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关键词:'microscopic slides'
显示 1-30 共 218 条结果 关于 "microscopic slides" 范围 论文
Thomas Schmid et al.
Polymers, 13(13) (2021-07-03)
Microscope slide collections represent extremely valuable depositories of research material in a natural history, forensic, veterinary, and medical context. Unfortunately, most mounting media of these slides deteriorate over time, with the reason for this not yet understood at all. In
Jie Jiang et al.
Analytical chemistry, 87(16), 8057-8062 (2015-07-24)
Ambient ionization mass spectrometry is achieved in a simple manner by loading a sample solution onto a corner of a microscope cover glass positioned in front of the inlet to a mass spectrometer and applying a high voltage to the
Oscar Holmström et al.
PloS one, 10(12), e0144688-e0144688 (2015-12-15)
A significant barrier to medical diagnostics in low-resource environments is the lack of medical care and equipment. Here we present a low-cost, cloud-connected digital microscope for applications at the point-of-care. We evaluate the performance of the device in the digital
Meng M Rowland et al.
Nucleic acids research, 42(14), 9295-9303 (2014-07-14)
The DNA backbone is often considered a track that allows long-range sliding of DNA repair enzymes in their search for rare damage sites in DNA. A proposed exemplar of DNA sliding is human 8-oxoguanine ((o)G) DNA glycosylase 1 (hOGG1), which
Stella H North et al.
Analytical chemistry, 82(1), 406-412 (2009-12-17)
Glass microscope slides are considered by many as the substrate of choice for microarray manufacturing due to their amenability to various surface chemistry modifications. The use of silanes to attach various functional groups onto glass slides has provided a versatile
Marcia B C Mello et al.
Malaria journal, 13, 238-238 (2014-06-19)
Due to students' initial inexperience, slides are frequently broken and blood smears are damaged in microscopy training, leading to the need for their constant replacement. To minimize this problem a method of preparing blood smears on transparent acetate sheets was
Emmanuel I Odongo-Aginya et al.
African health sciences, 7(1), 33-36 (2007-07-03)
The Kato-Katz thick smear technique is the standard technique recommended by the World Health Organisation for the quantitative diagnosis of Schistosoma mansoni and other intestinal helminth infections. The major problem of the technique is that a few hours after the
Silvia Bonaccorsi et al.
Cold Spring Harbor protocols, 2012(8), doi:10-doi:10 (2012-08-03)
This protocol describes chromatin staining of Drosophila testes. To visualize DNA, preparations fixed using methanol-acetone, paraformaldehyde, or formaldehyde can be stained with several DNA-binding dyes. If the slides are to be examined with a fluorescence microscope equipped with filters that
Shabnam Abtahi et al.
Current protocols, 1(4), e68-e68 (2021-04-07)
High-throughput, high-content imaging technologies and multiplex slide scanning have become widely used. Advantages of these approaches include the ability to archive digital copies of slides, review slides as teams using virtual microscopy software, and standardize analytical approaches. The cost and
Cleopatra Kozlowski et al.
Toxicologic pathology, 46(3), 336-347 (2018-04-24)
The bone marrow is an important site for assessment of the hematopoietic toxicity of new drug candidates. Here, we extended our previous work, where we developed a computer algorithm to automatically quantitate overall bone marrow cell density by analyzing digitized
Hadi Mirahmadi et al.
Iranian journal of parasitology, 11(4), 489-498 (2017-01-28)
Leishmaniasis is a sand fly-borne disease caused by the protozoan parasites belonging to the genus A total of 130 Giemsa-Stained slides from suspected Cutaneous leishmaniasis (CL) patients were examined under a light microscope at ×1000. DNA from each slide was
K Wenzelides et al.
Acta histochemica, 69(2), 307-313 (1981-01-01)
The relative RNA-content of liver cell nuclei from histological slides of rat liver was determined by automatical microscope picture analysis. The slides were stained with Gallocyanin-Chromealum. Extinction measurements are made before and after perchloracid extraction of RNA for the same
G Danscher et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 31(12), 1394-1398 (1983-12-01)
RNase labeled with colloidal gold was used as a model for the present technique evolved for the light microscopic localization of gold-labeled substances in semithin resin-embedded sections. Tissue sections placed on glass slides were treated with the gold-enzyme complex and
Daniel E Campton et al.
BMC cancer, 15, 360-360 (2015-05-07)
Circulating tumor cells (CTCs) are malignant cells that have migrated from solid cancers into the blood, where they are typically present in rare numbers. There is great interest in using CTCs to monitor response to therapies, to identify clinically actionable
Philip Thomas et al.
Nature protocols, 4(6), 825-837 (2009-05-16)
The Buccal Micronucleus Cytome (BMCyt) assay is a minimally invasive method for studying DNA damage, chromosomal instability, cell death and the regenerative potential of human buccal mucosal tissue. This method is increasingly used in molecular epidemiological studies for investigating the
J L Perry et al.
Journal of clinical microbiology, 27(6), 1411-1412 (1989-06-01)
An opaque, water-insoluble quality control material with a skinlike microscopic appearance was prepared by inoculating melted xanthine (0.4%) agar with filamentous fungi and dispensing drops onto glass slides. After solidification of the agar, the material was rapidly cleared by 10%
Flávia T F Pacheco et al.
The Journal of parasitology, 99(6), 1002-1008 (2013-07-09)
Despite the availability of many parasitological methods for detection of Cryptosporidium and Isospora (Cystoisospora) belli in fecal samples, there are uncertainties about the accuracy of these techniques in laboratory practice. In this study, 27 formalin-fixed positive stool samples for Cryptosporidium
Alexander Zarkov et al.
Biotechnology, biotechnological equipment, 28(1), 112-117 (2014-01-02)
Here we propose an easy to build up and apply glass slide preparation system for single DNA molecules stretching. It is based on fast and simple coating of a solid glass with a cocktail of acrylic monomers that are easily
S C K Carlsen et al.
Chemosphere, 64(5), 787-794 (2005-12-13)
In the present study the evaporation of 10 herbicides was investigated during five field experiments, and the amount deposited per surface area was quantified inside the field using simple passive dosimeters consisting of microscope slides placed on plastic lids. On
Sen Xiang et al.
The Analyst, 137(7), 1669-1673 (2012-02-16)
In this work, two different deposition methods of 3-aminopropyltriethoxysilane (APTES) on glass slides were compared in order to study the adhesion effect of cervical exfoliated cells on smear slides. Glass slides were modified by vapor-phase deposition (V-D) and liquid-phase deposition
Yan-qiong Zhang et al.
[Zhonghua yan ke za zhi] Chinese journal of ophthalmology, 44(5), 448-454 (2008-10-29)
To study the development and opsin expression of zebrafish photoreceptors after CRX gene knock-down. It was a experimental study. CRX-MOs was microinjected into the zygote of zebrafish. Seventy-two and 96 hours post fertilization, we prepared the eye histological slides, electron
Huiyan Li et al.
Scientific reports, 5, 11688-11688 (2015-07-08)
The coordinated delivery of minute amounts of different reagents is important for microfluidics and microarrays, but is dependent on advanced equipment such as microarrayers. Previously, we developed the snap chip for the direct transfer of reagents, thus realizing fluidic operations
Taiga Uekusa et al.
Journal of pharmaceutical and biomedical analysis, 161, 45-50 (2018-08-26)
The purpose of the present study was to investigate the precipitation of a drug on the particle surface of its salt in biorelevant media. Pioglitazone (PIO, weak base, pKa = 5.8) was used as a model drug. The crystal particles of PIO
Luis Eduardo Silva Móz et al.
Inhalation toxicology, 25(1), 17-20 (2013-01-09)
To assess the behavior of the immunoexpression of protein p53 in Reinke's edema and laryngeal squamous cell carcinoma. retrospective. we recovered the histological paraffin blocks of patients who were subjected to Reinke's edema and laryngeal squamous cell carcinoma surgery in
T J Filler et al.
Histochemistry, 101(5), 375-378 (1994-06-01)
This paper introduces two innovations in reflection contrast microscopy (RCM): (1) an extended application for qualitative light microscopic investigations; and (2) a novel method for quantification in cytochemistry. (1) We found out that RCM cannot only be used for surface
B L Baisden et al.
The American journal of surgical pathology, 24(8), 1140-1146 (2000-08-10)
Despite the profound therapeutic and prognostic implications of nodal metastases in patients with melanoma, there is no consensus strategy for the optimal detection of metastases in sentinel lymph node biopsies. Traditional microscopic examination may be too crude to detect scattered
K Aaltonen et al.
British journal of cancer, 94(11), 1697-1702 (2006-05-04)
Cyclin A has in some studies been associated with poor breast cancer survival, although all studies have not confirmed this. Its prognostic significance in breast cancer needs evaluation in larger studies. Tissue microarray (TMA) technique allows a simultaneous analysis of
N Sandetskaya et al.
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 34(8), 1639-1645 (2015-05-20)
The molecular detection of microorganisms in liquid samples generally requires their enrichment or isolation. The aim of our study was to evaluate the capture and pre-concentration of bacteria by immobilized particular cationic antimicrobial peptides, called synthetic anti-lipopolysaccharide peptides (SALP). For
Andreas Toegl et al.
Journal of biomolecular techniques : JBT, 14(3), 197-204 (2003-09-19)
Protein and DNA microarrays have become a standard tool in proteomics/genomics research. In order to guarantee fast and reproducible hybridization results, the diffusion limit must be overcome. Surface acoustic wave (SAW) micro-agitation chips efficiently agitate the smallest sample volumes (down
Martin Bucher et al.
International journal of radiation biology, 96(10), 1263-1273 (2020-07-17)
In case of population exposure by ionizing radiation, a fast and reliable dose assessment of exposed and non-exposed individuals is crucial important. In initial triage, physicians have to take fast decisions whom to treat with adequate medical care. In addition
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