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Andrew D Pucker et al.
Investigative ophthalmology & visual science, 51(6), 2949-2954 (2010-01-22)
The purpose of this work was to develop an enzymatic method of quantification of cholesterol and cholesterol esters derived from contact lenses, both in vitro and ex vivo. Lotrafilcon B (O2 Optix; CIBA Vision, Inc., Duluth, GA) and galyfilcon A
Sang-Ah Kim et al.
Molecular cell, 78(5), 903-914 (2020-05-13)
LSD1 (lysine specific demethylase; also known as KDM1A), the first histone demethylase discovered, regulates cell-fate determination and is overexpressed in multiple cancers. LSD1 demethylates histone H3 Lys4, an epigenetic mark for active genes, but requires the CoREST repressor to act
Sina Barghahn et al.
Frontiers in plant science, 12, 682439-682439 (2021-07-06)
Plants detect conserved microbe-associated molecular patterns (MAMPs) and modified "self" molecules produced during pathogen infection [danger associated molecular patterns (DAMPs)] with plasma membrane-resident pattern recognition receptors (PRRs). PRR-mediated MAMP and/or DAMP perception activates signal transduction cascades, transcriptional reprogramming and plant
Veronika Binder et al.
Arteriosclerosis, thrombosis, and vascular biology, 33(5), 1020-1027 (2013-03-16)
Elevated levels of advanced oxidation protein products have been described in several chronic inflammatory diseases, like chronic renal insufficiency, rheumatoid arthritis, and atherosclerosis. Recent findings revealed that advanced oxidation protein products are inhibitors of the major high-density lipoprotein receptor, scavenger
Zhimin Song et al.
Biochemical pharmacology, 178, 114088-114088 (2020-06-13)
Phagocytes, especially neutrophils, can produce reactive oxygen species (ROS), through the activation of the NADPH oxidase (NOX2). Although this enzyme is crucial for host-pathogen defense, ROS production by neutrophils can be harmful in several pathologies such as cardiovascular diseases or
[NETs in pathogenesis of vasculitis].
Daigo Nakazawa et al.
Nihon Jinzo Gakkai shi, 56(2), 117-123 (2014-04-16)
Fernando Navarrete et al.
PLoS pathogens, 17(6), e1009641-e1009641 (2021-06-25)
Biotrophic plant pathogens secrete effector proteins to manipulate the host physiology. Effectors suppress defenses and induce an environment favorable to disease development. Sequence-based prediction of effector function is impeded by their rapid evolution rate. In the maize pathogen Ustilago maydis
Myeloperoxidase-mediated dysfunctional high-density lipoprotein.
Godfrey S Getz et al.
Arteriosclerosis, thrombosis, and vascular biology, 34(4), 695-696 (2014-03-26)
Z S Farhangrazi et al.
Biochemistry, 33(18), 5647-5652 (1994-05-10)
At neutral pH, compound I of Arthromyces ramosus peroxidase (ARP) was stable and was reduced to ferric ARP without apparent formation of compound II upon titration with ascorbate or hydroquinone. In the titration experiments, compound II was seen as an
Erica S Schwartz et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 33(13), 5603-5611 (2013-03-29)
Visceral afferents expressing transient receptor potential (TRP) channels TRPV1 and TRPA1 are thought to be required for neurogenic inflammation and development of inflammatory hyperalgesia. Using a mouse model of chronic pancreatitis (CP) produced by repeated episodes (twice weekly) of caerulein-induced
Diana Humer et al.
International journal of molecular sciences, 20(4) (2019-02-23)
Horseradish peroxidase (HRP) is an intensely studied enzyme with a wide range of commercial applications. Traditionally, HRP is extracted from plant; however, recombinant HRP (rHRP) production is a promising alternative. Here, non-glycosylated rHRP was produced in Escherichia coli as a
Juliette Azimzadeh et al.
The Plant cell, 20(8), 2146-2159 (2008-09-02)
Plant cells have specific microtubule structures involved in cell division and elongation. The tonneau1 (ton1) mutant of Arabidopsis thaliana displays drastic defects in morphogenesis, positioning of division planes, and cellular organization. These are primarily caused by dysfunction of the cortical
Yaiza Montes-Cebrián et al.
Sensors (Basel, Switzerland), 19(24) (2019-12-11)
Considerable efforts are made to develop Point-of-Care (POC) diagnostic tests. POC devices have the potential to match or surpass conventional systems regarding time, accuracy, and cost, and they are significantly easier to operate by or close to the patient. This
A V Razygraev
Biomeditsinskaia khimiia, 59(6), 636-643 (2014-02-12)
Recently it was shown that the presence of rat blood plasma (as well as of erythrocyte hemolysate) in the reaction mixture containing 43 mM Tris-HCl-buffer (pH 8.5), 0.29 mM EDTA, 19.2 mM sodium azide, 1 mM DL-homocysteine (Hcy), and 198
Jalal Soubhye et al.
Journal of medicinal chemistry, 56(10), 3943-3958 (2013-04-16)
Due to its production of potent antimicrobial oxidants including hypochlorous acid, human myeloperoxidase (MPO) plays a critical role in innate immunity and inflammatory diseases. Thus MPO is an attractive target in drug design. (Aminoalkyl)fluoroindole derivatives were detected to be very
G S Murzagulov et al.
Zhurnal evoliutsionnoi biokhimii i fiziologii, 49(1), 24-29 (2013-05-15)
The work deals with effect of density of population on functional activity of components pf protective system of adult individuals and nymphs of the marble cockroach. The resistance of individuals has been noted to decrease both at individual maintenance and
Matthias Karer et al.
Inflammation research : official journal of the European Histamine Research Society ... [et al.], 71(4), 497-511 (2022-03-19)
To evaluate the contribution of endogenous diamine oxidase (DAO) in the inactivation of exogenous histamine, to find a mouse strain with increased histamine sensitivity and to test the efficacy of rhDAO in a histamine challenge model. Diamine oxidase knockout (KO)
Nicole M Sayles et al.
Cell reports, 38(10), 110475-110475 (2022-03-10)
Mitochondrial cardiomyopathies are fatal diseases, with no effective treatment. Alterations of heart mitochondrial function activate the mitochondrial integrated stress response (ISRmt), a transcriptional program affecting cell metabolism, mitochondrial biogenesis, and proteostasis. In humans, mutations in CHCHD10, a mitochondrial protein with
Aitken et al.
Biotechnology progress, 14(3), 487-492 (1998-06-17)
Coprinus cinereus peroxidase (CIP) and other peroxidases are susceptible to mechanism-based inactivation during the oxidation of phenolic substrates. The turnover capacity (defined as the molar or mass concentration of substrate oxidized per unit concentration of enzyme inactivated) of CIP was
The isolation and purification of lactoperoxidase by ion exchange chromatography.
M MORRISON et al.
The Journal of biological chemistry, 228(2), 767-776 (1957-10-01)
Edwin van Bloois et al.
Applied microbiology and biotechnology, 83(4), 679-687 (2009-02-19)
Streptomyces coelicolor A3(2) alditol oxidase (AldO) is a soluble monomeric flavoprotein in which the flavin cofactor is covalently linked to the polypeptide chain. AldO displays high reactivity towards different polyols such as xylitol and sorbitol. These characteristics make AldO industrially
R Adam Shane et al.
Chemical research in toxicology, 15(10), 1324-1329 (2002-10-22)
Two supercoiled (SC), double-stranded DNAs, pBR 322 and pUC 19, have been subjected to oxidative stress using horseradish peroxidase (HRP) and HRP + tert-butyl hydroperoxide (BOOH). HRP alone causes single-strand cleavage of these SC DNAs. Strand cleavage is enhanced substantially
R Sorrentino et al.
British journal of pharmacology, 154(4), 864-871 (2008-06-10)
Lung epithelial cells express pattern recognition receptors, which react to bacteria. We have evaluated the effect of Gram-positive and Gram-negative bacteria on interleukin-8 (CXCL8) release from epithelial cells and the integrity of the epithelial barrier. Primary cultures of human airway
Bin Ye et al.
Biochemical and biophysical research communications, 434(3), 547-551 (2013-04-16)
Stress ulcers are a common complication in critically ill patients, but the underlying mechanism is little known. This study characterized the function of the p50 subunit of NF-κB in an experimental model of cold immobilization stress-induced gastric ulcers. Stress-induced gastric
Katarzyna Grychowska et al.
European journal of medicinal chemistry, 236, 114329-114329 (2022-04-10)
The incorporation of the fluorine motif is a strategy widely applied in drug design for modulating the activity, physicochemical parameters, and metabolic stability of chemical compounds. In this study, we attempted to reduce the affinity for ether-à-go-go-related gene (hERG) channel
D Saha et al.
Journal of environmental biology, 33(5), 861-866 (2013-06-06)
Tea [Camellia sinensis L. (O.) Kuntze] is an economically important plantation crop of India but is prone to attack by several fungal pathogens. Copper based fungicides are being used for decades to control fungal diseases in tea which may lead
Soni Deshwal et al.
Methods in molecular biology (Clifton, N.J.), 1782, 403-418 (2018-06-01)
Reactive oxygen species (ROS) are involved in both physiological and pathological processes. This widely accepted concept is based more on the effects of antioxidant interventions than on reliable assessments of rates and sites of intracellular ROS formation. This argument applies
Andrea Camattari et al.
Methods in molecular biology (Clifton, N.J.), 1152, 113-123 (2014-04-20)
The methylotrophic yeast Pichia pastoris is becoming one of the favorite industrial workhorses for protein expression. Due to the widespread use of integration vectors, which generates significant clonal variability, screening methods allowing assaying hundreds of individual clones are of particular
Xinye Zhang et al.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 130(6), 1155-1168 (2017-03-16)
Loci associated with variation in maize responses to two microbe-associated molecular patterns (MAMPs) were identified. MAMP responses were correlated. No relationship between MAMP responses and quantitative disease resistance was identified. Microbe-associated molecular patterns (MAMPs) are highly conserved molecules commonly found
Erica Kintz et al.
Journal of bacteriology, 190(8), 2709-2716 (2007-12-11)
The Wzz proteins are important for determining the length of the O-antigen side chain attached to lipopolysaccharide (LPS). Several bacteria, including Pseudomonas aeruginosa strain PAO1 (serogroup O5), produce two such proteins responsible for the preference of two different chain lengths
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