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Zuo Tong How et al.
Journal of chromatography. A, 1370, 135-146 (2014-12-03)
This paper reports a new analytical method for the analysis of 18 amino acids in natural waters using solid-phase extraction (SPE) followed by liquid chromatography-electrospray tandem mass spectrometry (LC-MS/MS) operated in multiple reaction monitoring mode. Two different preconcentration methods, solid-phase
Nevi Parameswaran et al.
Virus research, 188, 68-80 (2014-04-16)
Alcelaphine herpesvirus-1 (AlHV-1) causes malignant catarrhal fever (MCF). The A2 gene of AlHV-1 is a member of the bZIP transcription factor family. We wished to determine whether A2 is a virulence gene or not and whether it is involved in
Mario Barilani et al.
Stem cells and development, 24(1), 104-114 (2014-07-22)
In regenerative medicine, human cord blood-derived multipotent mesenchymal stromal cells (CBMSCs) stand out for their biological peculiarities demonstrated in in vitro and in vivo preclinical studies. Here, we present our 9-year experience for the consistent isolation of CBMSCs. Although nearly
Capillary electrophoresis-mass spectrometry as a new approach to analyze neonicotinoid insecticides.
Laura Sánchez-Hernández et al.
Journal of chromatography. A, 1359, 317-324 (2014-08-03)
This paper represents the first report of a capillary electrophoresis (CE) method compatible with mass spectrometry (MS) detection for simultaneously analyzing seven neonicotinoid insecticides (acetamiprid, clothianidin, dinotefuran, imidacloprid, nitenpyram, thiacloprid and thiamethoxam). Different variables affecting CE separation (buffer concentration, pH
Ming-Jun Tsai et al.
International journal of pharmaceutics, 481(1-2), 84-90 (2015-01-24)
Submicron emulsion system is one kind of submicron-carrier that can ensure close contact and increase the amount of drug transport into the skin. In the present study, naringenin was loaded into a submicron emulsion system for topical applications. The enhancement
Lore Geldof et al.
Biomedical chromatography : BMC, 28(7), 974-985 (2014-02-06)
Anti-doping laboratories need to be aware of evolutions on the steroid market and elucidate steroid metabolism to identify markers of misuse. Owing to ethical considerations, in vivo and in vitro models are preferred to human excretion for nonpharmaceutical grade substances.
L Q Beach et al.
Journal of neuroendocrinology, 27(5), 324-334 (2015-02-24)
Robust sex differences in brain and behaviour exist in zebra finches. Only males sing, and forebrain song control regions are more developed in males. The factors driving these differences are not clear, although numerous experiments have shown that oestradiol (E2
Habib Bagheri et al.
Journal of chromatography. A, 1375, 8-16 (2014-12-17)
In the present work, the roles of inorganic oxide nanoparticles on the extraction efficiency of polyethylene terephthalate-based nanocomposites were extensively studied. Four fiber coatings based on polyethylene terephthalate nanocomposites containing different types of nanoparticles along with a pristine polyethylene terephthalate
Gimena Acosta et al.
Journal of pharmaceutical and biomedical analysis, 106, 79-84 (2014-10-05)
A high performance liquid chromatography coupled with atomic fluorescence spectrometry method for the determination of thimerosal (sodium ethylmercury thiosalicylate, C9H9HgNaO2S), ethylmercury, and inorganic mercury is proposed. Mercury vapor is generated by the post-column reduction of mercury species in formic acid
Wojciech Ostrowski et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 967, 21-27 (2014-07-27)
Liquid chromatography coupled to mass spectrometry (MS) with electrospray ionization (ESI) is one of analytical techniques to obtain accurate results of low molecular weight aromatic compounds in biological samples of different origin. The interpretations of mass spectra of these aromatic
Tao Tu et al.
Journal of agricultural and food chemistry, 62(52), 12686-12694 (2014-12-11)
Thermophilic endo-polygalacturonases with high catalytic efficiency are of great interest in the food and feed industries. This study identified an endo-polygalacturonase gene (pg7fn) of glycoside hydrolase family 28 in the thermophilic fungus Thielavia arenaria XZ7. Recombinant PG7fn produced in Pichia
Ivana Jovčevska et al.
PloS one, 9(11), e113688-e113688 (2014-11-25)
Malignant gliomas are among the rarest brain tumours, and they have the worst prognosis. Grade IV astrocytoma, known as glioblastoma multiforme (GBM), is a highly lethal disease where the standard therapies of surgery, followed by radiation and chemotherapy, cannot significantly
Fazren Azmi et al.
Bioorganic & medicinal chemistry, 22(22), 6401-6408 (2014-12-03)
Peptides are of great interest to be used as vaccine antigens due to their safety, ease of manufacturing and specificity in generating immune response. There have been massive discoveries of peptide antigens over the past decade. However, peptides alone are
Codrin Andrei et al.
PloS one, 9(10), e109836-e109836 (2014-10-30)
A substantial and stable increase of the current density Jsc of ruthenium (Ru) dye sensitized solar cells (DSC) of up to 16.18% and of the power efficiency of up to 25.5% is demonstrated in this article via plasmonic enhancement. The
Gunnel H Nilsson et al.
Journal of analytical toxicology, 38(6), 327-334 (2014-05-03)
A simple liquid chromatography-tandem mass spectrometry method was validated to allow determination of zopiclone (ZOP), N-desmethylzopiclone (NDZOP), zopiclone N-oxide (ZOPNO) and 2-amino-5-chloropyridine (ACP) in urine at concentrations up to 3,000 ng/mL within 3.5 min. This method was used for quantitative
Ehab Mostafa et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 15(10), 2029-2043 (2014-07-06)
The adsorption and oxidation of methanol at Pt(331) and Ru-step-decorated Pt(331) electrodes are studied recording currents and ion currents by online differential electrochemical mass spectrometry. The CO(2) current efficiencies and the degree of surface poisoning with CO(ad) formed during methanol
B Madikizela et al.
Journal of ethnopharmacology, 156, 228-234 (2014-09-15)
The emergence of drug resistant-tuberculosis and other pathogenic diseases over the past decades, constitutes a serious threat to human health worldwide. According to a 2012 report by the World Health Organization (WHO), South Africa, China, India and Russia are the
Gimena dos Santos et al.
Nature communications, 6, 6574-6574 (2015-03-13)
Activation of the NLRP3 inflammasome and subsequent maturation of IL-1β have been implicated in acute lung injury (ALI), resulting in inflammation and fibrosis. We investigated the role of vimentin, a type III intermediate filament, in this process using three well-characterized
Nhung Dang et al.
Journal of pharmaceutical sciences, 103(7), 2107-2115 (2014-05-29)
Nevirapine (NVP) was loaded in polycaprolactone (PCL) matrices to produce vaginal inserts with the aim of preventing HIV transmission. NVP dispersions in PCL were prepared, at 10% (w/w) theoretical loading, measured with respect to the PCL content of the matrices
Kyung-Soo Nam et al.
Oncology letters, 9(1), 483-487 (2014-12-02)
Colorectal adenocarcinoma is the most common type of gastrointestinal cancer. Colon adenocarcinoma is a major health problem worldwide due to the high prevalence and mortality rates associated with the disease. The majority of colorectal carcinomas are adenocarcinomas, which originate from
Michele Protti et al.
Journal of pharmaceutical and biomedical analysis, 104, 75-80 (2014-12-09)
Chinotto (Citrus×myrtifolia) is a uncommon fruit belonging to the Citrus genus, mainly cultivated in small areas of the Italian territory, where the main use concerns the eponymous drink, marketed with the name of "Chinotto". The lack of information about this
Lenka Šubčíková et al.
Journal of chromatography. A, 1378, 8-18 (2015-01-04)
Fatty acid diesters of long-chain 1,2-diols (1,2-DDE), or type II wax diesters, were analyzed in the vernix caseosa of a newborn girl. 1,2-DDE were isolated from the total lipid extract by the semipreparative TLC using plates coated with silica gel.
Jeong Yeon Kang et al.
Pharmaceutical research, 32(3), 852-862 (2014-08-28)
Cardiotoxicity associated with the use of doxorubicin (DOX), and other chemotherapeutics, limits their clinical potential. This study determined the pharmacokinetics and antitumor and cardioprotective activity of free and liposome encapsulated phenyl-2-aminoethyl-selenide (PAESe). The pharmacokinetics of free PAESe and PAESe encapsulated
Ruoqi Zhang et al.
Journal of chromatographic science, 52(10), 1151-1156 (2014-04-10)
In this study, a simple and selective gas chromatography-mass spectrometry method was developed and validated for the determination of pogostone in canine plasma. Liquid-liquid extraction was used to separate pogostone from canine plasma, and the mean extraction recovery rates of
M Roca et al.
Journal of chromatography. A, 1374, 66-76 (2014-12-17)
In this study we propose an analytical strategy that combines a target approach for the quantitative analysis of contemporary pesticide metabolites with a comprehensive post-target screening for the identification of biomarkers of exposure to environmental contaminants in urine using liquid
Feifan Xie et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 960, 98-104 (2014-05-06)
A simple, sensitive and cost-effective assay based on reversed phase high performance liquid chromatography (RP-HPLC) with isocratic mode for simultaneous determination of bendamustine (BM) and its active metabolite, gamma-hydroxy-bendamustine (γ-OH-BM) in human plasma and urine was developed and validated. Sample
Xiong Li et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 962, 37-43 (2014-06-03)
A rapid, sensitive and selective UPLC/MS method using LTQ Orbitrap mass spectrometry was established for the analysis and characterization of the main biological components and their metabolites in rat plasma, urine and feces following oral administration of Lithocarpus polystachyus extract.
Xiaoye He et al.
Food chemistry, 171, 405-411 (2014-10-14)
A simple and reliable HPLC fingerprint method was developed and validated for the quality control and identification of Ziyang green tea. Ten batches of Ziyang green tea collected from different plantations in Shaanxi Ziyang of China were used to establish
Anna M Piasta et al.
Analytica chimica acta, 834, 58-66 (2014-06-15)
A new procedure for determination of biogenic amines (BA): histamine, phenethylamine, tyramine and tryptamine, based on the derivatization reaction with 2-chloro-1,3-dinitro-5-(trifluoromethyl)-benzene (CNBF), is proposed. The amines derivatives with CNBF were isolated and characterized by X-ray crystallography and (1)H, (13)C, (19)F
Junhua Wang et al.
Molecular medicine reports, 10(6), 3009-3014 (2014-10-14)
Crotoxin (CrTX) is a neurotoxin isolated from the venom of the South American rattlesnake. Previous studies demonstrated that CrTX was able to inhibit the activity of the growth factor receptor tyrosine kinase and that CrTX possesses potent antitumor activity when
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