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M5500
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Shigeyuki Tsutsui et al.
Journal of biochemistry, 157(5), 345-356 (2014-12-01)
The aim of this study was to determine the physiological role of skin lectins of the Japanese bullhead shark (Heterodontus japonicus). A skin extract was subjected to affinity chromatography using seven different sugars as ligands. Molecular mass and N-terminal amino
Joana Gasperazzo Ferreira et al.
Applied biochemistry and biotechnology, 164(7), 1111-1125 (2011-02-19)
α-Galactosidases has the potential to hydrolyze α-1-6 linkages in raffinose family oligosaccharides (RFO). Aspergillus terreus cells cultivated on wheat bran produced three extracellular forms of α-galactosidases (E1, E2, and E3). E1 and E2 α-galactosidases presented maximal activities at pH 5
Chiung Mei Chen et al.
Cells, 9(5) (2020-05-21)
Parkinson's disease (PD) is a neurodegenerative disease characterized by selective dopaminergic (DAergic) neuronal degeneration in the substantia nigra (SN) and proteinaceous α-synuclein-positive Lewy bodies and Lewy neuritis. As a chemical chaperone to promote protein stability and an autophagy inducer to
Saburo Nagata et al.
Zoological science, 37(4), 338-345 (2020-07-31)
Intelectins (Itlns) are secretory lectins found in several chordate species that recognize carbohydrates on the bacterial cell surface depending on Ca2+ . In newly hatched larvae of Rana ornativentris (R. orn), Bufo japonicus formosus (B. jpn), and Cynops pyrrhogaster (C.
The melibiose permease of Escherichia coli: importance of the NH2-terminal domains for cation recognition by the Na+/sugar cotransporter.
G Leblanc et al.
Society of General Physiologists series, 48, 213-227 (1993-01-01)
T E Arsen'eva et al.
Zhurnal mikrobiologii, epidemiologii, i immunobiologii, (4)(4), 75-81 (2010-08-28)
To characterize species specificity of officially recommended tests for differentiation of Yersiniapestis and Yersinia pseudotuberculosis and propose additional tests allowing for more accurate identification. Natural, laboratory and typical strains oftwo Yersinia species were studied using microbiological, molecular and biochemical methods.
Sanjivani B Gajdhane et al.
3 Biotech, 6(2), 260-260 (2016-01-01)
Response surface methodology was used to enhance the production of α-galactosidase from Fusarium moniliforme NCIM 1099 in solid-state fermentation. Plackett-Burman design was employed for selection of critical media constituents which were optimized by central composite rotatable design. Wheat bran, peptone
Paloma Delgado-Fernandez et al.
Journal of agricultural and food chemistry, 69(3), 955-965 (2021-01-13)
This work describes the high capacity of MelA α-galactosidase from Lactobacillus plantarum WCFS1 to transfer galactosyl residues from melibiose to the C6-hydroxyl group of disaccharide-acceptors with β-linkages (lactulose, lactose, and cellobiose) or α-linkages (isomaltulose and isomaltose) to produce novel galactose-containing
Chih-Hsin Lin et al.
Frontiers in aging neuroscience, 12, 226-226 (2020-08-28)
Parkinson's disease (PD) is a common neurodegenerative disease characterized by the progressive loss of dopaminergic (DAergic) neurons in the ventral brain. A disaccharide trehalose has demonstrated the potential to mitigate the DAergic loss in disease models for PD. However, trehalose
Priya Voothuluru et al.
Frontiers in plant science, 11, 392-392 (2020-05-07)
Reactive oxygen species (ROS) can act as signaling molecules involved in the acclimation of plants to various abiotic and biotic stresses. However, it is not clear how the generalized increases in ROS and downstream signaling events that occur in response
Roberta Polak et al.
Advanced healthcare materials, 4(18), 2832-2841 (2015-12-01)
Cell backpacks, or micron-scale patches of a few hundred nanometers in thickness fabricated by layer-by-layer (LbL) assembly, are potentially useful vehicles for targeted drug delivery on the cellular level. In this work, echogenic liposomes (ELIPs) containing the anticancer drug doxorubicin
Jo Sing Julia Tang et al.
Molecules (Basel, Switzerland), 24(10) (2019-05-18)
Interactions between glycans and proteins have tremendous impact in biomolecular interactions. They are important for cell-cell interactions, proliferation and much more. Here, we emphasize the glycan-mediated interactions between pathogens and host cells. Pseudomonas aeruginosa, responsible for a huge number of
Mohamed S Elrobh et al.
FEMS microbiology letters, 338(1), 62-67 (2012-10-17)
The Escherichia coli melR gene encodes the MelR transcription factor that controls melibiose utilization. Expression of melR is autoregulated by MelR, which represses the melR promoter by binding to a target that overlaps the transcript start. Here, we show that
Xiu-Zhen Shi et al.
Insect biochemistry and molecular biology, 52, 102-114 (2014-07-12)
C-type lectins (CTLs) are a superfamily of calcium-dependent carbohydrate binding proteins containing at least one carbohydrate-recognition domain (CRD) and they are present in almost all metazoans. Insect CTLs may function as pattern-recognition receptors and play important roles in innate immunity.
Y Fujii et al.
Glycoconjugate journal, 34(1), 85-94 (2016-09-24)
An N-acetyl sugar-binding lectin (termed iNoL) displaying cytotoxic activity against human cancer cells was isolated from the slipper lobster Ibacus novemdentatus (family Scyllaridae). iNoL recognized monosaccharides containing N-acetyl group, and glycoproteins (e.g., BSM) containing oligosaccharides with N-acetyl sugar. iNoL was
Kieran James et al.
Scientific reports, 9(1), 15427-15427 (2019-10-30)
A number of bifidobacterial species are found at a particularly high prevalence and abundance in faecal samples of healthy breastfed infants, a phenomenon that is believed to be, at least partially, due to the ability of bifidobacteria to metabolize Human
L Zhang et al.
Prikladnaia biokhimiia i mikrobiologiia, 48(2), 243-248 (2012-05-17)
The gene mel1, encoding alpha-galactosidase in Schizosaccharomyces pombe, and the gene bgl2, encoding and beta-glucosidase in Trichoderma reesei, were isolated and co-expressed in the industrial ethanol-producing strain of Saccharomyces cerevisiae. The resulting strains were able to grow on cellobiose and
Víctor A Lórenz-Fonfría et al.
Methods in molecular biology (Clifton, N.J.), 914, 107-126 (2012-09-15)
The determination of protein conformational changes induced by the interaction of substrates with secondary transporters is an important step toward the elucidation of their transport mechanism. Since conformational changes in a protein alter its vibrational patterns, they can be detected
Shan-Shan Zhou et al.
Journal of food and drug analysis, 26(3), 1122-1137 (2018-07-07)
Angelica radix (Danggui in Chinese) used in China and Japan is derived from two species of Angelica, namely Angelica sinensis and Angelica acutiloba, respectively. The differences in quality between A. sinensis radix (ASR) and A. acutiloba radix (AAR) should be
Kristin R McKenna et al.
The Analyst, 145(24), 8008-8015 (2020-10-15)
Carbohydrates are the most abundant class of biomolecules on Earth with a diverse array of biological functions. It is hypothesized that they likely had an important role in the development of life on the primoridal Earth as well. Since sugars
Caihong Wang et al.
Journal of bioscience and bioengineering, 121(1), 7-12 (2015-06-20)
Thermophilic α-galactosidases have great potentials in biotechnological and medicinal applications due to their high-temperature activity and specific stability. In this study, a novel α-galactosidase gene of glycoside hydrolase family 27 (aga27A) was cloned from Talaromyces leycettanus JCM12802 and successfully expressed
Jin Myong Jang et al.
International journal of biological macromolecules, 131, 1138-1146 (2019-04-15)
Raffinose family oligosaccharides (RFOs) negatively affect nutritional value of legume-derived food and feed. It has been challenging to develop a high performance α-galactosidase excelled on catalytic efficiency, thermostability, pH stability and protease-resistance that could efficiently hydrolyze RFOs. In this study
G A Eroshenko et al.
Genetika, 47(10), 1328-1334 (2012-01-12)
Comparative analysis of nucleotide sequences of genes participating in melibiose fermentation and isocitrate lyase production was conducted in 90 natural Yersinia pestis strains of main and non main subspecies. It was ascertained that the lack of the ability to utilize
M Cervera-Tison et al.
Applied and environmental microbiology, 78(21), 7720-7732 (2012-08-28)
Ruminococcus gnavus belongs to the 57 most common species present in 90% of individuals. Previously, we identified an α-galactosidase (Aga1) belonging to glycoside hydrolase (GH) family 36 from R. gnavus E1 (M. Aguilera, H. Rakotoarivonina, A. Brutus, T. Giardina, G.
Jinjin Diao et al.
Biochemical and biophysical research communications, 499(4), 941-947 (2018-04-08)
Oxygen supply is an important factor during Crypthecodinium cohnii fermentation for docosahexaenoic acid (DHA) production. However, few studies about the intrinsic correlation between dissolved oxygen (DO) and cellular metabolism have been reported. In this study, the responses of C. cohnii to
Lilit Ispiryan et al.
Journal of agricultural and food chemistry, 67(15), 4384-4392 (2019-03-28)
This study presents an analytical method for the quantification of fermentable oligo-, di-, and monosaccharides and polyols (FODMAPs) in cereals and cereal-based products, considering diverse ingredients, such as different cereals in addition to wheat, pulses, or pseudocereals. All carbohydrates have
Yuta Sugiyama et al.
Glycobiology, 26(11), 1235-1247 (2016-08-24)
Fucα1-2 Gal linkages, or H-antigens, constitute histo-blood group antigens and are involved in various physiological processes. In addition, recent studies have shown that the H-antigen-containing glycans play an important role, not only in establishing harmonious relationship between gut microbes and
Are melibiose-fermenting intestinal and alpechin strains of Saccharomyces cerevisiae a novel type of yeast probiotics?
G I Naumov
Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections, 439, 262-263 (2011-09-29)
Yingbiao Zhou et al.
Applied biochemistry and biotechnology, 181(1), 407-423 (2016-09-01)
Melibiose is widely used as a functional carbohydrate. Whole-cell biocatalytic production of melibiose from raffinose could reduce its cost. However, characteristics of strains for whole-cell biocatalysis and mechanism of such process are unclear. We compared three different Saccharomyces cerevisiae strains
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