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pcr thermocycler
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关键词:'pcr thermocycler'
显示 1-30 共 32 条结果 关于 "pcr thermocycler" 范围 论文
I Schneegass et al.
Lab on a chip, 1(1), 42-49 (2004-04-22)
Flow-through chip thermocyclers can be used in miniaturized rapid polymerase chain reaction (PCR) despite their high surface to volume ratio of samples. We demonstrated that a thermocycler made of silicon and glass chips and containing thin film transducers for heating
Momčilo Gavrilov et al.
Nature communications, 13(1), 6312-6312 (2022-10-24)
Polymerase Chain Reaction (PCR) is an essential method in molecular diagnostics and life sciences. PCR requires thermal cycling for heating the DNA for strand separation and cooling it for replication. The process uses a specialized hardware and exposes biomolecules to
Mark Keller et al.
PloS one, 10(7), e0131845-e0131845 (2015-07-07)
Nested PCR remains a labor-intensive and error-prone biomolecular analysis. Laboratory workflow automation by precise control of minute liquid volumes in centrifugal microfluidic Lab-on-a-Chip systems holds great potential for such applications. However, the majority of these systems require costly custom-made processing
Charalampos Tzivelekis et al.
PloS one, 15(10), e0240237-e0240237 (2020-10-29)
Digital Light Processing (DLP) stereolithography (SLA) as a high-resolution 3D printing process offers a low-cost alternative for prototyping of microfluidic geometries, compared to traditional clean-room and workshop-based methods. Here, we investigate DLP-SLA printing performance for the production of micro-chamber chip
Keith E Herold et al.
Methods in molecular biology (Clifton, N.J.), 504, 441-458 (2009-01-23)
A prototype handheld, compact, rapid thermocycler was developed for multiplex analysis of nucleic acids in an inexpensive, portable configuration. Instead of the commonly used Peltier heating/cooling element, electric thin-film resistive heater and a miniature fan enable rapid heating and cooling
Monica Musiani et al.
Nature protocols, 2(10), 2502-2510 (2007-10-20)
PCR is an established technique providing rapid and highly productive amplification of specific DNA sequences. The demand for equally rapid, sensitive and objective methods to achieve detection of PCR products has led to the coupling of PCR with ELISA. PCR-ELISA
Monica Musiani et al.
Nature protocols, 2(10), 2511-2519 (2007-10-20)
Competitive PCR-ELISA combines competitive PCR with an ELISA to allow quantitative detection of PCR products. It is based on the inclusion of an internal standard competitor molecule that is designed to differ from the target by a short sequence of
Bożena Nejman-Faleńczyk et al.
Toxins, 7(11), 4745-4757 (2015-11-19)
A novel procedure for the detection of specific DNA sequences has been developed. This procedure is based on the already known method employing PCR with appropriate primers and a sequence-specific DNA probe labeled with the fluorescent agent 6-carboxylfluorescein (FAM) at
Kieu The Loan Trinh et al.
Analytica chimica acta, 1040, 63-73 (2018-10-18)
In this study, we demonstrate a simple and facile method to enable DNA purification and amplification in a continuous step inside a thermoplastic microdevice. The innate property of thermoplastics was adopted to simplify DNA purification because negatively charged DNA can
C Potrich et al.
Biomedical microdevices, 14(6), 1103-1113 (2012-07-05)
Modern Lab-on-a-chip (LOC) platforms for genomic applications integrate several biological tasks in a single device. Combination of these processes into a single device minimizes sample loss and contamination problems as well as reducing analysis time and costs. Here we present
Asensio Gonzalez et al.
Biomedical microdevices, 9(5), 729-736 (2007-05-12)
Continuous-flow analysis, where samples circulate encapsulated in a carrier fluid is an attractive alternative to batch processing for high-throughput devices that use the polymerase chain reaction (PCR). Challenges of continuous-flow prototypes include the hydrodynamic and biological incompatibility of the carrier
Asmini Athman et al.
Plant methods, 10, 29-29 (2014-09-25)
An important step in characterising the function of a gene is identifying the cells in which it is expressed. Traditional methods to determine this include in situ hybridisation, gene promoter-reporter fusions or cell isolation/purification techniques followed by quantitative PCR. These
Tobias Brandt et al.
PloS one, 7(10), e47889-e47889 (2012-11-01)
Most proteins have not evolved for maximal thermal stability. Some are only marginally stable, as for example, the DNA-binding domains of p53 and its homologs, whose kinetic and thermodynamic stabilities are strongly correlated. Here, we applied high-throughput methods using a
Peter M Wilson et al.
Nucleic acids research, 39(17), e112-e112 (2011-05-18)
Current methods for measuring deoxyribonucleoside triphosphates (dNTPs) employ reagent and labor-intensive assays utilizing radioisotopes in DNA polymerase-based assays and/or chromatography-based approaches. We have developed a rapid and sensitive 96-well fluorescence-based assay to quantify cellular dNTPs utilizing a standard real-time PCR
Thomas G W Graham et al.
Current protocols, 1(4), e130-e130 (2021-04-28)
The most common method for RNA detection involves reverse transcription followed by quantitative polymerase chain reaction (RT-qPCR) analysis. Commercial one-step master mixes-which include both a reverse transcriptase and a thermostable polymerase and thus allow performing both the RT and qPCR
Helmuth Haslacher et al.
European journal of clinical investigation, 42(5), 463-469 (2011-09-29)
Myeloperoxidase (MPO) is involved in a multitude of inflammatory processes involving oxidative modification of soluble components and cellular surfaces. Thus, MPO plays a key role in promoting atherosclerosis via oxidative stress by modification of both high- and low-density lipoprotein and
Varun L Kopparthy et al.
Biotechnology and bioengineering, 117(5), 1525-1532 (2020-01-21)
We report the development of a versatile system based on the oscillating-flow methodology in a thermal gradient system for nucleic acid analysis. Analysis of DNA and RNA samples were performed in the device, without additional temperature control and complexity. The
Zsuzsa Kreizinger et al.
PloS one, 10(7), e0133554-e0133554 (2015-07-25)
Mycoplasma synoviae infection in chickens and turkeys can cause respiratory disease, infectious synovitis and eggshell apex abnormality; thus it is an economically important pathogen. Control of M. synoviae infection comprises eradication, medication or vaccination. The differentiation of the temperature sensitive
Roberto Alcántara et al.
STAR protocols, 2(4), 100878-100878 (2021-10-05)
Here, we describe a detailed step-by-step protocol to detect SARS-CoV-2 RNA using RT-PCR-mediated amplification and CRISPR/Cas-based visualization. The optimized assay uses basic molecular biology equipment such as conventional thermocyclers and transilluminators for qualitative detection. Alternatively, a fluorescence plate reader can
F Vidal et al.
Thrombosis and haemostasis, 85(4), 580-583 (2001-05-09)
We here describe a simple, efficient DNA sequencing procedure for hemophilia A molecular diagnosis. In severe patients we first test for the presence of factor VIII gene intron 22 inversion using a recently described single-tube PCR method. In moderate, mild
Luis A Ugozzoli et al.
Molecular and cellular probes, 18(3), 161-166 (2004-05-12)
We developed a real-time multiplex four-color assay for the simultaneous detection of the factor V Leiden (FVL) and prothrombin (PT) G20210A mutations in one closed tube using a single thermocycling protocol. The assay combines the power of multiplex PCR with
Aashish Priye et al.
Methods in molecular biology (Clifton, N.J.), 1571, 251-266 (2017-03-11)
Incredible progress continues to be made toward development of low-cost nucleic acid-based diagnostic solutions suitable for deployment in resource-limited settings. Detection components play a vitally important role in these systems, but have proven challenging to adapt for operation in a
Wilfred Villariza Espulgar et al.
Sensors (Basel, Switzerland), 21(4) (2021-02-13)
Here we report the improved Cyclo olefin polymer (COP) microfluidic chip and polymerase chain reaction (PCR) amplification system for point-of-care testing (POCT) in rapid detection of Carbapenem-resistant Enterobacteriaceae (CRE). The PCR solution and thermal cycling is controlled by the relative
Yonghee Kim et al.
Micromachines, 11(2) (2020-02-23)
Influenza A viruses are often present in environmental and clinical samples at concentrations below the limit of detection (LOD) of molecular diagnostics. Here we report an integrated microfluidic preconcentration and nucleic amplification system (μFPNAS) which enables both preconcentration of influenza
Hao-Hua Sun et al.
Biotechnology progress, 25(5), 1228-1235 (2009-07-16)
Pools of short synthetic oligonucleotides (oligos) are required in the multiplex and parallel DNA construction. Microarray technology provides a fast and economical mean for massive parallel synthesis of oligos. The method of oligo synthesis with the programmable microfluidic PicoArray could
Laimutis Kucinskas et al.
World journal of gastroenterology, 14(38), 5876-5879 (2008-10-16)
To investigate the prevalence of the ATP7B gene mutation in patients with hepatic presentation of Wilson's disease (WD) in Lithuania. Eleven unrelated Lithuanian families, including 13 WD patients were tested. Clinically WD diagnosis was established in accordance to the Leipzig
Laura J Tafe et al.
Diagnostic molecular pathology : the American journal of surgical pathology, part B, 16(2), 112-115 (2007-05-26)
Classic hereditary hemochromatosis is an autosomal recessive disorder characterized by iron overload and sequence variants in the HFE gene. The HFE gene is located at 6p21.3 and contains 2 common single nucleotide polymorphisms (SNPs) C282Y and H63D, which are routinely
S T Gammon et al.
Analytical biochemistry, 369(1), 80-86 (2007-07-31)
Current methodologies for quantifying radiolabeled nucleoside monophosphates and nucleoside analogues result in high retention of unphosphorylated guanosine nucleosides in the case of lanthanum chloride precipitation or inconsistent retention of nucleotides in the case of DEAE cellulose filter papers. This study
Mathew S Box et al.
Plant methods, 7, 7-7 (2011-03-15)
Many experiments in modern plant molecular biology require the processing of large numbers of samples for a variety of applications from mutant screens to the analysis of natural variants. A severe bottleneck to many such analyses is the acquisition of
C Bertrand-Thiébault et al.
Annals of human genetics, 72(Pt 2), 178-183 (2008-01-22)
CYP2C19, a member of the cytochrome P450 family, metabolises arachidonic acid to produce epoxyeicosanoid acids, which are involved in vascular tone and inflammation. Thus, this study describes the possible relationship between a CYP2C19 polymorphism (681G>A) and three inflammatory markers: interleukin
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