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David M Gau et al.
Bio-protocol, 10(8) (2020-07-01)
Kinases function as regulators of many cellular processes such as cell migration. These enzymes typically phosphorylate target motif sequences. Mass spec or phospho-specific antibody detection can be used to determine whether a kinase can phosphorylate proteins of interest, however, mass
Jingkai Zhou et al.
MethodsX, 8, 101517-101517 (2021-11-11)
Production of a protein of interest in bacteria and its purification from bacterial lysates are valuable tools for the purification of larger amounts of recombinant proteins. The low cost of culturing, and the rapid cell growth of bacteria make this
Natasa Anastasov et al.
Journal of hematopathology, 2(1), 9-19 (2009-08-12)
RNA interference is a powerful tool for the functional analysis of proteins by specific gene knockdown. In this study, we devised a rapid and efficient way to screen suitable siRNA sequences and subsequently employ them for specific gene knockdown in
Daniel Del Valle Morales et al.
Bio-protocol, 10(20), e3791-e3791 (2021-03-05)
The 5' cap is a ubiquitous feature of eukaryotic mRNAs. It is added in the nucleus onto newly synthesized pre-mRNA, and in the cytoplasm onto mRNAs after decapping or endonuclease cleavage. Cytoplasmic recapping can occur after loss of the cap
Genome-scale RNAi screens for high-throughput phenotyping in bloodstream-form African trypanosomes
Glover L, et al.
Nature Protocols, 10(1), 106-106 (2015)
Viscoelastic properties of vimentin originate from nonequilibrium conformational changes
Block J, et al.
Science advances, 4(6) (2018)
Linfeng Xu et al.
Nature communications, 12(1), 6803-6803 (2021-11-25)
Enzymes are represented across a vast space of protein sequences and structural forms and have activities that far exceed the best chemical catalysts; however, engineering them to have novel or enhanced activity is limited by technologies for sensing product formation.
Viscoelastic properties of vimentin originate from nonequilibrium conformational changes
Block J, et al.
Science Advances, 4(6) (2018)
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