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Michael G Mason et al.
Nature protocols, 15(11), 3663-3677 (2020-10-03)
The complexity of current nucleic acid isolation methods limits their use outside of the modern laboratory environment. Here, we describe a fast and affordable method to purify nucleic acids from animal, plant, viral and microbial samples using a cellulose-based dipstick.
Sonia Brockway et al.
Nature communications, 11(1), 5722-5722 (2020-11-14)
Chemical-genetic interaction profiling in model organisms has proven powerful in providing insights into compound mechanism of action and gene function. However, identifying chemical-genetic interactions in mammalian systems has been limited to low-throughput or computational methods. Here, we develop Quantitative and
Diana C Cox et al.
Human molecular genetics, 29(10), 1729-1744 (2020-05-16)
mRNA processing is highly regulated during development through changes in RNA-binding protein (RBP) activities. CUG-BP, Elav-like family member 1 (CELF1, also called CUGBP1) is an RBP, the expression of which decreases in skeletal muscle soon after birth. CELF1 regulates multiple
Peng Wei et al.
Molecular and cellular neurosciences, 41(2), 258-273 (2009-04-07)
Cbln1, a glycoprotein secreted from granule cells and GluRdelta2 in the postsynaptic densities of Purkinje cells are components of an incompletely understood pathway essential for integrity and plasticity of parallel fiber-Purkinje cell synapses. We show that Cbln1 undergoes anterograde transport
Julian Nüchel et al.
Autophagy, 14(3), 465-486 (2018-01-04)
TGFB1 (transforming growth factor beta 1) is a potent cytokine playing a driving role in development, fibrosis and cancer. It is synthesized as prodomain-growth factor complex that requires tethering to LTBP (latent transforming growth factor beta binding protein) for efficient
Ye-Wheen Lim et al.
Current biology : CB, 27(13), 1968-1981 (2017-06-27)
The embryonic notochord is a flexible structure present during development that serves as scaffold for formation of the vertebrate spine. This rod-like organ is thought to have evolved in non-vertebrate chordates to facilitate locomotion by providing a rigid but flexible
Cyrine Ben Dhaou et al.
Angiogenesis (2021-09-16)
Chemerin is a multifunctional protein initially characterized in our laboratory as a chemoattractant factor for leukocyte populations. Its main functional receptor is CMKLR1. We identified previously chemerin as an anti-tumoral factor inhibiting the vascularization of tumor grafts. We show here
Sofia Moreira et al.
Cell reports, 26(2), 293-301 (2019-01-10)
Apical-basal polarity is a common trait that underlies epithelial function. Although the asymmetric distribution of cortical polarity proteins works in a functioning equilibrium, it also retains plasticity to accommodate cell division, during which the basolateral determinant Lgl is released from
Ludovico Cantuti-Castelvetri et al.
Science (New York, N.Y.), 370(6518), 856-860 (2020-10-22)
The causative agent of coronavirus disease 2019 (COVID-19) is the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). For many viruses, tissue tropism is determined by the availability of virus receptors and entry cofactors on the surface of host cells. In
Alina Bisag et al.
Cancers, 12(2) (2020-02-23)
Epithelial Ovarian Cancer (EOC) is one of the leading causes of cancer-related deaths among women and is characterized by the diffusion of nodules or plaques from the ovary to the peritoneal surfaces. Conventional therapeutic options cannot eradicate the disease and
Devin Harrison et al.
STAR protocols, 2(3), 100807-100807 (2021-09-17)
Heterogeneous metabolism supports critical single-cell functions. Here, we describe deep-learning-enabled image analyses of a genetically encoded lactate-sensing probe which can accurately quantify metabolite levels and glycolytic rates at the single-cell level. Multiple strategies and test data have been included to
Wojciech Michno et al.
Journal of neurochemistry, 152(5), 602-616 (2019-10-13)
One of the major hallmarks of Alzheimer's disease (AD) pathology is the formation of extracellular amyloid β (Aβ) plaques. While Aβ has been suggested to be critical in inducing and, potentially, driving the disease, the molecular basis of AD pathogenesis
Sara Faccidomo et al.
Psychopharmacology, 204(1), 135-147 (2009-01-07)
Extracellular signal-regulated protein kinase (ERK(1/2)) is a member of the mitogen-activated protein kinase (MAPK) signaling pathway and a key molecular target for ethanol (EtOH) and other drugs of abuse. The aim of the study was to assess the role of
Zhao Li et al.
Clinical chemistry, 66(2), 324-332 (2020-02-11)
Rapid identification of fentanyl at the point-of-care is critical. Urine fentanyl concentrations in overdose cases start at single-digit nanograms per milliliter. No fentanyl point-of-care assay with a cutoff at single-digit nanograms per milliliter is available. A competitive lateral flow assay
Binghao Li et al.
Cancer research, 78(14), 3938-3953 (2018-05-08)
The mechanism by which osteosarcomas metastasize is elusive, and challenges remain regarding its treatment with modalities including immunotherapy. CXCL12 is deeply involved in the process of tumor metastasis and T-cell homing, which is driven by a chemokine gradient, but healthy
Jan Krivanek et al.
Nature communications, 11(1), 4816-4816 (2020-09-25)
Understanding cell types and mechanisms of dental growth is essential for reconstruction and engineering of teeth. Therefore, we investigated cellular composition of growing and non-growing mouse and human teeth. As a result, we report an unappreciated cellular complexity of the
Giovanni H Diaz et al.
STAR protocols, 2(3), 100711-100711 (2021-08-18)
Ossification and the delicateness of the cochlear duct make histologic assessments of the mature cochlea a challenging endeavor. Treatments to soften the bone facilitate sectioning and dissection of the cochlear duct but limit in situ mRNA detection in such specimens.
Anna Jędrusek-Golińska et al.
Foods (Basel, Switzerland), 8(12) (2019-12-11)
The special properties of soy preparations make them common additives for food production and can be dangerous for sensitive individuals. Our aim was to check consumers' awareness of potential risks of soy preparations added to numerous food products, depending on
Dionis Minev et al.
Nature communications, 12(1), 1741-1741 (2021-03-21)
Natural biomolecular assemblies such as actin filaments or microtubules can exhibit all-or-nothing polymerization in a kinetically controlled fashion. The kinetic barrier to spontaneous nucleation arises in part from positive cooperativity deriving from joint-neighbor capture, where stable capture of incoming monomers
Julie Bejoy et al.
STAR protocols, 2(4), 100898-100898 (2021-11-09)
Several kidney diseases including congenital nephrotic syndrome, Alport syndrome, and diabetic nephropathy are linked to podocyte dysfunction. Human podocytopathies may be modeled in either primary or immortalized podocyte cell lines. Human induced pluripotent stem cell (hiPSC)-derived podocytes are a source
Ping Wang et al.
Current protocols, 1(8), e174-e174 (2021-08-06)
Chromatin Interaction Analysis Using Paired-End Tag Sequencing (ChIA-PET) is an established method to map protein-mediated chromatin interactions. A limitation, however, is that it requires a hundred million cells per experiment, which hampers its broad application in biomedical research, particularly in
Joseph G Dubouzet et al.
Plant biotechnology journal, 9(4), 466-485 (2010-10-20)
Approximately 20,000 of the rice-FOX Arabidopsis transgenic lines, which overexpress 13,000 rice full-length cDNAs at random in Arabidopsis, were screened for bacterial disease resistance by dip inoculation with Pseudomonas syringae pv. tomato DC3000 (Pst DC3000). The identities of the overexpressed
Rajesh K Chaurasia et al.
STAR protocols, 2(4), 100834-100834 (2021-09-28)
Current protocols for storage of white blood cells (WBCs) rely on constant refrigeration. The protocol described below explains the preparation of a fixative combination saline (FCS) formulation, which allows fixation of human WBCs and lysis of red blood cells and
The Chromatin Accessibility Landscape of Adult Rat.
Yue Yuan et al.
Frontiers in genetics, 12, 651604-651604 (2021-06-11)
Shekar Srinivas et al.
Immunology, 125(4), 492-502 (2008-06-13)
Interferon (IFN)-lambda1 [interleukin (IL)-29] is a member of the interferon lambda family (also known as type III interferons), whose members are distantly related to both the type I interferons and members of the IL-10 family. While IFN-lambda1 has significant antiviral
Jonathan S Duke-Cohan et al.
Vaccine, 27(8), 1154-1165 (2009-01-17)
The renewed interest in strategies to combat infectious agents with epidemic potential has led to a re-examination of vaccination protocols against smallpox. To help define which antigens elicit a human antibody response, we have targeted proteins known or predicted to
Renee M McFee et al.
Biology of reproduction, 81(5), 966-977 (2009-07-17)
We hypothesized that vascular endothelial growth factor A (VEGFA) angiogenic isoforms and their receptors, FLT1 and KDR, regulate follicular progression in the perinatal rat ovary. Each VEGFA angiogenic isoform has unique functions (based on its exons) that affect diffusibility, cell
William L Nowatzke et al.
Journal of agricultural and food chemistry, 67(1), 484-498 (2018-11-30)
An xMAP Food Allergen Detection Assay (xMAP FADA) was developed to meet analytical needs when responding to complaints by individuals with multiple food allergies and to address potential ambiguities associated with cross-reactive proteins. A single-laboratory validation (SLV) was conducted to
Carl D Walkey et al.
ACS nano, 8(3), 2439-2455 (2014-02-13)
Using quantitative models to predict the biological interactions of nanoparticles will accelerate the translation of nanotechnology. Here, we characterized the serum protein corona 'fingerprint' formed around a library of 105 surface-modified gold nanoparticles. Applying a bioinformatics-inspired approach, we developed a
Soham Gupta et al.
Frontiers in immunology, 11, 437-437 (2020-04-01)
The hijacking of cellular function through expression of proteins that interfere with the activity of cellular enzymes and regulatory complexes is a common strategy used by viruses to remodel the cell environment in favor of their own replication and spread.
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