the analysis of stained slides
with a microscopic magnification >40x.
Staining of histological samples
Staining in the staining cell
Deparaffinize histological slides in the conventional manner
to 60 to 70 °C for 20 minutes.
4. Remove the slides from the oven and allow them to
reach room temperature. The slides are now ready
for microscopic visualization.
Notes:
• Do not
Visualizing Membranes on Microscope Slides (for higher magnification or
with objectives with short working distances)
The membrane can be removed from each well for microscopic evaluation. This allows
15 min
allow to run off thoroughly, dabbing the slide dry if necessary
mount the slides with e.g. Aquatex® and cover glass
microscope under UV excitation
Microscopy
4’,6-Diamidino-2-phenylindole
pre-stained before loading into the slide.
3. Can I use a normal digital camera
connected to a microscope to take
images manually at different time
points (e.g. if the microscope doesn’t
have video equipment
is
applied onto the slide next to the white printed labelling field. One slide is
already stained with the reference method and is used for comparison. The
24 unstained slides are stained according
to 80 °C for 15 to 30 min.
Remove the slides from the oven and allow them to
cool to room temperature. The slides are now
ready for microscopic visualization.
Notes:
• Some tissue
recover in the dark for 2 hours.
Store slides in 4 °C.
6b. Microscope Observation
Observe under microscope. If the cells
on slide appear too dense/far apart, repeat
the steps with higher/lower dilution
of stained slides
with a microscopic magnification >40x.
Result
Nuclei dark blue
Tissue blue
Procedure - Metachromasia
Staining in the staining cell
Deparaffinize histological slides in the conventional
can then be stored.
The use of immersion oil is recommended for the analysis of stained slides with a
microscopic magnification >40x.
Result
Buffer solution Buffer solution Buffer solution
pH 6.4
for the analysis of stained slides
with a microscopic magnification >40x.
Staining of histological samples
Staining in the staining cell
Deparaffinize histological slides in the conventional manner
recover in the dark for 2 hours.
Store slides in 4 °C.
6b. Microscope Observation
Observe under microscope. If the cells
on slide appear too dense/far apart, repeat
the steps with higher/lower dilution
analysis of stained slides
with a microscopic magnification >40x.
Result
Free iron (Fe3+) intensive blue granules
Nuclei pale red
Cytoplasm tender pink
Technical notes
The microscope used should meet
concentration of 5 x 10 cells/mL in PBS are pipetted dropwise on PTFE- coated printed microscope slides containing ten 5
mM wells/slide. After the cells are allowed to settle to the surface of the glass (10-15
clean, glass microscope slides and allow
to dry at room temperature (25 °C) for 5-16 hours.
6. Immerse the slides in the pre-cooled acetone
(−20 °C) for 20 minutes.
7. Allow slides to dry briefly
tissue is
applied onto the slide next to the white printed labelling field. One slide is
already stained with the reference method and is used for comparison.The
24 unstained slides are stained according
clean, glass microscope slides and allow
to dry at room temperature (25 °C) for 5-16 hours.
6. Immerse the slides in the pre-cooled acetone
(−20 °C) for 20 minutes.
7. Allow slides to dry briefly
concentration of 5 x 106 cells/mL in PBS are pipetted dropwise on PTFE- coated printed microscope slides containing ten
5 mm wells/slide. After the cells are allowed to settle to the surface of the glass (10-15 minutes
in a fume hood.
The slides are mounted by dropping approx. 0.2 ml of the listed mounting me-
dium onto the horizontal slide using a glass rod, which will fi ll the space between
slide and cover glass. As
analysis of stained slides
with a microscopic magnification >40x.
Result
The use of these aqueous, ready-to-use mounting media results in com-
pletely airtight specimen slides, the structure and stain
just a few hours.
The use of immersion oil is recommended for the analysis of stained slides with a
microscopic magnification >40x.
Result
Gram-positive microorganisms blue-violet
Gram-negative
labeled microscope slides, make thin blood smears. Pre pare CONTROL
slides using positive HbF blood (cord-blood) and normal adult blood. Air dry
approximately 10 minutes.
3. Fix slides by immersing
remove the excess
water. Alternatively, the slide may shaken to remove most of the water and air-dried.
9. Examine the finished slide under a microscope (oil immersion objective).
Attention: Wash
concentration of 5 x 106 cells/mL in PBS are pipetted dropwise on PTFE- coated printed microscope slides containing ten 5
mm wells/slide. After the cells are allowed to settle to the surface of the glass (10-15
for the analysis of stained slides with a
microscopic magnification >40x.
Result
Glycogen red
Mucous, fibrin, amyloid pink to red
Technical notes
The microscope used should meet the requirements
for just a few hours.
The use of immersion oil is recommended for the analysis of stained slides
with a microscopic magnification >40x.
Result
Acid-fast bacteria (AFB) red
Background light blue
analysis of stained slides
with a microscopic magnification >40x.
Result
The use of these aqueous, ready-to-use mounting media results in com-
pletely airtight specimen slides, the structure and stain