the amount of
cobra venom anti-complementary protein, which causes
50% inhibition of lysis of 2 x 108 antibody sensitized
sheep erythrocytes (Product No. E9383) when
incubated with human complement serum
Growth Factor Magnetic Bead Panel
Catalog# MAGPMAG-24K
Control Catalog # MAGP-6024 LOT# MAGP-108 and MAGP-208
Additional Analytes on Reverse Side
Note: The Quality Control Ranges are generated
Bead Panel
Catalog # FCYTMAG-20K-PMX and FCYTMAG20KPX19BK
Control Catalog # FCY-6020 Lot # FCY-108 and FCY-208
Note: The Quality Control Ranges are generated using serum matrix provided in the kit
defined as
the amount of cobra venom anti-complementary protein
which causes 50% inhibition of lysis 2 x 108 antibody
sensitized sheep erythrocytes (Sigma Product No.
E7509) when incubated with human complement
concentration of approximately 5 µg/ml
complement C1q with GVB2+.
6. Prepare a suspension of 1.5 x 108 cells/ml of
antibody sensitized sheep erythrocytes (Product
No. E9383, EA7S) in GVB2+.
7. Prepare
1250000.0
lu
m
in
es
en
ce
104 105 106 107 108 109 1010
0.0
250000.0
500000.0
750000.0
1000000.0
1250000.0
lu
m
in
es
en
ce
104 105 106 107 108 109 1010
Cytokine Magnetic Bead Panel HSTC384-28K or HSTCMAG384-PX21
Control Catalog # HSTC-6028 Lot # HST-108 and HST-208
Note: The Quality Control Ranges are generated using serum matrix provided in the kit
1250000.0
lu
m
in
e
s
e
n
c
e
104 105 106 107 108 109 1010-5 -4 -3 -2 -1 0 1 2
0.0
250000.0
500000.0
750000.0
1000000.0
1250000.0
lu
m
in
e
s
e
n
c
e
104 105 106 107 108
spectrophotometer.
10. Calculate the final volume required to adjust the cell
concentration to 1 × 108 cells/ml:
A 415 of sample
A415 value given × Volume (A) = Volume (B)
A415 of sample – value
β-mercaptoethanol to 14ml of denaturating solution per
108 cells. This solution can be stored one month at room temperature.
2. Add 10ml of solution D per 108 cells. Mix thoroughly and allow to sit 1 min at
Catalog #PRCYT2MAG40K, PRCYTMAG40K-PX24, and PRCYTMAG40K-PX25
Control Catalog # PRCYT-6040 Lot # PRCYT-108 and PRCYT-208
***Additional Analytes on reverse side***
Note: The Quality Control Ranges are generated
(17), 3303-3310 (1972).
9. Naninga, L. B., and Guest, M. M., Arch, Biochem.
Biophys., 108, 542 (1964).
10. Ozawa, K., and Laskowski, M. Jr., The reactive
site of trypsin inhibitors. J. Biol. Chem
exact value on
lot-specific Certificate of Analysis)
• Particles per mL: ~5 × 108
• Particles per mg: ~5 × 108
Storage/Stability
Store this product at 2-8 °C for long-term