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关键词:'mouse embryonic fibroblasts'
显示 31-60 共 4629 条结果 关于 "mouse embryonic fibroblasts" 范围 论文
Haifeng Gu et al.
Nature communications, 10(1), 1465-1465 (2019-04-02)
Embryonic stem cells (ESCs) can propagate in an undifferentiated state indefinitely in culture and retain the potential to differentiate into any somatic lineage as well as germ cells. The catabolic process autophagy has been reported to be involved in ESC
Iyori Nojima et al.
Aging, 14(7), 2966-2988 (2022-04-05)
Insulin-like growth factor (IGF)-binding proteins (IGFBPs) are secretory proteins that regulate IGF signaling. In this study, we investigated the role of IGFBP5 in replicative senescence in embryonic mouse fibroblasts (MEFs). During passages according to the 3T3 method, MEFs underwent senescence
Sundararaghavan Pattabiraman et al.
Scientific reports, 10(1), 19525-19525 (2020-11-13)
Vimentin is one of the first cytoplasmic intermediate filaments to be expressed in mammalian cells during embryogenesis, but its role in cellular fitness has long been a mystery. Vimentin is acknowledged to play a role in cell stiffness, cell motility
Yonghee Song et al.
Biochemical and biophysical research communications, 503(1), 352-358 (2018-06-12)
Pluripotent embryonic stem cells are one of the best modalities for the disease treatment due to their potential for self-renewal and differentiation into various cell types. Induction of stem cell differentiation into specific cell lineages has been investigated for decades
Jiyoung Lee et al.
Nature communications, 11(1), 4283-4283 (2020-09-05)
Our understanding of the spatiotemporal regulation of cardiogenesis is hindered by the difficulties in modeling this complex organ currently by in vitro models. Here we develop a method to generate heart organoids from mouse embryonic stem cell-derived embryoid bodies. Consecutive
Irene Cantone et al.
Nature communications, 7, 12354-12354 (2016-08-11)
Erasure of epigenetic memory is required to convert somatic cells towards pluripotency. Reactivation of the inactive X chromosome (Xi) has been used to model epigenetic reprogramming in mouse, but human studies are hampered by Xi epigenetic instability and difficulties in
Aysegul Ors et al.
Biochemistry and cell biology = Biochimie et biologie cellulaire, 95(4), 491-499 (2017-02-09)
H3.3 is a histone variant that marks transcription start sites as well as telomeres and heterochromatic sites on the genome. The presence of H3.3 is thought to positively correlate with the transcriptional status of its target genes. Using a conditional
Bruno M Camargos et al.
Growth factors (Chur, Switzerland), 33(2), 133-138 (2014-11-22)
Activin A is a growth factor released by mature osteoblasts that has a critical effect on bone formation. We investigated the effect of bone morphogenetic protein (BMP)-4 on activin A gene expression during in vitro osteogenic differentiation of mouse embryonic
Constance S Petit et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(19), 10565-10574 (2020-04-30)
Numerous mutations that impair retrograde membrane trafficking between endosomes and the Golgi apparatus lead to neurodegenerative diseases. For example, mutations in the endosomal retromer complex are implicated in Alzheimer's and Parkinson's diseases, and mutations of the Golgi-associated retrograde protein (GARP)
Yanfeng Chen et al.
The international journal of biochemistry & cell biology, 57, 108-114 (2014-12-03)
Power frequency magnetic fields (PFMF) have been reported to affect several cellular functions, such as cell proliferation and apoptosis. In this study, we investigated the effects of PFMF on mouse embryonic fibroblasts (MEF) autophagy. After cells were exposed to 50
Zhen-Dong Xu et al.
Acta pharmacologica Sinica, 41(3), 303-310 (2019-10-28)
Propofol is a commonly used intravenous anesthetic agent, which has been found to affect cell survival and proliferation especially in early life. Our previous studies show that propofol-induced neurodegeneration and neurogenesis are closely associated with cell autophagy. In the present
Christian Schröter et al.
Development (Cambridge, England), 142(24), 4205-4216 (2015-10-30)
Intracellular transcriptional regulators and extracellular signaling pathways together regulate the allocation of cell fates during development, but how their molecular activities are integrated to establish the correct proportions of cells with particular fates is not known. Here we study this
David Colognori et al.
Molecular cell, 74(1), 101-117 (2019-03-05)
During X-inactivation, Xist RNA spreads along an entire chromosome to establish silencing. However, the mechanism and functional RNA elements involved in spreading remain undefined. By performing a comprehensive endogenous Xist deletion screen, we identify Repeat B as crucial for spreading Xist
Kun-Yong Kim et al.
Nature communications, 9(1), 2583-2583 (2018-07-04)
Embryonic stem cells (ESCs) maintain pluripotency through unique epigenetic states. When ESCs commit to a specific lineage, epigenetic changes in histones and DNA accompany the transition to specialized cell types. Investigating how epigenetic regulation controls lineage specification is critical in
Vivek Phani Varma et al.
PloS one, 10(7), e0131291-e0131291 (2015-07-03)
Cryostorage is of immense interest in biomedical research, especially for stem cell-based therapies and fertility preservation. Several protocols have been developed for efficient cryopreservation of cells and tissues, and a combination of dimethyl sulfoxide (DMSO) and fetal bovine serum (FBS)
Mohamed Al-Sayegh et al.
Adipocyte, 10(1), 1-20 (2020-12-22)
Our understanding of adipose tissue has progressed from an inert tissue for energy storage to be one of the largest endocrine organs regulating metabolic homoeostasis through its ability to synthesize and release various adipokines that regulate a myriad of pathways. The
Xin Xie et al.
Frontiers in immunology, 10, 836-836 (2019-05-07)
The activation of innate immunity by viral nucleic acids present in the cytoplasm plays an essential role in controlling viral infection in both immune and non-immune cells. The dsDNA and dsRNA viral mimics can stimulate the cytosolic nucleic acids sensors
Matteo Amitaba Molè et al.
Cell reports, 34(10), 108834-108834 (2021-03-11)
At implantation, the embryo establishes contacts with the maternal endometrium. This stage is associated with a high incidence of preclinical pregnancy losses. While the maternal factors underlying uterine receptivity have been investigated, the signals required by the embryo for successful
Xiang Lin et al.
International journal of biological sciences, 7(8), 1114-1121 (2011-09-20)
Although the fibroblast growth factor (FGF) signaling axis plays important roles in cell survival, proliferation, and differentiation, the molecular mechanism underlying how the FGF elicits these diverse regulatory signals is not well understood. By using the Frs2α null mouse embryonic
Hatice Burcu Şişli et al.
Methods in molecular biology (Clifton, N.J.) (2021-05-05)
Mouse embryonic stem cells (mESCs) were first derived and cultured nearly 30 years ago and have been beneficial tools to create transgenic mice and to study early mammalian development so far. Fibroblast feeder cell layers are often used at some stage
Huan Yang et al.
Cellular signalling, 75, 109732-109732 (2020-08-05)
The sigma-1 receptor (Sig1R) is a unique ligand-operated endoplasmic reticulum (ER) protein without any mammalian homolog. It has long been a pharmacological target for intervention of psychiatric disorders, and recently garnered refreshed interest for its neuroprotective potential. Though reported to
Daisuke Takekoshi et al.
In vitro cellular & developmental biology. Animal, 55(5), 355-367 (2019-04-18)
N-terminal acetylation (Nt-acetylation) refers to the acetylation of the free α-amino group at the N-terminus of a polypeptide. While the effects of Nt-acetylation are multifaceted, its most known function is in the acetylation-dependent N-end rule protein degradation pathway (Ac/N-end rule
Wei Chen et al.
Frontiers in genetics, 11, 867-867 (2020-08-28)
Replicative senescence is a hallmark of aging, which also contributes to individual aging. Mouse embryonic fibroblasts (MEFs) provide a convenient replicative senescence model. However, the heterogeneity of single MEFs during cellular senescence has remained unclear. Here, we conducted single-cell RNA
Maria Serra et al.
Development (Cambridge, England), 144(21), 3879-3893 (2017-09-28)
The
Marina Domingo-Vidal et al.
Molecular cancer research : MCR, 17(9), 1893-1909 (2019-06-27)
Head and neck squamous cell carcinoma (HNSCC) is comprised of metabolically linked distinct compartments. Cancer-associated fibroblasts (CAF) and nonproliferative carcinoma cells display a glycolytic metabolism, while proliferative carcinoma cells rely on mitochondrial oxidative metabolism fueled by the catabolites provided by
Huanhuan Li et al.
Cell stem cell, 20(4), 462-477 (2017-01-24)
RNA-binding proteins (RBPs), in addition to their functions in cellular homeostasis, play important roles in lineage specification and maintaining cellular identity. Despite their diverse and essential functions, which touch on nearly all aspects of RNA metabolism, the roles of RBPs
Anna De Gaetano et al.
Journal of clinical medicine, 9(6) (2020-06-12)
LONP1 is a nuclear-encoded mitochondrial protease crucial for organelle homeostasis; mutations of LONP1 have been associated with Cerebral, Ocular, Dental, Auricular, and Skeletal anomalies (CODAS) syndrome. To clarify the role of LONP1 in vivo, we generated a mouse model in
Jinglong Zhang et al.
Cell death & disease, 9(2), 224-224 (2018-02-16)
Unipotent spermatogonial stem cells (SSCs) can be efficiently reprogrammed into pluripotent stem cells only by manipulating the culture condition, without introducing exogenous reprogramming factors. This phenotype raises the hypothesis that the endogenous transcription factors (TFs) in SSCs may facilitate reprogramming
Zhenqiang Zhao et al.
Frontiers in cellular neuroscience, 10, 291-291 (2017-01-10)
Mouse embryonic fibroblasts (MEFs) and human foreskin fibroblasts (HFFs) are used for the culture of human embryonic stem cells (hESCs). MEFs and HFFs differed in their capacity to support the proliferation and pluripotency of hESCs and could affect cardiac differentiation
Tsutomu Motohashi et al.
Biology open, 5(3), 311-322 (2016-02-14)
Neural crest cells (NC cells) are multipotent cells that emerge from the edge of the neural folds and migrate throughout the developing embryo. Although the gene regulatory network for generation of NC cells has been elucidated in detail, it has
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