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Pamela Sarkar et al.
Multiple sclerosis (Houndmills, Basingstoke, England), 13524585211060686-13524585211060686 (2021-11-30)
Cell-based therapies for multiple sclerosis (MS), including those employing autologous bone marrow-derived mesenchymal stromal cells (MSC) are being examined in clinical trials. However, recent studies have identified abnormalities in the MS bone marrow microenvironment. We aimed to compare the secretome
Priti Azad et al.
The Journal of clinical investigation, 133(11) (2023-04-07)
Excessive erythrocytosis (EE) is a major hallmark of patients suffering from chronic mountain sickness (CMS, also known as Monge's disease) and is responsible for major morbidity and even mortality in early adulthood. We took advantage of unique populations, one living
M Leslie Fulcher et al.
Methods in molecular biology (Clifton, N.J.), 945, 109-121 (2012-10-26)
Human airway epithelial (hAE) cell cultures are instrumental for studying basic and applied aspects of respiratory tract biology, disease, and therapy. When primary epithelial cells from the human nasal passages or tracheo-bronchial airways are grown on porous supports at an
Senthil Raja Jayapal et al.
Cell cycle (Georgetown, Tex.), 15(22), 3070-3081 (2016-09-23)
Cyclin A2 is an essential gene for development and in haematopoietic stem cells and therefore its functions in definitive erythropoiesis have not been investigated. We have ablated cyclin A2 in committed erythroid progenitors in vivo using erythropoietin receptor promoter-driven Cre
Angelo Michele Lavecchia et al.
MethodsX, 9, 101753-101753 (2022-06-28)
Insufficient production of erythropoietin (EPO) leads to anaemia. Developing methods for the generation and transplantation of EPO-producing cells would allow scientists to design personalised therapeutic solutions. Here we present a simple and highly reproducible protocol for the generation of neural
Alex Moore et al.
Frontiers in neuroscience, 16, 832961-832961 (2022-04-26)
Hypothalamic tanycytes are neural stem and progenitor cells, but little is known of how they are regulated. Here we provide evidence that the cell adhesion molecule, NrCAM, regulates tanycytes in the adult niche. NrCAM is strongly expressed in adult mouse
Xiaoxing Liang et al.
Journal of hazardous materials, 407, 124387-124387 (2020-11-12)
The widely used chemical bisphenol A (BPA) has been associated with several health effects. In recent years, many derivatives were developed to replace BPA although without thorough toxicological evaluation. Here, we employed a human embryoid body (EB)-based in vitro global
Gad D Vatine et al.
Cell stem cell, 24(6), 995-1005 (2019-06-08)
The blood-brain barrier (BBB) tightly regulates the entry of solutes from blood into the brain and is disrupted in several neurological diseases. Using Organ-Chip technology, we created an entirely human BBB-Chip with induced pluripotent stem cell (iPSC)-derived brain microvascular endothelial-like cells
Xiuyue Song et al.
Journal of tissue engineering, 13, 20417314221125310-20417314221125310 (2022-09-30)
Endothelial cells (ECs) usually form a monolayer on two-dimensional (2D) stiff substrates and a tubular structure with soft hydrogels. The coculture models using ECs and pericytes derived from different adult tissues or pluripotent stem cells cannot mimic tissue-specific microvessels due
Over One Million DNA and Protein Events Through Ultra-Stable Chemically-Tuned Solid-State Nanopores.
Jugal Saharia et al.
Small (Weinheim an der Bergstrasse, Germany), 19(29), e2300198-e2300198 (2023-04-08)
Stability, long lifetime, resilience against clogging, low noise, and low cost are five critical cornerstones of solid-state nanopore technology. Here, a fabrication protocol is described wherein >1 million events are obtained from a single solid-state nanopore with both DNA and
Rei-Lin Kuo et al.
RNA biology, 18(5), 796-808 (2021-01-08)
The pathogenic human enterovirus EV-A71 has raised serious public health concerns. A hallmark of EV-A71 infection is the distortion of host transcriptomes in favour of viral replication. While high-throughput approaches have been exploited to dissect these gene dysregulations, they do not
Jugal Saharia et al.
Electrophoresis, 41(7-8), 630-637 (2019-11-12)
In this work, we present a step-by-step workflow for the fabrication of 2D hexagonal boron nitride (h-BN) nanopores which are then used to sense holo-human serum transferrin (hSTf) protein at pH ∼8 under applied voltages ranging from +100 mV to +800 mV.
Dick J Sjöström et al.
The FEBS journal, 289(10), 2935-2947 (2021-12-05)
Human transferrin receptor 1 (TfR) is necessary for the delivery of the iron carrier protein transferrin into cells and can be utilized for targeted delivery across cellular membranes. Binding of transferrin to the receptor is regulated by hereditary hemochromatosis protein
Xiaoxing Liang et al.
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 135, 111015-111015 (2019-12-10)
Bisphenol A (BPA) and its derivatives, including bisphenols S (BPS), F (BPF), E (BPE), B (BPB), Z (BPZ), and AF (BPAF), are widely used in consumer products. Moreover, they are typically detected in the environment, food, and humans. Previous studies
Divya S Vinjamur et al.
Nature genetics, 53(5), 719-728 (2021-04-17)
Known fetal hemoglobin (HbF) silencers have potential on-target liabilities for rational β-hemoglobinopathy therapeutic inhibition. Here, through transcription factor (TF) CRISPR screening, we identify zinc-finger protein (ZNF) 410 as an HbF repressor. ZNF410 does not bind directly to the genes encoding
Yalan Xu et al.
Stem cell research & therapy, 13(1), 299-299 (2022-07-16)
Most castration-resistant prostate cancers (CRPCs) have a luminal phenotype with high androgen receptor (AR) and prostate-specific antigen (PSA) expression. Currently, it is difficult to culture castration-resistant luminal cells with AR and PSA expression. We formulated a custom-made medium and isolated
Yang Yang et al.
Cell reports, 29(11), 3374-3384 (2019-12-12)
During embryogenesis, various cell types emerge simultaneously from their common progenitors under the influence of intrinsic signals. Human embryonic stem cells can differentiate to diverse cell types of three embryonic lineages, making them an excellent system for understanding the regulatory
Kun Wang et al.
Cell, 180(5), 941-955 (2020-02-29)
The pyroptosis execution protein GSDMD is cleaved by inflammasome-activated caspase-1 and LPS-activated caspase-11/4/5. The cleavage unmasks the pore-forming domain from GSDMD-C-terminal domain. How the caspases recognize GSDMD and its connection with caspase activation are unknown. Here, we show site-specific caspase-4/11
Sandra Pilat et al.
Methods in molecular biology (Clifton, N.J.), 1029, 129-147 (2013-06-13)
Differentiation of pluripotent embryonic stem (ES) cells can recapitulate many aspects of hematopoiesis, in vitro, and can even generate cells capable of long-term multilineage repopulation after transplantation into recipient mice, when the homeodomain transcription factor HOXB4 is ectopically expressed. Thus
Jugal Saharia et al.
Electrophoresis, 43(5-6), 785-792 (2022-01-13)
Electrolyte chemistry plays an important role in the transport properties of analytes through nanopores. Here, we report the translocation properties of the protein human serum transferrin (hSTf) in asymmetric LiCl salt concentrations with either positive (Ctrans /Ccis < 1) or
David B Hill et al.
Methods in molecular biology (Clifton, N.J.), 842, 245-258 (2012-01-20)
Primary cultures of human airway bronchial airways represent a valuable tool in understanding the roles of the epithelium, cilia, and the mucus layer in coordinating the clearance of mucus from the airways. The ability to obtain cells from both normal
Allison L Fisher et al.
American journal of hematology, 97(12), 1548-1559 (2022-09-08)
Systemic iron homeostasis is regulated by the hepatic hormone hepcidin to balance meeting iron requirements while limiting toxicity from iron excess. Iron-mediated induction of bone morphogenetic protein (BMP) 6 is a central mechanism for regulating hepcidin production. Liver endothelial cells
Yasir Ahmed Syed et al.
Methods in molecular biology (Clifton, N.J.), 1493, 363-378 (2016-10-28)
Oligodendrocytes are the myelinating cells of the central nervous system. The role of oligodendrocytes in health and disease has been considerably enhanced by the development of methods to isolate and culture oligodendrocytes from central nervous system tissue. The cellular and
Jugal Saharia et al.
RSC advances, 11(39), 24398-24409 (2021-08-07)
Nanopore probing of molecular level transport of proteins is strongly influenced by electrolyte type, concentration, and solution pH. As a result, electrolyte chemistry and applied voltage are critical for protein transport and impact, for example, capture rate (C R), transport
Faxiang Xu et al.
STAR protocols, 2(3), 100740-100740 (2021-09-02)
Metabolic homeostasis is critical for cell pluripotency and differentiation in human embryonic stem cells (hESCs). It has been reported that metabolic changes specifically regulate cellular signaling during hESC differentiation. This protocol describes procedures for both cell culture and detection of
Matthildi Valianou et al.
Cell cycle (Georgetown, Tex.), 14(3), 399-407 (2015-01-08)
The mechanistic target of rapamycin complex 1 (mTORC1) increases translation, cell size and angiogenesis, and inhibits autophagy. mTORC1 is negatively regulated by hamartin and tuberin, the protein products of the tumor suppressors TSC1 and TSC2 that are mutated in Tuberous
Joe N Frost et al.
Med (New York, N.Y.), 2(2), 164-179 (2021-03-06)
How specific nutrients influence adaptive immunity is of broad interest. Iron deficiency is the most common micronutrient deficiency worldwide and imparts a significant burden of global disease; however, its effects on immunity remain unclear. We used a hepcidin mimetic and
Stefano Menghini et al.
International journal of molecular sciences, 22(2) (2021-01-10)
Interest has grown in harnessing biological agents for cancer treatment as dynamic vectors with enhanced tumor targeting. While bacterial traits such as proliferation in tumors, modulation of an immune response, and local secretion of toxins have been well studied, less
Hilal Varinli et al.
Journal of lipid research, 56(11), 2206-2216 (2015-09-04)
Lipid droplets (LDs) are the main storage organelles for triglycerides. Elucidation of lipid accumulation mechanisms and metabolism are essential to understand obesity and associated diseases. Adipogenesis has been well studied in murine 3T3-L1 and human Simpson-Golabi-Behmel syndrome (SGBS) preadipocyte cell
Maira Di Tano et al.
Nature communications, 11(1), 2332-2332 (2020-05-13)
Fasting-mimicking diets delay tumor progression and sensitize a wide range of tumors to chemotherapy, but their therapeutic potential in combination with non-cytotoxic compounds is poorly understood. Here we show that vitamin C anticancer activity is limited by the up-regulation of
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