N1268

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Biocatalytic potential of Pseudolycoriella CAZymes (Sciaroidea, Diptera) in degrading plant and fungal cell wall polysaccharides.

Vitor Trinca et al.

iScience, 26(4), 106449-106449 (2023-04-07)

Soil biota has a crucial impact on soil ecology, global climate changes, and effective crop management and studying the diverse ecological roles of dipteran larvae deepens the understanding of soil food webs. A multi-omics study of Pseudolycoriella hygida comb. nov.

A novel cold-adapted and glucose-tolerant GH1 beta-glucosidase from Exiguobacterium antarcticum B7

Crespim E, et al.

International Journal of Biological Macromolecules, 82, 375-380 (2016)

A practical preparation of p-nitrophenyl beta-d-mannopyranoside

Rosenfield L and Yuan CL

Carbohydrate Research, 46(1), 155-158 (1976)

Synthesis of some D-mannosyl-oligosaccharides containing the methyl and 4-nitrophenyl beta-D-mannopyranoside units.

S H Khan et al.

Carbohydrate research, 207(1), 57-69 (1990-10-15)

Methyl 3,4,6-tri-O-benzyl-beta-D-mannopyranoside (2), methyl 2,3-O-isopropylidene-beta-D-mannopyranoside (11), and 4-nitrophenyl 2,3-O-isopropylidene-beta-D-mannopyranoside (12) were each condensed with 2,3,4,6-tetra-O-acetyl-alpha-D-mannopyranosyl bromide (1) in the presence of mercuric cyanide, to give after deprotection, methyl 2-(5) and 6-O-alpha-D-mannopyranosyl-beta-D-mannopyranoside (15), and 4-nitrophenyl 6-O-alpha-D-mannopyranosyl-beta-D-mannopyranoside (20), respectively. A similar condensation

In vitro and in vivo characterization of genes involved in mannan degradation in Neurospora crassa.

Yunhan Hsu et al.

Fungal genetics and biology : FG & B, 144, 103441-103441 (2020-08-11)

To better understand the roles of genes involved in mannan degradation in filamentous fungi, in this study we searched, identified, and characterized one putative GH5 endo-β-mannanase (GH5-7) and two putative GH2 mannan-degrading enzymes (GH2-1 and GH2-4) in Neurospora crassa. Real-time

Variable expression of leukocyte cytosolic broad-specificity beta-glucosidase activity.

G W Forsyth et al.

Clinica chimica acta; international journal of clinical chemistry, 216(1-2), 11-21 (1993-07-16)

The cytosolic beta-glucosidase activity that is found in a variety of mammalian tissues has no clearly defined function. In vitro assay conditions under which the broad-specificity beta-glucosidase hydrolyzes glucocerebroside at a significant rate have not been described. Nonetheless, it has

ABC transporter A3 facilitates lysosomal sequestration of imatinib and modulates susceptibility of chronic myeloid leukemia cell lines to this drug.

Bjoern Chapuy et al.

Haematologica, 94(11), 1528-1536 (2009-11-03)

Inhibition of BCR-ABL tyrosine kinase activity has evolved as a mainstay of therapy for patients with chronic myeloid leukemia. However, a fraction of leukemic cells persists under targeted therapy and can lead to disease progression on cessation of treatment. We

Measurement of caprine plasma beta-mannosidase with a p-nitrophenyl substrate.

K Cavanagh et al.

American journal of veterinary research, 44(4), 681-684 (1983-04-01)

Experimental conditions for measuring caprine plasma beta-D-mannosidase activity are described with p-nitrophenyl-beta-D-mannopyranoside as a substrate. The plasma enzyme was stable for 3 months at -20 C or 1 week at 4 C. The optimal pH for activity was 5.0 in

Evaluation of enzymatic and non-enzymatic softening in low salt cucumber fermentations

Maruvada R and McFeeters RF

International Journal of Food Science and Technology, 44(6), 1108-1117 (2009)

Sensitive and rapid electrochemical bioassay of glycosidase activity.

Tanin Tangkuaram et al.

The Analyst, 131(8), 889-891 (2006-10-10)

Here we present a highly sensitive, rapid and simple electrochemical assay for glycosidases based on treatment of the glycosidase with the appropriate p-nitrophenyl glycoside and anodic detection of released p-nitrophenol. The attractive characteristics of the new bioassay should facilitate advanced

Alternative substrates for use in the detection of goats heterozygous for beta-mannosidosis.

P J Healy et al.

Research in veterinary science, 33(1), 73-75 (1982-07-01)

Expression and characterization of an extremely thermostable β-glycosidase (mannosidase) from the hyperthermophilic archaeon Pyrococcus furiosus DSM3638.

Sung H Park et al.

New biotechnology, 28(6), 639-648 (2011-06-01)

Genomic analysis of the hyperthermophilic archaeon Pyrococcus furiosus revealed the presence of an open reading frame (ORF PF0356) similar to the enzymes in glycoside hydrolase family 1. This β-glycosidase, designated PFTG (P. furiosus thermostable glycosidase), was cloned and expressed in

A Ribose-Scavenging System Confers Colonization Fitness on the Human Gut Symbiont Bacteroides thetaiotaomicron in a Diet-Specific Manner.

Robert W P Glowacki et al.

Cell host & microbe, 27(1), 79-92 (2020-01-07)

Efficient nutrient acquisition in the human gut is essential for microbial persistence. Although polysaccharides have been well-studied nutrients for the gut microbiome, other resources such as nucleic acids and nucleosides are less studied. We describe several ribose-utilization systems (RUSs) that

Purification and characterization of a beta-D-mannosidase from the marine anaspidean Aplysia fasciata.

Giuseppina Andreotti et al.

Journal of biotechnology, 119(1), 26-35 (2005-07-21)

A beta-D-mannosidase was purified to homogeneity from visceral mass extract of Aplysia fasciata a mollusc belonging to the order Anaspidea. The purified enzyme is a homodimer with a subunit mass of 130 kDa. Temperature and pH optima of this enzyme

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