Buffers
Buffer A:
0.02 M NaH2PO4 (such as Cat. No. S3139): 2.4 g
0.15 M NaCl (such as Cat. No. S3014): 8.8 g
Adjust volume to 1 liter with water. Adjust pH to 8.0.
Buffer B:
Buffers
Buffer A:
0.02 M NaH2PO4 (such as Cat. No. S3139): 2.4 g
0.15 M NaCl (such as Cat. No. S3014): 8.8 g
Adjust volume to 1 liter with water. Adjust pH to 8.0.
Buffers
Buffer A:
0.02 M NaH2PO4 (such as Cat. No. S3139): 2.4 g
0.15 M NaCl (such as Cat. No. S3014): 8.8 g
Adjust volume to 1 liter with water. Adjust pH to 8.0.
Buffer B:
Buffers
Buffer A:
0.02 M NaH2PO4 (such as Cat. No. S3139): 2.4 g
0.15 M NaCl (such as Cat. No. S3014): 8.8 g
Adjust volume to 1 liter with water. Adjust pH to 8.0.
Buffer B:
Buffers
Buffer A:
0.02 M NaH2PO4 (such as Cat. No. S3139): 2.4 g
0.15 M NaCl (such as Cat. No. S3014): 8.8 g
Adjust volume to 1 liter with water. Adjust pH to 8.0.
(Cat. No. C1990)
• Imidazole, such as Cat. No. I5513
• Sodium chloride, such as Cat. No. S3014
• Sodium phosphate, such as Cat. No. S0751
(monobasic) and Cat. No. S3264 (dibasic)
(Cat. No. C1990)
• Imidazole, such as Cat. No. I5513
• Sodium chloride, such as Cat. No. S3014
• Sodium phosphate, such as Cat. No. S3139
(monobasic) and Cat. No. S3264 (dibasic)
Buffers
Buffer A:
0.02 M NaH2PO4 (such as Cat. No. S3139): 2.4 g
0.15 M NaCl (such as Cat. No. S3014): 8.8 g
Adjust volume to 1 liter with water. Adjust pH to 8.0.
Buffers
Buffer A:
0.02 M NaH2PO4 (such as Cat. No. S3139): 2.4 g
0.15 M NaCl (such as Cat. No. S3014): 8.8 g
Adjust volume to 1 liter with water. Adjust pH to 8.0.
Buffer B:
Imidazole, such as Cat. No. I5513 (for molecular
biology)
• Sodium chloride, such as Cat. No. S3014 (for
molecular biology)
• Sodium phosphate, such as Cat. No. S3139
(monobasic, BioReagent
1 M NaCl
1. For 100 ml, mix 20 g PEG 8000 (Product
No. P5413) and 5.84 g of NaCl (Product No. S3014)
in 100 ml of water.
2. Autoclave 20 minutes at 15 lbs/sq. in.
3. While the
, such as Cat. No. I5513
(for molecular biology)
• Sodium chloride (NaCl), such as Cat. No. S3014
(for molecular biology)
• Sodium phosphate, such as Cat. No. S3139
(monobasic, for molecular
Imidazole, such as Cat. No. I5513 (for molecular
biology)
• Sodium chloride (NaCl), such as Cat. No. S3014
(for molecular biology)
• Sodium phosphate, such as Cat. No. S3139
(monobasic, for molecular
CelLytic B II, Product No. B3678
· Appropriate column or centrifuge tubes
· Sodium chloride, Product No. S3014
· Trizma® base, Product No T6066
· Protease inhibitor cocktail for use with mammalian cells and tissue