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Olivera Magdenoska et al.
Analytical biochemistry, 487, 17-26 (2015-07-05)
For quantification of intracellular metabolites, mass spectrometry combined with liquid chromatography, capillary electrophoresis, or gas chromatography is currently the method of choice, especially when combined with stable isotope-labeled internal standards (SIL-ISs). Due to the difficulties in finding a biological matrix
Nicholas Light et al.
Epigenetics, 9(9), 1238-1251 (2014-07-24)
Allele-specific (AS) assessment of chromatin has the potential to elucidate specific cis-regulatory mechanisms, which are predicted to underlie the majority of the known genetic associations to complex disease. However, development of chromatin landscapes at allelic resolution has been challenging since
G W Mainwood et al.
Advances in experimental medicine and biology, 248, 611-619 (1989-01-01)
In this study we measured changes in intracellular ATP and pH together with lactate production in isolated ischemic human atrial tissue. The measurements were made using 31P and 1H NMR. ATP preservation is improved as temperature is reduced from 20
Serap Erkek et al.
Cancer cell, 35(1), 95-110 (2019-01-01)
Biallelic inactivation of SMARCB1, encoding a member of the SWI/SNF chromatin remodeling complex, is the hallmark genetic aberration of atypical teratoid rhabdoid tumors (ATRT). Here, we report how loss of SMARCB1 affects the epigenome in these tumors. Using chromatin immunoprecipitation
Kyle M Burns et al.
Protein science : a publication of the Protein Society, 24(8), 1313-1324 (2015-05-27)
Water-mediated hydrogen exchange (HX) processes involving the protein main chain are sensitive to structural dynamics and molecular interactions. Measuring deuterium uptake in amide bonds provides information on conformational states, structural transitions and binding events. Increasingly, deuterium levels are measured by
Ivo A Hendriks et al.
Nature communications, 6, 7289-7289 (2015-06-16)
SUMOylation is a reversible post-translational modification (PTM) regulating all nuclear processes. Identification of SUMOylation sites by mass spectrometry (MS) has been hampered by bulky tryptic fragments, which thus far necessitated the use of mutated SUMO. Here we present a SUMO-specific
Stéphanie Sylvain-Prévost et al.
Journal of immunology (Baltimore, Md. : 1950), 195(11), 5393-5403 (2015-10-23)
The MAP3 kinase, TAK1, is known to act upstream of IKK and MAPK cascades in several cell types, and is typically activated in response to cytokines (e.g., TNF, IL-1) and TLR ligands. In this article, we report that in human
Takanobu Kan et al.
Allergology international : official journal of the Japanese Society of Allergology, 64(3), 266-271 (2015-06-29)
We previously identified MGL_1304 secreted by Malassezia globosa as a sweat antigen for patients with atopic dermatitis (AD) and cholinergic urticaria (ChU). However, purifying native MGL_1304 from human sweat or culture supernatant of M. globosa (sup-MGL_1304) is costly and time-consuming.
Isabel C O Morais et al.
PloS one, 10(7), e0132569-e0132569 (2015-07-21)
Acute renal failure is a common complication caused by Bothrops viper envenomation. In this study, the nefrotoxicity of a main component of B. leucurus venom called L-aminoacid oxidase (LAAO-Bl) was evaluated by using tubular epithelial cell lines MDCK and HK-2
Barbara N Borsos et al.
Developmental biology, 404(1), 80-87 (2015-05-12)
The molting during Drosophila development is tightly regulated by the ecdysone hormone. Several steps of the ecdysone biosynthesis have been already identified but the regulation of the entire process has not been clarified yet. We have previously reported that dATAC
Joanna C Young et al.
Nature communications, 5, 5887-5887 (2014-12-20)
The hallmark of enteropathogenic Escherichia coli (EPEC) infection is the formation of actin-rich pedestal-like structures, which are generated following phosphorylation of the bacterial effector Tir by cellular Src and Abl family tyrosine kinases. This leads to recruitment of the Nck-WIP-N-WASP
Huan Wang et al.
The British journal of nutrition, 114(4), 519-532 (2015-07-23)
The aim of the present study was to investigate the mechanistic basis of protein deficiency during pregnancy in mother that is transduced to offspring. To this end, timed-pregnant Sprague-Dawley rats were fed either a control (20 % of energy from
Katie M Lowther et al.
Biology of reproduction, 93(2), 43-43 (2015-07-03)
During oocyte maturation, fertilization, and early embryo development until zygotic genome activation (ZGA), transcription is suppressed, and gene expression is dependent upon the timely activation of stored mRNAs. Embryonic poly(A)-binding protein (EPAB) is the predominant poly(A)-binding protein in Xenopus, mouse
Paul D Dinkel et al.
Biochemistry, 54(30), 4731-4740 (2015-07-16)
Tau fibrils are the main proteinacious components of neurofibrillary lesions in Alzheimer disease. Although RNA molecules are sequestered into these lesions, their relationship to Tau fibrils is only poorly understood. Such understanding, however, is important, as short fibrils can transfer
Aurélien Chollet et al.
Journal of structural biology, 190(3), 328-337 (2015-04-22)
InhA is an enoyl-ACP reductase of Mycobacterium tuberculosis implicated in the biosynthesis of mycolic acids, essential constituents of the mycobacterial cell wall. To date, this enzyme is considered as a promising target for the discovery of novel antitubercular drugs. In
Takayuki Sekimoto et al.
Molecular and cellular biology, 35(4), 699-715 (2014-12-10)
DNA rereplication is a major form of aberrant replication that causes genomic instabilities, such as gene amplification. However, little is known about which DNA polymerases are involved in the process. Here, we report that low-fidelity Y-family polymerases (Y-Pols), Pol η
Ranran Wang et al.
Cancer research, 74(12), 3332-3343 (2014-04-17)
BRD4 is implicated in the pathogenesis of a number of different cancers. It is also the target of translocation t(15;19) that accounts for the highly aggressive NUT midline carcinoma (NMC). We discovered that t(15;19) NMC cells display the ability to
S Pandolfi et al.
Cell death and differentiation, 22(12), 2006-2019 (2015-05-30)
HEDGEHOG (HH) signaling is a key regulator of tissue development and its aberrant activation is involved in several cancer types, including melanoma. We and others have shown a reciprocal cross talk between HH signaling and p53, whose function is often
Véronique Martel-Frachet et al.
Oncotarget, 6(16), 14669-14686 (2015-06-04)
We previously identified 1-(2,4-dimethoxyphenyl)-3-(1-methylindolyl) propenone (IPP51), a new chalcone derivative that is capable of inducing prometaphase arrest and subsequent apoptosis of bladder cancer cells. Here, we demonstrate that IPP51 selectively inhibits proliferation of tumor-derived cells versus normal non-tumor cells. IPP51
Yeast Coq9 controls deamination of coenzyme Q intermediates that derive from para-aminobenzoic acid.
Cuiwen H He et al.
Biochimica et biophysica acta, 1851(9), 1227-1239 (2015-05-27)
Coq9 is a polypeptide subunit in a mitochondrial multi-subunit complex, termed the CoQ-synthome, required for biosynthesis of coenzyme Q (ubiquinone or Q). Deletion of COQ9 results in dissociation of the CoQ-synthome, but over-expression of Coq8 putative kinase stabilizes the CoQ-synthome
B W Nielsen et al.
Agents and actions, 35(3-4), 170-178 (1992-03-01)
Increased osmotic pressure has been reported to cause non-cytotoxic histamine release (HR) from human basophils, as well as a potentiation of HR induced by anti-IgE. In this study, the effects of hyperosmolar Na-K-acetate (300-600 mOsm/kg H2O) on HR was studied
Sergio A Mojica et al.
Molecular biology of the cell, 26(10), 1918-1934 (2015-03-20)
SINC, a new type III secreted protein of the avian and human pathogen Chlamydia psittaci, uniquely targets the nuclear envelope of C. psittaci-infected cells and uninfected neighboring cells. Digitonin-permeabilization studies of SINC-GFP-transfected HeLa cells indicate that SINC targets the inner
Axel Freischmidt et al.
BMC neuroscience, 16, 77-77 (2015-11-18)
The recent identification of several mutations in PFN1, a protein involved in actin dynamics, strengthens the hypothesis that pathology of amyotrophic lateral sclerosis is linked to cytoskeletal defects. Impaired actin binding is a common denominator of several PFN1 mutations associated
J Mano et al.
Biochimica et biophysica acta, 1480(1-2), 374-376 (2000-09-27)
Arabidopsis thaliana P1 protein was crystallized using the hanging drop vapor-diffusion method in 0.1 M piperazine-1, 4-bis(2-ethanesulfonic acid) buffer, containing 14% polyethylene glycol 6000 and 0.2 M magnesium acetate at pH 6.5 and 20 degrees C. The crystals are orthorhombic
Qi Miao et al.
Nature communications, 5, 4088-4088 (2014-06-10)
Cell migration is an integral part of re-epithelialization during skin wound healing, a complex process involving molecular controls that are still largely unknown. Here we identify a novel role for Tcf3, an essential transcription factor regulating embryonic and adult skin
A N Koppes et al.
Journal of neural engineering, 11(4), 046002-046002 (2014-06-04)
Both electrical stimuli (endogenous and exogenous) and topographical cues are instructive to axonal extension. This report, for the first time, investigated the relative dominance of directional topographical guidance cues and directional electrical cues to enhance and/or direct primary neurite extension.
Kaisorn L Chaichana et al.
Journal of neuroscience methods, 180(1), 116-125 (2009-05-12)
The discoveries of neural (NSCs) and brain tumor stem cells (BTSCs) in the adult human brain and in brain tumors, respectively, have led to a new era in neuroscience research. These cells represent novel approaches to studying normal phenomena such
V D Prokopieva et al.
Alcohol and alcoholism (Oxford, Oxfordshire), 35(1), 44-48 (2000-02-24)
The effects of carnosine and related compounds on erythrocytes from alcoholics were studied. In their presence, erythrocytes showed an increased ability to resist haemolysis and showed a more normal morphology, with carnosine and N-acetyl-carnosine being the most effective compounds. These
R L Schultz et al.
Journal of neurocytology, 15(4), 461-469 (1986-08-01)
When used for vascular perfusion of brain, 0.1 M PIPES-buffered 3% glutaraldehyde resulted in the formation of expanded, vesicle-filled cell processes limited by multiple membrane layers. These structures, termed multivesicular myelin figures and interpreted as artefacts, were most common in
E R Swenson et al.
The American journal of physiology, 250(2 Pt 2), F288-F293 (1986-02-01)
Maximal rates of renal hydrogen ion secretion and bicarbonate reabsorption in the dogfish were stimulated by intravascular infusion of acidic and basic buffers: bicarbonate, phosphate, phenol red, dimethadione (DMO), imidazole, and piperazine-N,N'-bis(2 ethanesulfonic acid) (PIPES). There was no difference in
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