Various Immunoglobulin Light Chains.
Species Protein L Binding
Human kappa I ++++
Human kappa II !
Human kappa III ++++
Human kappa IV ++++
Human lambda I-IV !
Human lambda IV +
Mouse kappa I ++++
Mouse
Various Immunoglobulin Light Chains.
Species Protein L Binding
Human kappa I ++++
Human kappa II !
Human kappa III ++++
Human kappa IV ++++
Human lambda I-IV !
Human lambda IV +
Mouse kappa I ++++
Mouse
chains, kappa and
lambda. Each immunoglobulin molecule contains either
kappa or lambda light chains. In mouse, there is only
one kappa light chain class, but there are three lambda
chain classes ( 1- 3).
mammalian) immunoglobulins
contain one of two light chain types, either kappa or
lambda. Variability in amino acid sequence occurs on
the N-terminus of the light chains and, together with the
havy chain
the
mouse immunoglobulin molecule. Monoclonal Anti-1
Mouse Lambda Light Chains recognizes an epitope
located in the V�2 domain of the mouse immuno-315
globulin molecule; this V (variable) domain
Disease Control (CDC)1.
Monoclonal Anti Human Lambda Light Chains is specific
for the lambda light chains of human immunoglobulin (all
isotypes). It does not react with human kappa light
chains, using
Mouse monoclonal specifically
recognizes lambda light chains of human
immunoglobulins (all isotypes), and is non-reactive with
kappa light chains. The antibody recognizes both the
heavy chain-bound and
bridges. The
various types of human (and other mammalian)
immunoglobulins contain one of the two existing light
chain types, either kappa or lambda in which multiple
structural differences are reflected
of the mouse immunoglobulin molecule. This V
(variable) domain epitope is also expressed on λ1 and λ3
chains.1 The product detects mouse lambda light chain
cell surface immunoglobulins. It does not
playing a role in their destruction.
Immunoglobulins (Ig) can be classified by isotype, classes that differ in function and antigen
response due to structure variability. Five major isotypes have been identified
+ Moderate/Slight Affinity - No Affinity
Table 1. Relative affinity of Protein A and Protein G for various Immunoglobulins.
Antibody purification using the Amicon® Pro Affinity Concentration
(± in Table 1), we recommend preliminary studies to
determine the optimal matrix for maximal recovery.
Table 1. Relative affinity of Protein A and Protein G for various immunoglobulins.
Antibody
of kappa light chain isotype and therefore will react only
with anti-Human Kappa detection reagent.
2. Preparation of Working Standards
Label 6 polypropylene microfuge tubes Standard 1 through
R7757)
9. ViaCount Reagent (Part No. 4700-0040)
10. Isotype controls: rat IgG2a, kappa FITC and APC; rat IgG2b, kappa PE
Precautions
• The instructions provided have been designed to optimize the