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关键词:'H1256'
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P Portolés et al.
Journal of immunological methods, 129(1), 105-109 (1990-05-08)
A method for the vectorial radioiodination of CD4 and other membrane proteins having few or no tyrosine residues in the extracytoplasmic domains is described. Incubation of the cells with sulfosuccinimidyl (hydroxyphenyl) propionate (sulfo-SHPP), a water-soluble derivative of the Bolton-Hunter reagent
E Marshall et al.
Molecular and biochemical parasitology, 15(3), 295-304 (1985-06-01)
The specificity of a range of 125I labelling techniques (Chloramine T, Iodogen, Bolton and Hunter reagent, lactoperoxidase and iodosulfanilic acid) to the surface of the filarial nematode Brugia pahangi was evaluated by autoradiography of sections of labelled worms and of
Sander M J van Duijnhoven et al.
Journal of nuclear medicine : official publication, Society of Nuclear Medicine, 56(9), 1422-1428 (2015-07-15)
Radioimmunotherapy and nuclear imaging (immuno-PET/SPECT) of cancer with radiometal-labeled antibody fragments or peptides is hampered by low tumor-to-kidney ratios because of high renal radiometal retention. Therefore, we developed and evaluated a pretargeting strategy using click chemistry in vivo to reduce
A J Tenner et al.
Journal of immunology (Baltimore, Md. : 1950), 127(2), 648-653 (1981-08-01)
A new procedure for isolating human C1q from serum or plasma is described. The method, that is highly selective, rapid and involves minimal handling, yields fully active, immunoglobulin-free unaggregated C1q. Several different methods of radiolabeling C1q are compared. These include
M Rack et al.
Pflugers Archiv : European journal of physiology, 400(4), 403-408 (1984-04-01)
Several reagents that react with sulfhydryl and amino groups were applied to voltage-clamped single nerve fibres of the frog. The fibres were exposed to comparable amounts of the chemical reagents for relatively short times. 3-(p-Hydroxyphenyl)propionic acid N-hydroxysuccinimide ester (HPPS), a
Radioimmunoassay of iodinated gentamicin
Broughton, Alan and Strong, James E
Clinica Chimica Acta; International Journal of Clinical Chemistry, 66(1), 125-129 (1976)
Trans-cyclooctene tag with improved properties for tumor pretargeting with the diels-alder reaction.
Raffaella Rossin et al.
Molecular pharmaceutics, 11(9), 3090-3096 (2014-08-01)
Radioimmunotherapy (RIT) of solid tumors is hampered by low tumor-to-nontumor (T/NT) ratios of the radiolabeled monoclonal antibodies resulting in low tumor doses in patients. Pretargeting technologies can improve the effectiveness of RIT in cancer therapy by increasing this ratio. We
Comparative methods for the radiolabeling of peptides.
A E Bolton
Methods in enzymology, 124, 18-29 (1986-01-01)
Jin-Ho Kim et al.
Molecular pharmaceutics, 12(4), 1230-1241 (2015-02-17)
Water-insoluble anticancer drugs, including paclitaxel, present severe clinical side effects when administered to patients, primarily associated with the toxicity of reagents used to solubilize the drugs. In efforts to develop alternative formulations of water-insoluble anticancer drugs suitable for intravenous administration
M P Merker et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 4(12), 3040-3048 (1990-09-01)
To study the biochemical characteristics of endothelium in vivo, we radioiodinated endothelial membrane proteins of the perfused rabbit lung using a water soluble analog of the Bolton-Hunter reagent, 125I-sulfosuccinimidyl (hydroxyphenyl) propionate (125I-s-SHPP). This technique led to a 10-fold increase in
R H Raja et al.
Analytical biochemistry, 139(1), 168-177 (1984-05-15)
Present procedures to obtain radiolabeled hyaluronic acid derivatives are limited to low-specific-activity isotopes and small amounts of material, and often involve multiple points of chemical modification within the polymer. A synthesis has been developed which affords large quantities of a
Preparation of 125-I-labeled oligosaccharide derivatives with the aid of 3-(4-hydroxyphenyl) propionic acid N-hydroxysuccinimide ester
Himmelspach K, et al.
Febs Letters, 75(1-2), 154-158 (1977)
A E Bolton et al.
The Biochemical journal, 133(3), 529-539 (1973-07-01)
1. A new method is described for labelling proteins to high specific radioactivities with (125)I. The protein is treated with a (125)I-labelled acylating agent, iodinated 3-(4-hydroxyphenyl)propionic acid N-hydroxysuccinimide ester, which reacts with free amino groups in the protein molecule to
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