutics, 14(6), 1306-1316 (2015-04-09)
Invasive ductal carcinomas (IDC) of the breast are associated with altered expression of hormone receptors (HR), amplification or overexpression of HER2, or a triple-negative phenotype. The most aggressive cases of IDC are characterized by a high proliferation rate, a great
Pamela Romo-Rodríguez et al.
Chemosphere, 134, 563-570 (2015-01-13)
The Cr(VI) reducing capability of growing cells of the environmental A. tubingensis Ed8 strain is remarkably efficient compared to reference strains A. niger FGSC322 and A. tubingensis NRRL593. Extracellular glucose oxidase (GOX) activity levels were clearly higher in colonies developed
Pedro García-Coronado et al.
Polymers, 12(9) (2020-09-17)
The state diagram, which is defined as a stability map of different states and phases of a food as a function of the solid content and temperature, is regarded as fundamental approach in the design and optimization of processes or
L Ia Timen et al.
Eksperimental'naia i klinicheskaia gastroenterologiia = Experimental & clinical gastroenterology, (5)(5), 89-92 (2014-02-08)
Glucose and ascorbate used for endoscopic hemostasis metabolic preventive to prevent recurrent ulcer bleeding. Unacceptable joint use in endohemostasis ascorbate and hydrogen peroxide.
W Grünberg et al.
Journal of dairy science, 98(8), 5385-5400 (2015-06-01)
Hypophosphatemia is a common finding in periparturient and anorectic cattle. Although the clinical relevance of hypophosphatemia in cattle is uncertain, it has been empirically associated with persistent recumbency, specifically in periparturient dairy cows. The objective of the present study was
I N Kurchenko et al.
Mikrobiolohichnyi zhurnal (Kiev, Ukraine : 1993), 75(5), 47-51 (2014-02-01)
A comparative study of growth characteristics and peculiarities of glucose utilization by Fusarium poae and Penicillium funiculosum strains isolated from different habitats was conducted. It was found that F. poae and P. funiculosum strains differ as to their growth characteristics.
Abdelali Agouni et al.
Biochemical pharmacology, 92(4), 607-617 (2014-12-03)
Growing evidence suggests that hepatic-insulin resistance is sufficient to promote progression to cardiovascular disease. We have shown previously that liver-specific protein-tyrosine-phosphatase 1B (PTP1B) deficiency improves hepatic-insulin sensitivity and whole-body glucose homeostasis. The aim of this study was to investigate the
Daiki Goto et al.
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 31(11), 1143-1149 (2015-11-13)
It is difficult to control the electrophoretic mobility in order to obtain high resolution among saccharides in complex samples. We report herein on a new affinity capillary electrophoresis (ACE) method for an anionic monosaccharide, N-acetylneuraminic acid (Neu5Ac), which is important
Sihyung Yang et al.
Antimicrobial agents and chemotherapy, 58(7), 4064-4074 (2014-05-07)
Human African trypanosomiasis (HAT), a neglected tropical disease, is fatal without treatment. Pentamidine, a cationic diamidine, has been used to treat first-stage (hemolymphatic) HAT since the 1940s, but it is ineffective against second-stage (meningoencephalitic, or central nervous system [CNS]) infection.
Yoichi Maekawa et al.
Nature medicine, 21(1), 55-61 (2014-12-17)
CD4+ T cells differentiate into memory T cells that protect the host from subsequent infection. In contrast, autoreactive memory CD4+ T cells harm the body by persisting in the tissues. The underlying pathways controlling the maintenance of memory CD4+ T
Elisabeth Christiansen et al.
The British journal of nutrition, 113(11), 1677-1688 (2015-04-29)
Various foods are associated with effects against metabolic diseases such as insulin resistance and type 2 diabetes; however, their mechanisms of action are mostly unclear. Fatty acids may contribute by acting as precursors of signalling molecules or by direct activity
A Wagner et al.
European journal of clinical nutrition, 69(4), 436-441 (2014-10-23)
The omega-3 index (the summed percentage content of eicosapentaenoic and docosahexaenoic acids in red blood cells) is associated with a lower risk of fatal coronary heart disease and sudden cardiac death. We aimed to determine which socio-demographic, behavioural or clinical
Haijiang Wu et al.
Molecular and cellular endocrinology, 417, 27-35 (2015-09-13)
Renal tubular epithelial cells (RTEC) apoptosis, which plays a key role in the pathogenesis and progression of diabetic nephropathy (DN), is believed to be contributive to the hyperglycemia-induced kidney failure, though the exact mechanisms remain elusive. In this study, we
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