l4777

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Screening of solvents for favoring hydrolytic activity of Candida antarctica Lipase B.

Bartłomiej Zieniuk et al.

Bioprocess and biosystems engineering, 43(4), 605-613 (2019-11-18)

Lipases are a group of enzymes of considerable significance in organic synthesis, among which Candida antarctica lipase B (CALB) is one of the most widely studied enzymes. The activity of the biocatalyst has been intensively characterized in many organic media

Isolation and cDNA sequence of human postheparin plasma hepatic triglyceride lipase.

G A Martin et al.

The Journal of biological chemistry, 263(22), 10907-10914 (1988-08-05)

Hepatic triglyceride lipase (H-TGL) was isolated from human postheparin plasma by column chromatography on heparin-Sepharose and phenyl-Sepharose and immunoaffinity chromatography with monoclonal antibodies. The purified enzyme had an apparent molecular weight of 65,000 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and

A common variant in PNPLA3, which encodes adiponutrin, is associated with liver fat content in humans.

A Kotronen et al.

Diabetologia, 52(6), 1056-1060 (2009-02-19)

It has recently been suggested that the rs738409 G allele in PNPLA3, which encodes adiponutrin, is strongly associated with increased liver fat content in three different ethnic groups. The aims of the present study were as follows: (1) to try

Isolation and characterization of the human hepatic lipase gene.

D Ameis et al.

The Journal of biological chemistry, 265(12), 6552-6555 (1990-04-25)

Overlapping bacterial phage and cosmid genomic clones were isolated spanning an area of approximately 60 kilobases that contains the human hepatic lipase (HL) gene. It is composed of 9 exons spanning approximately 35 kilobases of DNA. The entire coding regions

Facilitating chemical and biochemical experiments with electronic microcontrollers and single-board computers.

Gurpur Rakesh D Prabhu et al.

Nature protocols, 15(3), 925-990 (2020-01-31)

Since the advent of modern science, researchers have had to rely on their technical skills or the support of specialized workshops to construct analytical instruments. The notion of the 'fourth industrial revolution' promotes construction of customized systems by individuals using

A quantitative atlas of mitotic phosphorylation.

Noah Dephoure et al.

Proceedings of the National Academy of Sciences of the United States of America, 105(31), 10762-10767 (2008-08-02)

The eukaryotic cell division cycle is characterized by a sequence of orderly and highly regulated events resulting in the duplication and separation of all cellular material into two newly formed daughter cells. Protein phosphorylation by cyclin-dependent kinases (CDKs) drives this

Endothelial lipase modulates pressure overload-induced heart failure through alternative pathway for fatty acid uptake.

Hideto Nakajima et al.

Hypertension (Dallas, Tex. : 1979), 61(5), 1002-1007 (2013-03-06)

Lipoprotein lipase has been considered as the only enzyme capable of generating lipid-derived fatty acids for cardiac energy. Endothelial lipase is another member of the triglyceride lipase family and hydrolyzes high-density lipoproteins. Although endothelial lipase is expressed in the heart

Cloning of a unique lipase from endothelial cells extends the lipase gene family.

K Hirata et al.

The Journal of biological chemistry, 274(20), 14170-14175 (1999-05-13)

A new lipoprotein lipase-like gene has been cloned from endothelial cells through a subtraction methodology aimed at characterizing genes that are expressed with in vitro differentiation of this cell type. The conceptual endothelial cell-derived lipase protein contains 500 amino acids

Preparation of magnetic Fe3O4@SiO2 nanoparticles for immobilization of lipase.

Wei Liu et al.

Journal of nanoscience and nanotechnology, 14(4), 3068-3072 (2014-04-17)

Magnetic Fe3O4 nanoparticles were prepared with coprecipitatation method and covered with SiO2 to form the core-shell Fe3O4@SiO2 nanoparticles. Then the nanoparticles were modified with glutaradehyde for functionalization of the surface to aldehyde groups. The transmission electron microscopy confirmed the core-shell

Covalent Incorporation of Trehalose within Hydrogels for Enhanced Long-Term Functional Stability and Controlled Release of Biomacromolecules.

Timothy M O'Shea et al.

Advanced healthcare materials, 4(12), 1802-1812 (2015-06-20)

Hydrogels with covalently incorporated trehalose are synthesized using thiol-ene Michael addition. Trehalose hydrogels afford prolonged stabilization and -controlled release of model enzymes in vitro and in vivo as well as preservation of protein stability under heat and -lyophilization stressors. Strong

Expression, regulation, and triglyceride hydrolase activity of Adiponutrin family members.

Andrew C Lake et al.

Journal of lipid research, 46(11), 2477-2487 (2005-09-10)

Adiponutrin and a related protein, adipocyte triglyceride lipase (ATGL; also known as Desnutrin), were recently described as adipocyte-specific proteins with lipid hydrolase activity. Using bioinformatics, we identified three additional Adiponutrin family members (GS2, GS2-Like, and PNPLA1). Here, we report on

High-fat diet-mediated lipotoxicity and insulin resistance is related to impaired lipase expression in mouse skeletal muscle.

Pierre-Marie Badin et al.

Endocrinology, 154(4), 1444-1453 (2013-03-09)

Elevated expression/activity of adipose triglyceride lipase (ATGL) and/or reduced activity of hormone-sensitive lipase (HSL) in skeletal muscle are causally linked to insulin resistance in vitro. We investigated here the effect of high-fat feeding on skeletal muscle lipolytic proteins, lipotoxicity, and

Tissue expression pattern and polymorphism of G0S2 gene in porcine.

Yanzhi Jiang et al.

Gene, 539(1), 173-179 (2014-02-04)

Adipose triglyceride lipase (ATGL), catalyzing the initial step of hydrolysis of triacylglycerol (TAG) in adipocytes, has been known to be inhibited by G0/G1 switch protein 2 (G0S2). In this study, we determined tissue expression pattern and polymorphism of G0S2 gene

Identification, cloning, expression, and purification of three novel human calcium-independent phospholipase A2 family members possessing triacylglycerol lipase and acylglycerol transacylase activities.

Christopher M Jenkins et al.

The Journal of biological chemistry, 279(47), 48968-48975 (2004-09-15)

Genetic knockout of hormone-sensitive lipase in mice has implicated the presence of other intracellular triacylglycerol (TAG) lipases mediating TAG hydrolysis in adipocytes. Despite intense interest in these TAG lipases, their molecular identities thus far are largely unknown. Sequence data base

The DNA sequence of the human X chromosome.

Mark T Ross et al.

Nature, 434(7031), 325-337 (2005-03-18)

The human X chromosome has a unique biology that was shaped by its evolution as the sex chromosome shared by males and females. We have determined 99.3% of the euchromatic sequence of the X chromosome. Our analysis illustrates the autosomal

Fat mobilization in adipose tissue is promoted by adipose triglyceride lipase.

Robert Zimmermann et al.

Science (New York, N.Y.), 306(5700), 1383-1386 (2004-11-20)

Mobilization of fatty acids from triglyceride stores in adipose tissue requires lipolytic enzymes. Dysfunctional lipolysis affects energy homeostasis and may contribute to the pathogenesis of obesity and insulin resistance. Until now, hormone-sensitive lipase (HSL) was the only enzyme known to

Human hepatic lipase mutations and polymorphisms.

R A Hegele et al.

Human mutation, 1(4), 320-324 (1992-01-01)

Human hepatic lipase (HL) is a 477 residue glycoprotein that hydrolyzes triglycerides from plasma lipoproteins. Familial HL deficiency is a rare recessive disorder that is characterized by premature atherosclerosis and abnormal circulating lipoproteins. While studying the HL gene from the

Molecular cloning of a human gastric lipase and expression of the enzyme in yeast.

M W Bodmer et al.

Biochimica et biophysica acta, 909(3), 237-244 (1987-08-25)

The molecular cloning of a cDNA coding for human gastric lipase and its expression in yeast is described. A lipase present in human gastric aspirates was purified and its N-terminal amino-acid sequence was determined. This was found to be homologous

The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

Daniela S Gerhard et al.

Genome research, 14(10B), 2121-2127 (2004-10-19)

The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random

Adiponutrin: A new gene regulated by energy balance in human adipose tissue.

Yong-Ming Liu et al.

The Journal of clinical endocrinology and metabolism, 89(6), 2684-2689 (2004-06-08)

Adiponutrin is a newly identified nonsecreted adipocyte protein regulated by changes in energy balance in rodents. We documented the influence of energy balance modification on adiponutrin gene expression in humans. We investigated the mRNA expression in sc adipose tissue of

Structure of the human hepatic triglyceride lipase gene.

S J Cai et al.

Biochemistry, 28(23), 8966-8971 (1989-11-14)

The structure of the human hepatic triglyceride lipase gene was determined from multiple cosmid clones. All the exons, exon-intron junctions, and 845 bp of the 5' and 254 bp of the 3' flanking DNA were sequenced. Comparison of the exon

The DNA sequence of human chromosome 22.

I Dunham et al.

Nature, 402(6761), 489-495 (1999-12-11)

Knowledge of the complete genomic DNA sequence of an organism allows a systematic approach to defining its genetic components. The genomic sequence provides access to the complete structures of all genes, including those without known function, their control elements, and

Lipase member H is a novel secreted protein selectively upregulated in human lung adenocarcinomas and bronchioloalveolar carcinomas.

Yasuhiro Seki et al.

Biochemical and biophysical research communications, 443(4), 1141-1147 (2014-01-02)

Lung cancer is one of the most frequent causes of cancer-related death worldwide. However, molecular markers for lung cancer have not been well established. To identify novel genes related to lung cancer development, we surveyed publicly available DNA microarray data

Saturated fat-rich diet increases fetal lipids and modulates LPL and leptin receptor expression in rat placentas.

M B Mazzucco et al.

The Journal of endocrinology, 217(3), 303-315 (2013-03-14)

Metabolic alterations in obese and overweight mothers impact the placenta and the fetus, leading to anomalies in fetal growth and lipid accretion. The primary aim of the study was to examine the effect of a saturated fat-rich diet (FD) on

Human liver arylacetamide deacetylase. Molecular cloning of a novel esterase involved in the metabolic activation of arylamine carcinogens with high sequence similarity to hormone-sensitive lipase.

M R Probst et al.

The Journal of biological chemistry, 269(34), 21650-21656 (1994-08-26)

Microsomal arylacetamide deacetylase (DAC) competes against the activity of cytosolic arylamine N-acetyltransferase, which catalyzes one of the initial biotransformation pathways for arylamine and heterocyclic amine carcinogens in many species and tissues. Activity determination and immunoblot analysis of DAC in human

Contribution of novel ATGL missense mutations to the clinical phenotype of NLSD-M: a strikingly low amount of lipase activity may preserve cardiac function.

Daniela Tavian et al.

Human molecular genetics, 21(24), 5318-5328 (2012-09-20)

The lack of adipose triglyceride lipase (ATGL), a patatin-like phospholipase domain-containing enzyme that hydrolyzes fatty acids from triacylglycerol (TAG) stored in multiple tissues, causes the autosomal recessive disorder neutral lipid storage disease with myopathy (NLSD-M). In two families of Lebanese

Role of SN1 lipases on plasma lipids in metabolic syndrome and obesity.

Verónica Miksztowicz et al.

Arteriosclerosis, thrombosis, and vascular biology, 34(3), 669-675 (2014-01-25)

To assess the phospholipase activity of endothelial (EL) and hepatic lipase (HL) in postheparin plasma of subjects with metabolic syndrome (MS)/obesity and their relationship with atherogenic and antiatherogenic lipoproteins. Additionally, to evaluate lipoprotein lipase (LPL) and HL activity as triglyceride

Biocatalyzed Synthesis of Flavor Esters and Polyesters: A Design of Experiments (DoE) Approach.

Filippo Fabbri et al.

International journal of molecular sciences, 22(16) (2021-08-28)

In the present work, different hydrolases were adsorbed onto polypropylene beads to investigate their activity both in short-esters and polyesters synthesis. The software MODDE® Pro 13 (Sartorius) was used to develop a full-factorial design of experiments (DoE) to analyse the

In vitro inhibitory effects of Limonium contortirameum and L. virgatum extracts from sardinia on alpha-amylase, alpha-glucosidase and pancreatic lipase.

Marzia Foddai et al.

Natural product communications, 9(2), 181-184 (2014-04-03)

Pancreatic triacylglycerol lipase (PL), alpha-amylase and alpha-glucosidase are interesting pharmacological targets for the management of dyslipidemia, atherosclerosis, and obesity-diabetes. Limonium spp (Plumbaginaceae) are endemic to Sardinia, Italy. Comparable with acarbose, aqueous extracts (AE) of L. contortirameum and L. virgatum, and

Human milk bile-salt stimulated lipase. Sequence similarity with rat lysophospholipase and homology with the active site region of cholinesterases.

D L Christie et al.

FEBS letters, 278(2), 190-194 (1991-01-28)

To determine the active site residue, human milk bile-salt stimulated lipase (BSSL) was labelled with [3H]diisopropyl fluorophosphate (DFP). Partial sequence analysis of cyanogen bromide fragments (a total of 146 residues from 6 peptides) revealed 84% sequence identity with a putative

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