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Yue-Meng Wan et al.
PloS one, 15(2), e0228889-e0228889 (2020-02-12)
Mesenchymal stem cells (MSCs) are a population of pluripotent cells that have been tested for the treatment of many inflammatory diseases. It remains unclear whether MSCs were effective in treating mice with alcoholic hepatitis (AH) and its underlying mechanism. In
Marwa Mohammed Sabry et al.
International journal of stem cells, 7(1), 33-42 (2014-06-13)
To demonstrate the bleomycin induced histological changes in the lung and the possible protective and/or therapeutic effect of stem cell therapy. Study was carried out on 36 adult male albino rats, classified into 4 groups: group I (control), group II
Naomi J Logsdon et al.
PloS one, 6(11), e28127-e28127 (2011-12-02)
Human cytomegalovirus (HCMV) expresses a viral ortholog (CMVIL-10) of human cellular interleukin-10 (cIL-10). Despite only ∼26% amino acid sequence identity, CMVIL-10 exhibits comparable immunosuppressive activity with cIL-10, attenuates HCMV antiviral immune responses, and contributes to lifelong persistence within infected hosts.
Leila Ghanbari et al.
Scientific reports, 9(1), 1023-1023 (2019-02-02)
Over the last few decades, a plethora of tools has been developed for neuroscientists to interface with the brain. Implementing these tools requires precisely removing sections of the skull to access the brain. These delicate cranial microsurgical procedures need to
Matthew Kyrish et al.
Proceedings of SPIE--the International Society for Optical Engineering, 7558, 755807-755807 (2010-08-17)
To reduce the number of invasive tissue biopsies and needle aspirations performed during cancer screenings, endo-microscopes can be used to image tissue in vivo. However, when optical fiber bundles are used to transmit the image, the resolution of such systems
Ana Carolina Vieira et al.
PloS one, 6(2), e17411-e17411 (2011-03-03)
Endogenous electric fields and currents occur naturally at wounds and are a strong signal guiding cell migration into the wound to promote healing. Many cells involved in wound healing respond to small physiological electric fields in vitro. It has long
Fabio Morandi et al.
Stem cells (Dayton, Ohio), 26(5), 1275-1287 (2008-02-23)
Human mesenchymal stem cells (MSC) are immunosuppressive and poorly immunogenic but may act as antigen-presenting cells (APC) for CD4(+) T-cell responses; here we have investigated their ability to serve as APC for in vitro CD8(+) T-cell responses. MSC pulsed with
Daniel Devine et al.
Advanced biosystems, 4(11), e2000012-e2000012 (2020-10-15)
The extracellular matrix varies considerably in mechanical properties at the microscale. It remains unclear how cells respond to these properties, in part, due to lack of tools to create precisely defined microenvironments in a discrete manner. Here, freeform stereolithography is
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Amanda Ooi et al.
Frontiers in physiology, 7, 300-300 (2016-08-04)
The human embryonic kidney 293 (HEK-293) cells are commonly used as host for the heterologous expression of membrane proteins not least because they have a high transfection efficiency and faithfully translate and process proteins. In addition, their cell size, morphology
Lubica Grausova et al.
PloS one, 6(6), e20943-e20943 (2011-06-23)
Intrinsic nanocrystalline diamond (NCD) films have been proven to be promising substrates for the adhesion, growth and osteogenic differentiation of bone-derived cells. To understand the role of various degrees of doping (semiconducting to metallic-like), the NCD films were deposited on
Bao Wang et al.
Autophagy, 12(8), 1215-1228 (2016-05-14)
As a selective degradation system, chaperone-mediated autophagy (CMA) is essential for maintaining cellular homeostasis and survival under stress conditions. Increasing evidence points to an important role for the dysfunction of CMA in the pathogenesis of Parkinson disease (PD). However, the
Distribution and Neurochemistry of the Porcine Ileocaecal Valve Projecting Sensory Neurons in the Dorsal Root Ganglia and the Influence of Lipopolysaccharide from Different Serotypes of Salmonella spp. on the Chemical Coding of DRG Neurons in the Cell Cultures
Mikolajczyk A, et al.
International Journal of Molecular Sciences, 19(9), 2551-2551 (2018)
Neda Gilannejad et al.
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 253, 110868-110868 (2020-12-15)
The influence of diurnal and nocturnal feeding on daily rhythms of gut levels of cholecystokinin (CCK) and the activity of two key pancreatic proteases, trypsin and chymotrypsin, were examined in juveniles of Senegalese sole (Solea senegalensis), a species with nocturnal
Jae-Hoon Lee et al.
TheScientificWorldJournal, 2013, 930798-930798 (2013-06-06)
A new method with a simple algorithm was developed to accurately measure Poisson's ratio of soft materials such as polyvinyl alcohol hydrogel (PVA-H) with a custom experimental apparatus consisting of a tension device, a micro X-Y stage, an optical microscope
Gaozhe Cai et al.
Micromachines, 9(12) (2018-11-30)
Immunomagnetic separation has been widely used for the separation and concentration of foodborne pathogens from complex food samples, however it can only handle a small volume of samples. In this paper, we presented a novel fluidic device for the specific
Giorgio Virnicchi et al.
Frontiers in cell and developmental biology, 8, 857-857 (2020-10-13)
Formation of the hatching mouse blastocyst marks the end of preimplantation development, whereby previous cell cleavages culminate in the formation of three distinct cell lineages (trophectoderm, primitive endoderm and epiblast). We report that dysregulated expression of Wwc2, a genetic paralog
Beatriz del Rio et al.
Clinical and vaccine immunology : CVI, 15(9), 1429-1435 (2008-07-18)
Mucosal immunization is advantageous over other routes of antigen delivery because it can induce both mucosal and systemic immune responses. Our goal was to develop a mucosal delivery vehicle based on bacteria generally regarded as safe, such as Lactobacillus spp.
M Maegele et al.
Physiological research, 64(1), 129-145 (2014-09-07)
To determine whether the exposure to long term enriched environment (EE) would result in a continuous improvement of neurological recovery and ameliorate the loss of brain tissue after traumatic brain injury (TBI) vs. standard housing (SH). Male Sprague-Dawley rats (300-350
Edit Hrubi et al.
Human cell, 31(2), 139-148 (2018-02-15)
Bone morphogenetic protein-2 (BMP-2), is a potential factor to enhance osseointegration of dental implants. However, the appropriate cellular system to investigate the osteogenic effect of BMP-2 in vitro in a standardized manner still needs to be defined. The aim of
Shoumyo Majumdar et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 104(2), 300-307 (2015-03-15)
Collagen vitrigel membranes are transparent biomaterials characterized by a densely organized, fibrillar nanostructure that show promise in the treatment of corneal injury and disease. In this study, the influence of different type I collagen sources and processing techniques, including acid-solubilized
Leonie Fingerhut et al.
Cells, 8(12) (2019-12-01)
Equine recurrent uveitis (ERU) is considered one of the most important eye diseases in horses and typically appears with relapsing inflammatory episodes without systemic effects. Various disorders have been described as an initial trigger, including infections. Independent of the initiating
Anju M Philip et al.
Developmental and comparative immunology, 45(1), 133-140 (2014-03-07)
Anadromous Arctic charr (Salvelinus alpinus) undergo voluntary winter fasting for months in the Arctic. We tested the hypothesis that extended fasting will compromise the ability of this species to evoke an immune response. Charr were either fed or fasted for
Tayo Sanders et al.
Pharmaceutics, 12(4) (2020-04-30)
The ability of viruses to introduce genetic material into cells can be usefully exploited in a variety of therapies and also vaccination. Encapsulating viruses to limit inactivation by the immune system before reaching the desired target and allowing for controlled
Clément Brunner et al.
Neuron, 108(5), 861-875 (2020-10-21)
Imaging large-scale circuit dynamics is crucial to understanding brain function, but most techniques have a limited depth of field. Here, we describe volumetric functional ultrasound imaging (vfUSI), a platform for brain-wide vfUSI of hemodynamic activity in awake head-fixed mice. We
Arkadiusz Gertych et al.
Cytometry. Part A : the journal of the International Society for Analytical Cytology, 75(7), 569-583 (2009-05-22)
Today's advanced microscopic imaging applies to the preclinical stages of drug discovery that employ high-throughput and high-content three-dimensional (3D) analysis of cells to more efficiently screen candidate compounds. Drug efficacy can be assessed by measuring response homogeneity to treatment within
Olgica Mihaljevic et al.
Mutagenesis, 33(5-6), 351-357 (2018-11-28)
As apoptosis and genome instability in children with autoimmune diseases (AIDs) are insufficiently investigated, we aimed to analyse them in peripheral blood lymphocytes (PBLs) of children and adolescents with Hashimoto's thyroiditis (HT), Graves' disease (GD) and type 1 diabetes mellitus
Deepali Rathore et al.
Current protocols in immunology, 130(1), e105-e105 (2020-09-17)
Immune cell signaling is largely regulated by protein phosphorylation. Stimulation of toll-like receptors (TLRs) by pathogen-associated ligands drives the cascade of immune response, which can be influenced by differences in phosphoprotein abundance. Therefore, the analysis of phosphorylation signatures at a
Seetharam Deepa et al.
Theriogenology, 161, 161-175 (2020-12-18)
Wnt signaling is conserved among all species and plays a significant role in various cellular processes including reproduction. The present study identified significant involvement of wnt4a, wnt5b, and wnt8a signaling in the testicular growth of common carp,Cyprinus carpio. Predominant expression
Chunyan Wang et al.
Nature communications, 7, 10319-10319 (2016-01-20)
DNA hybridization thermodynamics is critical for accurate design of oligonucleotides for biotechnology and nanotechnology applications, but parameters currently in use are inaccurately extrapolated based on limited quantitative understanding of thermal behaviours. Here, we present a method to measure the ΔG°
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