M5500

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[Molecular polymorphism of alpha-galactosidase MEL genes of Saccharomyces yeasts ].

E S Naumova et al.

Mikrobiologiia, 80(4), 496-507 (2011-11-12)

Characterization of Buckwheat Beverages Fermented with Lactic Acid Bacterial Cultures and Bifidobacteria.

Ewa Kowalska et al.

Foods (Basel, Switzerland), 9(12) (2020-12-03)

This study aimed to examine the effect of four different industrial starter cultures containing lactic acid bacteria (LAB) and bifidobacteria on selected characteristics of beverages prepared from buckwheat and stored at 4 °C for 28 days. This study included the

Studying substrate binding to reconstituted secondary transporters by attenuated total reflection infrared difference spectroscopy.

Víctor A Lórenz-Fonfría et al.

Methods in molecular biology (Clifton, N.J.), 914, 107-126 (2012-09-15)

The determination of protein conformational changes induced by the interaction of substrates with secondary transporters is an important step toward the elucidation of their transport mechanism. Since conformational changes in a protein alter its vibrational patterns, they can be detected

Lactulose and Melibiose Attenuate MPTP-Induced Parkinson's Disease in Mice by Inhibition of Oxidative Stress, Reduction of Neuroinflammation and Up-Regulation of Autophagy.

Chih-Hsin Lin et al.

Frontiers in aging neuroscience, 12, 226-226 (2020-08-28)

Parkinson's disease (PD) is a common neurodegenerative disease characterized by the progressive loss of dopaminergic (DAergic) neurons in the ventral brain. A disaccharide trehalose has demonstrated the potential to mitigate the DAergic loss in disease models for PD. However, trehalose

Comprehensive quality evaluation and comparison of Angelica sinensis radix and Angelica acutiloba radix by integrated metabolomics and glycomics.

Shan-Shan Zhou et al.

Journal of food and drug analysis, 26(3), 1122-1137 (2018-07-07)

Angelica radix (Danggui in Chinese) used in China and Japan is derived from two species of Angelica, namely Angelica sinensis and Angelica acutiloba, respectively. The differences in quality between A. sinensis radix (ASR) and A. acutiloba radix (AAR) should be

Diversity of the metabolic profiles of a broad range of lactic acid bacteria in soy juice fermentation.

Olivier Harlé et al.

Food microbiology, 89, 103410-103410 (2020-03-07)

This study explores the ability of lactic acid bacteria (LAB) to ferment soy juice. The ability of 276 LAB strains from 25 species to ferment the principal soy carbohydrates, sucrose, raffinose or stachyose was tested in synthetic media and a

Efficiency Analysis and Mechanism Insight of that Whole-Cell Biocatalytic Production of Melibiose from Raffinose with Saccharomyces cerevisiae.

Yingbiao Zhou et al.

Applied biochemistry and biotechnology, 181(1), 407-423 (2016-09-01)

Melibiose is widely used as a functional carbohydrate. Whole-cell biocatalytic production of melibiose from raffinose could reduce its cost. However, characteristics of strains for whole-cell biocatalysis and mechanism of such process are unclear. We compared three different Saccharomyces cerevisiae strains

Biocatalytic Production of Amino Carbohydrates through Oxidoreductase and Transaminase Cascades.

Ville Aumala et al.

ChemSusChem, 12(4), 848-857 (2018-12-28)

Plant-derived carbohydrates are an abundant renewable resource. Transformation of carbohydrates into new products, including amine-functionalized building blocks for biomaterials applications, can lower reliance on fossil resources. Herein, biocatalytic production routes to amino carbohydrates, including oligosaccharides, are demonstrated. In each case

Lactulose and Melibiose Inhibit α-Synuclein Aggregation and Up-Regulate Autophagy to Reduce Neuronal Vulnerability.

Chiung Mei Chen et al.

Cells, 9(5) (2020-05-21)

Parkinson's disease (PD) is a neurodegenerative disease characterized by selective dopaminergic (DAergic) neuronal degeneration in the substantia nigra (SN) and proteinaceous α-synuclein-positive Lewy bodies and Lewy neuritis. As a chemical chaperone to promote protein stability and an autophagy inducer to

A novel β-glucosidase from Saccharophagus degradans 2-40T for the efficient hydrolysis of laminarin from brown macroalgae.

Dong Hyun Kim et al.

Biotechnology for biofuels, 11, 64-64 (2018-03-23)

Laminarin is a potential biomass feedstock for the production of glucose, which is the most preferable fermentable sugar in many microorganisms by which it can be converted to biofuels and bio-based chemicals. Also, laminarin is a good resource as functional

Evaluation of the impact of a rat small intestinal extract on the digestion of four different functional fibers.

Pablo Gallego-Lobillo et al.

Food & function, 11(5), 4081-4089 (2020-04-26)

The degree of digestion, modulated by rat small intestinal extract on different functional fibers was investigated. In general, inulin-type fructans and fructooligosaccharides were the most resistant to the enzymatic digestion. Results evidenced the high-resistance of fructosyl-fructose bonds. This fits well

Phase diagrams of monoacylated amide-linked disaccharide glycolipids.

S Gerber et al.

Chemistry and physics of lipids, 158(2), 118-130 (2009-05-12)

A series of monoacylated glycolipids with even-numbered acyl chain lengths ranging from saturated C11 to C15 and an unsaturated C17:1 fatty acid connected by an amide in linkage to the disaccharide head groups maltose, melibiose and lactose were synthesized. The

Structure-activity relationship of three synthesized benzimidazole-based oligosaccharides in human platelet activation.

Yi Chang et al.

International journal of molecular medicine, 40(5), 1520-1528 (2017-09-28)

Antiplatelet agents have considerable benefits in the treatment of thromboembolic diseases; however, these agents still have substantial limitations due to their severe side-effects. In this study, the antiplatelet activity of three newly synthesized saccharide based benzimidazole derivatives, M3BIM, Malto-BIM and

The melibiose/Na+ symporter of Escherichia coli: kinetic and molecular properties.

T Pourcher et al.

Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 326(1236), 411-423 (1990-01-30)

The role of the co-transported cation in the coupling mechanism of the melibiose permease of Escherichia coli has been investigated by analysing its sugar-binding activity, facilitated diffusion reactions and energy-dependent transport reactions catalysed by the carrier functioning either as an

Introduction of H-antigens into oligosaccharides and sugar chains of glycoproteins using highly efficient 1,2-α-l-fucosynthase.

Yuta Sugiyama et al.

Glycobiology, 26(11), 1235-1247 (2016-08-24)

Fucα1-2 Gal linkages, or H-antigens, constitute histo-blood group antigens and are involved in various physiological processes. In addition, recent studies have shown that the H-antigen-containing glycans play an important role, not only in establishing harmonious relationship between gut microbes and

Expanded metabolomics approach to profiling endogenous carbohydrates in the serum of ovarian cancer patients.

Yu Cheng et al.

Journal of separation science, 39(2), 316-323 (2015-11-10)

We applied hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry to the quantitative analysis of serum from 58 women, including ovarian cancer patients, ovarian benign tumor patients, and healthy controls. All of these ovarian cancer and ovarian benign tumor

Are melibiose-fermenting intestinal and alpechin strains of Saccharomyces cerevisiae a novel type of yeast probiotics?

G I Naumov

Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections, 439, 262-263 (2011-09-29)

Comparative metabolomic analysis of Crypthecodinium cohnii in response to different dissolved oxygen levels during docosahexaenoic acid fermentation.

Jinjin Diao et al.

Biochemical and biophysical research communications, 499(4), 941-947 (2018-04-08)

Oxygen supply is an important factor during Crypthecodinium cohnii fermentation for docosahexaenoic acid (DHA) production. However, few studies about the intrinsic correlation between dissolved oxygen (DO) and cellular metabolism have been reported. In this study, the responses of C. cohnii to

[Structural analysis of genes participating in melibiose fermentation and isocitrate lyase production in Yersinia pestis strains of main and non main subspecies].

G A Eroshenko et al.

Genetika, 47(10), 1328-1334 (2012-01-12)

Comparative analysis of nucleotide sequences of genes participating in melibiose fermentation and isocitrate lyase production was conducted in 90 natural Yersinia pestis strains of main and non main subspecies. It was ascertained that the lack of the ability to utilize

Functional analysis of family GH36 α-galactosidases from Ruminococcus gnavus E1: insights into the metabolism of a plant oligosaccharide by a human gut symbiont.

M Cervera-Tison et al.

Applied and environmental microbiology, 78(21), 7720-7732 (2012-08-28)

Ruminococcus gnavus belongs to the 57 most common species present in 90% of individuals. Previously, we identified an α-galactosidase (Aga1) belonging to glycoside hydrolase (GH) family 36 from R. gnavus E1 (M. Aguilera, H. Rakotoarivonina, A. Brutus, T. Giardina, G.

Biosynthesis of Nondigestible Galactose-Containing Hetero-oligosaccharides by Lactobacillus plantarum WCFS1 MelA α-Galactosidase.

Paloma Delgado-Fernandez et al.

Journal of agricultural and food chemistry, 69(3), 955-965 (2021-01-13)

This work describes the high capacity of MelA α-galactosidase from Lactobacillus plantarum WCFS1 to transfer galactosyl residues from melibiose to the C6-hydroxyl group of disaccharide-acceptors with β-linkages (lactulose, lactose, and cellobiose) or α-linkages (isomaltulose and isomaltose) to produce novel galactose-containing

Biochemical characterization of a novel thermophilic α-galactosidase from Talaromyces leycettanus JCM12802 with significant transglycosylation activity.

Caihong Wang et al.

Journal of bioscience and bioengineering, 121(1), 7-12 (2015-06-20)

Thermophilic α-galactosidases have great potentials in biotechnological and medicinal applications due to their high-temperature activity and specific stability. In this study, a novel α-galactosidase gene of glycoside hydrolase family 27 (aga27A) was cloned from Talaromyces leycettanus JCM12802 and successfully expressed

The substitution of Arg149 with Cys fixes the melibiose transporter in an inward-open conformation.

Yibin Lin et al.

Biochimica et biophysica acta, 1828(8), 1690-1699 (2013-03-19)

The melibiose transporter from Escherichia coli (MelB) can use the electrochemical energy of either H(+), Na(+) or Li(+) to transport the disaccharide melibiose to the cell interior. By using spectroscopic and biochemical methods, we have analyzed the role of Arg149

Two aspects of honeydew honey authenticity: Application of advance analytical methods and chemometrics.

Vesna Vasić et al.

Food chemistry, 305, 125457-125457 (2019-09-11)

Taking into account a growing market and small number of articles related to honeydew honey, a metabolomic approach associated with multivariate analysis and modelling was proposed to discriminate five varieties of honey. Advanced analytical techniques were used for determination of

Optimization and Validation of an HPAEC-PAD Method for the Quantification of FODMAPs in Cereals and Cereal-Based Products.

Lilit Ispiryan et al.

Journal of agricultural and food chemistry, 67(15), 4384-4392 (2019-03-28)

This study presents an analytical method for the quantification of fermentable oligo-, di-, and monosaccharides and polyols (FODMAPs) in cereals and cereal-based products, considering diverse ingredients, such as different cereals in addition to wheat, pulses, or pseudocereals. All carbohydrates have

Embryonic Epidermal Lectins in Three Amphibian Species, Rana ornativentris, Bufo japonicus formosus, and Cynops pyrrhogaster.

Saburo Nagata et al.

Zoological science, 37(4), 338-345 (2020-07-31)

Intelectins (Itlns) are secretory lectins found in several chordate species that recognize carbohydrates on the bacterial cell surface depending on Ca2+ . In newly hatched larvae of Rana ornativentris (R. orn), Bufo japonicus formosus (B. jpn), and Cynops pyrrhogaster (C.

Purification and characterization of Aspergillus terreus α-galactosidases and their use for hydrolysis of soymilk oligosaccharides.

Joana Gasperazzo Ferreira et al.

Applied biochemistry and biotechnology, 164(7), 1111-1125 (2011-02-19)

α-Galactosidases has the potential to hydrolyze α-1-6 linkages in raffinose family oligosaccharides (RFO). Aspergillus terreus cells cultivated on wheat bran produced three extracellular forms of α-galactosidases (E1, E2, and E3). E1 and E2 α-galactosidases presented maximal activities at pH 5

A C-type lectin isolated from the skin of Japanese bullhead shark (Heterodontus japonicus) binds a remarkably broad range of sugars and induces blood coagulation.

Shigeyuki Tsutsui et al.

Journal of biochemistry, 157(5), 345-356 (2014-12-01)

The aim of this study was to determine the physiological role of skin lectins of the Japanese bullhead shark (Heterodontus japonicus). A skin extract was subjected to affinity chromatography using seven different sugars as ligands. Molecular mass and N-terminal amino

Internalization of a novel, huge lectin from Ibacus novemdentatus (slipper lobster) induces apoptosis of mammalian cancer cells.

Y Fujii et al.

Glycoconjugate journal, 34(1), 85-94 (2016-09-24)

An N-acetyl sugar-binding lectin (termed iNoL) displaying cytotoxic activity against human cancer cells was isolated from the slipper lobster Ibacus novemdentatus (family Scyllaridae). iNoL recognized monosaccharides containing N-acetyl group, and glycoproteins (e.g., BSM) containing oligosaccharides with N-acetyl sugar. iNoL was

Metabolism of the predominant human milk oligosaccharide fucosyllactose by an infant gut commensal.

Kieran James et al.

Scientific reports, 9(1), 15427-15427 (2019-10-30)

A number of bifidobacterial species are found at a particularly high prevalence and abundance in faecal samples of healthy breastfed infants, a phenomenon that is believed to be, at least partially, due to the ability of bifidobacteria to metabolize Human

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