P9416

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Secreted osteopontin is highly polymerized in human airways and fragmented in asthmatic airway secretions.

Mehrdad Arjomandi et al.

PloS one, 6(10), e25678-e25678 (2011-10-28)

Osteopontin (OPN) is a member of the small integrin-binding ligand N-linked glycoprotein (SIBLING) family and a cytokine with diverse biologic roles. OPN undergoes extensive post-translational modifications, including polymerization and proteolytic fragmentation, which alters its biologic activity. Recent studies suggest that

Optimization of Tetrahydroindazoles as Inhibitors of Human Dihydroorotate Dehydrogenase and Evaluation of Their Activity and In Vitro Metabolic Stability.

Gergana Popova et al.

Journal of medicinal chemistry, 63(8), 3915-3934 (2020-03-28)

Human dihydroorotate dehydrogenase (DHODH), an enzyme in the de novo pyrimidine synthesis pathway, is a target for the treatment of rheumatoid arthritis and multiple sclerosis and is re-emerging as an attractive target for cancer therapy. Here we describe the optimization

Preparation of Cerebellum Granule Neurons from Mouse or Rat Pups and Evaluation of Clostridial Neurotoxin Activity and Their Inhibitors by Western Blot and Immunohistochemistry.

Domenico Azarnia Tehran et al.

Bio-protocol, 8(13), e2918-e2918 (2018-07-05)

Cerebellar Granule Neurons (CGN) from post-natal rodents have been widely used as a model to study neuronal development, physiology and pathology. CGN cultured in vitro maintain the same features displayed in vivo by mature cerebellar granule cells, including the development

Measurement of RNA-induced PKR Activation in vitro.

Kobe C Yuen

Bio-protocol, 7(6), e2178-e2178 (2017-03-20)

Protein kinase R (PKR) is one of the key RNA-activated sensors for innate immunity. PKR is activated by pathogenic or aberrant RNAs such as short double-stranded RNAs or those with imperfect secondary structures, as well as a reduction in the

Pericytes in the myovascular niche promote post-natal myofiber growth and satellite cell quiescence.

Enis Kostallari et al.

Development (Cambridge, England), 142(7), 1242-1253 (2015-03-07)

The satellite cells, which serve as adult muscle stem cells, are both located beneath myofiber basement membranes and closely associated with capillary endothelial cells. We observed that 90% of capillaries were associated with pericytes in adult mouse and human muscle.

A liquid biopsy for detecting circulating mesothelial precursor cells: A new biomarker for diagnosis and prognosis in mesothelioma.

Bill T V Duong et al.

EBioMedicine, 61, 103031-103031 (2020-10-13)

Malignant pleural mesothelioma (MPM) is an aggressive cancer related to asbestos exposure. Early diagnosis is challenging due to generic symptoms and a lack of biomarkers. We previously demonstrated that mesothelial precursor cells (MPC) characterized by mesothelin (MSLN)+CD90+CD34+ could be implicated

Molecular alterations of the TLR4-signaling cascade in canine epilepsy.

Eva-Lotta von Rüden et al.

BMC veterinary research, 16(1), 18-18 (2020-01-22)

Cumulating evidence from rodent models points to a pathophysiological role of inflammatory signaling in the epileptic brain with Toll-like receptor-4 signaling acting as one key factor. However, there is an apparent lack of information about expression alterations affecting this pathway

A Proliferation-Inducing Ligand Regulation in Polymorphonuclear Neutrophils by Panax ginseng.

Wioletta Ratajczak-Wrona et al.

Archivum immunologiae et therapiae experimentalis, 68(6), 32-32 (2020-10-31)

A proliferation-inducing ligand (APRIL) is a member of the tumor necrosis factor superfamily that was first identified as a factor favoring tumorigenesis. APRIL is important fitness and survival factors for B cells and plasma cells in the periphery. Considering this

Retrospective cell lineage reconstruction in humans by using short tandem repeats.

Liming Tao et al.

Cell reports methods, 1(3), None-None (2021-08-04)

Cell lineage analysis aims to uncover the developmental history of an organism back to its cell of origin. Recently, novel in vivo methods utilizing genome editing enabled important insights into the cell lineages of animals. In contrast, human cell lineage remains

Epidemiological survey of serum titers from adults against various Gram-negative bacterial V-antigens.

Mao Kinoshita et al.

PloS one, 15(3), e0220924-e0220924 (2020-03-11)

The V-antigen, a virulence-associated protein, was first identified in Yersinia pestis more than half a century ago. Since then, other V-antigen homologs and orthologs have been discovered and are now considered as critical molecules for the toxic effects mediated by

Targeting the TXNIP-NLRP3 interaction with PSSM1443 to suppress inflammation in sepsis-induced myocardial dysfunction.

Linhua Wang et al.

Journal of cellular physiology, 236(6), 4625-4639 (2021-01-17)

Sepsis-induced myocardial dysfunction (SIMD), a deadly symptom in sepsis patients, is mainly caused by cardiovascular inflammation. However, it remains unclear how systemic inflammation triggers and aggravates cardiovascular inflammation in the pathogenesis of SIMD. This study found that proinflammatory cytokines and

Direct kinetic fingerprinting and digital counting of single protein molecules.

Tanmay Chatterjee et al.

Proceedings of the National Academy of Sciences of the United States of America, 117(37), 22815-22822 (2020-09-02)

The sensitive and accurate quantification of protein biomarkers plays important roles in clinical diagnostics and biomedical research. Sandwich ELISA and its variants accomplish the capture and detection of a target protein via two antibodies that tightly bind at least two

The Genomic History of the Bronze Age Southern Levant.

Lily Agranat-Tamir et al.

Cell, 181(5), 1146-1157 (2020-05-30)

We report genome-wide DNA data for 73 individuals from five archaeological sites across the Bronze and Iron Ages Southern Levant. These individuals, who share the "Canaanite" material culture, can be modeled as descending from two sources: (1) earlier local Neolithic

Protein Synthesis in the Developing Neocortex at Near-Atomic Resolution Reveals Ebp1-Mediated Neuronal Proteostasis at the 60S Tunnel Exit.

Matthew L Kraushar et al.

Molecular cell, 81(2), 304-322 (2020-12-29)

Protein synthesis must be finely tuned in the developing nervous system as the final essential step of gene expression. This study investigates the architecture of ribosomes from the neocortex during neurogenesis, revealing Ebp1 as a high-occupancy 60S peptide tunnel exit

Multiplexed Sequential DNA FISH in Caenorhabditis elegans Embryos.

Ahilya N Sawh et al.

STAR protocols, 1(3), 100107-100107 (2020-12-31)

This protocol describes a high-throughput and multiplexed DNA fluorescence in situ hybridization method to trace chromosome conformation in Caenorhabditis elegans embryos. This approach generates single-cell and single-chromosome localization data that can be used to determine chromosome conformation and assess the

Point mutation in the mouse P2X7 receptor affects intercellular calcium waves in astrocytes.

Sylvia O Suadicani et al.

ASN neuro, 1(1), doi:10-doi:10 (2009-07-03)

Purinergic P2 receptors and gap junctions are two groups of proteins involved in the transmission of ICWs (intercellular calcium waves) between astrocytes. The extent to which ICWs spread among these glial cells depends on the amount of ATP released, which

A versatile microfluidic device for multiple ex vivo/in vitro tissue assays unrestrained from tissue topography.

Jose M de Hoyos-Vega et al.

Microsystems & nanoengineering, 6, 40-40 (2020-06-29)

Precision-cut tissue slices are an important in vitro system to study organ function because they preserve most of the native cellular microenvironments of organs, including complex intercellular connections. However, during sample manipulation or slicing, some of the natural surface topology

Feature-rich covalent stains for super-resolution and cleared tissue fluorescence microscopy.

Chenyi Mao et al.

Science advances, 6(22), eaba4542-eaba4542 (2020-06-11)

Fluorescence microscopy is a workhorse tool in biomedical imaging but often poses substantial challenges to practitioners in achieving bright or uniform labeling. In addition, while antibodies are effective specific labels, their reproducibility is often inconsistent, and they are difficult to

Dynamics of myogenic differentiation using a novel Myogenin knock-in reporter mouse.

Maria Benavente-Diaz et al.

Skeletal muscle, 11(1), 5-5 (2021-02-20)

Myogenin is a transcription factor that is expressed during terminal myoblast differentiation in embryonic development and adult muscle regeneration. Investigation of this cell state transition has been hampered by the lack of a sensitive reporter to dynamically track cells during

Gla-rich protein is a novel vitamin K-dependent protein present in serum that accumulates at sites of pathological calcifications.

Carla S B Viegas et al.

The American journal of pathology, 175(6), 2288-2298 (2009-11-07)

Mineralization of soft tissues is an abnormal process that occurs in any body tissue and can greatly increase morbidity and mortality. Vitamin K-dependent (VKD) proteins play a crucial role in these processes; matrix Gla protein is considered one of the

A Short Tandem Repeat-Enriched RNA Assembles a Nuclear Compartment to Control Alternative Splicing and Promote Cell Survival.

Karen Yap et al.

Molecular cell, 72(3), 525-540 (2018-10-16)

Functions of many long noncoding RNAs (lncRNAs) depend on their ability to interact with multiple copies of specific RNA-binding proteins (RBPs). Here, we devised a workflow combining bioinformatics and experimental validation steps to systematically identify RNAs capable of multivalent RBP

Single-cell genome sequencing at ultra-high-throughput with microfluidic droplet barcoding.

Freeman Lan et al.

Nature biotechnology, 35(7), 640-646 (2017-05-30)

The application of single-cell genome sequencing to large cell populations has been hindered by technical challenges in isolating single cells during genome preparation. Here we present single-cell genomic sequencing (SiC-seq), which uses droplet microfluidics to isolate, fragment, and barcode the

Hypoxia compensates cell cycle arrest with progenitor differentiation during angiogenesis.

Bárbara Acosta-Iborra et al.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(5), 6654-6674 (2020-03-31)

Angiogenesis, the main mechanism that allows vascular expansion for tissue regeneration or disease progression, is often triggered by an imbalance between oxygen consumption and demand. Here, by analyzing changes in the transcriptomic profile of endothelial cells (ECs) under hypoxia we

Tumor spheroids under perfusion within a 3D microfluidic platform reveal critical roles of cell-cell adhesion in tumor invasion.

Yu Ling Huang et al.

Scientific reports, 10(1), 9648-9648 (2020-06-17)

Tumor invasion within the interstitial space is critically regulated by the force balance between cell-extracellular matrix (ECM) and cell-cell interactions. Interstitial flows (IFs) are present in both healthy and diseased tissues. However, the roles of IFs in modulating cell force

Telomere analysis by fluorescence in situ hybridization and flow cytometry.

Magnus H, et al.

Nucleic Acids Research, 26(16), 3651-3656 (1998)

Modification of AlphaLISA Excitation Wavelength Leads to Improved Assay Sensitivity for Photosynthetic Tissue Samples.

Aaron J Walck et al.

SLAS technology, 24(4), 429-436 (2019-01-11)

For the purposes of high-throughput immunoassay screening, PerkinElmer's AlphaLISA technology offers many benefits over traditional enzyme-linked immunosorbant assay (ELISA). However, its 680 nm excitation wavelength coincides with a wavelength of peak photosynthetic pigment absorbance, hindering the technology's utility within the

Detection and localization of Solitalea-like and Cardinium bacteria in three Acarus siro populations (Astigmata: Acaridae).

Jan Hubert et al.

Experimental & applied acarology, 70(3), 309-327 (2016-08-10)

Bacteria associated with mites influence their fitness, nutrition and reproduction. Previously, we found Solitalea-like (Sphingobacteriales) and Candidatus Cardinium (Cytophagales) bacteria in the stored product mite Acarus siro L. by cloning and using pyrosequencing. In this study, taxon-specific primers targeting 16S

Mesenchymal Cells Support the Oncogenicity and Therapeutic Response of the Hedgehog Pathway in Triple-Negative Breast Cancer.

Ana M Reyes-Ramos et al.

Cancers, 11(10) (2019-10-30)

The paracrine interaction between tumor cells and adjacent stroma has been associated with the oncogenic activity of the Hedgehog (Hh) pathway in triple-negative breast tumors. The present study developed a model of paracrine Hh signaling and examined the impact of

Local and Systemic Concentrations of Pattern Recognition Receptors of the Lectin Pathway of Complement in a Cohort of Patients With Interstitial Lung Diseases.

Severin Vogt et al.

Frontiers in immunology, 11, 562564-562564 (2020-10-27)

The role of the lectin pathway of complement in the pathogenesis of interstitial lung diseases (ILDs) is largely unknown. Pattern recognition receptors (PRR) of the lectin pathway are involved in the clearance of apoptotic cells either via activation of the

FLEP-seq: simultaneous detection of RNA polymerase II position, splicing status, polyadenylation site and poly(A) tail length at genome-wide scale by single-molecule nascent RNA sequencing.

Yanping Long et al.

Nature protocols, 16(9), 4355-4381 (2021-08-01)

Elongation, splicing and polyadenylation are fundamental steps of transcription, and studying their coordination requires simultaneous monitoring of these dynamic processes on one transcript. We recently developed a full-length nascent RNA sequencing method in the model plant Arabidopsis that simultaneously detects

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