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关键词:'P3075'
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Erik H Christen et al.
Protein expression and purification, 66(2), 158-164 (2009-03-28)
Inducer-dependent prokaryotic transcriptional repressor proteins that originally evolved to orchestrate the transcriptome with intracellular and extracellular metabolite pools, have become universal tools in synthetic biology, drug discovery, diagnostics and functional genomics. Production of the repressor proteins is often limited due
David P Kuipers et al.
Biochimie, 95(2), 258-263 (2012-09-27)
The corneal epithelial tissue is a layer of rapidly growing cells that are highly glycolytic and express GLUT1 as the major glucose transporter. It has been shown that GLUT1 in L929 fibroblast cells and other cell lines can be acutely
Anne-Christine Schmidt et al.
Journal of mass spectrometry : JMS, 47(8), 949-961 (2012-08-18)
Phenylarsenic-substituted cysteine-containing peptides and proteins were completely differentiated from their unbound original forms by the coupling of reversed phase liquid chromatography with electrospray ionization mass spectrometry. The analysis of biomolecules possessing structure-stabilizing disulfide bridges after reduction provides new insights into
Athar Alam et al.
Free radical biology & medicine, 50(11), 1659-1668 (2011-03-17)
Plasmodium falciparum macrophage migration inhibitory factor (PfMIF) exhibits thioredoxin (Trx)-like oxidoreductase activity but the active site for this activity and its function have not been evaluated. A bioinformatics search revealed that the conserved CXXC motif, which is responsible for Trx-like
Shahnaz Shahidi-Noghabi et al.
Archives of insect biochemistry and physiology, 76(4), 211-222 (2011-01-22)
In this project, the uptake mechanisms and localization of two lectins from Sambucus nigra, further referred to as S. nigra agglutinin (SNA)-I and SNA-II, into insect midgut CF-203 cells were studied. SNA-I is a chimeric lectin belonging to the class
Manish Verma et al.
Biochimica et biophysica acta, 1827(1), 38-49 (2012-10-10)
The sustained opening of the mitochondrial permeability transition pore (PTP) is a decisive event in the onset of irreversible cell injury. The PTP is modulated by numerous exogenous and endogenous effectors, including mitochondrial membrane potential, ions and metabolites. Mitochondrial sirtuins
Jie Li et al.
Toxicology in vitro : an international journal published in association with BIBRA, 26(4), 603-612 (2012-02-11)
Cell viability assays have a variety of well known practical and technical limitations. All the available approaches have disadvantages, such as non-linearity, high background and cumbersome protocols. Several commonly used tetrazolium chemicals rely upon generation of a colored formazan product
John J H Shin et al.
Developmental cell, 52(4), 461-476 (2020-01-14)
Phosphoinositides, diacylglycerolpyrophosphate, ceramide-1-phosphate, and phosphatidic acid belong to a unique class of membrane signaling lipids that contain phosphomonoesters in their headgroups having pKa values in the physiological range. The phosphomonoester headgroup of phosphatidic acid enables this lipid to act as
Biyun Ni et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 18(1), 140-151 (2011-11-04)
Bax and Bak are regarded as key mediators for cytochrome c (Cyt c) release and apoptosis. Loss of Bax or Bak is often reported in human cancers and renders resistance of these cancerous cells to chemotherapy. Here, we investigated that
Tanya Fradin et al.
Journal of leukocyte biology, 104(5), 1023-1039 (2018-07-17)
A central event in the activation of the phagocyte NADPH oxidase involves binding of p67phox to the dehydrogenase region of Nox2. The identity of the binding site in Nox2 is unknown. By measuring binding of p67phox to synthetic Nox2 peptides
Marcela de Souza Santos et al.
Molecular pharmacology, 83(4), 793-804 (2013-01-15)
Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) is a versatile phospholipid that participates in many membrane-associated signaling processes. PI(4,5)P2 production at the plasma membrane (PM) depends on levels of its precursor, phosphatidylinositol 4-phosphate (PI4P), synthesized principally by two intracellular enzymes, PI4-kinases IIIα and IIIb;
Amir Zarebkohan et al.
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, 78, 19-30 (2015-06-30)
Blood-brain barrier inhibits most of drugs and genetic materials from reaching the brain. So, developing high efficiency carriers for gene and drug delivery to the brain, is the challenging area in pharmaceutical sciences. This investigation aimed to target DNA to
Ying Gao et al.
Cell research, 32(12), 1086-1104 (2022-10-26)
STING, an endoplasmic reticulum (ER) transmembrane protein, mediates innate immune activation upon cGAMP stimulation and is degraded through autophagy. Here, we report that activated STING could be transferred between cells to promote antitumor immunity, a process triggered by RAB22A-mediated non-canonical
Larry L Louters et al.
Biochimie, 95(4), 787-792 (2012-12-04)
Nitroxyl (HNO) has a unique, but varied, set of biological properties including beneficial effects on cardiac contractility and stimulation of glucose uptake by GLUT1. These biological effects are largely initiated by HNO's reaction with cysteine residues of key proteins. The
Kristel Kemper et al.
Life science alliance, 5(11) (2022-10-23)
CD3 bispecific antibodies (bsAbs) show great promise as anticancer therapeutics. Here, we show in-depth mechanistic studies of a CD3 bsAb in solid cancer, using DuoBody-CD3x5T4. Cross-linking T cells with tumor cells expressing the oncofetal antigen 5T4 was required to induce
Anna L Greenshields et al.
Cancer letters, 357(1), 129-140 (2014-12-03)
Piperine, an alkaloid from black pepper, is reported to have anticancer activities. In this study, we investigated the effect of piperine on the growth and motility of triple-negative breast cancer (TNBC) cells. Piperine inhibited the in vitro growth of TNBC
DA-En Cheng et al.
Oncology letters, 8(2), 620-626 (2014-07-12)
Tumor-associated dendritic cells (TADCs) are important in tumor immune surveillance, and it has been reported that the secretion of interleukin (IL)-10 by cancer cells is a major factor involved in the induction of TADCs in the tumor microenvironment. In the
Yíngyún Caì et al.
Journal of virology, 89(1), 844-856 (2014-10-31)
Simian hemorrhagic fever virus (SHFV) causes a severe and almost uniformly fatal viral hemorrhagic fever in Asian macaques but is thought to be nonpathogenic for humans. To date, the SHFV life cycle is almost completely uncharacterized on the molecular level.
M Wachtel et al.
Journal of cell science, 112 ( Pt 23), 4347-4356 (1999-11-24)
Regulation of epithelial and endothelial permeability is essential for proper function of compartmentalized organisms, and tyrosine phosphorylation plays an important role in this process. We analyzed the impact of protein tyrosine phosphatase (PTP) inhibition on the structure of endothelial junctional
Judith Müller et al.
Nature communications, 5, 5712-5712 (2014-12-17)
Increased expression of the Microphthalmia-associated transcription factor (MITF) contributes to melanoma progression and resistance to BRAF pathway inhibition. Here we show that the lack of MITF is associated with more severe resistance to a range of inhibitors, while its presence
Fouad Azizi
BioMed research international, 2020, 9692503-9692503 (2020-09-24)
Phosphoinositides play a regulatory role in clathrin-mediated endocytosis. However, their involvement in clathrin-independent endocytosis termed rapid endocytosis (RE), which is the mode of vesicle recycling during neurotransmitter release by transient fusion (known as kiss-and-run), has not been investigated. Here, we
Highly selective fluorescent probe for vicinal-dithiol-containing proteins and in situ imaging in living cells.
Chusen Huang et al.
Angewandte Chemie (International ed. in English), 50(33), 7551-7556 (2011-07-02)
Bingbing Li et al.
Plant physiology, 159(2), 671-681 (2012-04-25)
Protein tyrosine phosphatases (PTPases) have long been thought to be activated by reductants and deactivated by oxidants, owing to the presence of a crucial sulfhydryl group in their catalytic centers. In this article, we report the purification and characterization of
Matouš Glanc et al.
Current biology : CB, 31(9), 1918-1930 (2021-03-12)
Polar subcellular localization of the PIN exporters of the phytohormone auxin is a key determinant of directional, intercellular auxin transport and thus a central topic of both plant cell and developmental biology. Arabidopsis mutants lacking PID, a kinase that phosphorylates
A G Douen et al.
BioFactors (Oxford, England), 2(3), 153-161 (1990-07-01)
The actions of phenylarsine oxide (PAO) on hormone receptors and transport processes are reviewed with particular reference to the mechanism of insulin-stimulated sugar transport. It is suggested that as well as reaction with vicinal -SH groups, vicinal -SH/-OH and -SH/-CO2H
Cutaneous arsenical exposure induces distinct metabolic transcriptional alterations of kidney cells.
Kyle H Moore et al.
The Journal of pharmacology and experimental therapeutics (2023-09-13)
Arsenicals are deadly chemical warfare agents which primarily cause death through systemic capillary fluid leakage and hypovolemic shock. Arsenical exposure is also known to cause acute kidney injury, a condition that contributes to arsenical-associated death due to the necessity of
Phenylarsine oxide induces the cyclosporin A-sensitive membrane permeability transition in rat liver mitochondria
Lenartowicz E, et al.
Journal of Bioenergetics and Biomembranes, 23(4), 679-688 (1991)
Yao Xiao et al.
Nature plants, 6(5), 544-555 (2020-05-13)
The 3-phosphoinositide-dependent protein kinase 1 (PDK1) is a conserved master regulator of AGC kinases in eukaryotic organisms. pdk1 loss of function causes a lethal phenotype in animals and yeasts, but only mild phenotypic defects in Arabidopsis thaliana (Arabidopsis). The Arabidopsis genome
Sonal Prasad et al.
Communications biology, 3(1), 792-792 (2020-12-29)
The stereocilia of the inner ear sensory cells contain the actin-binding protein radixin, encoded by RDX. Radixin is important for hearing but remains functionally obscure. To determine how radixin influences hearing sensitivity, we used a custom rapid imaging technique to
Elaine E Wang et al.
Channels (Austin, Tex.), 5(6), 464-467 (2011-08-11)
Selective suppression of hyperactive sensory neurons is an attractive strategy for managing pathological pain. Blocking Na(+) channels to eliminate action potentials and desensitizing transduction channels can both reduce sensory neuron excitability. The novel synthetic vanilloid ligand cap-ET preserves agonist activation
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